Standard name | |
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Human Ortholog | |
Description | Karyopherin beta; responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 8 | 2 | 2 | 7 | 7 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 5 | 3 | 2 | 9 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 8 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 20 | 16 | 10 | 24 | 21 | 55 | 68 | 71 | 46 | 74 | 94 | 105 | 5 | 35 | 32 | 86 | 76 | 55 | 6 | 5 | 3 | 6 | 14 | 16 |
Endoplasmic Reticulum | 4 | 1 | 0 | 2 | 0 | 2 | 1 | 0 | 2 | 2 | 1 | 1 | 2 | 0 | 6 | 20 | 16 | 21 | 2 | 3 | 6 | 1 | 11 | 7 |
Endosome | 1 | 1 | 10 | 11 | 17 | 12 | 11 | 24 | 5 | 5 | 4 | 17 | 0 | 1 | 2 | 7 | 9 | 13 | 10 | 7 | 16 | 9 | 37 | 45 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 1 | 4 | 4 |
Mitochondria | 3 | 7 | 2 | 17 | 4 | 71 | 131 | 207 | 123 | 261 | 338 | 346 | 0 | 0 | 0 | 26 | 12 | 7 | 4 | 1 | 2 | 5 | 18 | 13 |
Nucleus | 53 | 23 | 17 | 36 | 34 | 52 | 77 | 92 | 49 | 98 | 113 | 112 | 5 | 13 | 21 | 135 | 97 | 58 | 58 | 32 | 22 | 11 | 19 | 13 |
Nuclear Periphery | 38 | 15 | 51 | 55 | 34 | 32 | 49 | 46 | 26 | 45 | 30 | 50 | 1 | 3 | 0 | 80 | 42 | 34 | 215 | 75 | 199 | 48 | 68 | 36 |
Nucleolus | 3 | 1 | 2 | 1 | 1 | 4 | 1 | 15 | 3 | 5 | 4 | 7 | 0 | 0 | 0 | 1 | 3 | 4 | 9 | 3 | 4 | 1 | 3 | 2 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 2 | 7 | 7 | 8 | 7 |
Vac/Vac Membrane | 248 | 106 | 210 | 125 | 153 | 175 | 236 | 230 | 96 | 163 | 131 | 210 | 21 | 102 | 80 | 254 | 203 | 271 | 44 | 22 | 31 | 25 | 62 | 49 |
Unique Cell Count | 310 | 143 | 267 | 221 | 225 | 337 | 434 | 553 | 270 | 509 | 561 | 646 | 33 | 144 | 124 | 492 | 382 | 378 | 369 | 165 | 304 | 124 | 269 | 213 |
Labelled Cell Count | 370 | 170 | 304 | 273 | 265 | 405 | 574 | 695 | 355 | 667 | 730 | 864 | 34 | 155 | 141 | 612 | 460 | 466 | 369 | 165 | 304 | 124 | 269 | 213 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.8 | 7.9 | 8.0 | 7.6 | 7.3 | 5.8 | 5.5 | 5.4 | 5.1 | 4.7 | 4.7 | 4.8 | 7.4 | 8.3 | 8.0 | 6.9 | 7.7 | 9.8 | 8.3 | 8.8 | 9.5 |
Std Deviation (1e-4) | 1.5 | 1.6 | 1.4 | 1.9 | 1.7 | 1.4 | 1.3 | 1.3 | 1.3 | 1.2 | 1.2 | 1.3 | 1.0 | 1.3 | 1.2 | 1.3 | 1.7 | 2.3 | 1.3 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.15 | -0.47 | -0.55 | -0.57 | -0.64 | -0.76 | -0.77 | -0.73 | -0.12 | 0.04 | -0.01 | -0.21 | -0.06 | 0.28 | 0.05 | 0.14 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.1 | 2.5 | 5.0 | 4.9 | 4.1 | 5.0 | 4.6 | 5.3 | 5.2 | 0 | 6.4 | 6.6 | 5.4 | 6.0 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.4 | 3.9 |
