Standard name
Human Ortholog
Description Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.66 0.39 0.36 0.49 0.37 0.54 0.46 0.43 0.38 0.38 0.44 0.41 0.26 0.39 0.45 0.55 0.5 0.45 0.38 0.07 0.05 0.09 0.06 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.19 0.35 0.53 0.46 0.42 0.26 0.29 0.25 0.26 0.35 0.15 0.2 0.46 0.43 0.41 0.33 0.37 0.42 0.27 0.59 0.62 0.55 0.64 0.59
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.18 0.25 0.3 0.34 0.23 0.38 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.08 0 0 0.05 0 0.06 0 0 0 0.13 0.13 0.09 0.05 0 0.06 0 0 0.05 0.06 0.06 0.09
Vac/Vac Membrane 0.14 0.46 0.31 0.18 0.23 0.09 0.09 0.11 0.16 0.16 0.12 0.13 0.46 0.33 0.25 0.3 0.33 0.38 0.23 0.24 0.22 0.24 0.17 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 11 2 0 0 2 1 1 1 0 1 4 0 3 5 7 4 3 1 9 2 1 1 0
Bud 3 5 13 1 2 15 13 23 12 25 18 20 1 2 3 0 1 0 1 8 3 5 3 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 1 1
Bud Site 0 0 0 0 0 1 0 1 2 3 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 1 1 0 1 0 3 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 82 259 124 78 74 212 202 208 151 221 208 226 94 200 230 224 115 108 54 50 17 29 19 36
Endoplasmic Reticulum 2 15 7 1 6 11 7 6 5 1 8 10 3 0 2 14 7 6 1 3 0 0 4 7
Endosome 24 236 181 72 84 101 129 124 106 207 72 111 164 220 205 133 85 101 38 432 224 186 230 289
Golgi 0 3 0 0 1 0 1 3 0 0 2 0 1 0 1 8 3 1 1 5 1 0 0 5
Mitochondria 3 3 4 4 19 69 111 144 138 132 180 187 0 0 0 10 5 1 2 5 2 5 5 2
Nucleus 0 1 0 0 1 2 1 2 0 0 0 1 0 1 0 0 0 1 0 5 0 2 2 1
Nuclear Periphery 1 1 1 0 0 0 0 0 0 1 2 4 0 0 0 0 0 1 1 7 6 2 4 8
Nucleolus 3 17 18 4 2 8 12 19 9 21 20 10 16 18 26 11 8 7 0 2 0 1 0 1
Peroxisomes 0 0 1 4 0 0 5 1 6 2 1 3 3 0 0 0 1 0 0 0 0 0 0 1
SpindlePole 5 25 21 13 3 10 22 20 23 24 8 12 46 69 47 19 9 14 3 23 18 21 21 44
Vac/Vac Membrane 17 305 107 28 47 37 39 56 65 94 59 71 163 171 129 121 75 91 32 173 77 79 60 79
Unique Cell Count 124 668 344 158 201 391 439 487 401 585 473 546 356 514 506 406 230 242 144 734 360 339 361 493
Labelled Cell Count 142 881 479 205 239 469 544 609 518 732 579 663 491 684 649 547 313 334 144 734 360 339 361 493


