Standard name
Human Ortholog
Description High affinity Ca2+/Mn2+ P-type ATPase; required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting, processing; D53A mutant (Mn2+ transporting) is rapamycin sensitive, Q783A mutant (Ca2+ transporting) is rapamycin resistant; Mn2+ transport into Golgi lumen required for rapamycin sensitivity; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease; human ATP2C1 can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0.08 0.07 0.11 0.06 0.08 0.11 0.11 0.1 0.12 0 0 0 0.11 0.07 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0.06 0.05 0 0 0.05 0 0.06 0.06 0.05 0.07 0.13 0.12 0.21 0.3 0.17 0.29 0 0 0 0 0 0
Cytoplasm 0.07 0.18 0.05 0.14 0.12 0.15 0.09 0.1 0.13 0.1 0.13 0.11 0.15 0.15 0.16 0.43 0.51 0.39 0 0.07 0 0 0 0
Endoplasmic Reticulum 0.93 0.78 0.92 0.8 0.82 0.75 0.76 0.65 0.68 0.55 0.58 0.56 0.82 0.83 0.74 0 0.05 0 0.97 0.84 0.91 0.96 0.92 0.94
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.08 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0.14 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.11 0.12 0.07 0.17 0.31 0.29 0.26 0 0 0 0.09 0.15 0.17 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0 0 0 0 0 0.05 0 0.05 0.1 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.08 0.19 0.05 0.09 0.06 0.1 0 0 0 0.1 0.11 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 7 15 22 30 43 36 46 41 56 44 68 3 3 3 12 11 8 1 1 8 2 2 3
Bud 1 1 0 1 2 2 1 4 8 15 3 5 0 0 1 1 2 0 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 1 21 15 18 14 27 23 24 31 23 40 16 16 22 34 26 32 0 0 4 0 2 2
Cytoplasm 11 19 19 39 47 60 52 57 51 48 57 60 19 19 17 49 77 43 0 8 3 0 3 4
Endoplasmic Reticulum 141 84 318 229 332 302 421 359 263 268 252 316 104 106 79 3 7 3 156 96 326 164 205 279
Endosome 0 0 0 1 2 1 1 3 0 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0
Golgi 4 0 7 22 27 13 27 14 11 10 13 23 0 0 1 2 6 15 0 1 1 1 2 2
Mitochondria 0 2 5 20 6 43 65 37 66 153 128 150 1 1 0 10 23 19 0 0 3 1 2 1
Nucleus 0 0 1 1 0 0 0 0 0 0 1 1 0 0 0 1 3 2 0 0 0 0 0 0
Nuclear Periphery 5 0 21 10 6 10 24 15 20 17 22 56 1 0 0 8 2 2 2 0 3 0 0 0
Nucleolus 0 0 0 0 1 0 0 8 0 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 3 1 8 6 15 16 45 102 19 45 27 54 2 2 1 11 17 4 0 2 0 0 1 0
Unique Cell Count 151 108 347 286 404 402 554 550 388 487 437 567 127 128 107 113 150 109 161 115 358 172 223 298
Labelled Cell Count 175 115 415 366 486 504 699 668 503 648 571 774 146 147 124 131 176 128 161 115 358 172 223 298


