Standard name | |
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Human Ortholog | |
Description | RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 1 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 5 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 1 | 5 | 3 |
Bud Neck | 2 | 0 | 1 | 0 | 1 | 1 | – | 3 | 1 | 5 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 13 | 2 | 1 | 3 | – | 9 | 13 | 13 | 17 | 26 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 4 | 11 | 14 | 18 | 13 | – | 9 | 11 | 15 | 12 | 11 | 6 | 19 | 26 | 15 | 12 | 9 | 0 | 2 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 4 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 2 | 2 | 2 | 5 | 0 | 2 | 3 | 3 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 5 |
Mitochondria | 7 | 0 | 15 | 11 | 2 | 15 | – | 15 | 23 | 15 | 29 | 42 | 1 | 0 | 2 | 3 | 9 | 4 | 3 | 0 | 3 | 0 | 0 | 0 |
Nucleus | 510 | 99 | 281 | 229 | 186 | 204 | – | 279 | 265 | 237 | 269 | 265 | 138 | 133 | 122 | 74 | 54 | 84 | 519 | 106 | 252 | 117 | 138 | 147 |
Nuclear Periphery | 0 | 1 | 1 | 1 | 2 | 2 | – | 2 | 5 | 4 | 0 | 5 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 311 | 100 | 233 | 142 | 114 | 165 | – | 206 | 250 | 170 | 254 | 238 | 118 | 92 | 67 | 36 | 31 | 46 | 111 | 49 | 118 | 76 | 88 | 94 |
Peroxisomes | 2 | 0 | 1 | 1 | 0 | 0 | – | 2 | 0 | 1 | 0 | 0 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 2 | 0 | 0 | 2 | 4 | 6 | 2 | 0 | 0 | 3 | 0 | 2 | 2 | 2 | 3 |
Vac/Vac Membrane | 1 | 0 | 0 | 2 | 0 | 0 | – | 2 | 0 | 2 | 1 | 1 | 3 | 3 | 3 | 1 | 3 | 6 | 13 | 1 | 12 | 9 | 12 | 18 |
Unique Cell Count | 627 | 144 | 380 | 301 | 265 | 301 | 409 | 390 | 336 | 410 | 415 | 197 | 196 | 193 | 113 | 90 | 131 | 665 | 163 | 403 | 218 | 262 | 283 | |
Labelled Cell Count | 842 | 204 | 556 | 402 | 324 | 405 | 531 | 568 | 469 | 584 | 593 | 274 | 255 | 236 | 135 | 113 | 155 | 665 | 163 | 403 | 218 | 262 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.3 | 20.8 | 11.0 | 14.9 | 13.2 | 10.8 | – | 11.0 | 9.8 | 10.3 | 9.4 | 9.3 | 18.2 | 17.9 | 15.8 | 19.1 | 17.8 | 18.5 | 16.8 | 17.1 | 18.6 |
Std Deviation (1e-4) | 4.4 | 7.3 | 4.3 | 8.2 | 6.3 | 4.5 | – | 4.3 | 4.1 | 4.5 | 4.4 | 3.5 | 6.5 | 8.0 | 7.2 | 10.1 | 7.3 | 8.7 | 5.1 | 5.9 | 9.2 |
Intensity Change (Log2) | – | – | – | 0.44 | 0.27 | -0.02 | – | 0.0 | -0.16 | -0.09 | -0.22 | -0.24 | 0.73 | 0.71 | 0.53 | 0.8 | 0.7 | 0.75 | 0.62 | 0.64 | 0.76 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -2.4 | -2.6 | -2.1 | – | -1.0 | -0.1 | 0.3 | 0.5 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.2 | 2.4 | 1.0 | – | -0.6 | -0.1 | 1.1 | 0 | -0.2 | 0.1 | 3.5 | 4.9 | 4.3 | 0 | 2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.2 | -2.5 | 0.7 | – | -0.2 | 1.2 | 0.3 | 1.9 | 3.4 | -2.4 | -2.8 | -1.9 | 0 | 0 | 0 |
Nucleus | 0.6 | -1.1 | -1.8 | – | -1.8 | -1.8 | -1.0 | -2.5 | -3.1 | -1.0 | -1.5 | -2.7 | -1.8 | -2.6 | -2.1 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -3.7 | -4.6 | -1.7 | – | -3.1 | 0.8 | -2.9 | 0.2 | -1.1 | -0.3 | -3.3 | -6.0 | -5.5 | -4.6 | -5.2 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.8931 | -0.4118 | -0.629 | -0.7769 | -0.9796 | -0.6513 | -1.5845 | -1.2148 | -1.3854 | -1.432 | -0.7107 | -1.1054 | -0.3562 | -0.0404 | -0.0267 | -0.0849 | 0.7084 | -0.0259 |
