Standard name
Human Ortholog
Description Essential cytoplasmic iron-sulfur cluster binding protein; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.97 0.99 0.99 0.93 0.86 0.75 0.72 0.66 0.69 0.66 0.67 0.98 0.99 0.99 0.99 0.94 0.98 0.95 0.95 0.97 0.93 0.91 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.1 0.24 0.36 0.31 0.46 0.37 0.48 0.5 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0.09 0.07 0.1 0.06 0 0 0 0 0 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 0 0 3 10 6 11 11 10 13 17 0 0 0 1 0 0 0 2 0 0 1 5
Bud Neck 0 0 0 0 0 0 0 0 1 0 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 1 0 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 2 1 1 3 5 3 4 1 7 1 3 2 3 4 5 0 0 0 0 1 0
Cytoplasm 75 149 122 383 584 582 683 678 416 699 399 625 232 348 480 231 172 220 84 154 115 212 247 309
Endoplasmic Reticulum 0 0 0 1 0 2 10 4 0 1 2 2 3 2 3 12 5 4 0 0 0 1 0 6
Endosome 0 0 0 0 0 0 0 2 0 5 1 1 0 0 0 1 0 0 0 1 0 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 3
Mitochondria 1 3 4 7 61 161 330 287 289 370 291 471 0 1 0 1 2 1 0 1 0 2 3 8
Nucleus 0 1 0 2 0 11 14 25 12 17 14 18 2 1 4 5 4 4 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 2 1 9 14 5 16 8 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 3 0 4 1 0 0 0 0 0 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 1 0 0 0 1 0 0 5 0 0 0 0 0 0 0 1 0 0 0 3
Vac/Vac Membrane 0 1 1 4 25 18 61 80 47 100 37 42 1 3 5 0 12 2 0 0 0 6 5 8
Unique Cell Count 75 154 123 388 629 675 911 936 628 1010 606 937 237 353 487 234 183 225 89 163 119 228 272 358
Labelled Cell Count 76 155 127 399 677 788 1117 1110 786 1227 768 1192 239 358 495 254 201 236 89 163 119 228 272 358


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.8 5.1 5.4 5.0 4.6 4.1 4.3 4.1 4.1 4.0 3.8 7.1 7.3 7.0 7.7 7.6 7.9 8.2 7.5 7.7
Std Deviation (1e-4) 1.8 1.8 1.1 1.3 1.2 1.2 1.2 1.0 1.0 1.0 1.2 1.1 1.7 1.6 1.4 1.3 1.5 1.3 1.6 1.8 1.7
Intensity Change (Log2) – – – 0.06 -0.05 -0.16 -0.32 -0.25 -0.34 -0.33 -0.37 -0.43 0.47 0.5 0.44 0.58 0.57 0.62 0.67 0.55 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -2.7 -4.1 -6.1 -6.5 -7.4 -7.0 -7.5 -7.4 -0.9 -0.5 -0.5 -0.4 -2.3 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.6 3.0 2.8 3.3 2.4 0 0 0 0 0 2.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4356 1.8347 1.6096 1.6034 1.1696 1.7405 1.4191 2.603 1.8658 2.334 2.1012 1.9126 -0.2927 0.5381 0.5286 0.1219 -1.3724 0.4637
Actin 0.0182 0.0004 0.0027 0.0093 0.0063 0.0018 0.0064 0.0002 0.0234 0.0002 0.001 0.0103 0.0005 0.0013 0.0014 0.0918 0.0005 0.0059
Bud 0.0013 0.001 0.0022 0.0086 0.0003 0.0015 0.0001 0.0001 0.0016 0 0 0.0003 0 0.0003 0.0001 0.0017 0 0.0005
Bud Neck 0.0044 0.0003 0.0012 0.0004 0.0007 0.0067 0.0001 0.0001 0.0077 0.0001 0.0002 0.0005 0.0003 0.0002 0.0001 0.0071 0.0001 0.0004
