Standard name
Human Ortholog
Description Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.09 0.07 0.07 0.05 0.08 0 0.07 0.05 0.05 0.1 0.14 0.27 0.24 0.1 – 0 0 0 0.05 0.11 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.15 – 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.19 0.32 0.17 0.19 0.39 0.39 0.36 0 0 0 0 0 – 0 0 0 0 0 0
Nucleus 0.94 0.74 0.86 0.83 0.76 0.68 0.7 0.68 0.74 0.63 0.61 0.62 0.85 0.8 0.58 0.42 0.5 – 0.89 0.96 0.84 0.77 0.59 0.46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0.07 0.21 0 0 0 0.05 0 0.07 0.08 0.1 0.14 0.09 0 0 0 0 0 – 0 0 0.05 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07
Vac/Vac Membrane 0 0.32 0.08 0.05 0.13 0.15 0.07 0.1 0.07 0.09 0.1 0.07 0 0.1 0.19 0.31 0.4 – 0 0 0 0.06 0.14 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1
Bud 0 0 0 0 0 1 6 7 0 4 5 4 0 0 0 0 0 – 1 0 3 2 12 21
Bud Neck 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 3
Bud Site 0 0 0 0 0 3 7 13 5 13 10 14 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 2 0 12 19 24 30 27 43 15 38 21 25 20 60 116 17 2 – 1 0 2 8 36 51
Endoplasmic Reticulum 0 0 0 0 0 1 2 0 0 0 0 1 0 1 0 2 0 – 0 0 1 0 1 2
Endosome 0 0 3 2 13 8 5 17 4 4 2 3 2 3 17 9 3 – 0 0 2 1 4 20
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 11 0 3 4 15 78 169 98 78 220 168 170 0 0 0 3 0 – 0 0 2 1 5 5
Nucleus 127 14 217 176 257 276 372 383 309 358 265 292 174 349 253 30 10 – 126 48 228 119 189 193
Nuclear Periphery 0 0 1 0 0 1 1 5 1 0 0 5 0 0 0 0 0 – 0 0 0 0 0 2
Nucleolus 9 4 6 6 8 19 23 41 35 57 59 42 1 0 0 1 0 – 5 1 12 2 9 15
Peroxisomes 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 1 0 2 0 0 1 0 1 2 1 0 – 1 0 2 3 8 27
Vac/Vac Membrane 3 6 19 11 45 63 37 56 28 51 42 35 9 44 81 22 8 – 4 0 11 9 46 64
Unique Cell Count 135 19 251 211 340 407 531 564 415 564 431 470 204 438 433 72 20 142 51 271 156 323 422
Labelled Cell Count 152 24 261 218 363 481 652 665 477 745 572 592 206 458 469 85 23 142 51 271 156 323 422


