Standard name | |
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Human Ortholog | |
Description | Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; required for regulation of copper genes in response to DNA-damaging reagents; CUP2 has a paralog, HAA1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud | 0 | 0 | – | 0 | 0 | 1 | 2 | – | 5 | 0 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 6 | 10 | 14 |
Bud Neck | 0 | 0 | – | 1 | 1 | 1 | 0 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 8 |
Bud Site | 0 | 0 | – | 0 | 0 | 5 | 4 | – | 11 | 4 | 7 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 0 | – | 27 | 38 | 35 | 78 | – | 38 | 31 | 40 | 49 | 77 | 101 | 96 | 17 | 26 | 15 | 8 | 3 | 5 | 41 | 53 | 79 |
Endoplasmic Reticulum | 0 | 0 | – | 0 | 3 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 5 | 4 | 0 | 0 | 0 | 1 | 1 | 3 |
Endosome | 1 | 0 | – | 2 | 2 | 0 | 6 | – | 1 | 0 | 1 | 3 | 3 | 18 | 16 | 5 | 1 | 4 | 2 | 1 | 0 | 0 | 10 | 9 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 4 |
Mitochondria | 12 | 7 | – | 25 | 3 | 128 | 78 | – | 175 | 170 | 206 | 221 | 4 | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 5 | 8 | 7 |
Nucleus | 38 | 6 | – | 120 | 180 | 157 | 275 | – | 197 | 271 | 219 | 341 | 89 | 84 | 159 | 84 | 63 | 43 | 78 | 40 | 29 | 108 | 128 | 104 |
Nuclear Periphery | 7 | 0 | – | 0 | 0 | 0 | 1 | – | 3 | 4 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 2 | 0 | – | 0 | 0 | 0 | 2 | – | 0 | 1 | 0 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 3 | 6 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 3 | 1 | 2 | 10 | – | 1 | 7 | 2 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 1 | 3 | 11 | 14 |
Vac/Vac Membrane | 39 | 11 | – | 20 | 31 | 16 | 44 | – | 18 | 28 | 18 | 38 | 92 | 176 | 169 | 45 | 30 | 24 | 35 | 8 | 11 | 47 | 77 | 73 |
Unique Cell Count | 84 | 18 | 175 | 243 | 258 | 440 | 325 | 403 | 363 | 520 | 222 | 323 | 384 | 140 | 113 | 79 | 138 | 63 | 52 | 227 | 319 | 338 | ||
Labelled Cell Count | 101 | 24 | 198 | 259 | 346 | 501 | 451 | 517 | 497 | 680 | 267 | 379 | 440 | 159 | 129 | 91 | 138 | 63 | 52 | 227 | 319 | 338 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.0 | – | 5.2 | 5.7 | 3.8 | 5.0 | – | 4.1 | 4.4 | 4.2 | 4.3 | 5.8 | 6.0 | 6.1 | 9.2 | 9.4 | 9.0 | 5.3 | 5.4 | 5.8 |
Std Deviation (1e-4) | 1.0 | 1.2 | – | 1.2 | 1.5 | 1.0 | 1.5 | – | 1.3 | 1.1 | 1.7 | 1.1 | 0.9 | 1.0 | 1.3 | 1.7 | 1.9 | 1.9 | 1.5 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.2653 | -0.4907 | -0.6811 | -0.6276 | -1.0416 | -0.5264 | 1.3178 | 1.1073 | 0.9574 | 0.8659 | 0.0961 | 0.7912 | 3.6277 | 2.8606 | 2.8442 | 2.7521 | 2.511 | 2.8346 |
Actin | 0.0702 | 0.0062 | 0.0053 | 0.0028 | 0.0013 | 0.0002 | 0.0426 | 0.0006 | 0.0041 | 0.0043 | 0.0003 | 0.0008 | 0.0578 | 0.0004 | 0.0002 | 0.0006 | 0.0599 | 0.0017 |
Bud | 0.0008 | 0.0005 | 0.0005 | 0.0006 | 0.0006 | 0.0001 | 0.001 | 0.0041 | 0.0015 | 0.0185 | 0.0004 | 0.0004 | 0.0008 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0003 |
Bud Neck | 0.0021 | 0.0005 | 0.0009 | 0.0004 | 0.0044 | 0.0033 | 0.0011 | 0.0022 | 0.0026 | 0.0063 | 0.0213 | 0.0083 | 0.0404 | 0.0005 | 0.0003 | 0.0005 | 0.0007 | 0.0104 |
Bud Periphery | 0.0008 | 0.0005 | 0.0005 | 0.0011 | 0.0006 | 0.0001 | 0.0015 | 0.0006 | 0.0006 | 0.005 | 0.0003 | 0.0006 | 0.0012 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0005 |
