Standard name | |
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Human Ortholog | |
Description | Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain; MDS3 has a paralog, PMD1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 3 | 1 | 0 | 1 | 3 |
Bud | 0 | 2 | 4 | 1 | 5 | 3 | 2 | 10 | 9 | 1 | 2 | 4 | 0 | 0 | 13 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 4 | 3 | 3 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 28 | 6 | 2 | 2 | 4 | 7 | 5 | 6 | 4 | 8 | 7 | 1 | 5 | 16 | 9 | – | 26 | 19 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 345 | 367 | 160 | 119 | 253 | 249 | 290 | 295 | 180 | 220 | 89 | 119 | 194 | 309 | 341 | – | 110 | 97 | 326 | 354 | 144 | 132 | 263 | 284 |
Endoplasmic Reticulum | 1 | 8 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | – | 4 | 2 | 7 | 4 | 2 | 0 | 1 | 4 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 4 | 2 | 0 | 0 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 1 |
Mitochondria | 1 | 3 | 6 | 11 | 11 | 40 | 64 | 87 | 73 | 90 | 44 | 83 | 0 | 1 | 7 | – | 3 | 3 | 1 | 3 | 5 | 0 | 0 | 5 |
Nucleus | 0 | 2 | 2 | 1 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 2 | 1 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 1 |
Unique Cell Count | 350 | 380 | 165 | 123 | 258 | 257 | 313 | 328 | 212 | 251 | 117 | 169 | 195 | 310 | 344 | 117 | 101 | 349 | 386 | 166 | 140 | 275 | 314 | |
Labelled Cell Count | 379 | 390 | 177 | 136 | 275 | 302 | 365 | 409 | 271 | 325 | 146 | 211 | 202 | 327 | 370 | 145 | 125 | 349 | 386 | 166 | 140 | 275 | 314 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 4.6 | 4.2 | 4.6 | 4.4 | 3.7 | 3.6 | 3.8 | 3.6 | 3.3 | 3.6 | 3.5 | 5.4 | 5.6 | 4.9 | – | 7.6 | 8.1 | 5.7 | 5.0 | 6.0 |
Std Deviation (1e-4) | 0.9 | 0.7 | 1.2 | 2.5 | 1.8 | 1.6 | 1.4 | 1.7 | 2.4 | 1.3 | 2.0 | 1.7 | 0.9 | 1.0 | 1.1 | – | 1.6 | 1.9 | 1.6 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.07 | -0.16 | -0.2 | -0.11 | -0.2 | -0.35 | -0.2 | -0.27 | 0.38 | 0.42 | 0.23 | – | 0.87 | 0.96 | 0.46 | 0.26 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5701 | 1.1928 | 0.8735 | 0.5285 | 1.3033 | 1.2299 | 2.0674 | 2.5974 | 2.2021 | 1.8055 | 2.066 | 2.3255 | 1.0458 | 1.5463 | 1.2275 | 1.1526 | 1.262 | 1.1465 |
Actin | 0.0514 | 0.0015 | 0.01 | 0.0004 | 0.103 | 0.0005 | 0.0603 | 0.0004 | 0.0043 | 0.0111 | 0.0124 | 0.0089 | 0.0413 | 0.0007 | 0.0014 | 0.0014 | 0.0218 | 0.0102 |
Bud | 0.0034 | 0.0002 | 0.0002 | 0.0001 | 0.0013 | 0.0029 | 0.0018 | 0.0032 | 0.0013 | 0.0018 | 0.0002 | 0.0035 | 0.001 | 0.0003 | 0.0023 | 0.0023 | 0.0005 | 0.0003 |
Bud Neck | 0.0018 | 0.0008 | 0.0007 | 0.0004 | 0.0029 | 0.0011 | 0.0197 | 0.0003 | 0.0005 | 0.0012 | 0.0002 | 0.0025 | 0.0004 | 0.0007 | 0.0028 | 0.0003 | 0.0005 | 0.0066 |
Bud Periphery | 0.0073 | 0.0006 | 0.0004 | 0.0002 | 0.0028 | 0.0024 | 0.0036 | 0.0011 | 0.004 | 0.0023 | 0.0003 | 0.0055 | 0.0017 | 0.0004 | 0.0044 | 0.0044 | 0.0008 | 0.0005 |
Bud Site | 0.0052 | 0.0202 | 0.0021 | 0.0003 | 0.0058 | 0.0002 | 0.0257 | 0.0101 | 0.0079 | 0.0102 | 0.0003 | 0.0026 | 0.0098 | 0.0319 | 0.0063 | 0.0011 | 0.0009 | 0.0005 |
