Standard name
Human Ortholog
Description Karyopherin beta; responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.11 0 0.11 0.09 0.16 0.16 0.13 0.17 0.15 0.17 0.16 0.15 0.24 0.26 0.17 0.2 0.15 0 0 0 0.05 0.06 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.14 0.22
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.08 0 0.21 0.3 0.37 0.46 0.51 0.6 0.54 0 0 0 0.05 0 0 0 0 0 0 0.07 0.07
Nucleus 0.17 0.16 0.06 0.16 0.15 0.15 0.18 0.17 0.18 0.19 0.2 0.17 0.15 0.09 0.17 0.27 0.25 0.15 0.16 0.2 0.08 0.1 0.07 0.06
Nuclear Periphery 0.12 0.1 0.19 0.25 0.15 0.09 0.11 0.08 0.1 0.09 0.05 0.08 0 0 0 0.16 0.11 0.09 0.58 0.46 0.66 0.39 0.26 0.17
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Vac/Vac Membrane 0.8 0.74 0.79 0.57 0.68 0.52 0.54 0.42 0.36 0.32 0.23 0.33 0.64 0.71 0.65 0.52 0.53 0.72 0.12 0.13 0.1 0.2 0.23 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 2 0 1 0
Bud 0 0 0 0 1 0 0 8 2 2 7 7 0 0 0 2 1 0 3 5 3 2 9 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 2 0 0 3 8 5 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 20 16 10 24 21 55 68 71 46 74 94 105 5 35 32 86 76 55 6 5 3 6 14 16
Endoplasmic Reticulum 4 1 0 2 0 2 1 0 2 2 1 1 2 0 6 20 16 21 2 3 6 1 11 7
Endosome 1 1 10 11 17 12 11 24 5 5 4 17 0 1 2 7 9 13 10 7 16 9 37 45
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 3 0 1 4 4
Mitochondria 3 7 2 17 4 71 131 207 123 261 338 346 0 0 0 26 12 7 4 1 2 5 18 13
Nucleus 53 23 17 36 34 52 77 92 49 98 113 112 5 13 21 135 97 58 58 32 22 11 19 13
Nuclear Periphery 38 15 51 55 34 32 49 46 26 45 30 50 1 3 0 80 42 34 215 75 199 48 68 36
Nucleolus 3 1 2 1 1 4 1 15 3 5 4 7 0 0 0 1 3 4 9 3 4 1 3 2
Peroxisomes 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 0 0 0 0 3 2 3 0 0 0 0 0 1 7 2 7 7 8 7
Vac/Vac Membrane 248 106 210 125 153 175 236 230 96 163 131 210 21 102 80 254 203 271 44 22 31 25 62 49
Unique Cell Count 310 143 267 221 225 337 434 553 270 509 561 646 33 144 124 492 382 378 369 165 304 124 269 213
Labelled Cell Count 370 170 304 273 265 405 574 695 355 667 730 864 34 155 141 612 460 466 369 165 304 124 269 213


