Standard name
Human Ortholog
Description Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.08 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0 0.05 0.06 0 0 0 0 0 0 0.08 0.06 0.09 0.11 0.09 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.12 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.08 0.09 0.05 0 0 0 0 0 0 0 0.14 0.17 0.21 0.17 0.2 0.22 0.11 0.11 0.08 0.08 0.07 0.05
Golgi 0.09 0.23 0.25 0.14 0.08 0 0 0 0 0 0 0 0.56 0.58 0.54 0.47 0.53 0.36 0.71 0.64 0.74 0.7 0.75 0.8
Mitochondria 0.96 0.86 0.85 0.89 0.89 0.95 0.96 0.95 0.94 0.96 0.95 0.97 0.29 0.15 0.17 0.12 0.11 0.1 0.09 0.15 0.08 0.14 0.08 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.06 0 0 0.06 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0.12 0.17 0.11 0.19 0.12 0.22 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 32 2 2 0 0 0 0 0 3 0 0 15 8 8 7 3 2 3 11 5 8 19 16
Bud 4 6 7 5 3 5 23 8 8 13 9 7 1 1 4 5 7 8 0 1 1 1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud Site 1 0 0 0 0 1 5 6 2 6 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 4 0 2 0 3 0 3 3 4 1 1 1 1 5 2 6 0 0 0 0 0 0
Cytoplasm 5 25 1 5 7 9 4 5 4 5 3 5 25 32 47 40 29 22 0 1 0 3 5 2
Endoplasmic Reticulum 10 47 5 2 1 0 0 0 4 2 2 0 16 11 12 9 11 9 0 2 0 1 6 6
Endosome 2 3 20 14 7 1 0 4 4 2 1 0 42 90 108 60 63 50 26 49 22 35 42 25
Golgi 20 92 61 22 12 0 0 1 2 0 1 0 171 299 283 168 166 84 166 296 196 317 443 422
Mitochondria 222 350 205 139 132 132 261 234 198 264 166 205 90 79 87 44 34 23 21 71 20 62 46 21
Nucleus 0 1 0 0 0 0 1 3 1 2 0 0 0 0 3 2 3 1 0 1 0 1 2 0
Nuclear Periphery 1 2 1 2 0 2 0 2 3 2 0 0 1 2 3 4 2 1 0 0 0 0 1 3
Nucleolus 2 1 1 3 0 2 5 11 3 3 4 2 1 0 0 2 0 1 0 0 0 0 0 0
Peroxisomes 0 0 8 8 1 4 7 6 4 8 7 13 1 1 1 6 7 15 7 2 14 4 9 15
SpindlePole 0 1 4 1 1 1 5 4 9 4 2 2 7 23 25 21 14 15 2 1 0 1 0 0
Vac/Vac Membrane 9 22 6 0 5 2 4 4 9 0 0 4 36 86 57 68 39 50 1 19 1 14 9 4
Unique Cell Count 231 407 242 156 149 139 273 247 210 275 174 212 308 516 523 358 315 232 234 461 264 456 591 525
Labelled Cell Count 281 583 325 203 171 159 318 288 254 317 201 240 407 633 639 441 381 287 234 461 264 456 591 525