Actin | 0.0008 | 0.0001 | 0.0006 | 0.0002 | 0 | 0.0001 | 0.009 | 0.0046 | 0.0072 | 0.0001 | 0.0521 | 0.0026 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud | 0.0002 | 0.0018 | 0.0012 | 0.0001 | 0 | 0.0026 | 0.0015 | 0.0043 | 0.0007 | 0 | 0.0018 | 0.0006 | 0 | 0 | 0.0048 | 0 | 0 | 0 |
Bud Neck | 0.0002 | 0.0015 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0027 | 0.0004 | 0.0003 | 0 | 0.0134 | 0.0022 | 0 | 0.0001 | 0.0008 | 0 | 0 | 0.0001 |
Bud Periphery | 0.0002 | 0.0011 | 0.0013 | 0.0001 | 0 | 0.0026 | 0.0051 | 0.0043 | 0.0007 | 0.0001 | 0.0039 | 0.0012 | 0 | 0 | 0.0048 | 0 | 0 | 0 |
Bud Site | 0.0034 | 0.017 | 0.0118 | 0.0005 | 0 | 0.0003 | 0.0055 | 0.0117 | 0.0067 | 0.0001 | 0.0107 | 0.0005 | 0 | 0.0004 | 0.004 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 | 0.0005 | 0.0005 | 0.0001 | 0 | 0.0011 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0171 | 0.0231 | 0.0136 | 0.0158 | 0.0078 | 0.0203 | 0.0238 | 0.028 | 0.0137 | 0.0124 | 0.0108 | 0.0264 | 0.0066 | 0.0259 | 0.0112 | 0.0065 | 0.01 | 0.0175 |
Cytoplasmic Foci | 0.0055 | 0.0094 | 0.0039 | 0.0036 | 0.0068 | 0.0066 | 0.0124 | 0.0064 | 0.0137 | 0.0038 | 0.0104 | 0.0057 | 0.0006 | 0.0016 | 0.0089 | 0.0009 | 0.0012 | 0.0017 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0017 | 0.0005 | 0.0003 | 0.0002 | 0.002 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Endosome | 0.0013 | 0.0016 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0039 | 0.0003 | 0.0003 | 0.0002 | 0.0114 | 0.0021 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0001 |
Golgi | 0.0004 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0048 | 0.001 | 0 | 0 | 0.0002 | 0 | 0 | 0 |
Lipid Particles | 0.0013 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0037 | 0.0004 | 0.0005 | 0.0002 | 0.0081 | 0.0028 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Mitochondria | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0009 | 0.0005 | 0.0002 | 0.0024 | 0.0021 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 |
None | 0.9657 | 0.9336 | 0.9561 | 0.9739 | 0.978 | 0.9624 | 0.9144 | 0.9219 | 0.9483 | 0.9797 | 0.8002 | 0.9343 | 0.9921 | 0.97 | 0.9593 | 0.9915 | 0.9878 | 0.9756 |
Nuclear Periphery | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0022 | 0.0011 | 0.0003 | 0.0001 | 0.0099 | 0.0016 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Nucleolus | 0.0003 | 0.0005 | 0.0008 | 0.0006 | 0.0004 | 0.0002 | 0.0008 | 0.0012 | 0.0005 | 0.0004 | 0.0066 | 0.0007 | 0 | 0.0001 | 0.0009 | 0 | 0 | 0.0003 |
Nucleus | 0.0005 | 0.0012 | 0.0022 | 0.0007 | 0.0008 | 0.0013 | 0.0015 | 0.0054 | 0.0009 | 0.0004 | 0.0063 | 0.0025 | 0 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0008 |
Peroxisomes | 0.0007 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.001 | 0.0047 | 0.0008 | 0.002 | 0.0001 | 0.0047 | 0.0077 | 0 | 0.0001 | 0.0006 | 0 | 0 | 0 |
Punctate Nuclear | 0.001 | 0.0067 | 0.0059 | 0.0024 | 0.0052 | 0.0011 | 0.003 | 0.0064 | 0.0026 | 0.0014 | 0.0319 | 0.0042 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0 | 0.0033 |
Vacuole | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0009 | 0.0005 | 0.0002 | 0.0002 | 0.0054 | 0.0005 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.8928 | 42.7697 | 33.7205 | 38.8197 | 42.647 | 32.8746 | 46.6229 | 36.4838 | 37.6124 | 41.016 |
Translational Efficiency | 3.7075 | 2.9467 | 3.2037 | 2.5188 | 2.5497 | 2.996 | 2.2344 | 2.504 | 2.1408 | 2.5486 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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