Standard name
Human Ortholog
Description DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0.05 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.74 0.92 0.86 0.74 0.77 0.79 0.86 0.75 – 0.64 0.75 0.66 0.71 0.71 0.39 0.34 0.34 0.56 0.48 0.53 0.7 0.75 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0.08 0.08 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.13 0 0 0 0.21 0.15 0.13 0.29 – 0.56 0.27 0.05 0 0 0.06 0 0.09 0 0.08 0.1 0.12 0.09 0.09
Nucleus 0.09 0.11 0 0.06 0.13 0.08 0.07 0 0.05 – 0.05 0.06 0.25 0.19 0.16 0.26 0.29 0.28 0.14 0.16 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.07 0.08 0 0 0
Vac/Vac Membrane 0.09 0.11 0 0 0.07 0 0 0 0 – 0 0 0.06 0.07 0.09 0.3 0.27 0.31 0.1 0.12 0.11 0.05 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 2 4 5 8
Bud 0 3 0 0 1 0 1 0 2 – 5 4 0 0 0 0 1 1 2 3 1 6 5 12
Bud Neck 0 1 8 9 11 5 14 13 2 – 6 4 1 0 0 0 1 1 1 1 0 1 2 4
Bud Site 0 0 0 0 0 0 2 0 3 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 1 1 1 2 1 – 0 1 0 0 0 1 0 1 0 0 2 2 5 2
Cytoplasm 73 104 166 181 163 142 238 209 159 – 109 121 150 179 212 111 62 55 64 77 107 140 261 202
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 – 0 0 0 0 1 22 12 12 1 0 2 1 2 5
Endosome 1 0 0 0 1 0 1 0 1 – 0 0 1 13 8 22 14 9 1 2 2 0 2 3
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 3 0 1 0 1 0 0 1
Mitochondria 8 18 0 7 8 38 46 32 61 – 95 43 11 3 6 16 5 14 3 12 20 24 32 28
Nucleus 8 16 2 12 29 14 21 6 11 – 8 9 57 47 49 76 53 45 16 26 10 0 0 1
Nuclear Periphery 0 1 0 0 0 1 0 0 0 – 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 1 – 0 0 1 2 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 1 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 6 3 5 3 6 5 4 – 1 4 2 2 2 2 3 1 5 11 15 1 5 2
Vac/Vac Membrane 8 15 3 3 15 0 3 0 4 – 0 0 14 17 28 86 49 49 11 19 21 10 15 18
Unique Cell Count 93 140 181 211 220 184 300 244 212 171 161 226 253 298 287 180 160 115 163 203 202 349 302
Labelled Cell Count 98 158 187 217 235 204 333 267 249 224 187 238 264 309 337 203 189 115 163 203 202 349 302


