Standard name
Human Ortholog
Description Beta regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.08 0.08 0.08 0.09 0.05 0.08 0.08 0.06 0.06 0.05 0.06 0.1 0.19 0.06 0.07 0.1 0 0 0 0.18 0.17 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0.05 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.62 0.76 0.78 0.78 0.73 0.68 0.63 0.63 0.62 0.59 0.58 0.83 0.78 0.62 0.81 0.61 0.63 0.75 0.77 0.66 0.5 0.36 0.32
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.12 0.06 0 0.06 0.05 0.08 0.07 0.08 0.08 0 0 0.05 0 0.05 0.06 0.07 0.05 0.09 0.05 0.07 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.4 0.17 0.07 0.12 0.15 0.23 0.28 0.18 0.22 0.25 0.25 0.13 0.12 0.15 0.1 0.31 0.27 0.1 0.1 0.12 0.12 0.23 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 2 1 0 1 0 0 1 0 0 2 0 0 1 0 0 0 0 0 1
Bud 0 0 0 1 0 0 2 0 3 1 6 5 0 0 0 1 0 0 6 3 12 8 13 6
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 2 0 1 0
Bud Site 0 0 0 0 1 1 3 3 4 5 7 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 2 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 8 32 34 39 58 41 56 39 34 31 30 12 20 54 7 13 16 0 3 5 40 47 62
Endoplasmic Reticulum 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 4 5 3 0 0 4 2 3 3
Endosome 1 0 1 1 6 11 22 4 12 15 19 14 1 2 10 0 2 0 1 1 5 4 10 12
Golgi 3 0 7 2 1 6 3 2 0 0 0 0 0 0 0 0 1 0 0 1 2 2 1 3
Mitochondria 0 0 1 1 2 6 8 0 63 31 39 36 0 1 0 5 4 2 0 0 5 3 2 2
Nucleus 113 92 292 324 404 485 517 445 319 355 306 335 168 159 179 100 112 97 114 137 289 114 99 101
Nuclear Periphery 1 0 0 0 1 0 2 0 0 4 0 2 0 0 0 0 0 0 1 0 5 1 2 3
Nucleolus 2 2 2 48 30 24 46 37 43 41 42 46 4 7 14 3 10 9 10 9 38 12 19 21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 2 5 0 2 3 0 0 0 0 0 1 0 1 0 4 5 5 7
Vac/Vac Membrane 21 59 66 29 61 97 174 194 91 126 130 146 26 24 44 12 57 42 14 18 53 27 61 78
Unique Cell Count 135 149 382 414 519 660 757 703 509 572 520 574 203 204 289 124 184 155 154 179 436 229 274 314
Labelled Cell Count 144 161 402 441 547 691 823 747 576 615 583 622 211 214 303 132 205 170 154 179 436 229 274 314


