Standard name
Human Ortholog
Description Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0.07 0.15 0.13 0.11 0.14 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0.18 0.15 0.12 0.47 0.39 0.34 – 0.54 0.58 0.49 0 0 0 0.11 0.07 0.07 0 0 0 0.1 0.17 0.17
Nucleus 0 0.05 0 0.09 0 0 0 0.05 – 0.06 0.06 0 0 0 0 0.12 0.18 0.1 0 0 0 0 0 0
Nuclear Periphery 0.8 0.82 0.78 0.74 0.75 0.59 0.63 0.59 – 0.51 0.49 0.59 0.79 0.75 0.66 0.53 0.5 0.5 0.88 0.83 0.87 0.77 0.65 0.65
Nucleolus 0.12 0.1 0.24 0.26 0.26 0.29 0.3 0.37 – 0.35 0.34 0.38 0.07 0.08 0.1 0.17 0.12 0.15 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.3 0.23 0.15 0.07 0.15 0.05 0.06 0.05 – 0.05 0 0 0.17 0.21 0.26 0.3 0.2 0.23 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 0 0 0 0 0 – 0 0 0 2 0 3 1 0 0 4 0 1 4 5 8
Bud 0 0 0 0 0 0 1 3 – 5 1 3 0 0 0 0 0 0 8 5 1 3 4 1
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 4 0 1 – 9 3 2 0 3 2 1 1 0 – – – – – –
Cell Periphery 3 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 8 4 11 6 8 8 5 9 – 5 4 1 20 23 38 24 39 24 0 0 0 0 0 1
Endoplasmic Reticulum 12 0 2 1 3 4 5 5 – 5 3 4 2 0 2 8 8 5 0 0 2 0 0 1
Endosome 1 0 11 2 17 6 7 8 – 3 1 4 1 0 4 1 6 11 5 7 4 5 25 18
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 1 1 1 1 3 1 1 5 7 10 9
Mitochondria 10 45 76 39 39 206 204 187 – 315 210 228 3 0 2 24 20 13 7 14 14 40 95 83
Nucleus 16 23 6 22 10 18 23 25 – 32 23 12 5 4 9 26 51 19 4 7 2 3 5 2
Nuclear Periphery 402 353 322 187 240 259 330 321 – 297 178 274 214 118 200 111 144 98 501 392 379 322 359 314
Nucleolus 58 44 101 66 85 125 158 200 – 202 124 175 19 12 30 36 36 29 11 12 8 8 10 4
Peroxisomes 0 0 0 0 0 2 1 0 – 1 1 3 0 1 1 0 1 1 0 0 0 4 8 6
SpindlePole 1 0 2 2 0 5 3 1 – 2 1 3 7 7 13 1 1 1 2 2 3 2 2 2
Vac/Vac Membrane 152 99 61 18 48 21 33 26 – 28 15 19 45 33 79 62 57 45 21 25 9 10 22 17
Unique Cell Count 501 432 413 253 322 438 525 546 581 363 466 272 157 302 210 288 195 573 473 438 417 555 480
Labelled Cell Count 665 568 592 343 451 658 770 786 904 564 729 319 202 384 296 367 249 573 473 438 417 555 480


