Standard name
Human Ortholog
Description Tryptophan synthase; catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0.09 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.98 0.93 0.92 0.86 0.88 0.85 0.78 0.79 0.98 0.99 1.0 1.0 0.99 0.99 0.97 0.95 0.98 0.91 0.91 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.12 0.09 0.1 0.1 0.12 0 0 0 0 0.05 0.05 0 0 0 0.06 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 6 8 7 8 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 2 0 0 2 0 0 3 1 5 3 1 1 0 0 0 0 0 0 1 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 11 14 7 7 22 23 33 33 56 39 56 0 1 0 0 1 3 0 0 0 0 0 0
Cytoplasm 135 315 503 413 403 506 613 645 491 542 436 436 197 194 201 108 110 82 129 309 487 63 157 138
Endoplasmic Reticulum 0 0 3 4 10 23 48 88 49 61 57 64 2 2 0 3 5 4 0 0 2 4 2 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 2 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 0 0 0 2 1 4 2 1 0 0 0 0 0 0 0 0 0 1 0
Nucleus 0 0 0 1 0 1 0 1 0 1 4 4 0 0 0 0 0 0 0 4 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 1 0 3 0 4 6 6 35 21 0 0 0 0 0 0 0 4 0 0 1 2
Unique Cell Count 136 319 507 417 412 544 664 751 560 641 560 553 200 196 201 108 111 83 134 325 495 70 172 156
Labelled Cell Count 136 329 521 426 422 555 685 776 590 681 586 594 201 197 201 111 116 89 134 325 495 70 172 156


