Standard name
Human Ortholog
Description Putative kinase; suppressor of GTPase mutant; similar to bovine rhodopsin kinase; may have a role in intracellular sterol transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.1 0 0 0 0.06 0.06 0.06
Bud 0.06 0.06 0.07 0.08 0.05 0.06 0.13 0.13 0.11 0.08 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0.06 0.1 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.08 0.09 0 0.08 0.1 0.09 0.11 0.09 0.09 0.11 0.08 0.06 0.07 0.09 0.12 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0.11 0.32 0.15 0.3 0.27 0.11 0.11 0 0 0 0 0.23 0.42 0.38 0.31 0.32 0.27 0.29 0.41 0.33 0.27 0.28 0.28
Golgi 0.06 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.08 0.28 0.37 0.36 0.35 0.18 0.17 0.23 0.26 0.29
Mitochondria 0.86 0.73 0.3 0.62 0.38 0.54 0.59 0.48 0.75 0.76 0.78 0.74 0.66 0.42 0.37 0.13 0.18 0.14 0.09 0.14 0.21 0.16 0.13 0.12
Nucleus 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0.1 0.12 0.25 0.19 0.21 0.15 0.09 0 0.05 0.08 0.12 0.19 0.15 0 0 0 0.18 0.1 0.14 0.2 0.21 0.19
SpindlePole 0.08 0.09 0.26 0.23 0.28 0.19 0.29 0.32 0.2 0.26 0.17 0.29 0.07 0.12 0.07 0.24 0.19 0.23 0 0.08 0 0 0 0
Vac/Vac Membrane 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 5 2 0 0 4 3 1 2 0 0 7 2 3 16 13 16 2 10 5 14 23 22
Bud 4 13 12 5 10 16 42 36 32 26 18 20 7 7 10 1 0 6 0 2 1 1 1 2
Bud Neck 0 0 0 0 1 1 0 2 2 0 0 2 0 0 0 0 0 0 0 0 1 2 1 0
Bud Site 0 0 0 1 0 4 10 12 15 20 23 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 2 1 1 0 0 1 1 1 1 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 17 16 2 15 26 31 32 26 30 26 15 13 25 31 21 10 14 0 0 3 0 1 0
Endoplasmic Reticulum 2 9 2 1 0 0 0 1 0 0 0 0 10 4 8 7 3 3 0 0 2 1 3 2
Endosome 5 25 56 10 56 72 36 30 11 2 4 7 52 150 137 55 56 44 25 113 73 65 112 105
Golgi 4 4 6 0 3 0 0 1 0 0 0 0 13 22 28 51 64 59 30 49 37 55 107 110
Mitochondria 55 162 53 41 70 141 197 138 216 243 187 132 149 151 133 24 31 22 7 38 47 40 53 44
Nucleus 0 1 1 4 1 4 11 6 4 7 5 6 1 1 2 0 1 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 5 8 0 2 0 1 3 10 8 12 7 5 1 4 7 3 2 2 0 1 0 0 0 0
Peroxisomes 3 5 18 8 47 51 69 42 27 7 13 15 28 68 56 3 3 1 15 26 30 48 84 72
SpindlePole 5 20 45 15 52 51 98 92 56 85 41 52 15 44 27 43 33 38 2 22 7 7 10 6
Vac/Vac Membrane 3 7 13 2 4 8 4 5 4 6 2 3 8 6 17 10 14 11 1 7 4 5 4 4
Unique Cell Count 64 223 174 66 186 263 334 285 287 321 240 178 227 357 362 179 175 162 88 276 219 246 408 375
Labelled Cell Count 92 273 228 93 261 376 506 410 402 442 327 267 306 486 459 234 230 217 88 276 219 246 408 375