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 13.3 13.5 13.0 12.6 11.1 10.4 10.6 8.6 9.7 9.3 9.5 13.9 13.2 12.7 12.2 15.2 17.5 12.3 12.9 12.2
Std Deviation (1e-4) 2.6 2.6 3.1 4.0 4.1 3.3 2.5 2.4 2.6 2.6 2.6 2.4 3.5 3.5 3.7 2.9 4.3 4.8 3.7 4.6 3.8
Intensity Change (Log2) – – – -0.05 -0.1 -0.29 -0.37 -0.35 -0.65 -0.47 -0.55 -0.51 0.04 -0.03 -0.09 -0.15 0.17 0.37 -0.14 -0.06 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -0.5 0.2 -1.9 -0.2 -0.4 -1.5 -1.4 -1.9 -1.1 0.6 4.0 -1.0 -0.5 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 3.0 0 2.6 2.8 3.4 2.7 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 6.9 4.3 5.2 4.7 0 0 0 0 0 0
Nucleus 0.6 0.5 -1.1 -2.9 -4.4 -4.4 -4.7 -5.5 -5.8 1.8 0.4 -4.1 1.0 -3.8 -3.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 6.4 4.2 3.1 4.4 4.0 5.3 4.8 5.2 5.2 0 0 3.6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.5 -2.4 -1.1 2.2 3.8 0.2 1.8 2.8 3.0 -1.4 -1.8 -0.7 -2.0 3.7 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.0539 7.3783 6.9533 6.3552 6.4246 6.4996 8.0849 9.2083 9.0736 9.4915 7.9367 8.8897 8.3051 8.9714 7.8826 7.3158 7.6848 7.795
Actin 0.0186 0 0.0116 0.0032 0.0002 0.0065 0.0247 0.0001 0.0032 0.0001 0.0001 0.0001 0.0108 0.0001 0.0009 0.0002 0.0001 0.0026
Bud 0.0009 0.0001 0.0006 0.0002 0.0001 0.0004 0.001 0.0001 0.0003 0.0002 0.0001 0.0002 0.0002 0.0001 0.0004 0.0001 0 0.0002
Bud Neck 0.0039 0.0007 0.0013 0.0011 0.0003 0.0338 0.0021 0.0022 0.0018 0.0032 0.0009 0.0145 0.001 0.0006 0.0025 0.0009 0.0008 0.0112
Bud Periphery 0.0022 0.0001 0.0014 0.0004 0 0.0007 0.0023 0.0002 0.0005 0.0004 0.0002 0.0004 0.0003 0.0001 0.0013 0.0001 0.0001 0.0004
Bud Site 0.005 0.0007 0.0032 0.0006 0.0001 0.0018 0.002 0.0015 0.0027 0.0009 0.0002 0.0006 0.0005 0.0014 0.0019 0.0006 0.0001 0.0004
Cell Periphery 0.0014 0.0001 0.0005 0.0001 0 0.0003 0.0005 0.0002 0.0005 0.0002 0 0.0003 0.0001 0.0001 0.0006 0 0 0.0002
Cytoplasm 0.0103 0.0011 0.0025 0.001 0.0031 0.0039 0.0095 0.0008 0.0119 0.0007 0.0017 0.0009 0.0054 0.0041 0.003 0.0012 0.0007 0.0009
Cytoplasmic Foci 0.0354 0 0.0039 0.0007 0 0.0038 0.0093 0 0.0115 0 0 0 0.0112 0.0035 0.001 0.0147 0 0.0027
Eisosomes 0.0003 0 0.0003 0.0004 0 0 0.0003 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0035 0.0001 0.0008 0.0003 0.0004 0.0036 0.0108 0.0001 0.0093 0.0001 0.0004 0.0001 0.0024 0.0004 0.0022 0.0002 0.0002 0.0018
Endosome 0.0136 0.0001 0.0043 0.0014 0.0008 0.0074 0.0561 0.0002 0.003 0.0001 0.0003 0.0002 0.0114 0.0033 0.0046 0.0088 0.0003 0.0107
Golgi 0.0058 0 0.0032 0.0008 0 0.0012 0.0129 0 0.0017 0 0 0 0.0038 0.0006 0.001 0.0014 0 0.0033
Lipid Particles 0.0098 0 0.0017 0.0123 0 0.0027 0.0106 0 0.0013 0 0 0 0.0124 0.0008 0.0025 0.0038 0 0.0045
Mitochondria 0.0126 0.0002 0.0098 0.0336 0.0007 0.0007 0.0091 0.0003 0.0008 0.0003 0.0004 0.0002 0.0033 0.0007 0.0088 0.0004 0.0006 0.0052
None 0.0103 0 0.0006 0.0013 0.0003 0.0007 0.0053 0.0001 0.0021 0 0 0.0002 0.0011 0.0009 0.0002 0.0005 0.0001 0.0001
Nuclear Periphery 0.0171 0.0027 0.0064 0.0068 0.0194 0.003 0.0495 0.0051 0.006 0.0033 0.0138 0.0036 0.0156 0.0072 0.0053 0.0051 0.0049 0.0023
Nucleolus 0.0184 0.0261 0.0353 0.0321 0.0067 0.0328 0.0403 0.0462 0.0746 0.0372 0.0078 0.0443 0.0081 0.0241 0.0396 0.017 0.0118 0.0342
Nucleus 0.7997 0.9659 0.8948 0.8962 0.9659 0.8907 0.7042 0.9399 0.8559 0.9512 0.9705 0.93 0.8992 0.9473 0.9067 0.932 0.9785 0.9149
Peroxisomes 0.0059 0 0.0099 0.0044 0 0.0008 0.0032 0 0.0057 0 0 0 0.0022 0.0004 0.0014 0.0051 0 0.0008
Punctate Nuclear 0.0122 0.0015 0.0027 0.0021 0.0005 0.0021 0.0328 0.0015 0.0017 0.0009 0.0009 0.0026 0.0091 0.0027 0.001 0.0074 0.001 0.0016
Vacuole 0.0102 0.0005 0.0044 0.0006 0.0006 0.0028 0.0085 0.0013 0.0051 0.001 0.0019 0.0017 0.0013 0.0015 0.0134 0.0004 0.0005 0.0014
Vacuole Periphery 0.0029 0.0001 0.001 0.0005 0.0008 0.0003 0.0049 0.0002 0.0004 0.0002 0.0006 0.0001 0.0006 0.0002 0.0017 0.0001 0.0002 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.3702 56.5664 64.711 62.3567 68.3686 80.3709 64.0456 65.1413 83.3289 66.0917
Translational Efficiency 1.2771 1.7813 1.4164 1.8469 1.395 1.2906 1.4974 1.3122 1.0321 1.2037

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Beta regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Ckb1

Ckb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ckb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available