Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0.06 0.05 0.06 0.08 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.99 0.93 0.88 – 0.84 0.79 0.81 0.78 0.99 0.98 0.99 1.0 0.99 0.99 0.94 0.94 0.93 0.83 0.87 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.11 – 0.1 0.15 0.13 0.14 0 0 0 0.05 0.07 0.1 0 0 0 0.08 0.07 0.05
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 2 1 3 4 0 0 0 0 0 0 0 0 0 0 2 1
Bud 1 0 1 0 0 4 1 – 0 0 1 1 0 1 0 0 0 0 0 0 1 2 4 6
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 0 2 8 12 – 17 16 16 24 1 3 3 3 2 1 0 0 0 0 0 0
Cytoplasm 187 169 207 171 269 204 323 – 233 236 205 248 145 246 300 60 88 132 153 167 181 138 337 370
Endoplasmic Reticulum 0 0 0 2 3 7 39 – 29 45 33 45 1 4 4 3 6 13 2 1 5 12 28 21
Endosome 0 1 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 1 – 3 5 7 10 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 1 1 0 2 0 0 0 0 1 3 0 1 0 0 0 1
Nucleus 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 0 2 0 0 1 2 – 1 7 2 3 0 0 0 0 0 0 0 0 0 2 4 5
Unique Cell Count 188 170 210 173 272 220 369 278 300 253 319 146 250 304 60 89 133 163 178 195 168 389 416
Labelled Cell Count 189 170 211 173 274 225 378 288 311 267 337 147 254 307 66 97 149 163 178 195 168 389 416


