Standard name
Human Ortholog
Description Zn(II)2Cys6 family transcription factor; positively regulates sterol uptake genes under anaerobic conditions; involved in hypoxic gene expression; represses filamentation-inducing genes during vegetative growth; positively regulates mating with SUT2 by repressing expression of genes that act as mating inhibitors; repressed by STE12; relocalizes from the nucleus to the cytoplasm upon DNA replication stress; SUT1 has a paralog, SUT2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.06 0 0.64 0.73 0.65 0.54 0.35 0.39 0.2 0.32 0.14 0.41 0.83 0.28 0.23 0 0 0 0 0.09 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.12 0.14 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.39 0 0 0.1 0.2 0.24 0.69 0.55 0.7 0.6 0 0 0 0.11 0.06 0 0 0 0 0 0
Nucleus 0.8 0.72 0.88 0.23 0.13 0.1 0.23 0.2 0.3 0.35 0.28 0.81 0.41 0.07 0.39 0.45 0.68 0.65 0.7 0.61 0.53 0.39
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0.07 0.06 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.07 0.07 0.06 0.05 0.06 0.06 0.06 0.12
Vac/Vac Membrane 0.08 0 0 0.14 0 0 0 0 0 0 0 0.05 0.11 0.14 0.21 0.16 0.09 0.12 0.08 0.13 0.15 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0
Bud 0 0 0 0 0 0 1 1 1 1 2 0 0 0 0 1 4 5 0 4 8 6
Bud Neck 0 0 0 0 0 4 4 0 0 2 3 1 3 1 0 0 0 1 0 1 0 5
Bud Site 0 0 0 0 0 0 2 3 2 3 8 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 17 20 1 14 22 51 63 54 64 24 69 23 99 244 56 30 7 13 0 11 22 29
Endoplasmic Reticulum 0 0 0 0 0 1 2 1 0 1 1 0 0 1 7 11 0 0 0 2 2 1
Endosome 4 1 0 0 0 1 0 1 0 0 1 0 13 4 24 18 4 6 0 2 2 8
Golgi 3 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 1 0 0 0
Mitochondria 22 133 1 0 3 16 28 107 91 83 128 3 2 1 21 8 4 11 0 3 7 9
Nucleus 202 246 29 5 4 8 27 31 50 42 59 132 99 20 77 59 198 255 46 176 135 108
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 6 1 0 0 0 0 0 0 2 0 2 1 0 0 1 1 20 25 3 20 16 20
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 6 10 1 0 1 4 5 0 3 0 4 5 3 1 14 9 17 18 4 16 15 32
Vac/Vac Membrane 19 13 1 3 1 2 3 3 1 5 8 8 26 40 42 21 25 47 5 37 38 44
Unique Cell Count 251 340 33 22 30 79 117 156 165 119 214 163 239 295 197 131 292 394 67 288 257 279
Labelled Cell Count 279 424 34 22 31 87 136 201 214 161 285 173 245 312 244 159 292 394 67 288 257 279


