Standard name
Human Ortholog
Description Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; specifically required for diauxic shift-induced H2B deposition onto rDNA genes; mutations cause reduced nucleosome occupancy over highly transcribed regions; coregulates transcription with Mot1p through preinitiation complex assembly and nucleosome organization
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.06 0.06 0.05 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.08 – 0.11 0.05 0 0 0 0 0.07 0.13 0 0 0 0 0 0
Nucleus 0.92 0.94 0.94 0.96 0.92 0.88 0.81 0.8 0.8 – 0.69 0.79 0.94 0.9 0.83 0.87 0.84 0.83 0.85 0.91 0.89 0.87 0.78 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 0.3 0.5 0.19 0.16 0.34 0.37 0.39 0.48 – 0.57 0.56 0.26 0.2 0.3 0.11 0.11 0.12 0.13 0.05 0.06 0.07 0.11 0.22
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 1 0 1 0 – 0 0 0 1 2 0 0 0 1 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 2 6 2 – 3 8 0 1 6 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 1 2 1 3 11 3 11 7 2 – 4 3 8 23 29 12 9 23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 2 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 1 1 0 0 0 0 0 0 2
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 0 0 0 0 1
Mitochondria 6 1 3 1 2 11 17 25 26 – 19 17 2 1 11 5 11 36 0 0 0 0 0 3
Nucleus 232 178 114 184 365 243 385 472 251 – 115 256 224 375 420 175 141 236 214 175 106 131 240 288
Nuclear Periphery 0 0 0 0 0 0 0 2 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 102 57 60 36 62 94 173 233 151 – 95 180 63 82 152 23 18 34 32 9 6 10 33 115
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 1 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 – 0 0 0 6 8 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 3 0 0 1 0 4 1 0 – 0 1 2 2 5 3 5 4 3 4 5 6 30 102
Unique Cell Count 252 189 121 191 397 277 473 591 313 166 324 239 418 506 201 167 285 253 192 119 152 306 521
Labelled Cell Count 341 242 178 224 442 353 592 747 432 236 466 299 493 635 222 188 334 253 192 119 152 306 521


