Standard name
Human Ortholog
Description Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.06 0 0 0.06 0 0 0 0 0.09 0.15 0.2 0.13 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.13 0.07 0.1 0.13 0.25 0.33 0.28 0.29 0.35 0.46 0.41 0 0 0 0 0.09 0 0 0 0 0 0 0
Nucleus 0.87 0.83 0.84 0.83 0.8 0.72 0.62 0.62 0.62 0.59 0.55 0.58 0.85 0.79 0.73 0.73 0.65 0.64 0.67 0.72 0.63 0.74 0.69 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.29 0.14 0.14 0.15 0.23 0.32 0.32 0.36 0.41 0.43 0.41 0.08 0 0.05 0.06 0.12 0.06 0.22 0.22 0.24 0.13 0.16 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.16 0 0 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 1
Bud 0 1 0 0 1 1 9 3 4 7 7 5 0 0 0 1 0 1 3 0 4 6 6 6
Bud Neck 0 0 0 0 0 0 1 1 3 3 2 0 0 0 0 0 0 0 1 0 0 1 3 1
Bud Site 0 0 1 1 1 9 15 16 17 23 19 23 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 9 3 11 16 16 18 28 31 21 15 17 8 44 85 118 13 8 9 0 0 2 6 3 3
Endoplasmic Reticulum 0 0 4 0 2 0 0 1 0 0 0 0 1 1 1 1 2 3 0 0 2 0 0 2
Endosome 4 0 0 2 0 1 0 4 1 0 0 0 5 6 19 1 4 3 9 2 3 6 15 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2 4 0 2 0 0 2
Mitochondria 46 19 23 21 34 114 209 148 149 215 224 228 0 1 1 4 8 2 2 0 1 2 5 8
Nucleus 419 121 259 183 207 324 391 331 323 369 271 325 402 440 443 73 58 61 340 111 221 339 399 381
Nuclear Periphery 0 0 4 1 0 2 3 4 2 3 2 2 1 0 1 0 0 2 0 0 1 1 1 4
Nucleolus 100 42 44 32 39 101 200 170 188 256 212 229 38 24 30 6 11 6 114 33 84 58 93 108
Peroxisomes 1 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0 1 0 0 0 0 0
SpindlePole 2 0 2 0 0 1 1 0 2 1 2 1 4 8 10 1 2 1 5 0 9 16 13 18
Vac/Vac Membrane 8 6 4 7 3 5 9 6 5 7 9 10 6 14 19 8 11 15 18 3 7 14 28 44
Unique Cell Count 482 145 310 221 260 448 626 534 521 622 489 562 471 560 604 100 89 95 508 154 350 458 578 604
Labelled Cell Count 589 192 352 263 303 576 866 715 715 899 767 831 502 583 645 108 104 106 508 154 350 458 578 604


