Standard name | |
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Human Ortholog | |
Description | Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p; human homolog of the RAN GEF, RCC1, can complement a temperature sensitive point mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 2 | 5 | 3 | 2 | 1 | 4 | 5 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 0 | 9 | 8 | 14 | 8 | 12 | 23 | 23 | 15 | 13 | 8 | 10 | 28 | 46 | 7 | 8 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 13 | 3 | 8 | 5 | 5 | 13 | 33 | 28 | 37 | 35 | 25 | 24 | 3 | 5 | 4 | 31 | 33 | 9 | 0 | 0 | 2 | 1 | 0 | 2 |
Nucleus | 180 | 32 | 94 | 129 | 202 | 181 | 306 | 277 | 220 | 256 | 161 | 142 | 177 | 355 | 342 | 182 | 112 | 119 | 147 | 58 | 80 | 100 | 176 | 203 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 132 | 26 | 77 | 72 | 74 | 105 | 225 | 195 | 208 | 234 | 177 | 139 | 130 | 265 | 232 | 96 | 62 | 66 | 48 | 7 | 35 | 76 | 136 | 158 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 7 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 10 | 19 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 6 |
Vac/Vac Membrane | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 3 | 0 | 0 | 1 | 4 | 5 | 1 | 3 | 0 | 2 | 2 | 2 | 3 | 15 | 33 |
Unique Cell Count | 205 | 40 | 128 | 159 | 238 | 231 | 433 | 397 | 342 | 376 | 263 | 211 | 218 | 472 | 487 | 209 | 148 | 146 | 204 | 70 | 132 | 186 | 339 | 418 |
Labelled Cell Count | 330 | 62 | 188 | 214 | 297 | 309 | 583 | 531 | 495 | 550 | 381 | 315 | 323 | 679 | 664 | 320 | 220 | 202 | 204 | 70 | 132 | 186 | 339 | 418 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.7 | 20.0 | 19.6 | 21.4 | 21.9 | 18.5 | 16.8 | 16.5 | 18.0 | 16.4 | 16.7 | 17.3 | 25.9 | 26.5 | 25.2 | 39.4 | 40.2 | 43.8 | 24.4 | 22.8 | 23.9 |
Std Deviation (1e-4) | 8.6 | 7.1 | 9.7 | 8.2 | 9.6 | 8.1 | 7.7 | 7.0 | 9.7 | 8.9 | 9.6 | 9.0 | 9.3 | 11.5 | 13.5 | 18.0 | 20.8 | 22.8 | 9.6 | 7.7 | 9.5 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.16 | -0.08 | -0.23 | -0.25 | -0.12 | -0.26 | -0.24 | -0.18 | 0.4 | 0.43 | 0.36 | 1.01 | 1.03 | 1.16 | 0.31 | 0.22 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | -0.4 | -1.5 | 0 | -0.5 | -0.1 | -1.4 | -0.8 | -1.3 | -1.0 | -0.5 | 0.9 | -1.5 | -0.6 | -1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.3 | 0 | -0.2 | 0.5 | 0.3 | 1.5 | 1.1 | 1.1 | 1.6 | 0 | 0 | 0 | 2.4 | 3.7 | 0 |
Nucleus | 1.6 | 2.7 | 1.1 | -0.6 | -0.8 | -1.9 | -1.1 | -2.4 | -1.2 | 1.7 | 0.4 | -0.7 | 3.2 | 0.4 | 1.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.5 | -5.4 | -2.7 | -1.6 | -2.2 | 0.1 | 0.4 | 1.4 | 1.1 | -0.1 | -0.8 | -2.5 | -2.5 | -3.0 | -2.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.3908 | 22.6727 | 20.6937 | 22.9747 | 17.0677 | 21.4486 | 21.0814 | 20.1817 | 22.0225 | 22.4883 | 18.6424 | 18.4352 | 31.6632 | 27.421 | 24.2836 | 24.5127 | 26.043 | 24.8273 |
Actin | 0.0222 | 0 | 0.019 | 0.0281 | 0.0099 | 0.0002 | 0.0122 | 0 | 0.0031 | 0 | 0.0302 | 0.0002 | 0.012 | 0.0019 | 0.0024 | 0.0084 | 0.0216 | 0.0007 |
Bud | 0.0004 | 0 | 0.0003 | 0.0003 | 0.0006 | 0.0001 | 0.0009 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0 |
