Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 0 | 7 | 0 | 2 | 0 | – | – | – | 2 | 1 | 0 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 2 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 64 | 67 | 36 | 8 | 159 | 124 | 228 | 172 | 102 | 89 | 73 | 63 | 51 | 133 | 178 | – | – | – | 70 | 92 | 60 | 104 | 133 | 192 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 3 | 0 | 4 | – | – | – | 0 | 0 | 1 | 1 | 2 | 0 |
Endosome | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | – | – | – | 0 | 0 | 0 | 1 | 1 | 2 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 9 | 0 | 2 | 2 | 5 | 8 | 16 | 39 | 27 | 47 | 32 | 0 | 0 | 0 | – | – | – | 6 | 1 | 0 | 0 | 0 | 0 |
Nucleus | 1 | 4 | 0 | 2 | 3 | 3 | 7 | 6 | 8 | 4 | 10 | 7 | 4 | 2 | 5 | – | – | – | 3 | 18 | 6 | 19 | 11 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 2 |
Vac/Vac Membrane | 1 | 6 | 6 | 14 | 32 | 4 | 15 | 15 | 3 | 6 | 2 | 8 | 3 | 24 | 19 | – | – | – | 3 | 9 | 1 | 5 | 8 | 12 |
Unique Cell Count | 65 | 77 | 42 | 24 | 185 | 135 | 244 | 201 | 142 | 114 | 112 | 102 | 61 | 148 | 201 | 93 | 130 | 74 | 138 | 167 | 222 | |||
Labelled Cell Count | 68 | 88 | 43 | 29 | 198 | 138 | 260 | 211 | 158 | 128 | 135 | 119 | 61 | 162 | 209 | 93 | 130 | 74 | 138 | 167 | 222 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 4.9 | 7.3 | 9.2 | 8.0 | 6.0 | 6.3 | 6.3 | 5.0 | 5.4 | 4.9 | 4.9 | 8.1 | 7.8 | 7.8 | – | – | – | 7.9 | 9.0 | 8.1 |
Std Deviation (1e-4) | 2.0 | 0.8 | 0.9 | 2.4 | 1.6 | 1.0 | 1.8 | 1.9 | 1.3 | 1.4 | 1.1 | 0.9 | 1.5 | 1.8 | 1.5 | – | – | – | 1.7 | 2.0 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.33 | 0.12 | -0.29 | -0.23 | -0.21 | -0.56 | -0.46 | -0.58 | -0.58 | 0.15 | 0.09 | 0.08 | – | – | – | 0.1 | 0.29 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.6619 | 4.0555 | 4.1937 | 3.6181 | 3.7442 | 3.7911 | 6.0544 | 6.6355 | 6.8024 | 6.8303 | 6.0254 | 6.7617 | 3.8948 | 4.8123 | 4.3671 | 4.8825 | 3.66 | 4.5646 |
Actin | 0.0209 | 0.0004 | 0.0205 | 0.0025 | 0.0026 | 0.0029 | 0.0013 | 0.0054 | 0.0137 | 0.0015 | 0.0014 | 0.0009 | 0.0281 | 0.0004 | 0.0196 | 0.0009 | 0.0127 | 0.0004 |
Bud | 0.0003 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0001 |
Bud Neck | 0.001 | 0.0009 | 0.001 | 0.0012 | 0.001 | 0.0052 | 0.0006 | 0.0008 | 0.0015 | 0.0019 | 0.0017 | 0.0032 | 0.0014 | 0.0007 | 0.0015 | 0.0017 | 0.0017 | 0.0032 |
Bud Periphery | 0.0005 | 0 | 0.0009 | 0 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0.0005 | 0 | 0.0003 | 0 |
Bud Site | 0.0025 | 0.0028 | 0.0047 | 0.0002 | 0.0001 | 0.0002 | 0.0009 | 0.0012 | 0.0034 | 0.0007 | 0.0003 | 0.0002 | 0.0018 | 0.0006 | 0.0081 | 0.0005 | 0.0004 | 0.0001 |
Cell Periphery | 0.0004 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.7397 | 0.7512 | 0.6549 | 0.7376 | 0.7997 | 0.7065 | 0.7509 | 0.6535 | 0.715 | 0.6816 | 0.7263 | 0.7469 | 0.7474 | 0.8026 | 0.7393 | 0.6706 | 0.7642 | 0.8097 |
