Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; non-essential gene; interacts with the DNA helicase Hpr5p; YGL101W has a paralog, YBR242W, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 5 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 1 | 1 | 4 | – | 4 | 3 | 2 | 4 | 0 | 0 | 2 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 285 | 346 | 140 | 109 | 180 | 159 | 210 | – | 139 | 171 | 125 | 128 | 194 | 280 | 285 | 82 | 97 | 77 | 277 | 331 | 136 | 159 | 255 | 212 |
Endoplasmic Reticulum | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 3 | 2 | 4 | 1 | 0 | 0 | 4 | 0 | 1 | 2 | 2 | 1 | 0 | 2 | 4 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 4 | 24 |
Golgi | 1 | 2 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 1 | 1 | 0 | 2 | 6 |
Mitochondria | 1 | 2 | 0 | 0 | 0 | 6 | 4 | – | 16 | 14 | 17 | 24 | 0 | 1 | 0 | 3 | 2 | 1 | 0 | 6 | 1 | 0 | 1 | 0 |
Nucleus | 2 | 3 | 1 | 2 | 2 | 2 | 5 | – | 4 | 9 | 9 | 5 | 2 | 1 | 0 | 2 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Vac/Vac Membrane | 6 | 2 | 4 | 16 | 36 | 44 | 59 | – | 21 | 29 | 23 | 20 | 0 | 2 | 3 | 2 | 1 | 2 | 1 | 5 | 0 | 0 | 1 | 3 |
Unique Cell Count | 291 | 351 | 144 | 119 | 208 | 202 | 270 | 172 | 215 | 167 | 172 | 196 | 282 | 287 | 86 | 99 | 80 | 293 | 358 | 146 | 164 | 277 | 268 | |
Labelled Cell Count | 298 | 356 | 146 | 128 | 219 | 213 | 283 | 189 | 229 | 180 | 187 | 198 | 284 | 290 | 99 | 105 | 86 | 293 | 358 | 146 | 164 | 277 | 268 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.1 | 13.0 | 11.6 | 11.4 | 12.4 | 8.2 | 10.4 | – | 8.7 | 9.2 | 8.3 | 7.8 | 13.7 | 11.2 | 13.6 | 17.4 | 17.9 | 18.5 | 12.0 | 12.3 | 12.5 |
Std Deviation (1e-4) | 2.2 | 2.4 | 1.9 | 1.7 | 1.9 | 2.0 | 2.0 | – | 1.7 | 1.5 | 1.2 | 1.5 | 2.1 | 1.9 | 2.0 | 4.3 | 4.5 | 3.8 | 2.1 | 1.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.11 | -0.5 | -0.15 | – | -0.4 | -0.32 | -0.48 | -0.57 | 0.24 | -0.05 | 0.24 | 0.59 | 0.63 | 0.68 | 0.05 | 0.09 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.0 | -3.4 | -5.0 | -5.2 | – | -4.5 | -4.8 | -5.5 | -5.6 | 1.2 | 1.7 | 1.7 | -0.8 | 0.4 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 3.8 | 3.1 | 3.9 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.2 | 4.2 | 5.0 | 5.1 | – | 3.1 | 3.4 | 3.4 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.4886 | 6.5654 | 5.5393 | 5.2348 | 4.3275 | 6.167 | 7.4218 | 8.9508 | 8.6546 | 8.828 | 8.902 | 9.1606 | 10.0623 | 11.5752 | 10.9505 | 10.7664 | 11.0225 | 10.9015 |
Actin | 0.0216 | 0.0033 | 0.0053 | 0.012 | 0.0032 | 0.0031 | 0.0121 | 0.0011 | 0.0169 | 0.0498 | 0.002 | 0.0028 | 0.0005 | 0.0006 | 0.0005 | 0.0009 | 0.0003 | 0.0004 |
Bud | 0.0017 | 0.0002 | 0.0006 | 0.0063 | 0.0017 | 0.0001 | 0.0006 | 0.0001 | 0.0012 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0005 | 0.0002 | 0.0004 | 0.0012 | 0.0002 | 0.0009 | 0.0021 | 0.0002 | 0.0015 | 0.001 | 0.0008 | 0.001 | 0.0002 | 0.0003 | 0.0006 | 0.0005 | 0.0003 | 0.0007 |
