ORF | |
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Human Ortholog | |
Description | Protein of unknown function, predicted to be palmitoylated; SWAT-GFP, seamless-GFP and mCherry C-terminal fusion proteins localize to the cytosol, while N-terminal GFP fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 29 | 61 | 39 | 15 | 20 | 20 | – | 24 | 12 | 17 | 15 | 20 | 28 | 41 | 24 | 21 | 16 | 11 | 2 | 6 | 2 | 4 | 13 | 9 |
Bud | 2 | 4 | 13 | 3 | 8 | 2 | – | 7 | 7 | 5 | 10 | 5 | 7 | 7 | 1 | 1 | 3 | 0 | 0 | 3 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 9 | 6 | 3 | 5 | 8 |
Bud Site | 1 | 0 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 91 | 241 | 113 | 94 | 80 | 126 | – | 128 | 90 | 95 | 110 | 135 | 168 | 200 | 217 | 203 | 130 | 93 | 109 | 297 | 156 | 169 | 286 | 276 |
Cytoplasm | 43 | 74 | 41 | 34 | 51 | 64 | – | 149 | 78 | 79 | 102 | 102 | 72 | 99 | 118 | 45 | 33 | 36 | 8 | 12 | 1 | 4 | 13 | 13 |
Endoplasmic Reticulum | 3 | 2 | 2 | 2 | 2 | 10 | – | 7 | 8 | 5 | 5 | 8 | 4 | 1 | 5 | 22 | 7 | 12 | 1 | 4 | 1 | 1 | 8 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 3 | 1 | 0 | 2 | 1 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 4 | 2 | 0 | 2 | 0 | 1 | 1 | 0 |
Mitochondria | 9 | 83 | 78 | 107 | 68 | 118 | – | 230 | 150 | 155 | 197 | 257 | 32 | 68 | 46 | 26 | 22 | 16 | 2 | 6 | 4 | 4 | 3 | 5 |
Nucleus | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2 | 0 | 2 | 1 | 4 | – | 2 | 3 | 1 | 1 | 0 | 11 | 17 | 23 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 2 | 2 | 4 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 0 | 0 | – | 9 | 1 | 2 | 4 | 6 | 1 | 2 | 2 | 6 | 3 | 2 | 0 | 4 | 1 | 1 | 2 | 2 |
Unique Cell Count | 119 | 303 | 164 | 147 | 151 | 214 | 370 | 223 | 224 | 287 | 358 | 218 | 275 | 295 | 248 | 164 | 132 | 137 | 355 | 179 | 193 | 344 | 336 | |
Labelled Cell Count | 178 | 471 | 288 | 261 | 231 | 344 | 558 | 349 | 360 | 448 | 539 | 326 | 441 | 440 | 336 | 220 | 179 | 137 | 355 | 179 | 193 | 344 | 336 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 7.2 | 5.2 | 5.9 | 6.4 | 5.3 | – | 5.4 | 5.1 | 5.0 | 4.9 | 4.9 | 7.5 | 7.6 | 7.5 | 9.8 | 9.5 | 9.2 | 7.7 | 8.0 | 8.2 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.1 | 1.5 | 1.5 | 0.8 | – | 1.1 | 1.4 | 1.0 | 1.2 | 1.2 | 1.6 | 1.9 | 1.9 | 2.0 | 1.8 | 1.9 | 1.4 | 2.1 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.19 | 0.31 | 0.03 | – | 0.06 | -0.04 | -0.05 | -0.08 | -0.09 | 0.53 | 0.55 | 0.53 | 0.91 | 0.86 | 0.82 | 0.57 | 0.62 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.2 | -2.4 | -3.8 | – | -5.7 | -5.3 | -4.5 | -5.8 | 0 | -2.8 | -2.3 | -4.7 | -4.3 | -3.4 | -3.5 |
