Standard name | |
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Human Ortholog | |
Description | Protein kinase; along with its paralog, ALK2, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of cell polarity factors in mitosis, proper localization of formins and polarity factors, and survival in cells that activate spindle assembly checkpoint; phosphorylated in response to DNA damage; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 6 | 11 | 24 | 2 | 1 | 4 |
Bud | 0 | 4 | 4 | 2 | 2 | 4 | 2 | 11 | 12 | 12 | 16 | 13 | 0 | 0 | 0 | – | 1 | 0 | 4 | 10 | 3 | 1 | 3 | 4 |
Bud Neck | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | – | 1 | 0 | 5 | 13 | 14 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 16 | 21 | 6 | 8 | 4 | 6 | 7 | 7 | 6 | 9 | 13 | 0 | 0 | 5 | – | 17 | 30 | 6 | 11 | 25 | 1 | 0 | 0 |
Cytoplasm | 152 | 315 | 263 | 174 | 265 | 353 | 284 | 361 | 210 | 278 | 235 | 222 | 121 | 121 | 188 | – | 225 | 249 | 130 | 251 | 156 | 77 | 176 | 192 |
Endoplasmic Reticulum | 3 | 3 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 3 | 0 | 2 | 1 | 1 | 0 | – | 19 | 10 | 1 | 2 | 13 | 1 | 2 | 0 |
Endosome | 0 | 1 | 3 | 0 | 5 | 2 | 5 | 5 | 2 | 3 | 2 | 3 | 0 | 0 | 0 | – | 3 | 2 | 0 | 5 | 3 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3 | 1 | 0 | 0 | 0 | 3 | 0 |
Mitochondria | 2 | 12 | 11 | 17 | 17 | 173 | 124 | 231 | 190 | 359 | 259 | 265 | 0 | 0 | 0 | – | 8 | 8 | 9 | 11 | 24 | 5 | 6 | 10 |
Nucleus | 1 | 1 | 0 | 4 | 0 | 0 | 0 | 4 | 0 | 3 | 1 | 2 | 2 | 2 | 1 | – | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 9 | 7 | 9 | 12 | 12 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 3 |
Vac/Vac Membrane | 1 | 0 | 3 | 3 | 6 | 6 | 8 | 7 | 4 | 11 | 8 | 8 | 0 | 0 | 0 | – | 5 | 0 | 3 | 6 | 4 | 1 | 3 | 4 |
Unique Cell Count | 156 | 329 | 275 | 190 | 282 | 423 | 353 | 510 | 344 | 519 | 418 | 433 | 123 | 123 | 189 | 242 | 263 | 176 | 333 | 279 | 95 | 204 | 229 | |
Labelled Cell Count | 168 | 354 | 309 | 213 | 308 | 551 | 434 | 642 | 438 | 691 | 545 | 543 | 125 | 125 | 195 | 282 | 307 | 176 | 333 | 279 | 95 | 204 | 229 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 4.6 | 4.5 | 4.2 | 4.3 | 3.3 | 3.5 | 3.6 | 3.1 | 2.9 | 2.9 | 3.2 | 5.2 | 5.2 | 5.2 | 4.8 | 5.0 | 6.2 | 4.5 | 5.3 | 5.5 |
Std Deviation (1e-4) | 1.7 | 1.4 | 1.7 | 2.1 | 2.1 | 1.5 | 1.9 | 1.7 | 1.0 | 0.9 | 0.9 | 1.5 | 1.0 | 1.0 | 1.1 | 1.5 | 1.5 | 2.2 | 1.1 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.06 | -0.43 | -0.34 | -0.31 | -0.53 | -0.62 | -0.64 | -0.49 | 0.22 | 0.22 | 0.22 | 0.12 | 0.17 | 0.47 | 0.02 | 0.26 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7715 | 0.4946 | 0.9188 | 1.2918 | 2.0554 | 1.065 | 1.9236 | 1.4808 | 1.9391 | 2.7228 | 2.6487 | 2.6616 | 1.1013 | 2.2777 | 2.1569 | 2.8336 | 3.2742 | 2.2724 |
Actin | 0.0581 | 0.0039 | 0.0089 | 0.0005 | 0.0272 | 0.016 | 0.1581 | 0.001 | 0.0061 | 0.0005 | 0.0289 | 0.0112 | 0.0004 | 0.0003 | 0.001 | 0.0025 | 0.002 | 0.0095 |
Bud | 0.0023 | 0.0118 | 0.0021 | 0.0002 | 0.0143 | 0.0039 | 0.0025 | 0.0002 | 0.0031 | 0.0001 | 0.0129 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0029 | 0.0001 | 0.0007 |
Bud Neck | 0.0039 | 0.0002 | 0.0006 | 0.0015 | 0.0018 | 0.0078 | 0.0025 | 0.001 | 0.0013 | 0.0005 | 0.0032 | 0.0015 | 0.0006 | 0.0002 | 0.0005 | 0.0025 | 0.0019 | 0.0029 |
