ORF
Human Ortholog
Description Protein of unknown function, predicted to be palmitoylated; SWAT-GFP, seamless-GFP and mCherry C-terminal fusion proteins localize to the cytosol, while N-terminal GFP fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.24 0.2 0.24 0.1 0.13 0.09 0.06 0.05 0.08 0.05 0.06 0.13 0.15 0.08 0.08 0.1 0.08 0 0 0 0 0 0
Bud 0 0 0.08 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.76 0.8 0.69 0.64 0.53 0.59 0.35 0.4 0.42 0.38 0.38 0.77 0.73 0.74 0.82 0.79 0.7 0.8 0.84 0.87 0.88 0.83 0.82
Cytoplasm 0.36 0.24 0.25 0.23 0.34 0.3 0.4 0.35 0.35 0.36 0.28 0.33 0.36 0.4 0.18 0.2 0.27 0.06 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.09 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.27 0.48 0.73 0.45 0.55 0.62 0.67 0.69 0.69 0.72 0.15 0.25 0.16 0.1 0.13 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 29 61 39 15 20 20 24 12 17 15 20 28 41 24 21 16 11 2 6 2 4 13 9
Bud 2 4 13 3 8 2 7 7 5 10 5 7 7 1 1 3 0 0 3 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 9 6 3 5 8
Bud Site 1 0 1 1 1 0 0 0 0 0 1 0 0 0 0 0 1
Cell Periphery 91 241 113 94 80 126 128 90 95 110 135 168 200 217 203 130 93 109 297 156 169 286 276
Cytoplasm 43 74 41 34 51 64 149 78 79 102 102 72 99 118 45 33 36 8 12 1 4 13 13
Endoplasmic Reticulum 3 2 2 2 2 10 7 8 5 5 8 4 1 5 22 7 12 1 4 1 1 8 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 3 1 0 2 1
Golgi 0 1 0 0 0 0 0 0 0 0 1 0 1 1 2 4 2 0 2 0 1 1 0
Mitochondria 9 83 78 107 68 118 230 150 155 197 257 32 68 46 26 22 16 2 6 4 4 3 5
Nucleus 0 1 0 1 0 0 0 0 0 1 1 0 1 0 4 2 1 0 0 0 0 0 0
Nuclear Periphery 0 1 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 1 1 1 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 2 0 2 1 4 2 3 1 1 0 11 17 23 5 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 1 0 0 1 0 0 0 2 2 4 1 1 0 3 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 0 9 1 2 4 6 1 2 2 6 3 2 0 4 1 1 2 2
Unique Cell Count 119 303 164 147 151 214 370 223 224 287 358 218 275 295 248 164 132 137 355 179 193 344 336
Labelled Cell Count 178 471 288 261 231 344 558 349 360 448 539 326 441 440 336 220 179 137 355 179 193 344 336


