Standard name
Human Ortholog
Description Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.09 0.34 0.16 0.17 0.25 0.21 0.16 0.15 0.16 0.15 0.17 0.13 0.2 0.2 0.22 0.22 0.23 0.09 0.05 0.17 0.07 0.06 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.18 0.13 0.46 0.42 0.53 0.32 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.53 0.54 0.53 0.67 0.59 0.52 0.57 0.59 0.51 0.44 0.46 0.42 0.57 0.54 0.5 0.43 0.5 0.49 0.61 0.61 0.4 0.41 0.46 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.33 0.49 0.13 0.14 0.27 0.23 0.18 0.24 0.17 0.22 0.17 0.25 0.32 0.3 0.36 0.39 0.31 0.3 0.15 0.19 0.26 0.29 0.26 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 4 1
Bud 1 0 0 1 1 0 2 1 3 6 7 5 0 0 0 0 0 0 4 11 5 13 19 19
Bud Neck 1 0 1 2 0 1 2 1 0 1 1 2 0 0 0 0 0 1 1 2 1 2 4 7
Bud Site 0 0 0 0 0 0 2 2 5 4 7 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0
Cytoplasm 40 39 77 41 59 67 68 56 58 74 59 63 41 57 66 105 88 70 21 23 57 11 23 28
Endoplasmic Reticulum 1 0 4 0 2 1 1 0 1 1 1 0 1 1 0 29 23 13 1 0 2 1 3 6
Endosome 1 8 2 2 6 2 7 4 1 3 2 9 1 4 9 19 14 13 7 12 5 1 7 8
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 2 0 1 0 2
Mitochondria 3 13 6 10 12 38 59 47 176 196 203 118 18 2 2 9 7 9 2 5 13 4 14 7
Nucleus 107 222 121 167 206 138 186 210 193 208 177 158 182 159 166 204 198 150 146 290 136 72 187 141
Nuclear Periphery 1 0 0 0 0 0 1 0 0 3 1 1 1 0 0 1 0 0 3 3 1 1 3 4
Nucleolus 0 1 0 0 1 0 0 0 0 1 0 0 0 1 2 1 0 0 3 13 2 1 2 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 1 0 2
SpindlePole 0 3 0 2 2 0 1 5 0 1 1 2 0 1 0 2 3 0 6 14 13 4 15 14
Vac/Vac Membrane 68 203 29 35 95 60 58 86 63 104 65 94 101 87 120 186 124 92 35 90 87 50 105 88
Unique Cell Count 203 411 227 250 347 266 328 356 381 470 382 372 317 292 332 479 398 306 241 476 341 175 404 347
Labelled Cell Count 223 490 241 261 384 307 387 412 500 602 524 462 345 312 368 560 458 349 241 476 341 175 404 347