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.8 5.3 3.9 5.3 3.9 3.8 4.0 4.2 – 2.8 3.3 4.1 4.4 4.3 6.3 6.6 6.4 4.0 3.9 4.5
Std Deviation (1e-4) 1.0 0.7 3.6 1.1 3.7 2.6 1.3 1.5 4.3 – 0.9 1.3 1.4 1.8 1.7 1.7 2.4 2.8 1.8 1.7 2.5
Intensity Change (Log2) – – – -0.43 0.01 -0.43 -0.49 -0.42 -0.32 – -0.91 -0.67 -0.36 -0.26 -0.3 0.25 0.33 0.27 -0.4 -0.43 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.6 6.9 7.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9376 1.7016 1.4381 1.4548 0.5079 1.1765 -0.7885 -0.9048 -0.703 -1.0221 -1.3042 -1.1778 -1.3299 -0.6316 -0.4632 -0.8642 -0.5936 -0.6729
Actin 0.0277 0.0002 0.0169 0.0031 0.0009 0.0142 0.0309 0.0007 0.0084 0.0003 0.0002 0.0051 0.0089 0.0002 0.0051 0.0007 0.0007 0.0006
Bud 0.0009 0.0011 0.0001 0.0006 0.0002 0.001 0.002 0.0012 0.0058 0 0.0002 0.0005 0.0048 0.0042 0.0127 0.0139 0.0136 0.0103
Bud Neck 0.0028 0.0003 0.0004 0.0007 0.0027 0.007 0.0042 0.0008 0.0033 0.0008 0.004 0.0042 0.0009 0.0006 0.0007 0.0005 0.0014 0.0038
Bud Periphery 0.0032 0.0006 0.0002 0.0007 0.0004 0.0053 0.0054 0.0013 0.0041 0 0.0001 0.0014 0.0012 0.001 0.0041 0.0056 0.0056 0.0041
Bud Site 0.005 0.005 0.0005 0.0025 0.0005 0.0004 0.0057 0.0193 0.0151 0.0001 0.0001 0.0003 0.0066 0.0062 0.0052 0.0004 0.0013 0.0008
Cell Periphery 0.0011 0.0002 0.0001 0.0001 0.0002 0.0004 0.0006 0.0002 0.0007 0 0 0.0002 0.0003 0.0001 0.0002 0.0001 0.0002 0.0002
Cytoplasm 0.0689 0.0999 0.0849 0.0888 0.1146 0.1268 0.1051 0.1684 0.1017 0.0819 0.1889 0.2272 0.0769 0.0959 0.0697 0.0878 0.1219 0.1302
Cytoplasmic Foci 0.052 0.002 0.0155 0.0211 0.0455 0.0071 0.0363 0.01 0.0174 0.0025 0.0081 0.0137 0.0188 0.006 0.0106 0.0027 0.0273 0.0156
Eisosomes 0.0013 0.0002 0.0003 0.0001 0.0002 0.0004 0.0005 0.0001 0.0003 0 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0089 0.0015 0.0026 0.0019 0.0032 0.0024 0.0103 0.0031 0.0033 0.001 0.0036 0.0028 0.002 0.0011 0.001 0.0019 0.0018 0.0012
Endosome 0.0248 0.0011 0.0123 0.0196 0.011 0.0038 0.0267 0.0056 0.0098 0.0024 0.0046 0.0055 0.0024 0.0011 0.0026 0.0026 0.0035 0.0012
Golgi 0.0097 0 0.0044 0.0083 0.001 0.0039 0.0111 0.0003 0.004 0.0001 0.0001 0.0005 0.0004 0.0001 0.0003 0.0001 0.0002 0.0001
Lipid Particles 0.0448 0.0002 0.0153 0.0182 0.012 0.0079 0.0336 0.0017 0.0092 0.0006 0.0004 0.001 0.0011 0.0004 0.0016 0.0005 0.0008 0.0003
Mitochondria 0.0104 0.0003 0.0106 0.077 0.0037 0.0073 0.0238 0.0009 0.0126 0.0009 0.0006 0.0013 0.0015 0.0005 0.0025 0.0007 0.0023 0.001
None 0.6204 0.7204 0.4449 0.3574 0.6499 0.6664 0.548 0.459 0.2594 0.2023 0.2761 0.417 0.7301 0.5323 0.2594 0.3916 0.5064 0.5025
Nuclear Periphery 0.0061 0.0039 0.0084 0.0054 0.0145 0.0032 0.0164 0.0076 0.0203 0.0137 0.0069 0.0132 0.0047 0.0057 0.0124 0.0133 0.0133 0.0049
Nucleolus 0.0022 0.0025 0.0058 0.0037 0.0093 0.0017 0.0026 0.0033 0.0081 0.0091 0.0069 0.0034 0.0066 0.0081 0.0078 0.0025 0.0098 0.0101
Nucleus 0.0544 0.135 0.327 0.3214 0.0776 0.1136 0.0797 0.2678 0.4588 0.5907 0.4167 0.2541 0.1022 0.2694 0.5126 0.3546 0.201 0.2137
Peroxisomes 0.0353 0 0.006 0.0489 0.0157 0.0091 0.0264 0.0007 0.0055 0.0002 0.0001 0.0011 0.0008 0.0001 0.0034 0.0002 0.0005 0.0002
Punctate Nuclear 0.0144 0.0243 0.0424 0.0174 0.0316 0.0159 0.0228 0.0443 0.0475 0.0923 0.0802 0.0447 0.0263 0.0659 0.0865 0.1188 0.0854 0.0977
Vacuole 0.0047 0.001 0.0009 0.0023 0.0038 0.0019 0.006 0.0032 0.0035 0.0007 0.0017 0.0022 0.0029 0.0009 0.0014 0.0009 0.0023 0.0012
Vacuole Periphery 0.0011 0.0001 0.0004 0.0008 0.0014 0.0003 0.0021 0.0004 0.0014 0.0003 0.0003 0.0005 0.0005 0.0001 0.0003 0.0004 0.0007 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0919 14.995 6.5133 8.7296 20.9526 19.2181 11.0013 10.3577 10.5527 16.2915
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress
Localization
Cell Percentages nucleus (21%), cytoplasm (4%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rad54

Rad54


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad54-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available