Bud | -2.3 | -0.9 | -3.5 | – | -3.4 | -2.1 | -2.6 | -2.1 | 0 | -2.0 | -2.6 | -4.5 | -4.1 | -2.6 | -3.3 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.9 | -2.9 | -2.0 | – | -7.3 | -5.6 | -5.2 | -6.2 | 0 | 1.8 | 0.9 | 1.1 | 3.0 | 2.1 | 0.3 |
Cytoplasm | -0.4 | 1.7 | 1.1 | – | 3.4 | 2.1 | 2.2 | 2.3 | 0.8 | 1.7 | 2.4 | 3.2 | -1.7 | -1.1 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 1.9 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 3.2 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.5 | -0.4 | 1.5 | – | 3.2 | 3.9 | 4.3 | 4.4 | 0 | -7.0 | -4.9 | -7.4 | -8.5 | -6.7 | -6.5 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.7 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.601 | 2.5362 | 2.1989 | 1.9228 | 1.6144 | 2.1725 | 1.8945 | 2.3733 | 2.2214 | 1.9073 | 1.3124 | 2.1288 | 3.5218 | 4.0714 | 3.7965 | 3.5088 | 4.8812 | 3.5764 |
Actin | 0.0498 | 0.0015 | 0.0223 | 0.0003 | 0.0046 | 0.0021 | 0.05 | 0.0022 | 0.022 | 0.0372 | 0.0079 | 0.0079 | 0.0459 | 0.0011 | 0.0027 | 0.0132 | 0.0062 | 0.0157 |
Bud | 0.0011 | 0.0001 | 0.0006 | 0.0003 | 0.0006 | 0.0005 | 0.0045 | 0.0003 | 0.0048 | 0.0013 | 0.0007 | 0.0007 | 0.0023 | 0.0004 | 0.0009 | 0.0004 | 0.0021 | 0.0012 |
Bud Neck | 0.0084 | 0.005 | 0.0065 | 0.0572 | 0.0077 | 0.0132 | 0.0092 | 0.0038 | 0.0084 | 0.0124 | 0.0203 | 0.0149 | 0.0161 | 0.0033 | 0.0099 | 0.0171 | 0.0034 | 0.0134 |
Bud Periphery | 0.0063 | 0.0009 | 0.0042 | 0.0029 | 0.0066 | 0.0081 | 0.0242 | 0.0019 | 0.0081 | 0.0102 | 0.0043 | 0.0095 | 0.018 | 0.0025 | 0.0046 | 0.0026 | 0.0102 | 0.0085 |
Bud Site | 0.0237 | 0.0615 | 0.0298 | 0.0119 | 0.0083 | 0.0022 | 0.0212 | 0.1069 | 0.0808 | 0.0153 | 0.0359 | 0.0043 | 0.0281 | 0.1089 | 0.0571 | 0.0193 | 0.0048 | 0.0042 |
Cell Periphery | 0.5446 | 0.6683 | 0.6546 | 0.701 | 0.7087 | 0.7832 | 0.5048 | 0.5699 | 0.5664 | 0.6522 | 0.7193 | 0.6925 | 0.5877 | 0.552 | 0.6046 | 0.6052 | 0.6661 | 0.6617 |
Cytoplasm | 0.1696 | 0.241 | 0.2079 | 0.2147 | 0.1506 | 0.1703 | 0.2432 | 0.2987 | 0.2433 | 0.1744 | 0.137 | 0.2285 | 0.1493 | 0.3174 | 0.2544 | 0.2267 | 0.1229 | 0.2343 |
Cytoplasmic Foci | 0.0304 | 0.0008 | 0.0028 | 0.002 | 0.0061 | 0.0009 | 0.0123 | 0.0004 | 0.0079 | 0.0047 | 0.0005 | 0.0016 | 0.043 | 0.0004 | 0.0057 | 0.0096 | 0.0203 | 0.0019 |
Eisosomes | 0.0164 | 0.002 | 0.0048 | 0.0002 | 0.0004 | 0.0006 | 0.0034 | 0.0027 | 0.0053 | 0.0053 | 0.0011 | 0.0015 | 0.0025 | 0.0007 | 0.0023 | 0.0001 | 0.0008 | 0.0002 |
