Standard name
Human Ortholog
Description Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases; involved in tRNA delivery, stimulating catalysis, and ensuring localization; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress; methionyl-tRNA synthetase is Mes1p; glutamyl-tRNA synthetase is Gus1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.09 0.07 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.11 0.06 0.07 0.09 0.08 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.97 0.91 0.85 0.66 0.74 0.72 0.71 0.73 0.66 0.96 0.99 0.98 0.98 0.96 0.96 0.95 0.94 0.93 0.9 0.87 0.89
Endoplasmic Reticulum 0 0 0 0 0.09 0.11 0.24 0.2 0.2 0.17 0.13 0.19 0 0 0 0 0 0 0 0 0 0.06 0.07 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 4 18 13 22 33 19 34 0 0 0 0 0 0 0 0 0 0 2 1
Bud 0 1 0 0 0 0 1 1 1 1 2 2 0 0 0 0 0 0 0 2 2 2 5 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 2 7 13 21 17 49 29 23 35 21 28 5 6 11 2 4 5 0 0 2 0 0 1
Cytoplasm 347 358 412 372 489 329 307 351 246 276 189 190 425 530 563 65 99 108 321 348 412 382 469 601
Endoplasmic Reticulum 2 5 7 12 47 43 113 96 68 65 34 55 12 6 9 2 4 5 10 4 15 26 39 34
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Golgi 0 0 0 0 0 8 17 7 12 12 4 15 1 4 1 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 6 4 13 7 17 8 0 0 0 0 1 2 0 1 1 1 1 2
Nucleus 0 0 0 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 1 1 1 1 3 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 2 0 0 3 3 3 0 5 4 5 2 4 2 0 1 0 0 5 4 1 4 6
Unique Cell Count 350 365 423 383 539 387 463 472 344 388 258 288 443 538 572 66 103 113 340 371 445 424 537 677
Labelled Cell Count 350 368 428 397 559 404 514 504 387 434 290 337 447 550 587 69 109 120 340 371 445 424 537 677