Cell Periphery | 0.0039 | 0.0071 | 0.004 | 0.0008 | 0.002 | 0.001 | 0.0014 | 0.0006 | 0.001 | 0.0003 | 0.0001 | 0.0002 | 0.0044 | 0.0021 | 0.002 | 0.0009 | 0.0005 | 0.0006 |
Cytoplasm | 0.5452 | 0.7997 | 0.6651 | 0.6243 | 0.5958 | 0.7487 | 0.399 | 0.7067 | 0.6713 | 0.6432 | 0.6167 | 0.6985 | 0.3859 | 0.6336 | 0.5243 | 0.5286 | 0.5498 | 0.4821 |
Cytoplasmic Foci | 0.0329 | 0.003 | 0.0119 | 0.0028 | 0.003 | 0.0029 | 0.0365 | 0.0049 | 0.0113 | 0.0109 | 0.0074 | 0.0045 | 0.0184 | 0.0029 | 0.0256 | 0.0058 | 0.0105 | 0.0071 |
Eisosomes | 0.0012 | 0.0004 | 0.0014 | 0.0002 | 0.0009 | 0.0001 | 0.0009 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0082 | 0.0056 | 0.0042 | 0.0016 | 0.0081 | 0.0031 | 0.0052 | 0.0017 | 0.0021 | 0.0023 | 0.002 | 0.0017 | 0.0096 | 0.0012 | 0.0024 | 0.0017 | 0.0027 | 0.0014 |
Endosome | 0.0231 | 0.0008 | 0.003 | 0.0008 | 0.0289 | 0.0006 | 0.0361 | 0.0012 | 0.0074 | 0.0097 | 0.0066 | 0.0105 | 0.0222 | 0.0004 | 0.0048 | 0.0016 | 0.004 | 0.0013 |
Golgi | 0.0118 | 0.0003 | 0.0014 | 0.0002 | 0.0247 | 0.0001 | 0.0189 | 0.0002 | 0.0017 | 0.0048 | 0.0157 | 0.0062 | 0.0076 | 0.0001 | 0.002 | 0.0003 | 0.0011 | 0.001 |
Lipid Particles | 0.0085 | 0.0005 | 0.006 | 0.0002 | 0.0025 | 0.0002 | 0.0176 | 0.0002 | 0.0035 | 0.0006 | 0.0363 | 0.0011 | 0.0075 | 0.0001 | 0.0012 | 0.0002 | 0.0008 | 0.0011 |
Mitochondria | 0.0056 | 0.0001 | 0.0116 | 0.0002 | 0.0104 | 0.0001 | 0.0095 | 0.0002 | 0.0011 | 0.0017 | 0.025 | 0.0074 | 0.0044 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0004 |
None | 0.2551 | 0.1564 | 0.2568 | 0.3654 | 0.1999 | 0.2342 | 0.3036 | 0.2647 | 0.2721 | 0.2933 | 0.2504 | 0.2402 | 0.4658 | 0.3232 | 0.4108 | 0.447 | 0.4013 | 0.4824 |
Nuclear Periphery | 0.0028 | 0.0003 | 0.0003 | 0.0002 | 0.0024 | 0.0003 | 0.0157 | 0.0002 | 0.0004 | 0.0006 | 0.0003 | 0.0023 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0005 | 0.0002 |
Nucleolus | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.002 | 0.0003 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0011 | 0.0005 | 0.0005 | 0.0004 | 0.0005 | 0.0005 | 0.0033 | 0.0014 | 0.0007 | 0.0007 | 0.0004 | 0.0007 | 0.0003 | 0.0005 | 0.0007 | 0.0006 | 0.0006 | 0.0003 |
Peroxisomes | 0.0182 | 0 | 0.0177 | 0.0001 | 0.0006 | 0 | 0.0107 | 0.0001 | 0.0037 | 0.0017 | 0.0238 | 0.0014 | 0.0159 | 0 | 0.0047 | 0.0001 | 0.0007 | 0.0023 |
Punctate Nuclear | 0.0071 | 0.0002 | 0.0012 | 0.0002 | 0.0003 | 0.0002 | 0.0225 | 0.001 | 0.0008 | 0.0005 | 0.0004 | 0.0005 | 0.0005 | 0.0002 | 0.0015 | 0.0002 | 0.0005 | 0.0004 |
Vacuole | 0.0044 | 0.0014 | 0.0011 | 0.0009 | 0.0026 | 0.0009 | 0.0032 | 0.0015 | 0.0037 | 0.0025 | 0.0009 | 0.0012 | 0.002 | 0.0008 | 0.0015 | 0.0019 | 0.0017 | 0.0007 |
Vacuole Periphery | 0.0012 | 0 | 0.0001 | 0.0001 | 0.0017 | 0.0001 | 0.0027 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.5943 | 19.6657 | 21.9711 | 30.6898 | 23.2346 | 27.9157 | 29.9934 | 30.158 | 35.1445 | 33.6153 |
Translational Efficiency | 0.4131 | 0.4011 | 0.4063 | 0.357 | 0.3691 | 0.4286 | 0.3096 | 0.3499 | 0.3138 | 0.342 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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