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 285.1 307.5 221.0 209.1 172.8 97.7 106.9 – 78.5 81.3 75.8 77.2 268.3 261.1 238.9 299.7 256.7 240.5 236.0 263.8 267.0
Std Deviation (1e-4) 55.1 65.6 56.5 51.7 42.1 37.0 29.1 – 23.4 25.6 21.8 22.9 79.4 83.4 81.5 102.5 106.0 102.0 74.7 82.4 83.4
Intensity Change (Log2) – – – -0.08 -0.36 -1.18 -1.05 – -1.49 -1.44 -1.54 -1.52 0.28 0.24 0.11 0.44 0.22 0.12 0.09 0.26 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 3.3 3.0 3.3 3.7 0 0 0 0 0 0
Cytoplasm 0.2 0.3 -2.9 -4.6 – -5.4 -6.5 -6.0 -6.8 0.7 -0.1 0.1 0.9 0.2 0.6
Endoplasmic Reticulum 0 0 0 4.9 – 4.8 5.9 5.4 5.7 0 0 0 0 0 4.6
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 220.3663 292.603 245.0069 218.1896 199.3393 225.1041 217.5313 311.7758 263.1561 222.744 291.3043 283.3091 272.0525 322.8615 303.033 294.7286 251.9951 295.797
Actin 0.0053 0.0008 0.0118 0.0018 0.0023 0.0081 0.0123 0.0011 0.0276 0.0036 0.001 0.0032 0.0101 0.001 0.0023 0.002 0.001 0.0048
Bud 0.0041 0.0027 0.0105 0.0037 0.0078 0.0022 0.0055 0.0024 0.0042 0.0132 0.0037 0.0032 0.0053 0.0032 0.0059 0.007 0.0129 0.0043
Bud Neck 0.0015 0.0012 0.002 0.0019 0.0035 0.0041 0.0011 0.0007 0.0019 0.0046 0.0008 0.0024 0.0014 0.0013 0.0013 0.0015 0.0009 0.0022
Bud Periphery 0.0058 0.0019 0.006 0.0034 0.0082 0.0015 0.0087 0.0011 0.0024 0.0061 0.004 0.003 0.0057 0.0012 0.003 0.003 0.0029 0.0024
Bud Site 0.0024 0.003 0.0052 0.0024 0.0027 0.0006 0.0035 0.0018 0.0032 0.0018 0.0017 0.0004 0.0013 0.0021 0.0022 0.0026 0.0019 0.0005
Cell Periphery 0.1047 0.0643 0.0766 0.1401 0.0689 0.0178 0.066 0.0336 0.0419 0.0393 0.1159 0.0085 0.0611 0.0197 0.0401 0.022 0.0169 0.0135
Cytoplasm 0.4 0.7739 0.5727 0.4231 0.4444 0.6339 0.4539 0.8316 0.6736 0.5438 0.2305 0.6673 0.5637 0.8514 0.7289 0.6749 0.3619 0.5788
Cytoplasmic Foci 0.0036 0.0008 0.0053 0.0096 0.0209 0.1249 0.0144 0.001 0.0193 0.0462 0.0033 0.0025 0.0013 0.0008 0.0017 0.0029 0.007 0.0025
Eisosomes 0.0065 0.0001 0.0006 0.0017 0.0008 0.0011 0.0053 0.0001 0.0018 0.0005 0.0007 0.0001 0.0007 0 0.0001 0.0013 0 0.0003
Endoplasmic Reticulum 0.0334 0.0047 0.0055 0.0076 0.0023 0.0293 0.0388 0.0045 0.0078 0.0045 0.0014 0.055 0.0216 0.0083 0.0023 0.002 0.0003 0.0116
Endosome 0.0081 0.001 0.0037 0.0032 0.0052 0.0074 0.0126 0.0012 0.0115 0.0081 0.0045 0.0136 0.0032 0.001 0.0026 0.0022 0.0037 0.005
Golgi 0.0012 0.0002 0.0015 0.0007 0.001 0.0054 0.0033 0.0002 0.0053 0.0017 0.0006 0.0039 0.0008 0.0002 0.0005 0.0004 0.0003 0.0021
Lipid Particles 0.0043 0.0016 0.0037 0.0169 0.0068 0.0309 0.0106 0.0011 0.005 0.008 0.0081 0.0005 0.0017 0.0005 0.0011 0.0025 0.002 0.0021
Mitochondria 0.0176 0.0115 0.0134 0.0312 0.0204 0.0125 0.0404 0.0038 0.0141 0.0101 0.0097 0.0312 0.0143 0.0033 0.0145 0.0163 0.0113 0.0384
None 0.0076 0.0013 0.003 0.0076 0.0211 0.029 0.014 0.0008 0.0074 0.0139 0.0018 0.0034 0.0038 0.0009 0.0019 0.0049 0.0034 0.0043
Nuclear Periphery 0.0716 0.0102 0.0217 0.0268 0.0211 0.0441 0.0671 0.0063 0.0123 0.0151 0.0115 0.0506 0.0683 0.0119 0.0165 0.0259 0.0143 0.085
Nucleolus 0.0164 0.0046 0.0186 0.0123 0.0383 0.0061 0.0184 0.0035 0.015 0.005 0.0171 0.001 0.0033 0.0019 0.0077 0.024 0.0414 0.0217
Nucleus 0.0328 0.0102 0.0201 0.0152 0.0432 0.0065 0.0348 0.0068 0.0135 0.0183 0.0193 0.0191 0.038 0.0162 0.024 0.0527 0.1198 0.0574
Peroxisomes 0.0006 0.0002 0.0008 0.0016 0.0013 0.0027 0.0021 0.0002 0.0012 0.0018 0.0004 0.0003 0.0003 0.0002 0.0004 0.0006 0.0009 0.0004
Punctate Nuclear 0.0003 0.0001 0.0002 0.0005 0.0005 0.0026 0.0012 0.0001 0.0006 0.0007 0.0001 0.0002 0.0003 0.0001 0.0002 0.0005 0.0003 0.001
Vacuole 0.2155 0.0917 0.176 0.2188 0.2003 0.02 0.1384 0.0847 0.1041 0.2104 0.4272 0.0498 0.1361 0.0639 0.1032 0.0681 0.223 0.0567
Vacuole Periphery 0.0567 0.0141 0.0412 0.07 0.0791 0.0094 0.0479 0.0134 0.0263 0.0433 0.1367 0.0808 0.0578 0.0109 0.0398 0.0827 0.1739 0.1048

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3567.5057 3758.4854 4661.3208 3506.4992 3946.2144 3959.2651 3353.0061 2967.8289 3463.1629 2901.999
Translational Efficiency 0.7011 0.738 0.6743 0.8772 0.8209 0.6644 0.7797 0.9624 0.7719 0.9095

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpl1b

Rpl1b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl1b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available