Standard name
Human Ortholog
Description Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0.09 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.14 0.27 0.29 0.3 0.37 0.31 – 0.45 0.24 0.61 – 0.18 0.29 0.47 0.17 0.16 0.19 0 0 0.05 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0.11 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.1 0.12 0.18 0.1 0.1 – 0.07 0.06 0.09 – 0.06 0.08 0.07 0 0.05 0 0.09 0.08 0.08 0.08 0.13 0.1
Golgi 0 0 0 0.05 0 0 0 – 0 0 0 – 0 0 0 0.08 0.1 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0 0 0.09 0.05 – 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0
Nuclear Periphery 0.06 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.79 0.86 0.76 0.83 0.72 0.52 0.6 – 0.37 0.66 0.43 – 0.91 0.73 0.63 0.76 0.78 0.82 0.79 0.83 0.7 0.8 0.74 0.81
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 – 0 1 0 1 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 – 1 2 2 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 28 18 12 34 34 33 – 33 12 14 – 6 58 69 41 42 44 1 2 4 0 3 4
Endoplasmic Reticulum 2 1 0 0 0 0 3 – 8 0 0 – 1 11 3 8 7 8 0 0 0 0 0 1
Endosome 1 8 7 5 20 9 11 – 5 3 2 – 2 17 11 5 12 9 8 17 7 7 26 23
Golgi 0 0 1 2 0 0 2 – 2 2 0 – 0 2 1 20 25 8 0 2 0 0 1 1
Mitochondria 8 1 0 0 1 8 5 – 4 1 1 – 1 0 0 5 8 6 2 3 2 2 5 3
Nucleus 3 6 0 0 2 0 2 – 0 0 0 – 1 5 1 6 4 2 1 3 1 5 7 4
Nuclear Periphery 4 1 0 0 0 0 0 – 0 0 0 – 1 1 1 2 6 4 3 1 4 0 5 3
Nucleolus 2 2 1 0 0 3 1 – 1 0 0 – 0 0 0 4 4 2 0 1 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 – 0 0 0 – 0 0 0 1 1 0 0 0 1 1 1 0
Vac/Vac Membrane 57 176 51 35 82 47 63 – 27 33 10 – 31 146 93 182 203 188 71 178 65 81 155 196
Unique Cell Count 72 204 67 42 114 91 105 73 50 23 34 201 148 238 260 229 91 214 94 102 210 243
Labelled Cell Count 84 223 79 54 140 101 120 81 53 29 43 241 179 275 312 271 91 214 94 102 210 243