Bud Periphery | 0.0007 | 0.0001 | 0.0003 | 0.0083 | 0.002 | 0.0001 | 0.0006 | 0.0001 | 0.0011 | 0.0005 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0 |
Bud Site | 0.0024 | 0.0005 | 0.0014 | 0.0329 | 0.0012 | 0.0001 | 0.0018 | 0.0005 | 0.0051 | 0.0017 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8224 | 0.9747 | 0.9424 | 0.8973 | 0.7941 | 0.9471 | 0.8624 | 0.9753 | 0.9402 | 0.9101 | 0.9347 | 0.9663 | 0.972 | 0.9717 | 0.9842 | 0.9761 | 0.9894 | 0.9713 |
Cytoplasmic Foci | 0.0561 | 0.004 | 0.007 | 0.0089 | 0.0072 | 0.0069 | 0.0304 | 0.0032 | 0.0084 | 0.003 | 0.0061 | 0.0058 | 0.0015 | 0.0031 | 0.0022 | 0.0021 | 0.0015 | 0.0023 |
Eisosomes | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0032 | 0.0028 | 0.0039 | 0.0011 | 0.0007 | 0.001 | 0.0097 | 0.0018 | 0.0014 | 0.0015 | 0.0046 | 0.0023 | 0.0032 | 0.0014 | 0.0019 | 0.0018 | 0.0007 | 0.0054 |
Endosome | 0.0218 | 0.0031 | 0.0105 | 0.0026 | 0.0253 | 0.0023 | 0.0318 | 0.0022 | 0.0025 | 0.0034 | 0.018 | 0.0027 | 0.0006 | 0.0031 | 0.0008 | 0.0008 | 0.0004 | 0.0015 |
Golgi | 0.0049 | 0.0051 | 0.0033 | 0.0017 | 0.0256 | 0.0037 | 0.0072 | 0.0026 | 0.0013 | 0.0063 | 0.0007 | 0.0003 | 0 | 0.0016 | 0.0001 | 0.0001 | 0 | 0.0001 |
Lipid Particles | 0.0075 | 0.0001 | 0.0041 | 0.001 | 0.0005 | 0.0083 | 0.0022 | 0.0006 | 0.0006 | 0.0011 | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0044 | 0.0013 | 0.0027 | 0.0095 | 0.1283 | 0.0023 | 0.0018 | 0.0032 | 0.0009 | 0.0029 | 0.0003 | 0.0002 | 0.0001 | 0.0087 | 0.0001 | 0.0001 | 0.0001 | 0.0008 |
None | 0.0094 | 0.0008 | 0.0035 | 0.0022 | 0.0006 | 0.001 | 0.011 | 0.0021 | 0.0029 | 0.0016 | 0.0053 | 0.0018 | 0.0078 | 0.0016 | 0.0013 | 0.0022 | 0.0011 | 0.0025 |
Nuclear Periphery | 0.0025 | 0.0006 | 0.0025 | 0.0013 | 0.0005 | 0.0009 | 0.0046 | 0.0012 | 0.0015 | 0.0014 | 0.009 | 0.0017 | 0.002 | 0.0009 | 0.0011 | 0.0016 | 0.0006 | 0.0016 |
Nucleolus | 0.0004 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0055 | 0.0017 | 0.0065 | 0.0065 | 0.0048 | 0.0069 | 0.0114 | 0.0042 | 0.0087 | 0.0112 | 0.0105 | 0.0136 | 0.0109 | 0.0042 | 0.0061 | 0.0127 | 0.0049 | 0.012 |
Peroxisomes | 0.0183 | 0.0011 | 0.0014 | 0.0036 | 0.0011 | 0.0144 | 0.0035 | 0.0005 | 0.0038 | 0.0026 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 |
Punctate Nuclear | 0.0132 | 0.0001 | 0.0013 | 0.0011 | 0.0001 | 0.0005 | 0.0038 | 0.0006 | 0.0012 | 0.0006 | 0.0055 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0006 | 0.0002 | 0.0007 |
Vacuole | 0.0027 | 0.0002 | 0.0021 | 0.0013 | 0.0014 | 0.0002 | 0.0018 | 0.0003 | 0.0005 | 0.0003 | 0.0007 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0008 | 0.0003 | 0.0014 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.4334 | 45.6656 | 25.9791 | 18.1282 | 30.9083 | 15.3166 | 40.7856 | 26.4888 | 21.9604 | 25.4385 |
Translational Efficiency | 2.1796 | 1.5292 | 1.2778 | 1.4111 | 1.4522 | 2.345 | 1.5204 | 1.6807 | 1.7858 | 1.9248 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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