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.6 29.2 19.2 19.6 22.6 19.1 19.4 18.0 16.3 – 15.4 16.2 32.0 29.8 26.0 36.2 37.2 38.6 28.3 28.1 29.7
Std Deviation (1e-4) 9.3 8.3 5.4 6.2 8.4 6.8 6.4 7.0 5.4 – 5.4 6.0 10.4 11.2 10.0 16.5 20.7 17.2 9.0 7.5 8.4
Intensity Change (Log2) – – – 0.03 0.23 -0.01 0.01 -0.1 -0.24 – -0.32 -0.25 0.73 0.63 0.43 0.91 0.95 1.0 0.56 0.55 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 2.2 2.3 0 0 2.8
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.2 – 2.8 1.3 0 0 0 0 1.6 3.2
Nucleus 0.9 -0.8 -2.0 -3.4 -3.8 -3.6 – -5.2 -3.8 -0.2 -1.5 -3.1 -2.0 -2.6 -3.0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus -5.7 -7.7 -2.9 -2.6 -2.1 -0.3 – 1.3 1.1 -4.4 -6.6 -4.1 -7.6 -7.3 -8.2
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.6244 27.7235 25.3686 18.8449 23.2352 24.5535 28.8895 33.6569 31.6364 29.6054 23.6357 31.4879 24.7998 26.495 24.3368 22.2767 19.5577 22.5696
Actin 0.0719 0 0.0375 0.0461 0 0 0.0299 0 0.0016 0.0222 0.0095 0.0043 0.0017 0 0.0051 0.0037 0.0013 0.0028
Bud 0.0004 0 0.0002 0.0017 0.0001 0.0001 0.0004 0 0.0005 0.0006 0.0006 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0003 0 0.0003 0.0007 0.0001 0.0001 0.0002 0 0.0002 0.0001 0.0003 0.0003 0.0003 0 0.0015 0.0001 0.0001 0.0001
Bud Periphery 0.0006 0 0.0002 0.0033 0.0003 0.0001 0.0004 0 0.0008 0.0007 0.0008 0.0005 0.0001 0 0.0002 0.0003 0.0001 0.0004
Bud Site 0.0024 0 0.0009 0.0017 0.0001 0 0.0008 0 0.0009 0.0009 0.0017 0.0001 0.0033 0 0.0041 0.0007 0.0001 0.0001
Cell Periphery 0.0002 0 0.0001 0.0004 0.0004 0.0002 0.0001 0 0.0004 0.0001 0.0004 0.0002 0.0001 0 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.0048 0 0.0002 0.0024 0 0 0.0057 0 0.0011 0.0017 0.0218 0.0001 0.0003 0 0 0 0 0
Cytoplasmic Foci 0.0176 0 0.0006 0.0096 0 0 0.0077 0 0.0071 0.0038 0.0074 0.0025 0.0082 0 0.0039 0.0006 0.0005 0
Eisosomes 0.0005 0 0.0003 0.0003 0 0 0.0001 0 0.0007 0.0001 0.0002 0 0 0 0.0004 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.004 0 0.0005 0.0037 0 0 0.0015 0 0.0002 0.0007 0.0045 0.0003 0.0001 0 0 0 0 0.0002
Endosome 0.0228 0 0.0029 0.065 0 0 0.0098 0 0.0011 0.003 0.0254 0.0015 0.0031 0 0.0001 0.0003 0.0004 0.0005
Golgi 0.0108 0 0.0068 0.0545 0 0 0.002 0 0.0004 0.0012 0.0025 0.0013 0.0016 0 0.0002 0.0005 0.001 0.0005
Lipid Particles 0.0137 0 0.0003 0.0054 0 0 0.0023 0 0.0031 0.0014 0.0022 0.0128 0.0079 0 0.0008 0.0013 0.0065 0.0002
Mitochondria 0.0123 0.0008 0.003 0.1792 0.0021 0.0006 0.003 0.0013 0.0048 0.0107 0.1092 0.0017 0.0019 0.01 0.0037 0.0433 0.0238 0.0086
None 0.0067 0 0.0001 0.0006 0 0 0.0069 0 0.0017 0.0018 0.0063 0.0001 0.0005 0 0.0001 0.0001 0.0001 0.0001
Nuclear Periphery 0.0246 0.0007 0.001 0.0023 0 0.0001 0.0148 0.0002 0.0059 0.0154 0.041 0.0079 0.0004 0.0003 0.0001 0.0002 0.0002 0.0004
Nucleolus 0.0353 0.0285 0.0289 0.0071 0.4129 0.1345 0.0689 0.0228 0.0393 0.0353 0.1452 0.0948 0.0313 0.0189 0.024 0.018 0.2593 0.1027
Nucleus 0.751 0.969 0.9153 0.5938 0.5832 0.8639 0.8194 0.9752 0.9201 0.8748 0.589 0.8681 0.9235 0.9706 0.9544 0.9191 0.6912 0.8825
Peroxisomes 0.0041 0 0.0001 0.0024 0 0 0.0017 0 0.0004 0.0055 0.0017 0.0006 0.0073 0 0.0005 0.0111 0.0139 0.0001
Punctate Nuclear 0.0103 0 0.0001 0.0003 0 0 0.023 0 0.0062 0.0184 0.0229 0.0018 0.0075 0 0 0 0.0001 0
Vacuole 0.0024 0.0001 0.0002 0.0091 0.0003 0.0003 0.0007 0.0001 0.0014 0.0004 0.003 0.0004 0.0002 0 0.0002 0.0001 0.0004 0.0001
Vacuole Periphery 0.0033 0.0007 0.0005 0.0105 0.0004 0.0002 0.0009 0.0002 0.0019 0.0011 0.0046 0.0005 0.0002 0.0001 0.0004 0.0001 0.0006 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 147.1561 93.3059 74.1825 104.1228 121.289 113.1889 79.3399 76.0191 92.1376 136.6149
Translational Efficiency 1.6481 1.5668 1.5103 1.4322 1.56 1.8935 1.336 1.169 1.1371 1.2742

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; specifically required for diauxic shift-induced H2B deposition onto rDNA genes; mutations cause reduced nucleosome occupancy over highly transcribed regions; coregulates transcription with Mot1p through preinitiation complex assembly and nucleosome organization
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Spt16

Spt16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available