Cytoplasmic Foci | 0.0085 | 0.0021 | 0.0117 | 0.0042 | 0.0035 | 0.004 | 0.0113 | 0.0046 | 0.0164 | 0.0031 | 0.0254 | 0.0124 | 0.0104 | 0.0011 | 0.0083 | 0.003 | 0.0056 | 0.0026 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0149 | 0.0093 | 0.0096 | 0.0057 | 0.0078 | 0.0055 | 0.0153 | 0.007 | 0.0159 | 0.0117 | 0.0089 | 0.0084 | 0.0172 | 0.0049 | 0.0099 | 0.0088 | 0.0095 | 0.0066 |
Endosome | 0.0095 | 0.0024 | 0.0086 | 0.0019 | 0.0137 | 0.0053 | 0.0167 | 0.0031 | 0.0131 | 0.0026 | 0.0162 | 0.0081 | 0.0052 | 0.0012 | 0.0111 | 0.0036 | 0.026 | 0.0019 |
Golgi | 0.0045 | 0.0001 | 0.0041 | 0.001 | 0.0038 | 0.0006 | 0.0012 | 0.0005 | 0.0024 | 0.0002 | 0.001 | 0.0004 | 0.0029 | 0 | 0.0027 | 0.0002 | 0.0222 | 0 |
Lipid Particles | 0.0051 | 0.0001 | 0.0029 | 0.0018 | 0.0097 | 0.0031 | 0.0019 | 0.0002 | 0.0027 | 0.0002 | 0.0005 | 0.0008 | 0.0025 | 0.0001 | 0.0011 | 0.0006 | 0.0068 | 0 |
Mitochondria | 0.0042 | 0.0002 | 0.0038 | 0.0003 | 0.005 | 0.0027 | 0.0007 | 0.0005 | 0.0012 | 0.0003 | 0.0003 | 0.0005 | 0.0053 | 0.0001 | 0.0015 | 0.0003 | 0.0138 | 0.0002 |
None | 0.0169 | 0.0208 | 0.0102 | 0.0014 | 0.008 | 0.0053 | 0.0439 | 0.0043 | 0.0275 | 0.0513 | 0.0611 | 0.037 | 0.0273 | 0.0047 | 0.0103 | 0.0438 | 0.0027 | 0.0111 |
Nuclear Periphery | 0.0214 | 0.0175 | 0.0221 | 0.01 | 0.0185 | 0.011 | 0.0167 | 0.0154 | 0.0158 | 0.0136 | 0.0123 | 0.0075 | 0.0122 | 0.0085 | 0.0133 | 0.0145 | 0.0371 | 0.0075 |
Nucleolus | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0027 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0006 | 0.0007 | 0.0001 | 0.0001 |
Nucleus | 0.1387 | 0.1874 | 0.2321 | 0.1794 | 0.1184 | 0.2376 | 0.1228 | 0.297 | 0.1531 | 0.2234 | 0.1334 | 0.1656 | 0.1279 | 0.1718 | 0.1546 | 0.2428 | 0.0784 | 0.1525 |
Peroxisomes | 0.0029 | 0.0001 | 0.0044 | 0.048 | 0.0012 | 0.0005 | 0.0017 | 0.0003 | 0.007 | 0.0001 | 0.0016 | 0.0008 | 0.0035 | 0 | 0.0026 | 0.0002 | 0.0016 | 0 |
Punctate Nuclear | 0.004 | 0.0027 | 0.0041 | 0.0031 | 0.0036 | 0.0056 | 0.006 | 0.0036 | 0.007 | 0.0051 | 0.0073 | 0.0044 | 0.0031 | 0.0024 | 0.012 | 0.006 | 0.0025 | 0.0026 |
Vacuole | 0.0026 | 0.0015 | 0.0022 | 0.0014 | 0.0015 | 0.002 | 0.0037 | 0.0017 | 0.0027 | 0.0017 | 0.0015 | 0.0018 | 0.0018 | 0.0005 | 0.0016 | 0.0014 | 0.004 | 0.0011 |
Vacuole Periphery | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0014 | 0.0003 | 0.0009 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.01 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.0962 | 7.8716 | 4.9425 | 6.6331 | 4.3911 | 8.8007 | 6.1223 | 5.9916 | 6.5787 | 7.8505 |
Translational Efficiency | 0.5532 | 0.4722 | 0.6032 | 0.593 | 0.8285 | 0.4812 | 0.7215 | 0.7492 | 0.6807 | 0.6691 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|