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 9.3 9.4 10.7 9.5 7.6 8.5 – 8.4 9.1 12.0 – 8.2 8.2 7.9 13.9 13.8 13.4 7.2 7.8 8.3
Std Deviation (1e-4) 1.2 1.7 2.8 4.6 2.6 1.7 2.0 – 2.2 2.3 7.1 – 1.1 1.1 1.0 3.3 2.9 2.9 1.2 1.0 1.1
Intensity Change (Log2) – – – 0.19 0.01 -0.31 -0.15 – -0.17 -0.04 0.35 – -0.21 -0.2 -0.26 0.56 0.55 0.51 -0.38 -0.28 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.2 0.4 1.4 0.6 – 2.3 -0.4 2.9 – -1.0 0.3 2.7 -1.8 -2.0 -1.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Endosome 0 1.3 -0.1 0 – -0.8 0 0 – 0 -0.5 -0.7 0 0 0
Golgi 0 0 0 0 – 0 0 0 – 0 0 0 0 2.2 0
Mitochondria 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 – 0 0 0 0 0 0
Vacuole 0.9 -0.6 -3.1 -2.2 – -4.7 -1.2 -2.9 – 1.8 -0.6 -1.9 0.1 0.3 1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7843 2.0123 1.707 1.2869 1.5422 1.5155 1.5351 2.8367 2.3516 1.8306 1.519 2.2107 2.0425 2.6207 2.3192 2.8334 2.2123 2.5117
Actin 0.0033 0 0.0086 0 0 0 0.0057 0 0.0002 0.0002 0.0006 0.0017 0.0413 0 0.0079 0 0.0066 0.0054
Bud 0.0004 0.0002 0.0004 0.0001 0.0001 0.0001 0.0004 0.0001 0.0008 0.0003 0.0003 0.0006 0.0007 0.0001 0.0005 0 0.0007 0.0005
Bud Neck 0.0039 0.0003 0.0005 0.0001 0.0001 0.0001 0.0012 0.0002 0.0005 0.0004 0.0005 0.0002 0.0146 0.0002 0.0035 0.0001 0.0011 0.0005
Bud Periphery 0.0007 0.0002 0.0005 0.0001 0.0001 0.0002 0.0007 0.0002 0.0026 0.0006 0.0005 0.0034 0.001 0.0002 0.0008 0 0.001 0.0009
Bud Site 0.0077 0.0004 0.0009 0.0001 0.0001 0.0001 0.0022 0.0003 0.0013 0.0005 0.0013 0.0002 0.0086 0.0003 0.0052 0.0001 0.0026 0.001
Cell Periphery 0.0014 0.0004 0.0005 0.0001 0.0001 0.0002 0.0005 0.0003 0.0007 0.0005 0.0003 0.0004 0.0006 0.0003 0.0004 0.0001 0.0002 0.0002
Cytoplasm 0.0088 0.0196 0.0096 0.0007 0.0022 0.0013 0.0217 0.0075 0.0103 0.003 0.0016 0.0072 0.0114 0.0045 0.012 0.002 0.0048 0.0056
Cytoplasmic Foci 0.0305 0.01 0.0148 0.0195 0.0066 0.0028 0.0215 0.0152 0.012 0.0023 0.0166 0.0051 0.0194 0.0047 0.018 0.003 0.0193 0.0057
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0004 0 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0044 0.0044 0.0093 0.0006 0.0013 0.0016 0.0053 0.0034 0.0042 0.0015 0.0037 0.0038 0.007 0.0032 0.0042 0.0003 0.0013 0.0035
Endosome 0.3341 0.2774 0.2835 0.3326 0.3722 0.2072 0.3041 0.2296 0.2912 0.1855 0.2483 0.2258 0.2633 0.1816 0.3146 0.2198 0.2753 0.2113
Golgi 0.013 0.0047 0.0261 0.0114 0.0135 0.0078 0.0102 0.0048 0.0139 0.0043 0.0173 0.0054 0.0146 0.0037 0.0189 0.0012 0.0212 0.0081
Lipid Particles 0.0168 0.0041 0.0143 0.0198 0.0082 0.0103 0.0212 0.0038 0.0123 0.0045 0.078 0.0041 0.0165 0.0054 0.0084 0.0006 0.0142 0.0041
Mitochondria 0.0127 0.0106 0.0506 0.0012 0.0111 0.0205 0.0145 0.0042 0.0341 0.0301 0.02 0.0071 0.0094 0.0014 0.0139 0.0001 0.008 0.0086
None 0.008 0.0129 0.0012 0.0001 0.0001 0.0001 0.0119 0.0003 0.005 0.0001 0.0002 0.0017 0.004 0.0003 0.0116 0 0.0011 0.0008
Nuclear Periphery 0.0146 0.0118 0.0205 0.0017 0.0067 0.0118 0.0171 0.0091 0.0169 0.0034 0.0112 0.0381 0.0305 0.0151 0.0151 0.0015 0.0059 0.0342
Nucleolus 0.0036 0.0008 0.0011 0.0002 0.0001 0.0003 0.0027 0.0005 0.0019 0.0005 0.0011 0.0014 0.0026 0.0004 0.0018 0.0004 0.0022 0.0006
Nucleus 0.0041 0.0024 0.0127 0.0003 0.0004 0.0013 0.0054 0.0016 0.0017 0.001 0.0009 0.0023 0.0096 0.0014 0.0045 0.0006 0.0033 0.0035
Peroxisomes 0.0091 0.0002 0.0098 0.0034 0.0002 0.0003 0.0078 0.0039 0.003 0.0005 0.0293 0.0048 0.0088 0.0003 0.0023 0.0001 0.0074 0.0008
Punctate Nuclear 0.0069 0.0004 0.0004 0.0001 0 0 0.0058 0.0001 0.0003 0 0.0003 0.0001 0.0046 0.0001 0.0043 0 0.0381 0.0058
Vacuole 0.357 0.402 0.3159 0.3366 0.2066 0.3351 0.3442 0.42 0.3807 0.4239 0.2936 0.295 0.3258 0.4491 0.3303 0.5595 0.4016 0.3298
Vacuole Periphery 0.1589 0.2373 0.2187 0.2712 0.3704 0.399 0.1957 0.2947 0.2062 0.3367 0.2744 0.3916 0.205 0.3278 0.2216 0.2103 0.184 0.3688

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.0237 19.6448 25.3185 28.03 36.74 20.2294 26.266 28.5761 34.5289 34.312
Translational Efficiency 1.2482 1.3822 0.8447 0.8331 0.7792 1.837 0.9231 1.013 0.8013 0.8178

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27
Localization
Cell Percentages vacuolar membrane (63%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Pib2

Pib2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pib2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available