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 7.7 7.9 7.2 6.5 5.8 5.8 5.8 6.1 5.7 5.7 5.5 9.3 9.0 7.9 10.5 11.3 – 7.7 8.1 8.1
Std Deviation (1e-4) 0.9 1.4 1.2 1.5 1.4 1.2 1.3 1.4 1.2 1.3 1.3 1.2 1.5 1.7 1.4 2.3 2.5 – 1.4 1.4 1.5
Intensity Change (Log2) – – – -0.12 -0.28 -0.45 -0.45 -0.44 -0.38 -0.46 -0.46 -0.5 0.25 0.19 0.02 0.42 0.52 – -0.03 0.04 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm 1.8 1.1 1.3 0.2 1.5 -0.7 1.1 0.1 0.3 2.1 3.7 7.1 4.9 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 1.9 0 0 1.5 0 0 0 0 0 0 2.0 0 0 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 2.2 6.8 9.7 6.5 6.7 11.2 11.0 10.5 0 0 0 0 0 –
Nucleus -0.9 -3.3 -5.4 -5.0 -5.5 -3.7 -6.6 -6.9 -6.8 -0.4 -2.2 -7.6 -7.9 -4.3 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0.3 0 1.5 1.3 2.8 3.1 3.8 4.8 3.4 0 0 0 0 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole -1.0 2.2 3.0 -0.3 1.1 -0.4 0.7 1.0 -0.1 -1.4 1.1 4.0 5.2 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2495 2.9385 2.339 2.135 1.7667 2.4954 3.5248 3.954 3.7425 3.5925 4.1562 3.8455 3.1036 3.5148 3.2292 3.0187 3.4323 3.3527
Actin 0.0168 0 0.0044 0.0264 0.067 0.0009 0.0401 0 0.0081 0.0234 0.0277 0.0009 0.0116 0 0.0045 0.0028 0.0045 0.0046
Bud 0.0003 0 0.0002 0.0002 0.0006 0.0001 0.0008 0 0.0003 0.0012 0.0005 0.0004 0.0002 0.0001 0.0001 0.0008 0.0002 0.0001
Bud Neck 0.0018 0.0001 0.007 0.0017 0.0014 0.0012 0.0105 0.0001 0.0049 0.001 0.0013 0.0017 0.0029 0.0002 0.0002 0.0009 0.0269 0.001
Bud Periphery 0.0009 0 0.0004 0.0004 0.001 0.0001 0.0015 0 0.0005 0.0036 0.0009 0.0008 0.0003 0 0.0001 0.0016 0.0003 0.0002
Bud Site 0.0052 0.0001 0.0034 0.0004 0.0011 0.0001 0.0028 0.0001 0.0011 0.0041 0.0018 0.0015 0.0008 0.0002 0.0002 0.0016 0.0012 0.0001
Cell Periphery 0.0006 0 0.0002 0.0001 0.0001 0 0.0004 0 0.0001 0.0006 0.0001 0.0001 0.0001 0 0 0.0004 0.0001 0
Cytoplasm 0.0081 0.0062 0.0037 0.0013 0.084 0.0015 0.0122 0.0009 0.0019 0.0011 0.0023 0.001 0.0081 0.006 0.0025 0.0082 0.0011 0.0004
Cytoplasmic Foci 0.006 0.0001 0.0012 0.0052 0.0056 0.0019 0.0122 0 0.002 0.0042 0.0021 0.0003 0.0156 0 0.0032 0.0176 0.0044 0.0008
Eisosomes 0.0002 0 0.0001 0.0002 0.0004 0 0.0003 0 0.0001 0.0004 0.0003 0 0.0001 0 0 0 0 0.0001
Endoplasmic Reticulum 0.003 0.0004 0.0006 0.0013 0.0028 0.0002 0.0076 0 0.0007 0.0018 0.002 0.0003 0.0029 0.0004 0.0008 0.0036 0.0003 0.0006
Endosome 0.0186 0.0003 0.0018 0.0071 0.0094 0.0042 0.0217 0 0.0014 0.0125 0.0138 0.0018 0.0317 0.0005 0.0064 0.0304 0.0066 0.002
Golgi 0.004 0 0.0005 0.003 0.004 0.0016 0.0044 0 0.001 0.004 0.0079 0.0003 0.003 0 0.0019 0.0072 0.0034 0.0008
Lipid Particles 0.0145 0 0.0008 0.0057 0.0041 0.0044 0.0082 0 0.0007 0.0193 0.0071 0.0015 0.0114 0 0.0068 0.0427 0.003 0.0025
Mitochondria 0.0112 0.0001 0.0006 0.0009 0.0011 0.0009 0.0044 0.0001 0.0026 0.0119 0.0032 0.0015 0.0024 0.0001 0.001 0.0087 0.0003 0.0044
None 0.0112 0.0005 0.0075 0.0028 0.0358 0.0002 0.0085 0 0.0039 0.001 0.0008 0.0002 0.0052 0.0001 0.0006 0.0012 0.0002 0.0003
Nuclear Periphery 0.0105 0.0083 0.0099 0.0275 0.0155 0.0048 0.0265 0.0015 0.0034 0.0186 0.0174 0.0068 0.0239 0.0062 0.0086 0.0117 0.0019 0.0062
Nucleolus 0.0074 0.0054 0.0059 0.0065 0.0141 0.0114 0.0088 0.0038 0.0037 0.0056 0.0079 0.007 0.0048 0.0033 0.0028 0.0078 0.0065 0.0062
Nucleus 0.8559 0.9696 0.9431 0.881 0.7375 0.9629 0.7987 0.9835 0.9585 0.8262 0.8917 0.9698 0.8471 0.9809 0.9558 0.7566 0.9347 0.9667
Peroxisomes 0.0027 0 0.0006 0.0022 0.0016 0.0011 0.007 0 0.0018 0.0194 0.0018 0.0004 0.0041 0 0.0015 0.0598 0.0009 0.0004
Punctate Nuclear 0.0165 0.0084 0.0053 0.0244 0.0112 0.002 0.0154 0.0098 0.0027 0.0354 0.0079 0.0026 0.0185 0.0013 0.0024 0.011 0.0027 0.0011
Vacuole 0.0026 0.0003 0.0019 0.0009 0.001 0.0003 0.0058 0.0001 0.0004 0.0025 0.0009 0.0007 0.003 0.0006 0.0003 0.0182 0.0007 0.0006
Vacuole Periphery 0.0019 0.0001 0.0009 0.0009 0.0006 0.0002 0.0023 0 0.0002 0.002 0.0008 0.0003 0.0022 0.0001 0.0003 0.0073 0.0001 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.5238 14.5939 21.6586 26.0985 8.7157 18.2359 24.5208 24.6051 25.2287 18.6862
Translational Efficiency 1.8818 2.2681 1.7585 0.9328 2.6683 1.8206 1.3692 1.6703 1.6997 1.5187

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Mig2

Mig2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mig2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available