Bud Site | 0.0052 | 0.0023 | 0.0026 | 0.002 | 0.0133 | 0.0001 | 0.0055 | 0.0054 | 0.0023 | 0.0021 | 0.0208 | 0.0004 | 0.0038 | 0.0016 | 0.0005 | 0.0003 | 0.0005 | 0.0003 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0769 | 0.2994 | 0.2048 | 0.108 | 0.1915 | 0.2115 | 0.0762 | 0.2729 | 0.1427 | 0.1204 | 0.2046 | 0.1412 | 0.1307 | 0.222 | 0.1928 | 0.1728 | 0.085 | 0.1378 |
Cytoplasmic Foci | 0.0559 | 0.0046 | 0.0084 | 0.0065 | 0.0315 | 0.0067 | 0.0402 | 0.0068 | 0.0054 | 0.0223 | 0.0169 | 0.0073 | 0.011 | 0.0034 | 0.0029 | 0.0019 | 0.0046 | 0.0021 |
Eisosomes | 0.0005 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0.0017 | 0 | 0 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0059 | 0.0145 | 0.0135 | 0.0042 | 0.0018 | 0.0036 | 0.0044 | 0.0076 | 0.0052 | 0.004 | 0.0027 | 0.0031 | 0.0128 | 0.0053 | 0.003 | 0.0032 | 0.001 | 0.0021 |
Endosome | 0.0857 | 0.0109 | 0.0245 | 0.0279 | 0.0163 | 0.0184 | 0.0617 | 0.0117 | 0.0132 | 0.0247 | 0.0233 | 0.0136 | 0.027 | 0.0065 | 0.0022 | 0.0024 | 0.0012 | 0.0019 |
Golgi | 0.0596 | 0.0002 | 0.0031 | 0.012 | 0.0097 | 0.0008 | 0.0137 | 0.0011 | 0.0056 | 0.0187 | 0.006 | 0.0003 | 0.005 | 0.0005 | 0.0001 | 0.0001 | 0.0012 | 0.0001 |
Lipid Particles | 0.0287 | 0.0008 | 0.0018 | 0.0051 | 0.0147 | 0.0002 | 0.0235 | 0.0019 | 0.0022 | 0.0191 | 0.019 | 0.0003 | 0.0036 | 0.0003 | 0.0002 | 0.0003 | 0.0013 | 0.0001 |
Mitochondria | 0.0336 | 0.0015 | 0.0034 | 0.1569 | 0.0508 | 0.0006 | 0.0386 | 0.0064 | 0.0084 | 0.0683 | 0.001 | 0.001 | 0.0036 | 0.0018 | 0.0007 | 0.0011 | 0.0004 | 0.0006 |
None | 0.0473 | 0.1015 | 0.1041 | 0.0976 | 0.007 | 0.0752 | 0.0281 | 0.0932 | 0.0846 | 0.1044 | 0.0606 | 0.0521 | 0.2776 | 0.101 | 0.1607 | 0.1216 | 0.1409 | 0.0643 |
Nuclear Periphery | 0.0236 | 0.0686 | 0.0725 | 0.0419 | 0.0115 | 0.0098 | 0.0167 | 0.0339 | 0.0385 | 0.0315 | 0.0137 | 0.0198 | 0.0254 | 0.0175 | 0.0135 | 0.0129 | 0.0055 | 0.0097 |
Nucleolus | 0.0021 | 0.0021 | 0.0019 | 0.002 | 0.0116 | 0.0025 | 0.004 | 0.0027 | 0.0029 | 0.0078 | 0.0097 | 0.0042 | 0.0034 | 0.0028 | 0.0025 | 0.0049 | 0.0016 | 0.004 |
Nucleus | 0.4552 | 0.4565 | 0.5151 | 0.5053 | 0.6117 | 0.6561 | 0.5819 | 0.5215 | 0.647 | 0.4735 | 0.5581 | 0.7179 | 0.3688 | 0.6154 | 0.5982 | 0.655 | 0.6854 | 0.7453 |
Peroxisomes | 0.031 | 0.0003 | 0.0012 | 0.0057 | 0.0096 | 0.0001 | 0.0306 | 0.0041 | 0.0084 | 0.0115 | 0.0027 | 0.0004 | 0.0016 | 0.0002 | 0.0005 | 0.0001 | 0.0053 | 0.0001 |
Punctate Nuclear | 0.0074 | 0.0183 | 0.0214 | 0.0137 | 0.0037 | 0.0067 | 0.021 | 0.0147 | 0.0178 | 0.0534 | 0.0336 | 0.0244 | 0.0185 | 0.0153 | 0.0179 | 0.0185 | 0.0047 | 0.0167 |
Vacuole | 0.0046 | 0.0089 | 0.0119 | 0.0034 | 0.0072 | 0.0033 | 0.0055 | 0.0073 | 0.0056 | 0.0025 | 0.0045 | 0.0031 | 0.0034 | 0.0037 | 0.0026 | 0.0028 | 0.0004 | 0.0017 |
Vacuole Periphery | 0.0028 | 0.0017 | 0.0026 | 0.0019 | 0.0009 | 0.0007 | 0.0017 | 0.001 | 0.0012 | 0.0013 | 0.0005 | 0.0006 | 0.0013 | 0.0007 | 0.0005 | 0.0005 | 0.0001 | 0.0003 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.2744 | 11.6387 | 12.9546 | 19.2268 | 13.7153 | 14.6389 | 13.9743 | 14.8922 | 25.6529 | 17.1315 |
Translational Efficiency | 1.2996 | 1.8502 | 1.394 | 0.7197 | 1.7293 | 1.9929 | 1.2613 | 1.8594 | 1.0346 | 1.3785 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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