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.0 5.6 4.9 4.1 3.9 4.3 3.2 3.4 3.3 3.4 5.4 5.1 4.6 4.5 5.5 5.6 5.8 5.8 5.6
Std Deviation (1e-4) 0.8 0.8 1.0 1.3 0.8 1.2 2.0 1.1 0.9 0.8 0.6 0.8 1.1 0.8 1.5 1.6 1.3 1.4 1.6 1.2
Intensity Change (Log2) -0.19 -0.47 -0.51 -0.37 -0.79 -0.73 -0.77 -0.73 -0.05 -0.15 -0.29 -0.33 -0.03 -0.01 0.04 0.04 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8795 3.5722 3.556 2.2762 2.5653 2.8033 3.2936 3.3701 3.3015 2.9482 3.1685 3.2855 1.1009 1.25 1.0491 0.853 0.6036 0.929
Actin 0.0203 0 0.0229 0.0005 0.0121 0.0019 0.0076 0.0005 0.0064 0.0011 0.0001 0.0001 0.0322 0.0001 0.0016 0.0001 0.0001 0.0012
Bud 0.0003 0 0.0004 0.0002 0.0004 0.0001 0.0002 0.0001 0.0002 0.0024 0 0 0.0013 0.0001 0.0002 0 0 0.0001
Bud Neck 0.0009 0.0002 0.0009 0.001 0.0016 0.0034 0.0015 0.001 0.0004 0.001 0.0005 0.0021 0.0032 0.0003 0.0024 0.0052 0.0014 0.0031
Bud Periphery 0.0007 0 0.0012 0.0002 0.0009 0.0002 0.0002 0.0001 0.0002 0.0052 0 0 0.0028 0.0001 0.0002 0 0.0001 0.0002
Bud Site 0.0011 0.0006 0.0029 0.0011 0.0016 0.0002 0.0007 0.0016 0.0006 0.0212 0 0.0001 0.0046 0.0004 0.003 0.0001 0.0001 0.0002
Cell Periphery 0.0002 0 0.0005 0 0.0001 0 0.0001 0 0.0001 0.0026 0 0 0.0007 0 0.0001 0 0 0
Cytoplasm 0.0205 0.0079 0.017 0.0038 0.0079 0.0064 0.0251 0.0193 0.0088 0.0099 0.008 0.0151 0.0293 0.0176 0.0174 0.0119 0.0031 0.0096
Cytoplasmic Foci 0.0032 0.0001 0.008 0.0004 0.0336 0.001 0.0051 0.0045 0.0003 0.0012 0.0002 0.0005 0.0114 0.0005 0.0027 0.0009 0.0001 0.0008
Eisosomes 0.0002 0 0.0002 0 0.0001 0.0001 0.0002 0 0.0002 0.0001 0 0 0.0014 0 0.0001 0 0 0
Endoplasmic Reticulum 0.003 0.0001 0.0021 0.0004 0.0009 0.0004 0.0018 0.0008 0.0006 0.0008 0.0001 0.0004 0.0048 0.0007 0.0005 0.0014 0.0002 0.0009
Endosome 0.0121 0.0001 0.0066 0.0003 0.0241 0.0027 0.0153 0.0009 0.0016 0.0028 0.0001 0.0004 0.0193 0.0007 0.0015 0.0026 0.0003 0.0013
Golgi 0.0023 0 0.0044 0.0001 0.0192 0.0013 0.0048 0.0001 0.0008 0.0012 0 0 0.006 0 0.0008 0 0 0.0002
Lipid Particles 0.0074 0 0.0092 0 0.0712 0.0046 0.0097 0.0002 0.0001 0.0002 0 0 0.0116 0 0.0024 0.0001 0 0.0004
Mitochondria 0.0015 0.0002 0.0062 0.0005 0.0259 0.0005 0.0019 0.0003 0.0023 0.0047 0.0001 0.0002 0.0105 0.0003 0.0006 0.0002 0.0003 0.0006
None 0.0919 0.0307 0.0668 0.0009 0.0249 0.0266 0.1337 0.0145 0.0787 0.0029 0.148 0.0434 0.0889 0.0707 0.0703 0.0015 0.0015 0.0364
Nuclear Periphery 0.0113 0.0023 0.0161 0.0083 0.0266 0.0039 0.0061 0.0136 0.0114 0.0046 0.0063 0.0051 0.0186 0.0062 0.0049 0.0179 0.0094 0.0108
Nucleolus 0.022 0.0146 0.0195 0.0102 0.0787 0.0289 0.0181 0.0149 0.0148 0.0167 0.0174 0.0406 0.0172 0.0145 0.017 0.0167 0.0508 0.027
Nucleus 0.7598 0.9377 0.7728 0.9223 0.5256 0.8985 0.7224 0.7915 0.8546 0.9018 0.8007 0.8504 0.66 0.8664 0.8401 0.9067 0.8535 0.8683
Peroxisomes 0.0016 0 0.012 0 0.0728 0.0009 0.0009 0.0002 0.0001 0.0001 0 0 0.0049 0 0.0053 0 0 0.0001
Punctate Nuclear 0.0305 0.0051 0.0222 0.0496 0.0697 0.0181 0.0421 0.1354 0.0168 0.015 0.0182 0.0412 0.0591 0.0195 0.0284 0.0336 0.0787 0.038
Vacuole 0.0077 0.0003 0.0061 0.0002 0.0011 0.0004 0.002 0.0003 0.0005 0.0041 0.0001 0.0003 0.0103 0.0015 0.0005 0.0007 0.0002 0.0006
Vacuole Periphery 0.0013 0 0.0019 0.0001 0.0009 0.0002 0.0004 0.0001 0.0004 0.0005 0 0.0001 0.0018 0.0002 0.0001 0.0003 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.034 14.2477 23.5845 14.0436 7.1531 11.0279 17.1761 11.5927 10.541 12.1888
Translational Efficiency 0.6905 0.82 0.4717 0.7896 1.289 1.147 0.5531 0.8961 0.9143 0.5968

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Zn(II)2Cys6 family transcription factor; positively regulates sterol uptake genes under anaerobic conditions; involved in hypoxic gene expression; represses filamentation-inducing genes during vegetative growth; positively regulates mating with SUT2 by repressing expression of genes that act as mating inhibitors; repressed by STE12; relocalizes from the nucleus to the cytoplasm upon DNA replication stress; SUT1 has a paralog, SUT2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Sut1

Sut1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sut1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available