Endosome | 0.7 | 1.8 | -0.1 | -0.9 | 0.4 | -1.3 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | -1.0 | -0.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.9 | 0 | 7.6 | 9.7 | 11.3 | 12.3 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 2.1 | 0 |
Nucleus | 3.5 | 3.2 | 3.5 | 4.3 | 4.1 | 4.2 | 4.8 | 5.1 | 4.3 | 0 | 1.0 | 3.3 | 6.9 | 6.3 | 3.5 |
Nuclear Periphery | 1.5 | -1.2 | -3.4 | -2.9 | -4.5 | -3.1 | 0 | 0 | 0 | -2.3 | -4.9 | -5.2 | -1.0 | -2.9 | -3.7 |
Nucleolus | 0 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -5.2 | -2.7 | -6.8 | -6.5 | -10.0 | -10.1 | -12.4 | -15.1 | -12.7 | -1.9 | -1.8 | -3.0 | -7.3 | -6.6 | -2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.3091 | 4.6517 | 4.1089 | 3.6392 | 3.363 | 3.7633 | 4.7774 | 5.8667 | 5.9138 | 5.4666 | 5.5725 | 5.1985 | 2.1485 | 5.8395 | 5.5855 | 6.1707 | 5.4584 | 5.4216 |
Actin | 0.0102 | 0.0005 | 0.0347 | 0.0039 | 0.0325 | 0.0009 | 0.035 | 0.0003 | 0.0039 | 0.0013 | 0.0022 | 0.0018 | 0.0045 | 0.0005 | 0.0014 | 0.0021 | 0.0004 | 0.0015 |
Bud | 0.0017 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0 | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Neck | 0.0008 | 0.0003 | 0.0035 | 0.0006 | 0.0014 | 0.0006 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0042 | 0.0011 | 0.0026 | 0.0001 | 0.0002 | 0.0002 | 0.0033 | 0.0008 |
Bud Periphery | 0.0025 | 0 | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0.0011 | 0.0002 | 0 | 0 | 0.0004 | 0.0003 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 |
Bud Site | 0.0062 | 0.001 | 0.005 | 0.0005 | 0.0016 | 0 | 0.0006 | 0.0019 | 0.0007 | 0.0001 | 0.0023 | 0.0001 | 0.0052 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0021 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0128 | 0.0288 | 0.0365 | 0.0085 | 0.0203 | 0.0126 | 0.0156 | 0.013 | 0.0289 | 0.0467 | 0.0405 | 0.0072 | 0.0086 | 0.0283 | 0.0555 | 0.0139 | 0.043 | 0.0158 |
Cytoplasmic Foci | 0.0085 | 0.0057 | 0.0162 | 0.0273 | 0.0459 | 0.0023 | 0.0046 | 0.0037 | 0.0127 | 0.0005 | 0.0225 | 0.0023 | 0.0892 | 0.0002 | 0.0003 | 0.0038 | 0.0002 | 0.0003 |
Eisosomes | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0168 | 0.0099 | 0.0128 | 0.0083 | 0.0509 | 0.0042 | 0.0201 | 0.0248 | 0.0174 | 0.0385 | 0.0152 | 0.0114 | 0.0023 | 0.0315 | 0.0462 | 0.0254 | 0.0207 | 0.0478 |
