Standard name
Human Ortholog
Description Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0.06 0.05 0.05 0 – 0.05 0.07 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.98 0.95 0.93 0.96 0.9 0.83 0.79 – 0.67 0.68 0.6 0.59 0.95 0.96 0.95 0.89 0.88 0.93 0.95 0.95 0.87 0.74 0.8 0.84
Endoplasmic Reticulum 0 0 0 0 0.08 0.12 0.15 – 0.1 0.06 0.08 0.07 0 0.05 0 0 0 0 0 0 0 0.19 0.12 0.1
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 – 0.25 0.27 0.38 0.42 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0.12 0.1 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 4 8 – 7 11 4 5 0 2 1 0 0 1 0 0 0 0 0 0
Bud 0 3 1 0 0 0 0 – 1 2 1 0 0 1 0 0 1 0 0 3 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 31 11 5 8 8 7 – 10 16 7 4 6 15 17 7 15 6 0 0 0 1 0 0
Cytoplasm 176 699 174 75 150 125 169 – 133 152 70 68 440 479 452 268 284 247 161 725 184 229 350 398
Endoplasmic Reticulum 3 25 6 1 14 18 31 – 20 14 9 8 16 26 18 10 12 2 2 6 6 57 51 48
Endosome 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0
Golgi 0 0 1 0 0 2 1 – 3 1 0 0 0 0 0 0 2 0 0 1 1 1 0 2
Mitochondria 1 2 2 1 0 6 10 – 49 61 45 49 2 0 0 1 3 2 1 2 4 3 7 4
Nucleus 0 1 3 0 1 0 2 – 0 0 2 1 4 2 1 3 1 0 0 4 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 1 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 8 3 0 0 0 4 – 3 10 5 6 3 2 0 35 32 18 1 8 5 11 16 16
Unique Cell Count 180 737 187 78 167 150 213 198 223 117 116 462 498 474 300 324 266 171 761 212 308 435 477
Labelled Cell Count 182 769 203 82 173 163 233 226 267 144 142 471 527 489 324 350 276 171 761 212 308 435 477