Bud Neck | 0.0006 | 0 | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0.0004 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Bud Periphery | 0.0006 | 0 | 0.0004 | 0.0002 | 0.0008 | 0.0002 | 0.0011 | 0 | 0.0002 | 0 | 0.0012 | 0 | 0.0008 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0 |
Bud Site | 0.0016 | 0 | 0.0015 | 0.002 | 0.0012 | 0.0001 | 0.0013 | 0 | 0.0002 | 0 | 0.0017 | 0 | 0.001 | 0.001 | 0.0005 | 0.001 | 0.0004 | 0.0001 |
Cell Periphery | 0.0004 | 0 | 0.0002 | 0 | 0.0016 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 |
Cytoplasm | 0.0047 | 0 | 0.0003 | 0.008 | 0.0031 | 0 | 0.003 | 0 | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0009 | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0153 | 0 | 0.0077 | 0.0043 | 0.0212 | 0.0006 | 0.0041 | 0 | 0.0006 | 0 | 0.001 | 0.0001 | 0.0092 | 0.0004 | 0.0058 | 0.0052 | 0.0048 | 0.0005 |
Eisosomes | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0024 | 0 | 0.0002 | 0.0087 | 0.0036 | 0.0001 | 0.0015 | 0 | 0.0005 | 0 | 0.0007 | 0 | 0.0019 | 0.0005 | 0.0004 | 0.0005 | 0.0001 | 0.0001 |
Endosome | 0.0118 | 0 | 0.0013 | 0.0073 | 0.0232 | 0.0003 | 0.0135 | 0 | 0.0047 | 0 | 0.0035 | 0.0002 | 0.0054 | 0.0035 | 0.0043 | 0.0052 | 0.0023 | 0.0018 |
Golgi | 0.0041 | 0 | 0.002 | 0.0038 | 0.0043 | 0 | 0.0041 | 0 | 0.0038 | 0 | 0.0031 | 0 | 0.0032 | 0.0013 | 0.0014 | 0.0032 | 0.0067 | 0.001 |
Lipid Particles | 0.0079 | 0 | 0.0085 | 0.0009 | 0.023 | 0.0001 | 0.0029 | 0 | 0.007 | 0 | 0.0027 | 0.0001 | 0.0062 | 0.0003 | 0.0023 | 0.0031 | 0.0037 | 0.0043 |
Mitochondria | 0.0107 | 0.0002 | 0.0068 | 0.0027 | 0.0102 | 0.0012 | 0.007 | 0.0002 | 0.0077 | 0.0046 | 0.0096 | 0.0002 | 0.0148 | 0.0033 | 0.0053 | 0.0186 | 0.0048 | 0.0008 |
None | 0.0103 | 0 | 0.0018 | 0.0013 | 0.0037 | 0.0001 | 0.0161 | 0 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0011 | 0.0001 | 0.0005 | 0.0006 | 0.0002 | 0 |
Nuclear Periphery | 0.0091 | 0.0003 | 0.0005 | 0.0103 | 0.0263 | 0.0024 | 0.0202 | 0.0002 | 0.0106 | 0.0001 | 0.0556 | 0.0029 | 0.0087 | 0.0025 | 0.0076 | 0.0029 | 0.0008 | 0.0005 |
Nucleolus | 0.1492 | 0.0621 | 0.0556 | 0.0664 | 0.4101 | 0.2383 | 0.0959 | 0.0486 | 0.0277 | 0.0692 | 0.3804 | 0.2013 | 0.126 | 0.0556 | 0.0631 | 0.1158 | 0.4397 | 0.2192 |
Nucleus | 0.7259 | 0.9372 | 0.8833 | 0.8514 | 0.4245 | 0.7548 | 0.7745 | 0.9509 | 0.9302 | 0.9253 | 0.492 | 0.7835 | 0.7677 | 0.9268 | 0.8868 | 0.7726 | 0.5012 | 0.7691 |
Peroxisomes | 0.0049 | 0 | 0.0053 | 0.0024 | 0.0018 | 0 | 0.0021 | 0 | 0.0004 | 0 | 0.0006 | 0 | 0.0041 | 0.0001 | 0.0004 | 0.002 | 0.0105 | 0.0008 |
Punctate Nuclear | 0.0137 | 0.0001 | 0.0036 | 0.0009 | 0.0216 | 0.0002 | 0.0354 | 0 | 0.0006 | 0 | 0.0108 | 0.0105 | 0.0335 | 0.0003 | 0.0168 | 0.0589 | 0.0013 | 0.0006 |
Vacuole | 0.0023 | 0 | 0.0004 | 0.0003 | 0.004 | 0.0004 | 0.0017 | 0 | 0.0005 | 0.0001 | 0.0007 | 0.0001 | 0.0007 | 0.0006 | 0.0005 | 0.0003 | 0.0003 | 0.0001 |
Vacuole Periphery | 0.0017 | 0 | 0.0004 | 0.0004 | 0.0043 | 0.0007 | 0.002 | 0 | 0.0013 | 0.0005 | 0.0038 | 0 | 0.0015 | 0.0008 | 0.001 | 0.0005 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 78.8146 | 76.2416 | 63.6464 | 63.5903 | 64.1751 | 62.4079 | 75.8833 | 78.0436 | 67.6544 | 67.5287 |
Translational Efficiency | 1.3711 | 1.6409 | 1.3413 | 1.3479 | 1.365 | 1.3648 | 1.5589 | 1.2182 | 1.2343 | 1.2716 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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