Standard name
Human Ortholog
Description Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0.05 0 0 0.07 0 0.05 0 0.11 0.13 0.13 0 0 0 0 0 0
Cytoplasm 0.98 0.99 1.0 0.97 0.95 0.91 0.82 0.85 0.77 0.77 0.72 0.73 1.0 0.98 0.99 0.96 0.93 0.94 0.95 0.96 0.94 0.93 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.23 0.09 0.35 0.27 0.34 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 3 1 4 4 0 4 8 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 2 1 1 1 2 2 1 7 7 3 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 3 3 6 6 6 12 12 11 6 9 14 0 8 5 22 23 29 0 0 0 0 0 0
Cytoplasm 291 180 161 142 272 183 193 259 159 190 157 158 16 151 161 198 166 205 278 173 159 78 108 143
Endoplasmic Reticulum 3 3 0 0 5 5 4 15 1 5 7 6 0 0 3 3 5 4 6 2 2 0 2 1
Endosome 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1
Golgi 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0
Mitochondria 2 0 0 1 8 19 54 26 73 66 74 51 0 0 0 3 2 4 1 0 2 0 2 0
Nucleus 0 0 0 0 0 1 3 1 1 0 0 1 0 4 0 2 3 0 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 1 0 3 4 2 4 10 2 11 2 9 0 0 0 1 9 2 1 1 2 1 1 0
Unique Cell Count 297 182 161 147 287 202 234 304 207 248 218 215 16 154 163 207 178 218 292 181 169 84 116 152
Labelled Cell Count 303 189 165 156 299 221 273 328 258 285 256 249 16 163 169 229 211 247 292 181 169 84 116 152