Endoplasmic Reticulum | 0.0036 | 0.0017 | 0.0047 | 0.0023 | 0.0057 | 0.0022 | 0.0138 | 0.0013 | 0.0027 | 0.0048 | 0.0014 | 0.0034 | 0.0037 | 0.001 | 0.002 | 0.0016 | 0.0021 | 0.0023 |
Endosome | 0.0152 | 0 | 0.0107 | 0.0005 | 0.0366 | 0.0001 | 0.0198 | 0 | 0.0115 | 0.0158 | 0.0001 | 0.0034 | 0.0184 | 0 | 0.0012 | 0.0008 | 0.072 | 0.0118 |
Golgi | 0.0086 | 0.0001 | 0.0178 | 0.0004 | 0.0025 | 0.0002 | 0.0057 | 0.0001 | 0.0047 | 0.0157 | 0.0003 | 0.0012 | 0.009 | 0.0001 | 0.0006 | 0.0027 | 0.039 | 0.0138 |
Lipid Particles | 0.0153 | 0.0004 | 0.0029 | 0.0004 | 0.0095 | 0.001 | 0.0074 | 0.0002 | 0.0038 | 0.0036 | 0.0006 | 0.0024 | 0.0212 | 0.0002 | 0.0009 | 0.0021 | 0.0235 | 0.0067 |
Mitochondria | 0.0149 | 0 | 0.0074 | 0.0001 | 0.001 | 0.0001 | 0.003 | 0 | 0.0013 | 0.0346 | 0 | 0.0004 | 0.0039 | 0 | 0.0001 | 0.0014 | 0.0059 | 0.0137 |
None | 0.0516 | 0.0152 | 0.0161 | 0.0018 | 0.0082 | 0.012 | 0.0275 | 0.0099 | 0.014 | 0.0042 | 0.0681 | 0.0162 | 0.0083 | 0.0099 | 0.0459 | 0.0004 | 0.009 | 0.0031 |
Nuclear Periphery | 0.0021 | 0.0001 | 0.0023 | 0.0001 | 0.0159 | 0.0001 | 0.0155 | 0.0001 | 0.001 | 0.0006 | 0.0001 | 0.0037 | 0.0069 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0004 |
Nucleolus | 0.0009 | 0.0001 | 0.0002 | 0.0012 | 0.0011 | 0.0002 | 0.0016 | 0.0001 | 0.0013 | 0.0003 | 0.0002 | 0.0004 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 |
Nucleus | 0.001 | 0.0001 | 0.0005 | 0.0004 | 0.004 | 0.0002 | 0.0083 | 0.0002 | 0.0035 | 0.0004 | 0.0002 | 0.0008 | 0.0017 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0004 |
Peroxisomes | 0.024 | 0.0001 | 0.0008 | 0.0003 | 0.0009 | 0 | 0.008 | 0 | 0.003 | 0.0028 | 0 | 0.0003 | 0.0177 | 0 | 0.0014 | 0.094 | 0.0031 | 0.0012 |
Punctate Nuclear | 0.006 | 0 | 0.0003 | 0.0001 | 0.0108 | 0.0001 | 0.0075 | 0 | 0.001 | 0.0005 | 0.0001 | 0.002 | 0.0076 | 0 | 0.0015 | 0.0007 | 0.0004 | 0.0004 |
Vacuole | 0.0059 | 0.0009 | 0.0024 | 0.002 | 0.0091 | 0.0029 | 0.0077 | 0.0012 | 0.0047 | 0.0032 | 0.0018 | 0.0043 | 0.0067 | 0.0015 | 0.0035 | 0.0019 | 0.0058 | 0.0045 |
Vacuole Periphery | 0.0006 | 0 | 0.0004 | 0 | 0.0009 | 0 | 0.0013 | 0 | 0.0004 | 0.0007 | 0 | 0.0002 | 0.0008 | 0 | 0 | 0 | 0.0011 | 0.0005 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.7383 | 6.9956 | 15.5731 | 15.5844 | 13.5282 | 23.4637 | 14.1464 | 15.9132 | 19.5204 | 13.8682 |
Translational Efficiency | 1.4611 | 2.1618 | 1.3101 | 1.4016 | 1.5663 | 0.7548 | 1.1623 | 1.9978 | 1.4121 | 1.3933 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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