Endosome | 0.0758 | 0.0135 | 0.0331 | 0.0176 | 0.0807 | 0.0045 | 0.0283 | 0.0129 | 0.0112 | 0.0033 | 0.071 | 0.0123 | 0.14 | 0.0052 | 0.0052 | 0.0446 | 0.0078 | 0.0078 |
Golgi | 0.0073 | 0.0006 | 0.0173 | 0.0118 | 0.0182 | 0.0049 | 0.0048 | 0.0007 | 0.0026 | 0.0001 | 0.041 | 0.0067 | 0.0161 | 0 | 0.0001 | 0.0009 | 0.0001 | 0.0004 |
Lipid Particles | 0.0069 | 0.0034 | 0.0102 | 0.0037 | 0.0274 | 0.0033 | 0.0354 | 0.0006 | 0.0014 | 0.0001 | 0.0029 | 0.0038 | 0.2372 | 0.0001 | 0.0001 | 0.0157 | 0.0001 | 0.0006 |
Mitochondria | 0.0086 | 0.0005 | 0.0554 | 0.0016 | 0.0038 | 0.0012 | 0.0043 | 0.0007 | 0.0014 | 0.0005 | 0.0062 | 0.0029 | 0.0028 | 0.0004 | 0.0007 | 0.001 | 0.0008 | 0.0063 |
None | 0.011 | 0.0005 | 0.0171 | 0.0003 | 0.0044 | 0.0006 | 0.0057 | 0.0003 | 0.0013 | 0.0056 | 0.0375 | 0.01 | 0.0144 | 0.0003 | 0.0023 | 0.0039 | 0.0011 | 0.001 |
Nuclear Periphery | 0.5237 | 0.5372 | 0.4411 | 0.4687 | 0.3979 | 0.4647 | 0.6482 | 0.683 | 0.7293 | 0.8007 | 0.5273 | 0.6472 | 0.0354 | 0.7417 | 0.644 | 0.681 | 0.5297 | 0.5331 |
Nucleolus | 0.0084 | 0.0016 | 0.0005 | 0.0003 | 0.0022 | 0.0006 | 0.0017 | 0.0006 | 0.0002 | 0.0001 | 0.0026 | 0.0022 | 0.1482 | 0.0002 | 0.0003 | 0.0006 | 0.0011 | 0.0011 |
Nucleus | 0.2392 | 0.3853 | 0.2897 | 0.4356 | 0.2394 | 0.4885 | 0.1653 | 0.2385 | 0.1609 | 0.0904 | 0.1876 | 0.28 | 0.0239 | 0.1751 | 0.2306 | 0.1829 | 0.3671 | 0.3679 |
Peroxisomes | 0.0018 | 0.0002 | 0.0085 | 0.0042 | 0.013 | 0.0025 | 0.0035 | 0.0005 | 0.0155 | 0 | 0.0037 | 0.0004 | 0.0188 | 0 | 0 | 0.0003 | 0 | 0 |
Punctate Nuclear | 0.0123 | 0.0027 | 0.0034 | 0.0017 | 0.0267 | 0.0014 | 0.0062 | 0.0039 | 0.0019 | 0.0013 | 0.0204 | 0.0026 | 0.1939 | 0.0028 | 0.0041 | 0.0104 | 0.0023 | 0.0041 |
Vacuole | 0.0329 | 0.0045 | 0.0058 | 0.0007 | 0.0215 | 0.0032 | 0.0081 | 0.0062 | 0.0017 | 0.0013 | 0.0051 | 0.0024 | 0.0482 | 0.0061 | 0.0021 | 0.0064 | 0.0154 | 0.0034 |
Vacuole Periphery | 0.0118 | 0.0037 | 0.0079 | 0.0044 | 0.0102 | 0.0039 | 0.0104 | 0.0074 | 0.0087 | 0.0095 | 0.0067 | 0.0051 | 0.0057 | 0.0071 | 0.0067 | 0.0064 | 0.0065 | 0.0076 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 54.8983 | 37.6804 | 29.3134 | 36.6016 | 35.4788 | 26.1599 | 32.7986 | 25.403 | 24.5718 | 41.8372 |
Translational Efficiency | 0.5809 | 0.6476 | 0.7325 | 0.5455 | 0.608 | 0.929 | 0.5271 | 0.5808 | 0.5693 | 0.5259 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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