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 13.6 9.6 9.6 9.6 8.3 7.5 8.2 6.9 7.2 6.8 7.4 8.8 11.1 10.9 14.4 15.7 15.2 9.4 10.5 12.1
Std Deviation (1e-4) 1.3 1.9 1.3 1.6 1.4 1.4 1.5 1.4 1.4 1.4 1.3 1.6 1.1 1.6 1.3 3.0 3.0 3.6 1.6 1.6 2.0
Intensity Change (Log2) – – – -0.0 0.01 -0.21 -0.35 -0.21 -0.47 -0.4 -0.49 -0.37 -0.13 0.22 0.19 0.59 0.71 0.67 -0.02 0.14 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.7 1.2 1.7 0 1.2 2.1 0 1.6 0 3.3 3.8 4.0
Cytoplasm -2.4 -3.0 -4.0 -5.6 -5.1 -6.6 -6.6 -7.3 -7.1 0 -1.8 -1.4 -2.7 -3.4 -3.2
Endoplasmic Reticulum 0 0 0 0 2.9 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.0 6.6 3.8 8.4 7.1 8.2 6.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7305 7.6145 7.3267 6.63 6.7464 6.8534 6.3724 9.157 7.8419 7.4655 6.5272 7.7342 7.7884 9.0176 8.6204 8.5024 8.7025 8.3637
Actin 0.02 0.002 0.0002 0.0118 0.0004 0.0001 0.0098 0.0001 0.0148 0.0001 0.0065 0.0013 0.0006 0.0005 0.0002 0.0002 0.0004 0.0001
Bud 0.0005 0.0008 0.0004 0.0044 0.0003 0.0001 0.0045 0.001 0.0052 0.0003 0.0143 0.0033 0.002 0.0048 0.0002 0.0012 0.0001 0.0001
Bud Neck 0.0007 0.0001 0.0008 0.0005 0.0012 0.0005 0.0014 0.0001 0.0006 0.0005 0.0007 0.0008 0.0002 0.0002 0.0006 0.0005 0.0003 0.0005
Bud Periphery 0.0005 0.0003 0.0001 0.0045 0.0001 0 0.002 0.0004 0.0023 0.0001 0.0116 0.0007 0.0008 0.0008 0.0001 0.0004 0 0.0001
Bud Site 0.0006 0.0026 0.0053 0.0041 0.0001 0 0.0042 0.0011 0.0138 0.0003 0.0027 0.0003 0.0013 0.002 0.0003 0.0004 0.0001 0
Cell Periphery 0.0003 0.0001 0.0001 0.0004 0 0 0.0007 0.0001 0.0002 0 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0 0
Cytoplasm 0.8492 0.9694 0.9332 0.8338 0.9486 0.9343 0.8532 0.9678 0.9103 0.9733 0.777 0.933 0.8595 0.9397 0.9418 0.8651 0.8976 0.9057
Cytoplasmic Foci 0.0124 0.0063 0.0115 0.0129 0.0165 0.0113 0.0204 0.0056 0.0109 0.0041 0.0252 0.0099 0.0085 0.0066 0.0069 0.0107 0.0133 0.0095
Eisosomes 0.0003 0 0 0.0003 0 0 0.0002 0 0.0001 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0162 0.0027 0.0053 0.0107 0.0012 0.0117 0.0142 0.0074 0.0032 0.0011 0.0038 0.009 0.0679 0.0177 0.0162 0.0294 0.0067 0.033
Endosome 0.0434 0.0087 0.0266 0.0506 0.019 0.0296 0.0374 0.0087 0.0175 0.0067 0.0746 0.0195 0.0255 0.0154 0.0199 0.0491 0.0711 0.0389
Golgi 0.0038 0.0005 0.0008 0.0026 0.001 0.0009 0.0079 0.0005 0.0017 0.0001 0.008 0.0033 0.0012 0.0013 0.0005 0.0015 0.0013 0.0014
Lipid Particles 0.002 0 0.0001 0.0021 0 0.0001 0.0025 0 0.0022 0 0.0124 0.0017 0.0003 0.0001 0.0001 0.0001 0.0001 0
Mitochondria 0.0061 0.0002 0.0004 0.014 0.0005 0.0006 0.0045 0.0002 0.0059 0.0001 0.0025 0.0024 0.0049 0.0007 0.0002 0.0014 0.0003 0.0014
None 0.0195 0.0018 0.0031 0.0042 0.0027 0.0032 0.0106 0.0025 0.0032 0.0031 0.0025 0.0017 0.0086 0.0013 0.0019 0.001 0.0005 0.0014
Nuclear Periphery 0.0048 0.0006 0.0009 0.0041 0.0004 0.0008 0.0053 0.0005 0.0007 0.0003 0.0153 0.0043 0.0053 0.0021 0.0019 0.003 0.0007 0.0011
Nucleolus 0.0004 0 0 0.0002 0 0 0.0007 0 0.0001 0 0.0003 0.0002 0.0006 0 0 0 0 0
Nucleus 0.0021 0.001 0.0012 0.0034 0.001 0.001 0.003 0.0009 0.0015 0.001 0.0046 0.0019 0.0024 0.0015 0.0009 0.0016 0.0008 0.0009
Peroxisomes 0.0004 0.0001 0.0001 0.002 0.0003 0 0.0039 0 0.0008 0 0.0216 0.0003 0.0001 0 0 0.0001 0.0001 0
Punctate Nuclear 0.0013 0.0001 0.0002 0.0008 0.0001 0.0001 0.0027 0.0001 0.0007 0.0001 0.0026 0.0019 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Vacuole 0.0119 0.0024 0.0081 0.0263 0.0052 0.0042 0.0092 0.0025 0.004 0.0074 0.0109 0.0032 0.0084 0.0043 0.0065 0.0296 0.004 0.004
Vacuole Periphery 0.0034 0.0004 0.0018 0.0063 0.0012 0.0011 0.0015 0.0004 0.0006 0.0013 0.0026 0.0011 0.0015 0.0009 0.0016 0.0045 0.0025 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.5482 33.5918 48.3424 46.9569 50.9351 39.8326 58.4088 52.4469 50.765 52.7902
Translational Efficiency 1.2844 1.6166 0.9341 1.0534 0.9884 1.4736 1.0741 1.0985 1.0349 1.1118

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Snf4

Snf4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snf4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available