Standard name
Human Ortholog
Description Essential beta'-coat protein of the COPI coatomer; involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.21 0.12 0.15 0.07 0.05 0.06 0.05 – 0.05 0.06 0.1 0.08 0.09 0 0.09 0.13 0.06 0 0 0.06 0.05 0 0.07
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.09 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.11 0 0 0 0 0 0 – 0 0 0 0.07 0.07 0.06 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Golgi 0.84 0.61 0.78 0.69 0.63 0.2 0.24 0.26 – 0.18 0.08 0.2 0.89 0.89 0.85 0.45 0.68 0.78 0.89 0.76 0.88 0.86 0.88 0.87
Mitochondria 0.35 0.55 0.66 0.73 0.67 0.89 0.81 0.77 – 0.87 0.91 0.81 0.1 0.1 0.05 0.15 0.1 0.07 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.07 0 0.07 0.07 0.12 – 0.07 0.09 0.07 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.21 0.1 0.05 0 0.08 0 0.07 0.05 – 0.05 0 0 0.15 0.15 0.22 0.2 0.16 0.12 0 0.11 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 47 61 56 32 24 43 39 – 35 28 77 24 27 13 17 50 14 0 8 33 11 16 35
Bud 0 2 5 4 1 3 4 9 – 11 16 8 1 1 0 1 1 3 0 1 1 0 3 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 2 1 – 3 3 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 1 3 3 3 1 – 0 3 6 1 1 0 5 0 0 0 0 0 0 0 0
Cytoplasm 0 13 12 13 11 10 14 15 – 16 4 19 11 11 14 17 34 20 0 4 0 0 0 0
Endoplasmic Reticulum 3 24 12 11 6 10 14 6 – 12 6 28 20 19 17 7 18 7 0 5 0 1 2 4
Endosome 0 0 1 0 0 1 0 2 – 0 0 6 1 1 1 14 11 3 0 4 5 3 2 4
Golgi 36 135 401 263 303 104 187 218 – 135 38 154 264 260 255 81 257 173 57 207 467 203 353 461
Mitochondria 15 122 337 279 322 475 626 649 – 643 447 607 30 30 15 27 39 16 2 7 8 11 11 9
Nucleus 0 0 0 0 1 2 4 1 – 1 2 0 0 0 0 2 2 1 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 2 0 1 – 1 2 2 0 0 0 4 1 0 0 0 0 0 0 0
Nucleolus 1 3 10 25 17 38 52 101 – 53 42 55 3 3 2 14 19 11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 1 1 0 0 4 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 7 4 0 0 0 1 0 0 1
Vac/Vac Membrane 9 21 28 15 37 14 51 46 – 34 15 22 45 45 65 37 59 27 1 29 4 4 8 4
Unique Cell Count 43 220 514 383 480 533 769 842 743 493 752 295 291 299 181 378 223 64 272 533 238 401 529
Labelled Cell Count 66 368 870 667 733 687 1000 1089 945 606 987 400 398 382 233 496 276 64 272 533 238 401 529