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 7.2 5.2 5.9 6.4 5.3 5.4 5.1 5.0 4.9 4.9 7.5 7.6 7.5 9.8 9.5 9.2 7.7 8.0 8.2
Std Deviation (1e-4) 0.7 0.8 1.1 1.5 1.5 0.8 1.1 1.4 1.0 1.2 1.2 1.6 1.9 1.9 2.0 1.8 1.9 1.4 2.1 2.1
Intensity Change (Log2) 0.19 0.31 0.03 0.06 -0.04 -0.05 -0.08 -0.09 0.53 0.55 0.53 0.91 0.86 0.82 0.57 0.62 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.2 -2.4 -3.8 -5.7 -5.3 -4.5 -5.8 0 -2.8 -2.3 -4.7 -4.3 -3.4 -3.5
Bud -2.3 -0.9 -3.5 -3.4 -2.1 -2.6 -2.1 0 -2.0 -2.6 -4.5 -4.1 -2.6 -3.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -2.9 -2.0 -7.3 -5.6 -5.2 -6.2 0 1.8 0.9 1.1 3.0 2.1 0.3
Cytoplasm -0.4 1.7 1.1 3.4 2.1 2.2 2.3 0.8 1.7 2.4 3.2 -1.7 -1.1 0.4
Endoplasmic Reticulum 0 0 1.9 0 0 0 0 0 0 0 0 3.2 0 3.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.5 -0.4 1.5 3.2 3.9 4.3 4.4 0 -7.0 -4.9 -7.4 -8.5 -6.7 -6.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 3.2 3.7 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.601 2.5362 2.1989 1.9228 1.6144 2.1725 1.8945 2.3733 2.2214 1.9073 1.3124 2.1288 3.5218 4.0714 3.7965 3.5088 4.8812 3.5764
Actin 0.0498 0.0015 0.0223 0.0003 0.0046 0.0021 0.05 0.0022 0.022 0.0372 0.0079 0.0079 0.0459 0.0011 0.0027 0.0132 0.0062 0.0157
Bud 0.0011 0.0001 0.0006 0.0003 0.0006 0.0005 0.0045 0.0003 0.0048 0.0013 0.0007 0.0007 0.0023 0.0004 0.0009 0.0004 0.0021 0.0012
Bud Neck 0.0084 0.005 0.0065 0.0572 0.0077 0.0132 0.0092 0.0038 0.0084 0.0124 0.0203 0.0149 0.0161 0.0033 0.0099 0.0171 0.0034 0.0134
Bud Periphery 0.0063 0.0009 0.0042 0.0029 0.0066 0.0081 0.0242 0.0019 0.0081 0.0102 0.0043 0.0095 0.018 0.0025 0.0046 0.0026 0.0102 0.0085
Bud Site 0.0237 0.0615 0.0298 0.0119 0.0083 0.0022 0.0212 0.1069 0.0808 0.0153 0.0359 0.0043 0.0281 0.1089 0.0571 0.0193 0.0048 0.0042
Cell Periphery 0.5446 0.6683 0.6546 0.701 0.7087 0.7832 0.5048 0.5699 0.5664 0.6522 0.7193 0.6925 0.5877 0.552 0.6046 0.6052 0.6661 0.6617
Cytoplasm 0.1696 0.241 0.2079 0.2147 0.1506 0.1703 0.2432 0.2987 0.2433 0.1744 0.137 0.2285 0.1493 0.3174 0.2544 0.2267 0.1229 0.2343
Cytoplasmic Foci 0.0304 0.0008 0.0028 0.002 0.0061 0.0009 0.0123 0.0004 0.0079 0.0047 0.0005 0.0016 0.043 0.0004 0.0057 0.0096 0.0203 0.0019
Eisosomes 0.0164 0.002 0.0048 0.0002 0.0004 0.0006 0.0034 0.0027 0.0053 0.0053 0.0011 0.0015 0.0025 0.0007 0.0023 0.0001 0.0008 0.0002
Endoplasmic Reticulum 0.0036 0.0017 0.0047 0.0023 0.0057 0.0022 0.0138 0.0013 0.0027 0.0048 0.0014 0.0034 0.0037 0.001 0.002 0.0016 0.0021 0.0023
Endosome 0.0152 0 0.0107 0.0005 0.0366 0.0001 0.0198 0 0.0115 0.0158 0.0001 0.0034 0.0184 0 0.0012 0.0008 0.072 0.0118
Golgi 0.0086 0.0001 0.0178 0.0004 0.0025 0.0002 0.0057 0.0001 0.0047 0.0157 0.0003 0.0012 0.009 0.0001 0.0006 0.0027 0.039 0.0138
Lipid Particles 0.0153 0.0004 0.0029 0.0004 0.0095 0.001 0.0074 0.0002 0.0038 0.0036 0.0006 0.0024 0.0212 0.0002 0.0009 0.0021 0.0235 0.0067
Mitochondria 0.0149 0 0.0074 0.0001 0.001 0.0001 0.003 0 0.0013 0.0346 0 0.0004 0.0039 0 0.0001 0.0014 0.0059 0.0137
None 0.0516 0.0152 0.0161 0.0018 0.0082 0.012 0.0275 0.0099 0.014 0.0042 0.0681 0.0162 0.0083 0.0099 0.0459 0.0004 0.009 0.0031
Nuclear Periphery 0.0021 0.0001 0.0023 0.0001 0.0159 0.0001 0.0155 0.0001 0.001 0.0006 0.0001 0.0037 0.0069 0.0001 0.0002 0.0001 0.0005 0.0004
Nucleolus 0.0009 0.0001 0.0002 0.0012 0.0011 0.0002 0.0016 0.0001 0.0013 0.0003 0.0002 0.0004 0.0008 0.0001 0.0002 0 0.0002 0.0002
Nucleus 0.001 0.0001 0.0005 0.0004 0.004 0.0002 0.0083 0.0002 0.0035 0.0004 0.0002 0.0008 0.0017 0.0002 0.0004 0.0002 0.0003 0.0004
Peroxisomes 0.024 0.0001 0.0008 0.0003 0.0009 0 0.008 0 0.003 0.0028 0 0.0003 0.0177 0 0.0014 0.094 0.0031 0.0012
Punctate Nuclear 0.006 0 0.0003 0.0001 0.0108 0.0001 0.0075 0 0.001 0.0005 0.0001 0.002 0.0076 0 0.0015 0.0007 0.0004 0.0004
Vacuole 0.0059 0.0009 0.0024 0.002 0.0091 0.0029 0.0077 0.0012 0.0047 0.0032 0.0018 0.0043 0.0067 0.0015 0.0035 0.0019 0.0058 0.0045
Vacuole Periphery 0.0006 0 0.0004 0 0.0009 0 0.0013 0 0.0004 0.0007 0 0.0002 0.0008 0 0 0 0.0011 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.7383 6.9956 15.5731 15.5844 13.5282 23.4637 14.1464 15.9132 19.5204 13.8682
Translational Efficiency 1.4611 2.1618 1.3101 1.4016 1.5663 0.7548 1.1623 1.9978 1.4121 1.3933

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
ORF
Human Ortholog
Description Protein of unknown function, predicted to be palmitoylated; SWAT-GFP, seamless-GFP and mCherry C-terminal fusion proteins localize to the cytosol, while N-terminal GFP fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cell periphery (57%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

YGL108C

YGL108C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ygl108c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available