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.2 14.0 12.1 10.7 10.7 8.5 9.0 8.9 – 7.5 7.6 8.4 13.1 12.5 13.0 10.2 9.8 12.6 14.3 14.8 15.6
Std Deviation (1e-4) 5.6 3.7 3.5 3.2 3.5 2.8 3.2 3.3 – 2.9 2.9 3.6 4.4 4.2 4.9 4.4 4.9 5.8 3.2 3.4 4.0
Intensity Change (Log2) – – – -0.19 -0.18 -0.52 -0.43 -0.46 – -0.7 -0.68 -0.54 0.11 0.05 0.1 -0.26 -0.31 0.06 0.24 0.29 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -0.2 -0.8 -2.0 -1.1 – -2.2 -1.6 -3.1 2.9 5.4 5.2 4.5 5.5 4.8
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 1.8 -1.3 -1.5 -1.3 – -2.8 -2.7 -2.1 0 0 -1.2 0 -0.5 1.8
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 -2.3 8.9 6.8 5.4 – 11.4 11.4 9.5 -6.9 -5.8 -7.5 -2.2 -4.3 -3.8
Nucleus 4.5 1.5 2.3 2.6 2.7 – 3.3 3.6 1.2 0 0 1.4 5.8 7.7 4.8
Nuclear Periphery -1.2 -1.1 -5.9 -5.0 -6.3 – -8.6 -8.4 -6.1 0.2 -0.7 -3.5 -6.4 -7.7 -6.9
Nucleolus 0.5 0.6 1.3 1.9 4.0 – 3.5 3.0 4.2 -5.9 -4.5 -5.0 -2.1 -3.9 -2.7
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 3.5 0 0 0
Vacuole -3.0 0.1 -4.9 -4.3 -5.3 – -5.4 -5.0 -5.5 0.6 1.8 3.8 4.4 1.7 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0204 0.0004 0.0259 0.0415 0.0014 0.0016 0.0143 0.0001 0.0026 0.0069 0.0003 0 – – – – – –
Bud 0.0003 0 0.0001 0.0001 0 0.0001 0.0003 0 0.0001 0.0001 0 0 – – – – – –
Bud Neck 0.0006 0 0.0004 0.0001 0.0001 0.0001 0.0006 0 0.0001 0.0001 0 0 – – – – – –
Bud Periphery 0.0004 0.0001 0.0004 0.0004 0.0003 0.0001 0.0006 0 0.0004 0.0004 0 0 – – – – – –
Bud Site 0.0011 0.0002 0.0003 0.0004 0.0002 0.0002 0.0011 0 0.0003 0.0001 0 0 – – – – – –
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0 – – – – – –
Cytoplasm 0.0098 0.0007 0.0006 0.0007 0.0001 0.001 0.0077 0 0.0002 0.0001 0 0 – – – – – –
Cytoplasmic Foci 0.0033 0.0003 0.009 0.0031 0.0078 0.0065 0.019 0 0.0014 0.0041 0 0.0001 – – – – – –
Eisosomes 0.0003 0 0.0002 0.0002 0.0001 0 0.0002 0 0.0001 0.0001 0 0 – – – – – –
Endoplasmic Reticulum 0.0041 0.0004 0.0018 0.0018 0.0004 0.0003 0.0085 0.0003 0.0019 0.0013 0.0002 0.0001 – – – – – –
Endosome 0.0197 0.0004 0.0044 0.003 0.0027 0.001 0.0617 0.0001 0.003 0.0017 0.0002 0.0001 – – – – – –
Golgi 0.0056 0.0001 0.0033 0.0029 0.0027 0.0017 0.0137 0.0001 0.0013 0.0041 0.0001 0.0002 – – – – – –
Lipid Particles 0.0113 0.0007 0.0076 0.003 0.0216 0.0026 0.0163 0.0007 0.0178 0.0672 0.0011 0.004 – – – – – –
Mitochondria 0.0056 0.0016 0.0036 0.0435 0.0077 0.0105 0.007 0.0009 0.0098 0.0057 0.0017 0.0002 – – – – – –
None 0.0044 0.0002 0.0009 0.0009 0.0004 0.0011 0.0043 0.0001 0.0005 0.0004 0.0001 0 – – – – – –
Nuclear Periphery 0.855 0.8514 0.8301 0.7987 0.805 0.91 0.7571 0.9472 0.8888 0.8641 0.9479 0.9778 – – – – – –
Nucleolus 0.0016 0.008 0.002 0.0006 0.0094 0.0032 0.0028 0.0002 0.0024 0.0007 0.0006 0.0002 – – – – – –
Nucleus 0.0155 0.0871 0.0654 0.0577 0.0651 0.0383 0.0219 0.0173 0.0195 0.0036 0.0137 0.0021 – – – – – –
Peroxisomes 0.0019 0 0.0083 0.0111 0.0288 0.0033 0.0064 0 0.0008 0.001 0 0 – – – – – –
Punctate Nuclear 0.0108 0.0063 0.003 0.0046 0.0027 0.0019 0.0194 0.0003 0.0011 0.0022 0.0003 0.0003 – – – – – –
Vacuole 0.0016 0.0023 0.0015 0.0006 0.0023 0.0008 0.0032 0.0002 0.0016 0.0004 0.0002 0.0001 – – – – – –
Vacuole Periphery 0.0265 0.0396 0.0311 0.0254 0.0412 0.0156 0.0336 0.0325 0.0463 0.0358 0.0336 0.0148 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.7428 41.1614 37.7629 44.8021 45.2267 36.2578 48.9329 46.22 41.366 53.4846
Translational Efficiency 1.1538 0.9957 1.0037 0.782 0.8988 1.0419 0.8453 0.7394 0.8299 0.7955

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
Localization
Cell Percentages nuclear periphery (90%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nup145

Nup145


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup145-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available