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 103.4 111.4 76.6 70.4 63.6 57.0 51.8 49.2 43.8 38.8 33.5 38.2 82.7 90.4 86.2 158.8 155.2 141.3 75.9 80.7 79.1
Std Deviation (1e-4) 27.6 25.0 13.9 16.0 15.1 12.2 11.5 11.4 10.2 8.7 8.4 9.5 18.4 17.4 18.4 51.0 42.8 48.8 17.9 21.7 30.4
Intensity Change (Log2) – – – -0.12 -0.27 -0.43 -0.56 -0.64 -0.81 -0.98 -1.19 -1.0 0.11 0.24 0.17 1.05 1.02 0.88 -0.01 0.08 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.1 -1.1 1.1 0.7 1.5 2.5 4.2 3.2 4.8 0 0 0 0 0 0
Cytoplasm -0.3 -1.8 -5.1 -5.5 -8.2 -7.5 -8.6 -10.7 -10.4 -0.9 -0.3 1.3 0.9 -0.1 -0.4
Endoplasmic Reticulum 0 2.3 3.8 5.5 7.5 6.2 6.5 6.8 7.3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 5.5 4.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 53.9847 76.4937 63.6777 59.0629 88.4524 77.7477 111.11 133.1189 121.6713 113.7497 118.4589 135.0905 92.9854 129.1728 118.4861 106.2905 99.6318 119.1814
Actin 0.0021 0.0001 0.0023 0.0013 0.0002 0.0001 0.0001 0 0.0001 0 0 0.0001 0.0106 0.0001 0.0002 0.0002 0.0021 0.0002
Bud 0.0017 0.0005 0.0005 0.0007 0.0002 0.0002 0.0001 0 0 0.0001 0 0 0.0007 0.0005 0 0.0006 0.001 0.0001
Bud Neck 0.0009 0.0008 0.0008 0.0012 0.0012 0.0022 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0005 0.0001 0.0001 0.0001 0.0003 0.0004
Bud Periphery 0.0011 0.0005 0.0006 0.0005 0.0001 0.0002 0.0002 0 0 0.0001 0 0 0.0005 0.0001 0 0.0002 0.0009 0
Bud Site 0.0014 0.0005 0.0003 0.0136 0.0001 0.0002 0 0 0 0 0 0 0.0008 0.0001 0 0 0.0002 0
Cell Periphery 0.0022 0.0026 0.0021 0.0005 0.0009 0.0011 0.0001 0.0001 0.0002 0 0 0 0.0011 0 0.0001 0 0.0007 0.0001
Cytoplasm 0.5254 0.6093 0.5371 0.5021 0.8211 0.683 0.9878 0.9988 0.9938 0.9932 0.9974 0.9971 0.8896 0.9894 0.9937 0.9752 0.8989 0.9917
Cytoplasmic Foci 0.0127 0.0004 0.0064 0.0029 0.0132 0.0199 0.0002 0.0001 0.0005 0.0003 0.0001 0.0001 0.0141 0.0059 0.0005 0.001 0.0108 0.0006
Eisosomes 0.0001 0 0.0001 0 0.0002 0 0 0 0.0001 0 0 0 0.0002 0 0.0001 0 0.0009 0
Endoplasmic Reticulum 0.0015 0.0002 0.0003 0.0017 0.0003 0.0047 0.0004 0.0001 0.0002 0.0001 0 0.0002 0.0043 0.0001 0.0003 0.0004 0.0004 0.0003
Endosome 0.0051 0.0003 0.0013 0.0034 0.0007 0.0011 0 0 0.0001 0.0001 0 0 0.0072 0 0 0.0005 0.0009 0.0002
Golgi 0.0015 0 0.002 0.0011 0.0001 0.0001 0 0 0 0 0 0 0.0021 0 0 0 0.0003 0
Lipid Particles 0.0063 0.0019 0.0064 0.0006 0.0041 0.0055 0 0 0.0005 0 0 0 0.0058 0.0004 0.0002 0 0.0061 0
Mitochondria 0.0042 0.0019 0.0068 0.0007 0.0045 0.0013 0.0002 0 0.0005 0.0001 0 0 0.0055 0.001 0.0002 0.002 0.0089 0.0001
None 0.0068 0.0001 0.0028 0.0004 0.0096 0.001 0.0006 0 0.0011 0.0019 0 0.0001 0.0063 0.0002 0.0019 0.0018 0.0395 0.0002
Nuclear Periphery 0.0094 0.0014 0.0026 0.0021 0.0036 0.0035 0.0039 0.0002 0.0004 0.0006 0.0004 0.0006 0.0052 0.0001 0.0008 0.0031 0.0016 0.0012
Nucleolus 0.334 0.2957 0.3459 0.3858 0.1045 0.2184 0.0017 0 0.0004 0 0 0 0.0079 0.0005 0.0001 0.0005 0.0043 0.0001
Nucleus 0.0476 0.0721 0.0639 0.0778 0.0203 0.0325 0.0037 0.0005 0.001 0.0021 0.0015 0.0013 0.0135 0.0011 0.0012 0.0097 0.0035 0.003
Peroxisomes 0.0026 0.0002 0.0021 0.0002 0.0003 0.0002 0 0 0 0 0 0 0.0012 0.0001 0 0.0001 0.0014 0
Punctate Nuclear 0.0019 0.0002 0.0004 0.0005 0.0004 0.0014 0.0001 0 0.0001 0 0 0 0.0053 0 0.0002 0.0004 0.0027 0.0001
Vacuole 0.0224 0.0078 0.0082 0.0022 0.0076 0.0169 0.0004 0.0001 0.0005 0.0009 0.0002 0.0001 0.0101 0.0002 0.0002 0.0021 0.0099 0.0012
Vacuole Periphery 0.0091 0.0036 0.0071 0.0006 0.0069 0.0066 0.0002 0 0.0002 0.0002 0 0 0.0075 0.0001 0.0001 0.0019 0.0046 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 295.3321 287.9252 286.0193 256.7422 253.5901 263.2375 280.556 255.75 270.721 252.4641
Translational Efficiency 1.8128 1.8883 1.7482 2.037 2.0756 2.0377 1.8049 1.9331 1.8032 1.7892

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Tryptophan synthase; catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Trp5

Trp5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trp5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available