Standard name
Human Ortholog
Description Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.33 0.22 0.62 0.31 0.18 0.29 0.29 0.28 0.33 0.26 0.27 0.26 0.24 0.13 0.13 0.5 0.39 0.36 0.73 0.59 0.79 0.57 0.51 0.45
Bud 0.09 0.06 0.14 0.09 0.09 0.07 0.1 0.11 0.11 0.07 0.09 0.08 0 0 0 0.1 0.1 0.07 0 0.05 0 0.08 0.07 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.1 0.09
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.1 0.16 0.11 0 0.06 0.09 0.09 0.09 0.07 0.06 0.09 0.11 0.14 0.11 0.12 0.1 0.14 0 0 0 0 0 0
Cytoplasm 0.44 0.38 0.19 0.4 0.57 0.33 0.26 0.22 0.18 0.19 0.16 0.22 0.57 0.65 0.72 0.14 0.2 0.19 0.09 0.11 0.08 0.09 0.16 0.22
Endoplasmic Reticulum 0.24 0.33 0.24 0.29 0.18 0.25 0.26 0.26 0.28 0.24 0.24 0.3 0.24 0.21 0.18 0.07 0.08 0 0 0.08 0 0.05 0 0
Endosome 0 0 0 0 0.06 0.05 0.06 0.09 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.4 0.31 0.31 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.16 0.24 0.15 0.26 0.3 0.34 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0.09 0.05 0.09 0.08 0.07 0 0 0 0 0.1 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 28 15 142 58 48 124 160 130 145 138 111 121 46 29 41 44 23 21 80 63 202 27 96 105
Bud 8 4 32 17 23 28 57 49 48 36 38 36 6 6 6 9 6 4 2 5 3 3 12 16
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 7 6 2 19 20
Bud Site 0 0 1 4 4 5 10 4 4 4 1 2 0 0 0 1 0 1 – – – – – –
Cell Periphery 2 7 37 20 8 26 52 44 40 38 26 44 20 33 36 11 6 8 0 0 3 0 1 2
Cytoplasm 37 26 44 74 152 140 144 103 79 99 65 103 107 148 232 12 12 11 9 11 19 4 30 51
Endoplasmic Reticulum 20 23 56 55 47 106 143 120 125 124 97 139 46 47 58 6 5 2 3 8 1 2 6 7
Endosome 1 0 4 2 16 23 31 40 14 16 20 5 0 1 0 0 0 2 3 4 6 1 11 8
Golgi 1 1 4 2 0 4 6 16 16 9 5 8 0 1 2 35 18 18 0 0 2 0 0 1
Mitochondria 7 1 4 8 6 68 132 70 118 160 142 123 0 0 0 3 1 0 2 2 0 1 2 9
Nucleus 0 0 0 0 2 1 4 6 4 2 1 3 0 2 3 0 1 0 0 0 0 0 2 0
Nuclear Periphery 0 0 0 0 0 2 2 1 0 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 1 3 5 0 5 2 2 0 0 0 2 1 1 0 0 0 0 0 0
Peroxisomes 0 0 8 4 1 8 15 17 4 11 6 3 0 0 0 1 1 1 1 0 5 0 0 0
SpindlePole 0 0 5 0 0 4 7 4 2 2 0 2 3 0 2 4 4 1 0 0 1 0 0 0
Vac/Vac Membrane 0 0 3 6 13 17 19 41 21 48 33 35 1 1 1 3 6 9 1 1 1 1 4 4
Unique Cell Count 85 69 229 187 267 425 558 465 446 526 412 471 189 228 321 88 59 59 110 108 256 48 190 233
Labelled Cell Count 104 77 341 250 320 557 785 650 620 694 548 628 229 268 381 131 84 79 110 108 256 48 190 233


