Standard name
Human Ortholog
Description Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Dam1p to connect the DASH complex with microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Bud Neck – 0.05 0 0.06 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0.06 0.05 0.05 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0.13 0.17 0.12 0.1 0.05 0.07 0.05 0.07 0 0 0.15 0.17 0.25 0.1 0.11 0.13 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0.05 0.07 0 0 0 0 0 0 0.05 0.05 0.13 0.25 0.15 0.17 0 0.07 0 0.05 0.05 0.09
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.1 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0.05 0
Nucleus – 0 0.08 0.08 0 0 0.06 0 0 0 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0.49 0.14 0.18 0.22 0.22 0.23 0.2 0.24 0.21 0.32 0.29 0.25 0.18 0.21 0.57 0.61 0.54 0 0.08 0 0.06 0 0
Peroxisomes – 0 0.07 0.05 0 0 0 0 0.05 0 0 0 0.08 0.06 0.07 0.16 0.12 0.13 0 0 0 0 0 0
SpindlePole – 0.65 0.81 0.65 0.74 0.74 0.81 0.81 0.75 0.82 0.78 0.87 0.72 0.73 0.55 0.66 0.74 0.67 1.0 0.68 0.76 0.67 0.61 0.65
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 3 0
Bud – 2 1 1 0 1 2 11 10 6 7 17 0 0 0 1 0 1 0 1 7 3 5 6
Bud Neck – 6 2 7 1 9 8 12 18 15 10 13 0 1 2 0 0 0 0 2 2 0 3 0
Bud Site – 5 1 4 1 2 7 10 16 15 14 28 0 3 2 0 2 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 17 19 22 26 14 23 15 23 8 7 13 35 51 13 16 13 0 0 1 1 6 0
Endoplasmic Reticulum – 0 0 0 0 0 1 1 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 1
Endosome – 0 4 3 9 18 9 9 4 1 8 3 4 11 28 32 22 17 0 8 4 4 11 15
Golgi – 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 0 1 0 0 2 0 2 0
Mitochondria – 11 0 3 0 6 2 3 21 9 10 14 0 2 1 9 6 5 0 3 1 2 10 1
Nucleus – 2 11 9 8 11 18 13 9 7 15 8 0 6 7 5 4 6 0 3 3 2 7 2
Nuclear Periphery – 0 0 0 1 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 1
Nucleolus – 55 19 20 39 56 68 67 66 67 88 112 21 37 43 74 89 55 0 10 4 5 8 3
Peroxisomes – 2 10 6 2 7 4 5 13 5 8 8 7 13 14 21 17 13 0 1 1 2 1 2
SpindlePole – 73 108 73 133 192 237 268 206 257 213 339 61 146 115 85 108 68 0 89 106 64 124 112
Vac/Vac Membrane – 1 1 1 4 4 3 4 2 1 2 2 4 1 9 2 0 1 0 0 0 2 3 3
Unique Cell Count 112 134 113 180 260 291 330 273 312 274 391 85 201 208 129 145 101 1 132 140 96 204 174
Labelled Cell Count 157 174 146 220 333 373 427 380 407 384 553 110 256 273 245 265 181 1 132 140 96 204 174


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.5 6.7 6.4 7.0 6.1 5.8 6.2 5.0 5.5 5.6 5.5 6.7 6.5 6.4 6.4 8.0 9.2 7.1 8.0 7.6
Std Deviation (1e-4) – 1.3 1.4 2.0 2.2 2.1 1.7 2.0 1.6 1.6 2.1 1.3 1.8 1.5 1.7 1.8 2.0 2.4 1.6 2.3 1.7
Intensity Change (Log2) – – – -0.06 0.07 -0.12 -0.19 -0.1 -0.41 -0.27 -0.26 -0.29 0.01 -0.03 -0.06 -0.05 0.26 0.46 0.09 0.26 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 2.2 1.7 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2.4 0 0 2.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 -0.1 -0.8 -2.9 -2.0 -2.5 -1.8 -3.9 -5.2 0.5 1.2 2.7 -0.7 -0.4 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.9 1.6 0 0 0 0 0 0 0 1.1 3.2 5.1 3.5 3.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.3 0 0 0 0 0 0 0 0 0
Nucleus -0.1 -1.4 -1.6 -0.8 -1.9 -2.2 -2.9 -1.1 0 0 -2.1 -2.0 -1.5 -2.0 -0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 1.7 1.8 2.2 1.5 2.3 1.8 3.9 3.3 2.0 1.0 1.5 7.3 8.1 6.6
Peroxisomes -0.7 -2.9 -2.2 0 0 -1.1 0 -2.1 0 0.2 -0.4 -0.3 2.2 1.2 1.4
SpindlePole -2.8 -1.4 -1.5 0.2 0.2 -1.2 0.4 -0.7 1.7 -1.5 -1.7 -4.8 -2.7 -1.2 -2.3
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.5045 14.5835 5.9027 11.9917 18.2672 17.787 9.0483 10.4045 6.6117 19.8696
Translational Efficiency 2.2316 1.7124 2.4852 1.2905 1.7981 2.7698 2.582 1.2882 2.3077 1.9626

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✔
Translational Efficiency ✘

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Duo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available