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 36.4 34.2 30.0 28.1 27.4 23.8 23.4 22.7 – 21.7 19.6 20.7 34.2 34.2 37.1 15.1 22.0 28.2 35.0 36.2 37.9
Std Deviation (1e-4) 7.1 7.5 6.6 6.7 5.1 5.2 5.1 5.2 – 5.5 4.7 5.0 8.3 8.3 10.1 5.6 9.3 10.5 6.3 6.7 7.6
Intensity Change (Log2) – – – -0.09 -0.13 -0.33 -0.36 -0.4 – -0.47 -0.61 -0.54 0.19 0.19 0.31 -0.99 -0.45 -0.09 0.22 0.27 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.2 -2.8 -4.4 -4.0 -4.9 – -4.7 -3.5 -0.9 -1.7 -1.1 -3.6 -0.9 0.6 -2.3
Bud 0 0 0 0 0.2 – 0.8 2.5 0.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0 -0.5 -0.6 -0.7 – -0.2 -1.9 0.2 1.1 1.2 1.8 4.1 4.4 4.1
Endoplasmic Reticulum 0.5 -1.3 -0.5 -0.6 -2.5 – -0.9 -1.3 1.4 3.1 3.0 2.5 0 2.0 0.6
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 3.5 0
Golgi -3.2 -5.2 -18.9 -18.9 -18.7 – -21.1 -22.5 -20.3 4.1 4.0 2.5 -8.3 -3.4 -0.1
Mitochondria 2.3 0.5 9.1 6.4 4.6 – 8.8 9.6 6.1 -15.2 -15.1 -16.8 -11.7 -16.5 -14.6
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 3.5 1.5 4.0 3.9 6.5 – 4.1 4.7 4.3 0 0 0 3.7 2.6 2.2
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.1 1.4 -2.3 0.9 0 – -0.7 -1.9 -2.3 4.7 4.8 7.0 6.0 5.1 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 26.8834 33.475 31.5703 28.1366 27.2891 31.3948 23.986 34.0803 32.3313 29.7964 30.2942 31.8712 26.4887 34.8918 31.907 29.6598 26.2146 33.2399
Actin 0.0287 0.0112 0.0077 0.0079 0.0063 0.0019 0.0403 0.0141 0.0267 0.0484 0.0177 0.0101 0.0397 0.0041 0.0241 0.0048 0.0058 0.0018
Bud 0.0003 0.0008 0.0002 0.0009 0.0002 0 0.0006 0.0001 0.0003 0.0004 0.0003 0.0001 0.0006 0.0001 0.0004 0.0001 0.0002 0
Bud Neck 0.0003 0.0003 0.0001 0.0004 0.0001 0.0001 0.0005 0.0001 0.0002 0.0006 0.0001 0.0001 0.0022 0.0001 0.0004 0.0001 0.0002 0
Bud Periphery 0.0003 0.0005 0.0002 0.0015 0.0003 0 0.0008 0.0001 0.0004 0.0005 0.0003 0.0001 0.0009 0.0001 0.0004 0.0002 0.0002 0
Bud Site 0.0012 0.0026 0.0002 0.0039 0.0002 0 0.002 0.0003 0.001 0.0012 0.0013 0.0002 0.0015 0.0001 0.0022 0.0001 0.0011 0
Cell Periphery 0.0003 0.0011 0.0002 0.0009 0.0003 0.0001 0.0005 0.0001 0.0003 0.0003 0.0003 0.0001 0.0004 0.0001 0.0003 0.0004 0.0002 0.0001
Cytoplasm 0.0023 0.0038 0.002 0.012 0.0014 0.0002 0.0054 0.0003 0.0005 0.0008 0.0014 0.0002 0.0012 0.0009 0.0025 0.0007 0.0105 0.0001
Cytoplasmic Foci 0.0259 0.0134 0.0088 0.0081 0.0266 0.0045 0.0191 0.0067 0.0126 0.0084 0.02 0.0033 0.0161 0.009 0.0085 0.0111 0.0171 0.0041
Eisosomes 0.0003 0.0012 0.0001 0.0001 0.0001 0 0.0006 0.0001 0.0005 0.0002 0.0002 0.0001 0.0005 0.0001 0.0003 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0014 0.0015 0.0008 0.0046 0.0007 0.0005 0.0044 0.0003 0.0007 0.0016 0.0009 0.0008 0.0011 0.0004 0.0014 0.001 0.0036 0.0003
Endosome 0.0876 0.1243 0.0976 0.094 0.0838 0.0642 0.0665 0.0537 0.0713 0.1787 0.0505 0.0447 0.0826 0.0988 0.0737 0.0689 0.156 0.0405
Golgi 0.7135 0.7528 0.7963 0.6498 0.7581 0.8829 0.5888 0.8461 0.74 0.6404 0.7862 0.8626 0.6783 0.8061 0.7518 0.7059 0.7289 0.8636
Lipid Particles 0.015 0.0123 0.0086 0.0106 0.0139 0.0027 0.0321 0.0035 0.0253 0.014 0.0198 0.0059 0.0199 0.0031 0.0055 0.0246 0.0226 0.0083
Mitochondria 0.0657 0.0272 0.0302 0.0715 0.0627 0.0294 0.1829 0.0637 0.0895 0.0362 0.0253 0.051 0.1213 0.034 0.0883 0.0688 0.0088 0.0626
None 0.0025 0.0001 0.0006 0.0027 0.0015 0 0.0049 0 0.0002 0.0002 0.0016 0.0001 0.0025 0 0.0066 0.0004 0.0046 0.0001
Nuclear Periphery 0.005 0.0016 0.0019 0.0035 0.0007 0.0026 0.0091 0.0001 0.0005 0.0025 0.001 0.001 0.0014 0.0002 0.0011 0.0014 0.0024 0.0003
Nucleolus 0.0035 0.0002 0.0003 0.001 0.0007 0 0.0013 0 0.0002 0.0001 0.0002 0 0.0006 0 0.0001 0.0002 0.0003 0.0013
Nucleus 0.0057 0.0004 0.0004 0.0046 0.0005 0 0.0076 0 0.0001 0.0002 0.0002 0.0001 0.0005 0.0001 0.0002 0.0002 0.0007 0.0001
Peroxisomes 0.0072 0.0004 0.0004 0.0294 0.0021 0 0.0031 0.0016 0.0022 0.0011 0.0115 0.0004 0.0042 0.0001 0.002 0.0021 0.0019 0.0006
Punctate Nuclear 0.0037 0.0001 0.0004 0.0006 0.0004 0 0.0056 0 0.0002 0.0001 0.0017 0.0001 0.0023 0 0.0002 0.0001 0.0031 0.0001
Vacuole 0.0062 0.0197 0.0117 0.0399 0.0056 0.0012 0.0046 0.0008 0.0049 0.0149 0.0059 0.0033 0.004 0.0138 0.0055 0.0143 0.0083 0.0014
Vacuole Periphery 0.0234 0.0246 0.0311 0.0521 0.0339 0.0096 0.0192 0.0082 0.0223 0.0495 0.0536 0.0157 0.0183 0.0288 0.0246 0.0944 0.0235 0.0146

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 173.3626 167.3515 150.2246 174.8161 176.8172 138.3655 169.2089 167.9034 175.4201 191.5429
Translational Efficiency 1.1205 1.1298 1.1008 1.1124 1.0082 1.1914 1.1108 0.8549 0.9033 0.9354

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential beta'-coat protein of the COPI coatomer; involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
Localization
Cell Percentages nucleus (15%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec27

Sec27


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec27-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available