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 5.2 6.3 5.5 6.4 5.4 5.2 5.6 4.6 4.4 4.2 4.5 5.8 5.9 6.2 8.8 9.5 8.3 5.4 5.6 5.9
Std Deviation (1e-4) 0.6 0.7 1.2 1.8 1.7 1.7 1.5 1.7 1.4 1.2 1.3 1.1 0.8 1.0 1.0 1.3 1.8 1.5 0.8 1.0 1.3
Intensity Change (Log2) – – – -0.19 0.02 -0.23 -0.29 -0.17 -0.46 -0.54 -0.59 -0.49 -0.12 -0.09 -0.03 0.48 0.59 0.39 -0.22 -0.18 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0.1 0 0 2.4 1.9 2.6
Bud 0 -0.6 -0.3 2.0 1.9 0 0 0 0 -1.8 -2.9 -2.3 -3.2 -3.5 -1.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.4 0.2 0 0.7 0 0.1 0.5 -0.3 -1.3 -0.9 -0.2 0.8 -1.2 -0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1.9 0 0 0 0 0
Endosome -2.6 -0.4 -1.1 -5.9 -5.8 0 0 0 0 -2.1 2.2 1.3 -0.3 0 -1.0
Golgi 0 0 0 0 0 0 0 0 0 1.1 1.3 1.9 6.4 7.7 7.6
Mitochondria 4.5 1.4 4.8 6.1 3.8 0 0 0 0 7.0 2.6 1.4 -3.9 -2.8 -3.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.4 3.7 2.5 2.9 1.4 0 0 0 0 0.6 2.6 1.6 -3.4 -3.4 -3.9
SpindlePole -0.5 0.4 -1.6 0.8 1.5 0 0 0 0 -5.4 -3.9 -5.9 -0.4 -1.6 -0.5
Vacuole 0 -2.4 -2.1 -3.7 -3.1 -3.4 -3.1 -3.6 -2.6 -1.8 -3.4 -1.3 -0.7 0.2 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9655 3.5479 3.1167 2.7085 2.6308 3.0835 0.6708 1.1057 0.9923 1.0979 0.5124 0.9443 0.4008 1.2841 0.9543 0.8544 1.0257 0.9092
Actin 0.032 0.0191 0.0114 0.0018 0.0431 0.0168 0.0333 0.0364 0.0213 0.0031 0.0177 0.0105 0.0327 0.0171 0.0245 0.0042 0.063 0.0192
Bud 0.0003 0.001 0.0003 0.0001 0.0003 0.0002 0.0004 0.0009 0.0008 0.0008 0.0002 0.0001 0.0004 0.0002 0.0003 0.0003 0.0007 0.0002
Bud Neck 0.0015 0.0037 0.0017 0.001 0.0023 0.0022 0.0012 0.0008 0.0009 0.001 0.0004 0.001 0.001 0.0005 0.0013 0.001 0.0013 0.0009
Bud Periphery 0.0004 0.0014 0.0003 0.0001 0.0005 0.0005 0.0004 0.0006 0.0009 0.0012 0.0003 0.0001 0.0004 0.0001 0.0002 0.0002 0.0014 0.0003
Bud Site 0.0026 0.0241 0.0114 0.0005 0.0009 0.0009 0.002 0.0098 0.0065 0.003 0.0007 0.0004 0.003 0.0009 0.001 0.0008 0.0015 0.0004
Cell Periphery 0.0004 0.0012 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0 0.0002 0.0001 0 0.0001 0.0003 0.0001
Cytoplasm 0.0201 0.0125 0.0526 0.0033 0.0072 0.0105 0.013 0.0196 0.0086 0.0034 0.0026 0.0041 0.0167 0.0291 0.0049 0.0226 0.0037 0.0024
Cytoplasmic Foci 0.2254 0.2608 0.1791 0.2411 0.1741 0.1902 0.2195 0.2812 0.2069 0.1469 0.231 0.1992 0.2694 0.2487 0.2239 0.2205 0.1702 0.1879
Eisosomes 0.0016 0.0011 0.0002 0 0.0004 0.0002 0.0002 0.0001 0.0001 0 0.0002 0.0002 0.0002 0.0001 0.0001 0 0.0002 0.0002
Endoplasmic Reticulum 0.0032 0.0019 0.003 0.0002 0.0023 0.0004 0.0032 0.0013 0.0008 0.0008 0.0009 0.0003 0.0046 0.0036 0.0006 0.0012 0.0014 0.0011
Endosome 0.1856 0.2535 0.1843 0.2768 0.2555 0.1707 0.327 0.2453 0.2753 0.3878 0.3037 0.2547 0.2712 0.3463 0.2894 0.3406 0.3994 0.2307
Golgi 0.1152 0.1637 0.1528 0.121 0.162 0.1607 0.2 0.188 0.2334 0.2456 0.2494 0.3016 0.1455 0.2021 0.254 0.2273 0.1615 0.2874
Lipid Particles 0.1054 0.0337 0.0456 0.0687 0.0301 0.0933 0.0469 0.0196 0.0327 0.012 0.0374 0.0344 0.0708 0.0208 0.0335 0.0541 0.0946 0.076
Mitochondria 0.0564 0.0499 0.0516 0.0596 0.0237 0.0464 0.0208 0.0209 0.0338 0.0328 0.016 0.0192 0.007 0.0067 0.0103 0.009 0.0143 0.0383
None 0.0456 0.0226 0.0206 0.0008 0.0048 0.0102 0.0084 0.005 0.0019 0.0002 0.0023 0.0002 0.0249 0.0118 0.0142 0.0011 0.0009 0.0004
Nuclear Periphery 0.002 0.0006 0.0077 0.0001 0.0006 0.0005 0.0049 0.0004 0.0006 0.0004 0.0036 0.0001 0.0044 0.0007 0.0002 0.0004 0.0008 0.0006
Nucleolus 0.0016 0.0007 0.0011 0.0002 0.0002 0.0004 0.0003 0.0005 0.0004 0.0002 0.0006 0.0001 0.0003 0.0001 0.0001 0.0001 0.0005 0.0001
Nucleus 0.001 0.0006 0.0787 0.0001 0.0002 0.0075 0.0007 0.0003 0.0005 0.0004 0.0005 0.0001 0.0013 0.0005 0.0001 0.0002 0.0004 0.0001
Peroxisomes 0.1866 0.1409 0.1906 0.2202 0.2857 0.2795 0.1036 0.1592 0.163 0.1503 0.1258 0.171 0.1366 0.1051 0.1324 0.1093 0.0483 0.1492
Punctate Nuclear 0.0087 0.0011 0.0024 0.0015 0.0007 0.0056 0.0047 0.002 0.0007 0.0003 0.0029 0.0004 0.0043 0.0012 0.0056 0.0011 0.0022 0.001
Vacuole 0.0029 0.0047 0.0027 0.0016 0.0036 0.0021 0.0067 0.0069 0.0088 0.0063 0.0019 0.0012 0.0041 0.003 0.0023 0.0042 0.0302 0.0018
Vacuole Periphery 0.0013 0.0012 0.0016 0.0012 0.0016 0.0009 0.0025 0.001 0.0021 0.0032 0.002 0.0009 0.0013 0.0013 0.001 0.0019 0.0032 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.8534 11.8447 7.2739 9.5232 7.1086 5.9364 13.7313 8.2225 6.9837 11.6839
Translational Efficiency 1.0447 0.7166 0.7162 0.436 0.7946 1.0496 0.6746 0.7591 0.779 0.6482

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative kinase; suppressor of GTPase mutant; similar to bovine rhodopsin kinase; may have a role in intracellular sterol transport
Localization
Cell Percentages mitochondrion (13%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Scy1

Scy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available