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 19.3 19.3 15.9 16.6 17.2 14.4 13.5 14.0 13.1 12.4 12.4 12.4 20.6 20.6 21.7 11.1 12.9 16.8 22.2 22.7 23.7
Std Deviation (1e-4) 3.7 4.6 3.1 3.3 2.8 2.4 2.4 2.3 2.3 2.3 2.4 2.4 4.5 4.5 5.0 2.4 3.5 4.0 4.1 4.4 4.2
Intensity Change (Log2) – – – 0.06 0.11 -0.14 -0.23 -0.18 -0.28 -0.36 -0.36 -0.36 0.37 0.37 0.45 -0.51 -0.3 0.08 0.48 0.51 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.8 1.8 3.3 1.4 2.3 3.2 3.7 3.0 3.9 0 0 0 2.5 1.4 1.3
Bud 0 0 0 0 0 0 3.3 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 -1.0 -1.7 -0.8 -1.3 0.1 0.2 -0.5 0.6 2.4 2.3 4.5 6.8 3.9 6.6
Cytoplasm 3.5 3.0 4.2 2.1 2.6 3.5 2.3 3.6 2.7 3.4 3.3 3.5 9.9 11.9 9.0
Endoplasmic Reticulum -4.2 -3.8 -6.0 -6.0 -8.9 -7.9 -11.4 -10.6 -11.4 -3.0 -2.8 -4.9 -17.9 -18.7 -17.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.4 3.1 1.0 2.2 0.5 0.7 0 0.8 1.7 0 0 0 0 0 5.0
Mitochondria 3.6 0.1 5.2 5.6 3.6 7.1 10.9 10.3 9.8 0 0 0 0 6.2 6.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.5 -3.4 -2.4 -1.2 -2.5 -0.5 -1.7 -0.6 2.0 -2.4 -2.8 0 0.4 -2.3 -1.8
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.2 1.1 1.3 3.6 7.2 1.9 4.0 2.6 4.2 0 0 0 0 4.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.1363 21.0002 19.7746 18.8744 15.9887 20.306 22.9327 23.4748 23.9014 21.1275 23.4819 21.9377 9.7478 23.3258 18.274 20.4936 24.4819 20.3439
Actin 0.0416 0.0039 0.02 0.0523 0.0684 0.0009 0.0117 0.0375 0.0011 0.0052 0 0.0035 0.311 0 0.0438 0.0001 0.0006 0.0109
Bud 0.0009 0.0001 0.0039 0.0003 0.003 0.0028 0.0003 0.002 0.0001 0.0001 0 0.0002 0.0012 0 0.0005 0 0 0.0001
Bud Neck 0.0057 0.0005 0.0015 0.0012 0.0024 0.0005 0.0005 0.0003 0.0002 0.0006 0.0001 0.0003 0.0018 0 0.0356 0.0003 0.0004 0.0012
Bud Periphery 0.0017 0.0002 0.0053 0.0004 0.0058 0.0048 0.0002 0.0026 0.0002 0.0005 0 0.0002 0.0015 0 0.0007 0.0001 0 0.0002
Bud Site 0.0091 0.0007 0.0017 0.0017 0.0081 0.0002 0.0078 0.0141 0.0008 0.0001 0 0.0003 0.0028 0 0.0055 0 0 0.0001
Cell Periphery 0.0026 0.0042 0.0035 0.0016 0.0004 0.0003 0.0007 0.0023 0.0013 0.0025 0.0002 0.0003 0.0009 0.0011 0.0021 0.0013 0.0004 0.0003
Cytoplasm 0.0166 0.0911 0.0712 0.0294 0.0574 0.0119 0.0209 0.0018 0.0028 0.0057 0.0015 0.0026 0.0015 0.005 0.015 0.0032 0.0021 0.0025
Cytoplasmic Foci 0.0179 0.0023 0.0015 0.015 0.022 0.0002 0.0422 0.0011 0.0052 0.0006 0.0001 0.0018 0.0011 0.0001 0.0165 0.0001 0.0001 0.0002
Eisosomes 0.0011 0.0001 0.0002 0.0003 0.0003 0 0.0001 0.0005 0.0001 0.0003 0 0 0.0005 0 0.0001 0 0.0001 0.0002
Endoplasmic Reticulum 0.7283 0.8592 0.8235 0.8326 0.7108 0.9583 0.7852 0.8608 0.9231 0.9231 0.9918 0.9335 0.3692 0.9839 0.804 0.9823 0.9522 0.9196
Endosome 0.0301 0.0052 0.0062 0.0097 0.024 0.0015 0.0204 0.005 0.0106 0.0166 0.0008 0.0193 0.0041 0.0006 0.0169 0.0013 0.0022 0.0039
Golgi 0.0101 0.0042 0.0027 0.005 0.0112 0.0003 0.0058 0.0256 0.0046 0.0207 0.0007 0.0087 0.0076 0.0001 0.0073 0.0006 0.0049 0.0056
Lipid Particles 0.0196 0.0064 0.0033 0.0115 0.0163 0.0002 0.0133 0.0122 0.0057 0.0003 0.0001 0.0012 0.0011 0.0003 0.0035 0.0004 0 0.0002
Mitochondria 0.0115 0.0002 0.0147 0.0016 0.0139 0.0006 0.0012 0.0154 0.0063 0.0035 0 0.0074 0.0015 0 0.0028 0.0001 0.0003 0.0138
None 0.0158 0.0001 0.0028 0.0074 0.008 0.0001 0.004 0.0002 0.0006 0.0002 0 0.0002 0.0003 0 0.0013 0 0.0001 0
Nuclear Periphery 0.0453 0.0142 0.0223 0.0155 0.0314 0.0132 0.0232 0.0137 0.0149 0.014 0.0039 0.0165 0.2707 0.007 0.0197 0.0074 0.033 0.034
Nucleolus 0.0072 0.0001 0.0033 0.0004 0.0006 0.0001 0.0006 0.0001 0.0016 0 0 0 0.0001 0 0.0005 0 0 0
Nucleus 0.0066 0.001 0.0033 0.0032 0.004 0.0028 0.0059 0.0006 0.0019 0.0007 0.0001 0.0009 0.0038 0.0002 0.009 0.0002 0.001 0.0008
Peroxisomes 0.0049 0 0.0008 0.0028 0.0027 0 0.0386 0.0009 0.0005 0 0 0.0002 0.0004 0 0.0046 0 0 0.0002
Punctate Nuclear 0.0141 0.0003 0.0019 0.0045 0.0043 0 0.0134 0.0001 0.0121 0 0 0.0003 0.0003 0 0.0061 0 0 0
Vacuole 0.0072 0.0057 0.0042 0.0029 0.0026 0.0011 0.0029 0.0024 0.0041 0.0035 0.0005 0.0016 0.0025 0.0016 0.0033 0.002 0.001 0.0034
Vacuole Periphery 0.002 0.0004 0.002 0.0007 0.0026 0.0003 0.001 0.001 0.002 0.0017 0.0001 0.0012 0.0161 0.0001 0.0011 0.0007 0.0015 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 109.0642 78.1357 74.1426 90.6357 72.4583 85.0384 91.3252 85.0551 94.1534 105.4609
Translational Efficiency 0.6834 0.6557 0.7341 0.6232 0.6233 0.8326 0.5812 0.5641 0.5255 0.5078

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description High affinity Ca2+/Mn2+ P-type ATPase; required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting, processing; D53A mutant (Mn2+ transporting) is rapamycin sensitive, Q783A mutant (Ca2+ transporting) is rapamycin resistant; Mn2+ transport into Golgi lumen required for rapamycin sensitivity; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease; human ATP2C1 can complement yeast null mutant
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Pmr1

Pmr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pmr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available