Cytoplasm, Nuclear Periphery, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 7.9 8.0 7.6 7.3 5.8 5.5 5.4 5.1 4.7 4.7 4.8 7.4 8.3 8.0 6.9 7.7 9.8 8.3 8.8 9.5
Std Deviation (1e-4) 1.5 1.6 1.4 1.9 1.7 1.4 1.3 1.3 1.3 1.2 1.2 1.3 1.0 1.3 1.2 1.3 1.7 2.3 1.3 1.4 1.7
Intensity Change (Log2) -0.08 -0.15 -0.47 -0.55 -0.57 -0.64 -0.76 -0.77 -0.73 -0.12 0.04 -0.01 -0.21 -0.06 0.28 0.05 0.14 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.1 2.5 5.0 4.9 4.1 5.0 4.6 5.3 5.2 0 6.4 6.6 5.4 6.0 4.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.3 3.4 3.9
Endosome 0.7 1.8 -0.1 -0.9 0.4 -1.3 0 0 0 0 0 0 -2.1 -1.0 -0.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.9 0 7.6 9.7 11.3 12.3 0 0 0 0 0 0 3.2 2.1 0
Nucleus 3.5 3.2 3.5 4.3 4.1 4.2 4.8 5.1 4.3 0 1.0 3.3 6.9 6.3 3.5
Nuclear Periphery 1.5 -1.2 -3.4 -2.9 -4.5 -3.1 0 0 0 -2.3 -4.9 -5.2 -1.0 -2.9 -3.7
Nucleolus 0 0 0 0 1.8 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -5.2 -2.7 -6.8 -6.5 -10.0 -10.1 -12.4 -15.1 -12.7 -1.9 -1.8 -3.0 -7.3 -6.6 -2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3091 4.6517 4.1089 3.6392 3.363 3.7633 4.7774 5.8667 5.9138 5.4666 5.5725 5.1985 2.1485 5.8395 5.5855 6.1707 5.4584 5.4216
Actin 0.0102 0.0005 0.0347 0.0039 0.0325 0.0009 0.035 0.0003 0.0039 0.0013 0.0022 0.0018 0.0045 0.0005 0.0014 0.0021 0.0004 0.0015
Bud 0.0017 0.0001 0.0004 0.0001 0.0005 0 0.0004 0.0003 0.0001 0 0.0005 0.0001 0.0004 0 0 0 0 0.0001
Bud Neck 0.0008 0.0003 0.0035 0.0006 0.0014 0.0006 0.0004 0.0006 0.0001 0.0001 0.0042 0.0011 0.0026 0.0001 0.0002 0.0002 0.0033 0.0008
Bud Periphery 0.0025 0 0.0005 0.0001 0.0006 0.0001 0.0011 0.0002 0 0 0.0004 0.0003 0.0005 0 0 0.0001 0.0001 0.0002
Bud Site 0.0062 0.001 0.005 0.0005 0.0016 0 0.0006 0.0019 0.0007 0.0001 0.0023 0.0001 0.0052 0.0001 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0005 0.0001 0.0002 0 0.0005 0 0.0001 0 0 0 0.0001 0 0.0021 0 0 0.0001 0.0001 0.0001
Cytoplasm 0.0128 0.0288 0.0365 0.0085 0.0203 0.0126 0.0156 0.013 0.0289 0.0467 0.0405 0.0072 0.0086 0.0283 0.0555 0.0139 0.043 0.0158
Cytoplasmic Foci 0.0085 0.0057 0.0162 0.0273 0.0459 0.0023 0.0046 0.0037 0.0127 0.0005 0.0225 0.0023 0.0892 0.0002 0.0003 0.0038 0.0002 0.0003
Eisosomes 0.0002 0 0.0003 0 0.0001 0 0.0001 0 0 0 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0168 0.0099 0.0128 0.0083 0.0509 0.0042 0.0201 0.0248 0.0174 0.0385 0.0152 0.0114 0.0023 0.0315 0.0462 0.0254 0.0207 0.0478
Endosome 0.0758 0.0135 0.0331 0.0176 0.0807 0.0045 0.0283 0.0129 0.0112 0.0033 0.071 0.0123 0.14 0.0052 0.0052 0.0446 0.0078 0.0078
Golgi 0.0073 0.0006 0.0173 0.0118 0.0182 0.0049 0.0048 0.0007 0.0026 0.0001 0.041 0.0067 0.0161 0 0.0001 0.0009 0.0001 0.0004
Lipid Particles 0.0069 0.0034 0.0102 0.0037 0.0274 0.0033 0.0354 0.0006 0.0014 0.0001 0.0029 0.0038 0.2372 0.0001 0.0001 0.0157 0.0001 0.0006
Mitochondria 0.0086 0.0005 0.0554 0.0016 0.0038 0.0012 0.0043 0.0007 0.0014 0.0005 0.0062 0.0029 0.0028 0.0004 0.0007 0.001 0.0008 0.0063
None 0.011 0.0005 0.0171 0.0003 0.0044 0.0006 0.0057 0.0003 0.0013 0.0056 0.0375 0.01 0.0144 0.0003 0.0023 0.0039 0.0011 0.001
Nuclear Periphery 0.5237 0.5372 0.4411 0.4687 0.3979 0.4647 0.6482 0.683 0.7293 0.8007 0.5273 0.6472 0.0354 0.7417 0.644 0.681 0.5297 0.5331
Nucleolus 0.0084 0.0016 0.0005 0.0003 0.0022 0.0006 0.0017 0.0006 0.0002 0.0001 0.0026 0.0022 0.1482 0.0002 0.0003 0.0006 0.0011 0.0011
Nucleus 0.2392 0.3853 0.2897 0.4356 0.2394 0.4885 0.1653 0.2385 0.1609 0.0904 0.1876 0.28 0.0239 0.1751 0.2306 0.1829 0.3671 0.3679
Peroxisomes 0.0018 0.0002 0.0085 0.0042 0.013 0.0025 0.0035 0.0005 0.0155 0 0.0037 0.0004 0.0188 0 0 0.0003 0 0
Punctate Nuclear 0.0123 0.0027 0.0034 0.0017 0.0267 0.0014 0.0062 0.0039 0.0019 0.0013 0.0204 0.0026 0.1939 0.0028 0.0041 0.0104 0.0023 0.0041
Vacuole 0.0329 0.0045 0.0058 0.0007 0.0215 0.0032 0.0081 0.0062 0.0017 0.0013 0.0051 0.0024 0.0482 0.0061 0.0021 0.0064 0.0154 0.0034
Vacuole Periphery 0.0118 0.0037 0.0079 0.0044 0.0102 0.0039 0.0104 0.0074 0.0087 0.0095 0.0067 0.0051 0.0057 0.0071 0.0067 0.0064 0.0065 0.0076

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.8983 37.6804 29.3134 36.6016 35.4788 26.1599 32.7986 25.403 24.5718 41.8372
Translational Efficiency 0.5809 0.6476 0.7325 0.5455 0.608 0.929 0.5271 0.5808 0.5693 0.5259

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Karyopherin beta; responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance
Localization
Cell Percentages nuclear periphery (55%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kap122

Kap122


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kap122-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available