Cytoplasm, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 8.6 7.2 7.0 6.5 6.4 5.6 6.0 6.1 5.9 5.8 6.0 8.8 8.7 8.8 11.4 10.7 10.8 9.4 9.6 9.8
Std Deviation (1e-4) 2.4 1.1 1.0 1.2 1.2 1.0 0.9 1.0 1.3 1.1 1.1 1.1 1.1 1.1 1.0 2.0 2.1 1.7 1.3 1.4 1.5
Intensity Change (Log2) – – – -0.04 -0.15 -0.18 -0.37 -0.26 -0.25 -0.29 -0.31 -0.28 0.28 0.27 0.28 0.65 0.57 0.58 0.38 0.4 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -1.9 0 -0.6 0.7 -0.6 0.4 0 -0.1 -3.3 -3.7 -3.4 -4.0 -2.5 -3.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 0.2 4.9 2.8 1.9 0.5 0.5 2.3 1.6 -2.8 0.8 2.7 5.2 3.3 2.1
Endoplasmic Reticulum 0 0 0.7 -0.5 -0.9 -0.9 0 -0.4 -0.2 0 0 0 1.2 0.8 0.4
Endosome -1.5 -2.4 -7.5 -6.6 -8.0 -7.3 -5.1 -11.4 -10.0 -1.7 -2.8 -3.5 -5.5 -3.7 -2.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.6 7.5 9.5 10.5 11.5 8.9 12.5 11.7 0 0 0 1.3 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.4 -2.5 -2.3 -1.8 -0.9 -2.2 -1.2 -0.7 -2.8 -0.5 -1.2 -0.1 -1.8 -1.0 -1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.9 -2.5 -2.4 -0.7 -1.3 -0.2 -1.4 -3.4 -3.0 3.1 3.4 1.7 -0.9 -1.2 -0.2
Vacuole -3.1 -1.9 -7.4 -7.9 -7.0 -4.8 -5.4 -6.5 -6.6 4.0 0.7 -1.8 -0.4 0.4 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1686 4.0149 3.7482 3.7297 3.3287 3.6133 5.9904 6.756 6.8184 6.36 6.5941 6.7711 3.8426 5.0096 4.6721 4.7037 4.0827 4.3499
Actin 0.0038 0.0002 0.0002 0.0005 0.0002 0.0002 0.0048 0.0002 0.0002 0.0007 0 0.0004 0.0212 0.0001 0.0145 0.0073 0.0026 0.004
Bud 0.0072 0.0088 0.0049 0.0151 0.0007 0.0012 0.0003 0.001 0.0007 0.0037 0.0005 0.0004 0.0007 0.0004 0.0005 0.0054 0.0009 0.0005
Bud Neck 0.0022 0.0011 0.0015 0.0004 0.0012 0.0034 0.0006 0.0012 0.0009 0.0004 0.0007 0.0012 0.0031 0.001 0.0015 0.0013 0.0008 0.0014
Bud Periphery 0.0029 0.0011 0.0016 0.0054 0.0002 0.0005 0.0001 0.0004 0.0005 0.0014 0.0002 0.0003 0.0009 0.0002 0.0003 0.0038 0.0017 0.0004
Bud Site 0.0139 0.0065 0.005 0.0018 0.0008 0.0026 0.0009 0.0049 0.0014 0.0009 0.0026 0.0007 0.0049 0.0035 0.0038 0.0063 0.0023 0.0007
Cell Periphery 0.0003 0.0001 0.0002 0.0003 0 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0003 0.0002 0.0001 0.0002 0.0002 0.0001
Cytoplasm 0.2626 0.3255 0.3033 0.2027 0.1679 0.2782 0.1694 0.2967 0.2316 0.2492 0.1217 0.1616 0.1294 0.2854 0.2327 0.2282 0.1304 0.1986
Cytoplasmic Foci 0.1816 0.1855 0.2124 0.2217 0.2639 0.2281 0.1981 0.1999 0.2319 0.2809 0.2178 0.2729 0.1784 0.145 0.1571 0.1786 0.2067 0.1982
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0042 0.0035 0.0031 0.0016 0.0015 0.0028 0.0042 0.0036 0.003 0.0024 0.0049 0.0033 0.0041 0.0038 0.0032 0.006 0.0033 0.0041
Endosome 0.3589 0.3294 0.3417 0.3949 0.4253 0.3473 0.5103 0.375 0.439 0.4074 0.53 0.4395 0.4232 0.3526 0.4361 0.3663 0.4461 0.4133
Golgi 0.0072 0.008 0.0101 0.0072 0.0114 0.0076 0.0115 0.0106 0.0133 0.0151 0.0102 0.0149 0.0238 0.0096 0.0125 0.0064 0.0098 0.0107
Lipid Particles 0.01 0.0099 0.0058 0.0047 0.0069 0.0049 0.0082 0.0057 0.0101 0.0064 0.0027 0.0072 0.0551 0.0226 0.0118 0.0024 0.0097 0.0088
Mitochondria 0.0013 0.0014 0.0023 0.0021 0.001 0.0014 0.0008 0.0023 0.0011 0.0012 0.0031 0.0026 0.0104 0.0018 0.0012 0.0025 0.0103 0.0089
None 0.0467 0.0135 0.0184 0.0688 0.0093 0.0224 0.0073 0.0131 0.0106 0.0056 0.0012 0.017 0.0154 0.0091 0.0028 0.006 0.0022 0.0022
Nuclear Periphery 0.0057 0.0088 0.0076 0.0045 0.0043 0.0058 0.0039 0.0034 0.0054 0.0009 0.0025 0.0035 0.0048 0.0041 0.0035 0.0217 0.0347 0.0106
Nucleolus 0.0011 0.0035 0.0044 0.0021 0.0009 0.0017 0.0003 0.0011 0.0005 0.0001 0.0002 0.0005 0.0018 0.0052 0.0032 0.0007 0.0067 0.001
Nucleus 0.015 0.014 0.019 0.0076 0.0089 0.0151 0.0082 0.0074 0.0047 0.0011 0.0016 0.0059 0.0073 0.0097 0.0166 0.0119 0.017 0.0171
Peroxisomes 0.0104 0.0071 0.0095 0.007 0.0111 0.0075 0.0051 0.0053 0.0096 0.0074 0.0273 0.012 0.0146 0.0035 0.008 0.0023 0.0023 0.0037
Punctate Nuclear 0.0125 0.0072 0.0132 0.0093 0.0202 0.0138 0.0076 0.0107 0.004 0.001 0.0349 0.0082 0.0134 0.0132 0.0056 0.0179 0.0075 0.0057
Vacuole 0.0464 0.0584 0.0305 0.0366 0.0548 0.0458 0.052 0.05 0.026 0.0109 0.0296 0.0357 0.0783 0.1176 0.0755 0.108 0.0928 0.0923
Vacuole Periphery 0.0063 0.0064 0.0053 0.0058 0.0096 0.0095 0.0064 0.0073 0.0054 0.0033 0.008 0.0121 0.0085 0.0113 0.0093 0.017 0.012 0.0179

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.7019 6.2232 8.4661 8.4433 12.0346 12.7558 10.7493 7.0781 10.1604 9.4995
Translational Efficiency 2.8595 3.6219 1.9269 2.2133 2.138 1.8574 2.3377 3.405 1.9294 2.0252

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages vacuolar membrane (27%), endosome (7%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Vam7

Vam7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vam7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available