Bud Periphery 0.0025 0.0005 0.0019 0.0049 0.0006 0.0007 0.0002 0 0.0013 0 0 0.0004 0 0.0005 0 0.0022 0 0.0005
Bud Site 0.0038 0.0037 0.0024 0.004 0.0006 0.0003 0.0003 0.0002 0.0036 0 0.0001 0.0006 0.0008 0.001 0.0001 0.0115 0 0.0006
Cell Periphery 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0007 0 0 0.0001 0 0.0012 0 0.0022 0.0001 0.0001
Cytoplasm 0.4239 0.6002 0.5698 0.5704 0.5693 0.5287 0.4569 0.5378 0.4938 0.3349 0.5567 0.498 0.3728 0.7349 0.6198 0.4971 0.5691 0.6161
Cytoplasmic Foci 0.0212 0.0077 0.0101 0.0092 0.0116 0.0182 0.0213 0.0022 0.0113 0.0025 0.0189 0.0078 0.2756 0.0037 0.0395 0.0606 0.0024 0.0154
Eisosomes 0.0002 0 0 0.0002 0.0002 0 0.0002 0.0001 0.0002 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0001
Endoplasmic Reticulum 0.0046 0.003 0.0021 0.0021 0.0025 0.0021 0.0085 0.0009 0.0032 0.0037 0.0009 0.0014 0.0049 0.0065 0.0019 0.0009 0.0037 0.0027
Endosome 0.0163 0.0031 0.0034 0.0064 0.0134 0.0046 0.0109 0.0004 0.0059 0.0018 0.0014 0.0025 0.0291 0.0021 0.0061 0.065 0.0006 0.0519
Golgi 0.0028 0.0002 0.0006 0.0018 0.0046 0.0009 0.0039 0.0001 0.0035 0.0001 0.0009 0.0024 0.0061 0.0003 0.0068 0.041 0.0001 0.0296
Lipid Particles 0.003 0.0001 0.0003 0.0069 0.0033 0.0011 0.0033 0.0001 0.0018 0.0001 0.0022 0.0011 0.0558 0.0016 0.0064 0.0141 0.0001 0.0076
Mitochondria 0.0016 0.0003 0.0006 0.0029 0.0253 0.0005 0.0015 0.0001 0.0078 0.0001 0.0002 0.0036 0.0003 0.0001 0.0004 0.0161 0 0.0331
None 0.4604 0.3678 0.3861 0.3395 0.3424 0.407 0.4597 0.4557 0.4236 0.654 0.3743 0.4651 0.199 0.24 0.2958 0.0032 0.4223 0.223
Nuclear Periphery 0.0088 0.0014 0.0013 0.0041 0.0066 0.0054 0.0016 0.0003 0.0011 0.0006 0.0003 0.0004 0.0011 0.0015 0.0007 0.0005 0.0003 0.0028
Nucleolus 0.0012 0.0001 0.0002 0.0021 0.0005 0.0005 0.0001 0.0002 0.0001 0 0 0.0001 0.0002 0.0001 0 0.0066 0 0.0002
Nucleus 0.0057 0.0064 0.0083 0.0125 0.0046 0.0107 0.0057 0.0009 0.0022 0.0006 0.0007 0.0017 0.0008 0.0019 0.0008 0.0031 0.0002 0.0021
Peroxisomes 0.0095 0.0003 0.003 0.0022 0.0014 0.0024 0.0173 0 0.0051 0 0.0408 0.0025 0.0388 0.0001 0.0189 0.0379 0 0.0031
Punctate Nuclear 0.007 0.0021 0.0022 0.0102 0.0041 0.0053 0.0007 0.0003 0.001 0.0002 0.0006 0.0007 0.0121 0.0006 0.0006 0.0006 0.0001 0.0008
Vacuole 0.0026 0.0011 0.0014 0.0014 0.0011 0.0011 0.0008 0.0003 0.001 0.0006 0.0004 0.0004 0.0014 0.0021 0.0004 0.1218 0.0002 0.0017
Vacuole Periphery 0.0007 0.0002 0.0002 0.0007 0.0007 0.0003 0.0003 0 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0148 0 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0922 19.9874 20.7641 23.3013 26.0902 20.1117 21.4723 24.2107 26.3436 20.5051
Translational Efficiency 2.349 2.2156 1.5195 1.86 1.3169 2.3652 2.0404 1.7841 1.7261 1.6945

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nbp35-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available