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.4 5.5 5.5 6.1 5.0 5.1 5.3 4.5 4.5 4.2 5.0 5.7 5.9 5.7 7.4 7.6 7.5 5.0 5.0 5.6
Std Deviation (1e-4) 0.8 0.6 1.1 1.2 1.1 1.3 1.3 0.8 1.1 1.1 1.0 1.4 1.5 1.5 1.4 1.5 1.7 1.7 1.3 1.7 2.0
Intensity Change (Log2) – – – -0.01 0.14 -0.13 -0.1 -0.07 -0.29 -0.28 -0.4 -0.15 0.05 0.1 0.06 0.43 0.47 0.44 -0.15 -0.12 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -4.4 -4.7 -2.1 -3.5 -5.1 -5.4 -5.5 -5.3 -4.8 -5.9 -3.7 -3.7 -3.4 -3.2 -2.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.5 2.4 1.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 2.3 2.0 2.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 0.5 4.5 5.5 4.3 0 0 0 8.5 1.7 0 0 0 0 0.2
Nucleus 3.0 1.4 -0.3 0.8 1.4 -0.6 -2.2 -1.7 -2.6 1.0 0.3 -0.8 -2.7 -0.9 -1.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.4 4.2 2.8 1.6 3.4 1.3 2.9 1.4 3.7 5.1 4.6 6.1 7.0 5.1 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8786 3.7778 3.2323 2.5679 2.77 2.973 2.2818 2.337 1.9427 2.0103 1.8765 1.8414 1.5355 1.9365 1.7369 1.4674 1.9115 1.7668
Actin 0.036 0.0113 0.0076 0.0245 0.0072 0.0047 0.0193 0.0002 0.0023 0.0185 0.0107 0.0033 0.003 0.0001 0.0023 0 0.0002 0
Bud 0.0005 0.0003 0.0002 0.0001 0.0004 0.0002 0.0005 0.0002 0.0047 0.0029 0.0042 0.0004 0.0001 0.0003 0.0001 0 0 0
Bud Neck 0.0068 0.0004 0.0072 0.0003 0.001 0.0054 0.0027 0.0008 0.0009 0.0029 0.001 0.0041 0.0009 0.0003 0.0004 0.0003 0.0009 0.0039
Bud Periphery 0.0009 0.0002 0.0004 0.0001 0.0026 0.0002 0.0009 0.0004 0.0011 0.0048 0.0042 0.0016 0.0001 0.0001 0.0001 0 0 0
Bud Site 0.0034 0.0043 0.0036 0.0001 0.0005 0.0009 0.0067 0.0034 0.0013 0.0102 0.0101 0.0005 0.0003 0.0003 0.0004 0 0.0001 0
Cell Periphery 0.0007 0 0.0004 0 0.0003 0 0.0004 0.0004 0.0001 0.0005 0.0002 0.0002 0 0 0 0 0 0
Cytoplasm 0.0148 0.0093 0.0214 0.0399 0.0052 0.0054 0.0063 0.0066 0.0037 0.0049 0.014 0.0037 0.0063 0.0111 0.0033 0.0007 0.0014 0.001
Cytoplasmic Foci 0.0198 0.0098 0.0048 0.0076 0.0029 0.0054 0.0461 0.0003 0.005 0.007 0.0126 0.0012 0.0074 0.0002 0.0048 0 0 0.0001
Eisosomes 0.0008 0.0001 0.0004 0.0002 0.0013 0 0.0001 0 0 0.0003 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.009 0.0003 0.0014 0.002 0.0005 0.0005 0.0055 0.0005 0.0007 0.0029 0.0009 0.001 0.0005 0.0003 0.0011 0 0.0002 0
Endosome 0.0409 0.0019 0.0026 0.0064 0.0011 0.0027 0.0506 0.0018 0.0069 0.022 0.0107 0.0042 0.003 0.0002 0.0055 0 0.0003 0.0001
Golgi 0.012 0.0025 0.0015 0.0166 0.0007 0.0037 0.0195 0.0001 0.0022 0.019 0.0067 0.0025 0.0008 0 0.0011 0 0 0
Lipid Particles 0.0438 0.003 0.0033 0.0018 0.0011 0.0043 0.0296 0.0008 0.0058 0.0139 0.0016 0.0036 0.0022 0 0.0021 0 0 0
Mitochondria 0.0128 0.002 0.0169 0.0006 0.0504 0.0041 0.0047 0.0014 0.0077 0.0264 0.0053 0.0103 0.0002 0.0002 0.0003 0.0001 0.0003 0.0001
None 0.0237 0.0284 0.0309 0.1049 0.1932 0.0278 0.0025 0.0012 0.001 0.0117 0.0008 0.0009 0.0116 0.0068 0.0017 0.0002 0.0005 0.0001
Nuclear Periphery 0.0175 0.0091 0.011 0.0082 0.0079 0.0054 0.0447 0.0268 0.0192 0.0164 0.0155 0.0128 0.0111 0.0143 0.0144 0.0021 0.0323 0.002
Nucleolus 0.0099 0.0082 0.006 0.0051 0.007 0.0164 0.0068 0.0063 0.0033 0.0029 0.0168 0.0125 0.0069 0.0065 0.006 0.0047 0.0109 0.0118
Nucleus 0.6882 0.8861 0.8542 0.7756 0.708 0.8963 0.6966 0.9425 0.904 0.7741 0.8556 0.9139 0.7783 0.8885 0.8135 0.9214 0.8435 0.876
Peroxisomes 0.0098 0.0082 0.0116 0.0009 0.0007 0.0074 0.0239 0.0001 0.0158 0.0154 0.0157 0.0006 0.0036 0 0.0011 0 0 0
Punctate Nuclear 0.0408 0.0143 0.0134 0.0046 0.0059 0.0085 0.0241 0.0048 0.0106 0.0357 0.0063 0.0208 0.1631 0.0696 0.1412 0.0703 0.1089 0.1046
Vacuole 0.0058 0.0002 0.0011 0.0002 0.0009 0.0006 0.0063 0.0008 0.0029 0.0036 0.0056 0.0014 0.0003 0.0012 0.0003 0 0.0003 0.0001
Vacuole Periphery 0.0022 0.0001 0.0002 0.0001 0.001 0.0002 0.0021 0.0005 0.0008 0.0041 0.0017 0.0006 0.0002 0.0002 0.0002 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.6709 7.5792 10.1769 15.4881 10.2074 11.7018 14.5968 18.2259 17.1633 13.3509
Translational Efficiency 0.9807 0.904 0.9191 0.7684 0.6946 0.8118 0.6399 0.5639 0.6557 0.6536

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock
Localization
Cell Percentages nucleus (70%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hul5

Hul5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hul5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available