Bud Periphery | 0.0079 | 0.0051 | 0.0059 | 0.001 | 0.024 | 0.009 | 0.0041 | 0.0007 | 0.011 | 0.0003 | 0.0323 | 0.0021 | 0.0007 | 0.0002 | 0.0003 | 0.0051 | 0.0008 | 0.0016 |
Bud Site | 0.0131 | 0.0203 | 0.008 | 0.0007 | 0.0057 | 0.0014 | 0.0095 | 0.0086 | 0.0045 | 0.0002 | 0.0025 | 0.0003 | 0.0009 | 0.0022 | 0.0007 | 0.0095 | 0.0005 | 0.001 |
Cell Periphery | 0.0012 | 0.0015 | 0.0016 | 0.0048 | 0.0038 | 0.001 | 0.0005 | 0.001 | 0.0012 | 0.0023 | 0.004 | 0.0012 | 0.0035 | 0.0036 | 0.0032 | 0.0042 | 0.0143 | 0.0017 |
Cytoplasm | 0.122 | 0.2632 | 0.2854 | 0.6301 | 0.5795 | 0.4073 | 0.1148 | 0.1966 | 0.2535 | 0.6674 | 0.6918 | 0.3974 | 0.2368 | 0.2361 | 0.4227 | 0.771 | 0.881 | 0.4592 |
Cytoplasmic Foci | 0.016 | 0.0036 | 0.0159 | 0.0034 | 0.0177 | 0.0101 | 0.0236 | 0.0025 | 0.0086 | 0.0019 | 0.0154 | 0.0046 | 0.002 | 0.0017 | 0.002 | 0.0029 | 0.0023 | 0.002 |
Eisosomes | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0007 | 0.0001 |
Endoplasmic Reticulum | 0.0045 | 0.0016 | 0.0031 | 0.0026 | 0.004 | 0.0022 | 0.0023 | 0.0008 | 0.0034 | 0.0032 | 0.0054 | 0.0013 | 0.0016 | 0.0012 | 0.0026 | 0.0031 | 0.0024 | 0.0015 |
Endosome | 0.0194 | 0.0009 | 0.0116 | 0.0008 | 0.0536 | 0.0065 | 0.02 | 0.0005 | 0.0012 | 0.0004 | 0.0026 | 0.0071 | 0.0003 | 0.0002 | 0.0003 | 0.0005 | 0.0008 | 0.0002 |
Golgi | 0.0109 | 0.0008 | 0.006 | 0.0002 | 0.0263 | 0.0019 | 0.0228 | 0.0001 | 0.0008 | 0.0001 | 0.0061 | 0.0032 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 |
Lipid Particles | 0.0106 | 0.0002 | 0.0066 | 0.0002 | 0.0171 | 0.0026 | 0.018 | 0.0001 | 0.0025 | 0.0002 | 0.0159 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0001 |
Mitochondria | 0.0119 | 0.0002 | 0.0092 | 0.0001 | 0.0486 | 0.0016 | 0.0122 | 0.0001 | 0.0005 | 0.0001 | 0.0008 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
None | 0.6912 | 0.6833 | 0.6224 | 0.3519 | 0.1254 | 0.5118 | 0.5649 | 0.7854 | 0.6964 | 0.3211 | 0.1333 | 0.5616 | 0.7512 | 0.7522 | 0.5644 | 0.1897 | 0.0916 | 0.518 |
Nuclear Periphery | 0.0113 | 0.0003 | 0.0009 | 0.0002 | 0.0074 | 0.0045 | 0.0011 | 0.0002 | 0.0003 | 0.0002 | 0.001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0002 |
Nucleolus | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0034 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 |
Nucleus | 0.0025 | 0.0011 | 0.0007 | 0.0004 | 0.0126 | 0.003 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0014 | 0.0003 | 0.0003 | 0.0004 | 0.0004 | 0.0015 | 0.0002 | 0.0003 |
Peroxisomes | 0.005 | 0.0001 | 0.0057 | 0 | 0.0039 | 0.0043 | 0.0385 | 0.0001 | 0.0035 | 0 | 0.0394 | 0.0051 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0034 | 0.0007 | 0.0014 | 0.0001 | 0.0134 | 0.0034 | 0.0014 | 0.0002 | 0.0005 | 0.0001 | 0.0009 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0002 |
Vacuole | 0.0023 | 0.0009 | 0.0026 | 0.001 | 0.0071 | 0.0008 | 0.0011 | 0.0005 | 0.0007 | 0.0008 | 0.0018 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0016 | 0.0004 | 0.0002 |
Vacuole Periphery | 0.0012 | 0.0001 | 0.0007 | 0 | 0.0031 | 0.0003 | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.2497 | 38.0206 | 28.5336 | 25.6838 | 11.2795 | 4.3474 | 53.7323 | 52.3346 | 30.3194 | 14.7748 |
Translational Efficiency | 0.7772 | 1.1132 | 1.2079 | 0.647 | 0.5397 | 1.0179 | 0.9161 | 0.7699 | 0.8996 | 0.7604 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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