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 7.4 6.7 6.3 6.4 5.8 5.7 5.6 5.8 5.7 5.4 5.4 8.5 8.5 8.0 8.6 8.4 8.6 8.2 8.0 8.4
Std Deviation (1e-4) 1.0 1.3 1.3 1.3 1.7 1.5 1.4 1.5 1.4 1.3 1.5 1.4 1.4 1.5 1.7 1.8 1.6 2.0 1.4 1.3 1.6
Intensity Change (Log2) – – – -0.11 -0.06 -0.21 -0.24 -0.26 -0.23 -0.25 -0.33 -0.31 0.33 0.33 0.24 0.35 0.32 0.36 0.28 0.25 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2.3 2.7 2.8 3.1 3.1 3.3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 1.5 0.3 0.7 1.5 0.3 -1.0 -0.1 -2.1 3.1 5.2 6.6 3.5 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 2.2 6.3 8.7 7.1 7.3 9.0 11.4 10.3 -6.0 -6.2 -6.5 -1.2 0.5 -1.9
Nucleus -0.2 -1.2 -3.6 -6.6 -6.6 -6.6 -7.4 -8.2 -7.7 0.7 -1.8 -3.5 -2.3 -3.8 -4.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.3 2.9 5.8 5.7 6.8 8.3 8.6 8.1 -2.7 -5.2 -4.8 -2.2 -0.4 -2.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0.6 0 0 1.2 1.7 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0688 5.6094 5.1505 5.0415 4.8283 5.511 4.6749 4.6466 4.4501 4.0903 3.865 4.4504 4.2414 4.2135 4.0375 4.0922 3.5714 3.9773
Actin 0.0012 0 0.0003 0.0001 0.0009 0 0.0064 0 0.0118 0.0012 0.01 0.0006 0.0115 0 0.0008 0 0.0441 0
Bud 0.0004 0 0 0.0001 0.0001 0.0001 0.0008 0 0.0002 0.0003 0.0011 0.0001 0.0004 0 0.0001 0.0002 0.001 0.0001
Bud Neck 0.0008 0.0002 0.0001 0.0012 0.0022 0.0026 0.0045 0.0001 0.0021 0.0005 0.0184 0.002 0.0017 0.0003 0.0004 0.0004 0.0024 0.0018
Bud Periphery 0.0008 0 0 0.0001 0.0002 0.0001 0.002 0 0.0004 0.0003 0.0027 0.0003 0.0007 0 0.0001 0.0002 0.0038 0.0001
Bud Site 0.0005 0.0003 0.0001 0.0001 0.0005 0.0002 0.0073 0.0001 0.001 0.0019 0.0132 0.0003 0.002 0.0002 0.0002 0.0003 0.0015 0.0002
Cell Periphery 0.0002 0 0 0 0.0001 0.0001 0.0014 0 0.0001 0.0001 0.0014 0 0.0001 0 0 0 0.0005 0
Cytoplasm 0.0067 0.014 0.0001 0.0011 0.0014 0.0001 0.0043 0.0001 0.0017 0.0046 0.0034 0.0004 0.0012 0.0001 0.0002 0.0001 0.0027 0
Cytoplasmic Foci 0.0004 0.0001 0.0002 0 0.0006 0 0.0071 0 0.0076 0.0039 0.03 0.0011 0.004 0 0.0111 0 0.0047 0
Eisosomes 0.0001 0 0 0 0.0001 0 0.0002 0 0.0001 0 0.0003 0 0.0001 0 0 0 0.0008 0
Endoplasmic Reticulum 0.0012 0.0004 0.0004 0.0002 0.0002 0 0.0059 0 0.0011 0.0031 0.0034 0.0002 0.0007 0 0.0001 0 0.0045 0
Endosome 0.0015 0.0008 0.0049 0.0005 0.0008 0 0.0249 0 0.0081 0.0321 0.0294 0.0016 0.0073 0 0.0014 0 0.0174 0
Golgi 0.0003 0 0.0005 0 0.0001 0 0.0058 0 0.0019 0.002 0.02 0.0015 0.0027 0 0.0006 0 0.0061 0
Lipid Particles 0.0003 0 0.0014 0 0.0005 0 0.0148 0 0.0039 0.0037 0.024 0.0027 0.0123 0 0.0072 0 0.0029 0
Mitochondria 0.0211 0.0002 0.0006 0.0003 0.0006 0.0001 0.0088 0 0.0013 0.0012 0.0526 0.0008 0.0033 0.0001 0.0003 0.0002 0.0049 0.0001
None 0.0141 0.0001 0.0002 0.0003 0.0262 0 0.0017 0 0.0007 0.0012 0.0021 0.0001 0.0003 0 0.0008 0 0.0062 0
Nuclear Periphery 0.0041 0.0041 0.0037 0.0057 0.01 0.0004 0.0173 0.0004 0.0108 0.0207 0.0344 0.0014 0.0056 0.0009 0.0007 0.0004 0.0183 0.0002
Nucleolus 0.0347 0.0231 0.0187 0.0185 0.1818 0.0845 0.0223 0.0184 0.0153 0.0104 0.1189 0.0486 0.0305 0.0277 0.0252 0.0167 0.1126 0.069
Nucleus 0.9022 0.9512 0.9672 0.9661 0.7524 0.909 0.8383 0.9799 0.9188 0.8989 0.5568 0.9324 0.9019 0.9695 0.9435 0.9802 0.7491 0.9274
Peroxisomes 0.0002 0 0.0001 0 0.0001 0 0.0062 0 0.0027 0.0006 0.0127 0.0016 0.0043 0 0.0059 0 0.0013 0
Punctate Nuclear 0.0064 0.0045 0.0008 0.0051 0.0208 0.0024 0.0064 0.0009 0.0081 0.0077 0.0479 0.0039 0.0073 0.0009 0.0012 0.0013 0.009 0.0007
Vacuole 0.0017 0.0008 0.0003 0.0004 0.0003 0.0003 0.0106 0 0.0016 0.0044 0.0121 0.0003 0.0017 0.0001 0.0002 0 0.0038 0.0001
Vacuole Periphery 0.0014 0.0001 0.0004 0.0001 0.0002 0 0.0031 0 0.0007 0.0012 0.0051 0.0001 0.0005 0 0.0001 0 0.0025 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4266 15.9678 13.4646 19.2208 14.7377 13.7951 18.8971 16.8405 18.0983 20.7628
Translational Efficiency 0.811 0.7753 0.9486 0.5756 0.8316 1.0004 0.9215 0.7839 0.8397 0.6755

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Hos2

Hos2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hos2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available