Early Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 7.1 7.0 6.4 6.2 5.6 5.3 5.1 5.1 4.7 4.4 4.7 8.7 8.4 8.3 9.2 8.5 8.1 9.7 9.8 10.2
Std Deviation (1e-4) 0.8 1.0 0.9 0.9 1.4 2.1 1.4 1.1 1.3 1.0 1.1 0.8 1.0 1.0 1.3 1.6 1.6 1.8 1.3 1.3 1.7
Intensity Change (Log2) – – – -0.12 -0.16 -0.31 -0.39 -0.46 -0.46 -0.57 -0.66 -0.57 0.32 0.27 0.25 0.4 0.29 0.22 0.47 0.49 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 0 0 0 2.7 0.2 0.5 – – – 0 0 0 0 -0.5 0.3
Bud Neck 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 0 0 0 – – – 1.9 0.1 0.2 0.4 1.0 1.2
Endosome 0.2 -1.4 -3.1 -4.8 -3.4 -3.0 – – – 2.0 3.3 4.3 3.0 3.9 4.1
Golgi -2.7 -4.2 -6.5 -8.8 -8.2 -7.4 – – – 7.1 8.4 7.5 5.4 6.5 2.6
Mitochondria 1.2 1.1 3.0 4.2 3.7 3.3 – – – -13.0 -18.4 -18.0 -17.7 -17.5 -16.3
Nuclear Periphery 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 0 0 2.9 0 – – – 0 0 0 0 0 0
Peroxisomes 0.9 0 0 -0.5 -0.6 -0.9 – – – 0 0 0 -1.3 -0.8 1.6
SpindlePole 0 0 0 0 0 1.7 – – – 0 1.9 2.1 2.5 1.8 2.7
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 0 0 0 – – – 2.7 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – – – 4.2 3.6 4.5 5.1 4.6 4.6
Nucleus 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vacuole 0 0 0 0 0 1.1 – – – 4.0 5.6 3.9 6.0 4.3 6.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0244 0.019 0.0126 0.0013 0 0.0124 0.0437 0.0116 0.0095 0.0155 0.0034 0.0098 0.0164 0.0192 0.0238 0.0034 0.0454 0.0092
Bud 0.0005 0.0005 0.0002 0.0002 0 0.0001 0.0011 0.001 0.0011 0.0008 0.0003 0.0017 0.0018 0.0036 0.0176 0.025 0.0004 0.0028
Bud Neck 0.0008 0.0009 0.0016 0.0002 0.0001 0.0006 0.0038 0.0005 0.0012 0.001 0.0005 0.0005 0.0006 0.0005 0.0005 0.0005 0.0006 0.0015
Bud Periphery 0.0005 0.0009 0.0002 0.0003 0 0.0002 0.0011 0.0008 0.0008 0.0008 0.0003 0.0019 0.0013 0.0016 0.0085 0.0036 0.0004 0.0014
Bud Site 0.0011 0.0038 0.001 0.0002 0 0.0002 0.019 0.006 0.0123 0.003 0.001 0.0004 0.0099 0.0172 0.0221 0.0013 0.0007 0.0045
Cell Periphery 0.0002 0.0019 0.0002 0.0001 0 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002 0.0006 0.0003 0.0001 0.0002
Cytoplasm 0.0257 0.0082 0.0289 0.003 0.0004 0.0013 0.022 0.0055 0.0049 0.004 0.004 0.0129 0.0049 0.0132 0.0076 0.0156 0.0053 0.0106
Cytoplasmic Foci 0.0849 0.1082 0.0822 0.0504 0.0821 0.0627 0.0792 0.1129 0.0905 0.0753 0.1165 0.0705 0.1104 0.1352 0.1127 0.185 0.1189 0.106
Eisosomes 0.0002 0.0003 0.0001 0.0001 0 0.0001 0.0004 0.0001 0 0.0001 0 0.0001 0.0001 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0132 0.0016 0.0037 0.0008 0.0003 0.0026 0.0057 0.0016 0.0011 0.0026 0.0018 0.0015 0.0022 0.0009 0.0008 0.0007 0.0008 0.0013
Endosome 0.3209 0.2916 0.2453 0.1659 0.6343 0.161 0.2933 0.3179 0.3192 0.415 0.3907 0.1988 0.2733 0.3296 0.2214 0.2918 0.2624 0.1839
Golgi 0.4104 0.4841 0.5533 0.5313 0.2164 0.584 0.3597 0.4668 0.4919 0.4236 0.4259 0.6201 0.4914 0.4286 0.5323 0.4209 0.5083 0.6141
Lipid Particles 0.0157 0.0243 0.0172 0.002 0.0535 0.024 0.0447 0.0191 0.0105 0.0165 0.0129 0.0175 0.0237 0.0149 0.0034 0.01 0.0087 0.0163
Mitochondria 0.0459 0.0298 0.0208 0.2377 0.0017 0.1059 0.045 0.0403 0.032 0.0162 0.0199 0.0539 0.0307 0.0139 0.0276 0.0064 0.0368 0.0308
None 0.0073 0.002 0.0008 0.0001 0 0.0004 0.0084 0.0001 0.0002 0.0006 0.0004 0.0007 0.001 0.0013 0.0025 0.0002 0.0003 0.0036
Nuclear Periphery 0.0212 0.0004 0.0068 0.0001 0.0001 0.0223 0.0183 0.0004 0.0003 0.0049 0.002 0.0005 0.0073 0.0004 0.0003 0.0004 0.0006 0.0013
Nucleolus 0.0006 0.0002 0.0001 0 0 0.0002 0.0013 0.0001 0.0003 0.0005 0.0012 0.0001 0.0008 0.0004 0.0008 0.0002 0.0001 0.0001
Nucleus 0.0071 0.0002 0.0042 0.0001 0 0.0027 0.017 0.0001 0.0004 0.0017 0.0008 0.0001 0.0048 0.0007 0.0008 0.0042 0.0001 0.0005
Peroxisomes 0.0056 0.0166 0.0175 0.0009 0.0045 0.0132 0.0093 0.0098 0.0155 0.0065 0.0101 0.0045 0.0131 0.0096 0.0119 0.0213 0.0061 0.0074
Punctate Nuclear 0.0058 0.0003 0.0009 0 0.0001 0.0006 0.0101 0.0002 0.0021 0.0027 0.0007 0.0002 0.0007 0.0022 0.0012 0.0025 0.0005 0.0008
Vacuole 0.0035 0.003 0.001 0.002 0.0037 0.0019 0.0068 0.0026 0.003 0.0042 0.0045 0.0016 0.0025 0.0041 0.0019 0.0037 0.0016 0.0017
Vacuole Periphery 0.0044 0.0022 0.0014 0.0032 0.0025 0.0038 0.0096 0.0024 0.003 0.0045 0.0029 0.0026 0.0029 0.0025 0.0016 0.003 0.0017 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.5982 12.5853 10.5062 19.8816 12.5492 6.1559 13.5203 15.8351 15.8311 18.9928
Translational Efficiency 0.7615 1.0233 1.056 0.6112 1.0046 2.2843 1.0897 0.9326 0.9128 0.9552

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Cog1

Cog1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cog1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available