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 65.5 84.3 61.7 58.1 51.6 45.4 41.5 40.3 36.8 35.9 33.3 34.2 69.0 70.7 72.1 90.6 91.3 87.9 72.2 75.0 79.3
Std Deviation (1e-4) 12.0 18.7 12.2 9.7 11.0 10.4 9.0 9.7 8.9 8.2 8.3 9.5 17.2 17.3 16.1 33.2 31.2 26.4 16.1 19.8 21.4
Intensity Change (Log2) -0.09 -0.26 -0.44 -0.57 -0.62 -0.75 -0.78 -0.89 -0.85 0.16 0.2 0.22 0.55 0.56 0.51 0.22 0.28 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 4.1 3.4 5.3 6.1 5.7 7.2 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.6 2.1 2.3 5.5 3.4 3.6 4.7 4.1 4.9 -0.7 -0.7 0.3 0 0 0
Cytoplasm -0.2 -4.2 -6.3 -11.8 -9.7 -10.2 -10.4 -9.5 -11.4 -1.2 1.2 1.1 0.5 -0.7 -1.0
Endoplasmic Reticulum 1.4 4.7 5.6 9.9 8.7 8.4 7.6 6.1 8.1 1.1 -0.7 -0.1 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 4.0 0 3.9 3.6 0 4.7 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.0 0 5.3 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 49.2997 58.5061 55.7707 52.3236 56.1446 54.4446 48.2226 62.2591 56.3437 51.9583 49.9907 56.304 45.6609 69.0663 66.2322 49.7689 57.6166 59.9553
Actin 0.0005 0.0004 0.0007 0.0004 0.0003 0.0006 0.0072 0.0003 0.0034 0.0002 0.0011 0.0005 0.0092 0.0002 0.0009 0.0023 0.0044 0.0008
Bud 0.0006 0.0003 0.0004 0.0012 0.0002 0.0003 0.0008 0.0002 0.0009 0.0015 0.001 0.0004 0.0021 0.0002 0.0009 0.002 0.0032 0.0007
Bud Neck 0.0005 0.0003 0.0005 0.0005 0.0007 0.001 0.0007 0.0003 0.0032 0.0005 0.0007 0.001 0.0082 0.0003 0.0007 0.0014 0.0019 0.0018
Bud Periphery 0.0007 0.0003 0.0002 0.0025 0.0001 0.0002 0.0008 0.0001 0.0009 0.0024 0.0005 0.0003 0.0027 0.0001 0.0005 0.0019 0.0076 0.0018
Bud Site 0.0002 0.0004 0.0002 0.0001 0 0 0.0007 0.0003 0.0007 0.0004 0.0008 0.0001 0.0037 0.0002 0.0058 0.0023 0.0057 0.0001
Cell Periphery 0.0075 0.0036 0.0029 0.0025 0.0005 0.001 0.0073 0.0058 0.008 0.0085 0.0027 0.0018 0.0143 0.0098 0.0016 0.0022 0.0194 0.0044
Cytoplasm 0.8105 0.9536 0.9219 0.8425 0.9362 0.8929 0.7818 0.9315 0.8748 0.8249 0.8523 0.8573 0.7055 0.9377 0.937 0.7038 0.7546 0.8403
Cytoplasmic Foci 0.0024 0.0017 0.0028 0.0025 0.0046 0.0043 0.0055 0.0012 0.0053 0.0022 0.0072 0.0036 0.007 0.0008 0.0024 0.005 0.009 0.005
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0 0.0028 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0871 0.0109 0.0209 0.0255 0.0187 0.0702 0.067 0.0288 0.0214 0.0244 0.0039 0.1046 0.0536 0.0056 0.004 0.0093 0.0071 0.0261
Endosome 0.0121 0.0029 0.0068 0.0145 0.0104 0.01 0.0136 0.0016 0.0084 0.0063 0.0119 0.0092 0.0154 0.0014 0.0046 0.0599 0.0154 0.031
Golgi 0.0023 0.0009 0.0017 0.0017 0.002 0.0028 0.0015 0.0005 0.002 0.0002 0.0009 0.0018 0.0032 0.0003 0.0009 0.004 0.0024 0.0118
Lipid Particles 0.0006 0.0026 0.0007 0.0008 0.0002 0.0006 0.0048 0.0019 0.0033 0.0013 0.0019 0.0005 0.0046 0.0007 0.0003 0.0045 0.0051 0.001
Mitochondria 0.0037 0.0009 0.0006 0.0021 0.0005 0.0018 0.0059 0.0001 0.0012 0.0016 0.0023 0.0013 0.0115 0.0001 0.0004 0.0063 0.021 0.0016
None 0.0008 0.0002 0.0004 0.0002 0.0001 0.0003 0.0025 0.0001 0.0032 0.0013 0.0063 0.0003 0.0073 0.0001 0.0003 0.0009 0.0045 0.0011
Nuclear Periphery 0.0105 0.0011 0.0022 0.0028 0.0013 0.002 0.0143 0.0019 0.0051 0.0056 0.0116 0.0034 0.0354 0.0013 0.0023 0.0195 0.0108 0.0119
Nucleolus 0.0001 0 0.0003 0.0001 0 0 0.003 0 0.0004 0.0005 0.004 0 0.0052 0.0001 0.0001 0.0018 0.0124 0.0003
Nucleus 0.0028 0.0009 0.0018 0.0016 0.0008 0.001 0.0146 0.001 0.0025 0.0052 0.0126 0.0013 0.0091 0.0013 0.002 0.0247 0.0252 0.0047
Peroxisomes 0 0 0 0 0 0 0.0017 0 0.0008 0.0001 0.0002 0 0.0005 0 0 0.0004 0.0009 0.0001
Punctate Nuclear 0.0001 0 0.0001 0 0 0.0001 0.0013 0 0.0039 0.0001 0.0116 0.0001 0.0043 0 0.0001 0.0009 0.0056 0.0002
Vacuole 0.0493 0.018 0.0311 0.0778 0.0191 0.0094 0.0575 0.0233 0.0467 0.101 0.0594 0.0106 0.082 0.0379 0.0327 0.118 0.0646 0.0459
Vacuole Periphery 0.0073 0.0012 0.0037 0.0208 0.0041 0.0014 0.0072 0.0008 0.004 0.0119 0.0071 0.0019 0.0123 0.002 0.0025 0.029 0.019 0.0096

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 416.5815 443.0408 412.2385 403.7557 417.42 402.7 363.7455 347.5737 355.0945 347.1512
Translational Efficiency 1.9054 1.8639 1.9715 2.1888 2.1575 1.8485 1.7963 2.044 1.8502 1.9215

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases; involved in tRNA delivery, stimulating catalysis, and ensuring localization; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress; methionyl-tRNA synthetase is Mes1p; glutamyl-tRNA synthetase is Gus1p
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Arc1

Arc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available