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.2 15.0 13.8 12.9 14.4 12.4 12.4 – 11.0 9.8 8.5 9.1 15.4 16.9 16.5 20.1 22.3 19.5 14.1 15.2 16.7
Std Deviation (1e-4) 3.1 2.6 3.1 2.2 2.6 2.5 2.3 – 2.4 2.1 1.9 2.0 2.6 2.9 3.0 7.0 6.4 5.5 2.2 2.4 3.2
Intensity Change (Log2) – – – -0.1 0.07 -0.15 -0.15 – -0.32 -0.49 -0.69 -0.6 0.16 0.29 0.26 0.54 0.69 0.5 0.04 0.14 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 2.2 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.5 -0.2 -1.2 – -0.4 0.5 0 -0.9 0 -1.8 -1.3 -2.0 -0.6 -2.0
Cytoplasm 1.0 -1.1 -2.8 -3.9 – -6.3 -6.2 -7.1 -7.3 1.1 1.7 1.2 -1.4 -1.9 -0.1
Endoplasmic Reticulum 0 2.1 3.1 3.9 – 2.7 1.4 1.8 1.5 0.2 1.1 0.4 0.1 0.3 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.1 – 6.8 7.4 8.8 9.3 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 1.7 0 0 0 0 0 4.0 3.6 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.5193 13.4217 12.2934 10.6116 10.8462 12.5133 9.9763 14.3782 12.5263 11.4736 11.3755 12.8232 11.6348 13.8797 12.6909 12.5237 12.0966 12.5598
Actin 0.0159 0.0009 0.0143 0.0228 0.0092 0.0012 0.0166 0.001 0.017 0.0303 0.0048 0.0119 0.0038 0.003 0.0043 0.0073 0.002 0.0071
Bud 0.0008 0.0004 0.0008 0.001 0.0008 0.0006 0.0012 0.0023 0.0034 0.0212 0.0009 0.0036 0.0037 0.0138 0.0071 0.0009 0.0024 0.0079
Bud Neck 0.0008 0.0004 0.0006 0.0008 0.0014 0.0014 0.0016 0.0003 0.0013 0.0013 0.0005 0.0013 0.0009 0.0004 0.0009 0.0035 0.0013 0.0018
Bud Periphery 0.0008 0.0002 0.0004 0.0005 0.0006 0.0004 0.0014 0.0007 0.0017 0.0048 0.0007 0.0021 0.0016 0.0044 0.0033 0.0009 0.0006 0.0037
Bud Site 0.0014 0.001 0.0022 0.0008 0.0009 0.0002 0.0032 0.0063 0.0024 0.0017 0.0019 0.0004 0.0026 0.0164 0.0153 0.0017 0.0059 0.0012
Cell Periphery 0.0019 0.0005 0.0008 0.0003 0.0003 0.0004 0.0017 0.0003 0.0006 0.0004 0.0001 0.0002 0.0005 0.0005 0.0007 0.0005 0.0002 0.0004
Cytoplasm 0.7076 0.9447 0.8661 0.6852 0.7573 0.8093 0.6389 0.9221 0.8327 0.6173 0.7216 0.7953 0.8184 0.9143 0.8492 0.8139 0.8653 0.8528
Cytoplasmic Foci 0.0201 0.006 0.0139 0.0199 0.0245 0.0194 0.0401 0.0159 0.0158 0.0249 0.0207 0.0195 0.0191 0.0105 0.0181 0.0172 0.0238 0.0218
Eisosomes 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0.0004 0 0 0 0 0 0.0002 0 0
Endoplasmic Reticulum 0.1336 0.0229 0.0279 0.0796 0.025 0.0861 0.0711 0.0184 0.031 0.0232 0.0888 0.0472 0.0531 0.0068 0.0267 0.0324 0.0091 0.0347
Endosome 0.053 0.0124 0.0416 0.1035 0.11 0.0458 0.1079 0.0187 0.0368 0.1015 0.0792 0.0733 0.0435 0.0154 0.0389 0.0503 0.0485 0.0395
Golgi 0.0088 0.0019 0.0077 0.0055 0.0122 0.0068 0.0165 0.002 0.0059 0.0225 0.0117 0.0108 0.004 0.0013 0.0036 0.0054 0.0117 0.0033
Lipid Particles 0.0046 0.0001 0.0007 0.0029 0.0041 0.0001 0.0105 0.0002 0.0099 0.0174 0.0026 0.0005 0.0009 0.0001 0.0004 0.0007 0.0036 0.0004
Mitochondria 0.0071 0.0008 0.0021 0.0033 0.0089 0.0142 0.0156 0.0009 0.0066 0.0491 0.0018 0.0069 0.0036 0.0007 0.0114 0.0087 0.0032 0.0022
None 0.0035 0.0005 0.0009 0.0028 0.0086 0.003 0.0038 0.0014 0.0044 0.0045 0.0014 0.0012 0.0057 0.0026 0.0034 0.0013 0.0015 0.0026
Nuclear Periphery 0.0091 0.0013 0.0021 0.0194 0.01 0.002 0.015 0.001 0.0045 0.0052 0.0055 0.0101 0.0062 0.0008 0.0022 0.0072 0.0015 0.0029
Nucleolus 0.0001 0 0 0.0003 0.0004 0 0.0004 0.0001 0.0011 0.001 0.0006 0.0001 0.001 0.0001 0.0003 0.0002 0.0003 0.0001
Nucleus 0.0031 0.001 0.0013 0.0033 0.0028 0.0012 0.0049 0.0011 0.0023 0.0034 0.003 0.0019 0.0068 0.0018 0.0032 0.0035 0.0026 0.0031
Peroxisomes 0.0045 0 0.0004 0.0026 0.0016 0.0001 0.0088 0.0002 0.002 0.0151 0.0043 0.0008 0.0003 0.0002 0.0002 0.0003 0.0005 0.0003
Punctate Nuclear 0.0043 0.0001 0.0002 0.0103 0.0073 0.0004 0.0089 0.0005 0.0021 0.0007 0.037 0.0003 0.0013 0.0003 0.0007 0.0005 0.0014 0.0008
Vacuole 0.0154 0.004 0.014 0.0264 0.0095 0.0049 0.0263 0.0055 0.0155 0.0408 0.0096 0.0078 0.0194 0.0058 0.0082 0.0369 0.0125 0.0111
Vacuole Periphery 0.0033 0.0008 0.0021 0.0085 0.0043 0.0024 0.0055 0.0011 0.0028 0.0133 0.0032 0.0047 0.0037 0.0008 0.0019 0.0066 0.0023 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.9762 31.9809 47.1957 42.6679 32.052 40.9781 41.8731 39.9707 41.7288 36.7575
Translational Efficiency 2.1554 2.7113 1.6816 1.9259 2.2355 1.5504 1.7846 1.8752 1.7049 1.802

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Tif4632

Tif4632


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tif4632-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available