Actin, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 10.1 9.8 8.9 9.0 8.7 8.6 9.0 9.0 8.9 8.8 9.1 10.7 11.1 11.5 18.8 18.3 17.3 – 10.1 10.1
Std Deviation (1e-4) 1.3 1.4 1.2 1.3 1.6 1.6 1.6 1.7 1.6 1.9 2.0 2.1 1.2 1.5 1.5 3.6 2.8 3.5 – 1.4 1.7
Intensity Change (Log2) – – – -0.14 -0.13 -0.18 -0.19 -0.13 -0.13 -0.14 -0.15 -0.11 0.13 0.18 0.23 0.93 0.89 0.82 – 0.04 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -6.3 -10.1 -8.2 -8.7 -8.6 -7.3 -9.4 -8.7 -9.3 -7.7 -10.9 -12.1 -1.9 -3.2 -3.7
Bud -1.5 -1.9 -3.1 -1.5 -1.3 -1.2 -3.1 -1.8 -2.7 -3.8 -4.4 -5.5 -0.9 -0.8 -1.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.6 -5.1 -4.2 -2.8 -2.6 -2.8 -3.8 -4.0 -2.6 -1.7 -0.5 -1.7 -0.8 -1.2 -0.5
Cytoplasm 4.6 8.6 3.7 2.0 0.9 -0.5 -0.1 -1.1 0.8 7.9 9.9 12.3 -1.2 0.2 -0.1
Endoplasmic Reticulum 1.1 -1.9 0.1 0.3 0.4 1.0 -0.3 -0.3 1.4 0 -1.0 -1.8 -3.5 -2.7 -3.6
Endosome 0 2.4 2.2 2.4 3.5 1.1 1.0 2.0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1.3 1.3 0 0 0 0 0 0 9.2 0 0
Mitochondria 1.5 0 5.6 7.4 5.3 7.9 8.8 9.5 7.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -0.8 0 -1.3 -0.6 0.1 0 -1.1 -1.7 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.2 1.9 1.6 3.8 2.3 3.9 3.5 3.4 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.2826 9.9264 9.0768 8.2828 7.6976 10.1124 6.5894 8.6221 7.8399 6.5571 4.9974 8.511 6.5536 8.301 7.3311 7.4145 6.7785 7.7319
Actin 0.6918 0.5972 0.6385 0.4937 0.5368 0.6516 0.7733 0.6051 0.7217 0.5317 0.6981 0.7245 0.8013 0.6845 0.7426 0.6633 0.6395 0.7121
Bud 0.0022 0.0035 0.0149 0.0219 0.0064 0.0015 0.0017 0.0047 0.0136 0.0095 0.0053 0.001 0.0024 0.0028 0.0082 0.0344 0.0107 0.0019
Bud Neck 0.0136 0.0052 0.0204 0.0026 0.0053 0.1445 0.0101 0.0103 0.0184 0.038 0.0345 0.1715 0.0052 0.0063 0.0207 0.0092 0.0184 0.1516
Bud Periphery 0.0024 0.0021 0.013 0.0301 0.0107 0.0017 0.0023 0.0022 0.0096 0.0101 0.0047 0.0012 0.0019 0.0011 0.0065 0.0483 0.0094 0.0017
Bud Site 0.0174 0.0651 0.0315 0.0033 0.014 0.0018 0.0157 0.1367 0.0101 0.0038 0.004 0.0012 0.0193 0.0714 0.0285 0.0022 0.0123 0.0037
Cell Periphery 0.001 0.0019 0.0014 0.0011 0.0005 0.0007 0.0005 0.001 0.0004 0.0007 0.0006 0.0005 0.0004 0.0009 0.0004 0.0005 0.0008 0.0004
Cytoplasm 0.065 0.2487 0.1601 0.2676 0.2084 0.1085 0.075 0.2088 0.1604 0.302 0.1126 0.0611 0.0707 0.1989 0.1421 0.1569 0.2616 0.0891
Cytoplasmic Foci 0.062 0.0279 0.0337 0.0362 0.0752 0.0158 0.0237 0.0146 0.0193 0.0084 0.0298 0.0083 0.0201 0.0128 0.0113 0.0516 0.0118 0.0178
Eisosomes 0.0007 0.0005 0.0004 0.0002 0.0003 0.0005 0.0006 0.0003 0.0002 0.0004 0.0004 0.0004 0.0005 0.0002 0.0002 0.0001 0.0002 0.0004
Endoplasmic Reticulum 0.0054 0.006 0.0074 0.0037 0.0025 0.0044 0.004 0.0014 0.0024 0.002 0.003 0.001 0.0039 0.001 0.0023 0.0016 0.0041 0.0019
Endosome 0.0297 0.0038 0.0132 0.0121 0.042 0.0102 0.019 0.0007 0.018 0.0233 0.0148 0.0076 0.0173 0.0011 0.0072 0.003 0.0065 0.0029
Golgi 0.0214 0.0082 0.0166 0.0238 0.0486 0.0291 0.0141 0.0035 0.0052 0.0109 0.021 0.0055 0.0107 0.0022 0.0043 0.0041 0.0057 0.0046
Lipid Particles 0.0187 0.0009 0.0029 0.0134 0.004 0.0018 0.019 0.0003 0.0009 0.0042 0.0307 0.0058 0.0062 0.0001 0.0018 0.0086 0.0016 0.0004
Mitochondria 0.0132 0.001 0.0123 0.0172 0.0126 0.0159 0.0128 0.0005 0.0017 0.0186 0.01 0.0013 0.0026 0.0004 0.0096 0.0012 0.0028 0.0006
None 0.007 0.0013 0.0021 0.0023 0.0026 0.0005 0.0066 0.0006 0.0009 0.0227 0.0008 0.0003 0.0075 0.0005 0.0009 0.0004 0.0009 0.0003
Nuclear Periphery 0.0028 0.0006 0.0016 0.0011 0.0015 0.0006 0.0035 0.0004 0.0038 0.0017 0.0043 0.0013 0.0079 0.0007 0.0015 0.0009 0.002 0.0007
Nucleolus 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0008 0 0.0001 0.0003 0.0002 0.0002 0.0002 0 0 0.0001 0.0009 0
Nucleus 0.0017 0.0014 0.0014 0.0019 0.0037 0.0008 0.0018 0.0009 0.0014 0.0016 0.0013 0.0012 0.0031 0.0021 0.0016 0.0011 0.003 0.001
Peroxisomes 0.0378 0.023 0.0254 0.066 0.0208 0.0086 0.0105 0.0071 0.0078 0.0029 0.0191 0.0032 0.0139 0.0117 0.008 0.0092 0.0041 0.0075
Punctate Nuclear 0.0049 0.0013 0.0015 0.0011 0.0015 0.0009 0.0032 0.0008 0.0017 0.0004 0.0035 0.0011 0.0038 0.0009 0.0017 0.003 0.0018 0.0011
Vacuole 0.0009 0.0002 0.0012 0.0005 0.0015 0.0004 0.001 0.0001 0.0016 0.0045 0.0006 0.0015 0.0006 0.0001 0.0004 0.0002 0.0014 0.0002
Vacuole Periphery 0.0004 0.0001 0.0004 0.0002 0.0007 0.0004 0.0006 0 0.0009 0.0024 0.0006 0.0003 0.0006 0 0.0002 0.0001 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.6981 15.7356 19.6657 31.7952 26.4261 23.1485 32.8673 44.9316 41.45 34.2814
Translational Efficiency 1.9823 4.1397 2.6702 1.2295 1.407 3.2337 2.2881 1.6918 1.7525 1.5003

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations
Localization
Cell Percentages actin (91%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gts1

Gts1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gts1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available