Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud 0 0 0.06 0.09 0 0 0.06 0.06 0 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.12 0 0 0 0 0 0
Cytoplasm 0.13 0.11 0.33 0.16 0.12 0.1 0.11 0.13 0.09 0.1 0.13 0.13 0.11 0.25 0.28 0.24 0.29 0.31 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0 0
Endosome 0 0.09 0 0 0 0 0 0 0 0 0 0 0.17 0.16 0.18 0.19 0.21 0.13 0.05 0.11 0.06 0.07 0.08 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Mitochondria 0.87 0.68 0.64 0.91 0.85 0.93 0.91 0.87 0.94 0.93 0.92 0.92 0.51 0.43 0.34 0.05 0.09 0.09 0.72 0.71 0.72 0.7 0.67 0.64
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.08 0.11 0.1 0.08 0 0 0 0 0.05 0
SpindlePole 0 0 0.07 0 0 0 0 0.05 0 0 0 0.06 0.05 0 0 0.17 0.16 0.26 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.11 0 0 0.07 0 0 0.05 0 0 0 0 0.17 0.12 0.15 0.25 0.2 0.15 0.1 0.05 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 5 0 0 1 0 1 0 0 1 0 0 4 7 1 0 0 0 1 4 7 6 24 37
Bud 0 3 21 21 8 12 29 26 17 35 16 29 5 14 5 0 2 0 0 2 2 3 5 5
Bud Neck 0 0 5 0 1 0 2 0 0 0 0 0 0 1 0 0 1 1 0 1 2 3 7 14
Bud Site 0 0 3 2 1 4 7 7 9 10 3 10 2 0 0 0 0 0 – – – – – –
Cell Periphery 3 10 4 3 3 2 2 0 3 3 0 1 22 15 14 14 14 18 0 0 1 0 2 0
Cytoplasm 13 23 115 40 36 27 52 57 41 49 45 58 57 113 144 40 47 47 4 1 10 3 3 1
Endoplasmic Reticulum 7 13 3 1 3 1 1 0 2 6 2 1 18 12 10 17 10 6 0 2 12 1 7 5
Endosome 0 18 3 0 7 1 0 0 0 0 0 0 88 71 95 31 34 20 5 30 23 28 55 53
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 14 11 2 1 1 4 0 7 3 21 36 25
Mitochondria 87 141 221 224 267 257 440 375 421 462 326 421 262 197 174 9 15 14 80 193 275 287 452 377
Nucleus 2 2 3 0 1 9 8 10 6 4 2 1 5 3 3 7 9 8 0 0 0 1 1 1
Nuclear Periphery 0 2 0 0 1 0 8 2 6 2 0 3 2 0 1 0 0 0 1 1 3 2 5 3
Nucleolus 0 0 0 0 0 3 3 3 5 1 1 2 1 1 1 0 1 1 1 0 0 1 2 2
Peroxisomes 0 3 3 3 6 0 3 1 1 1 1 2 34 23 43 18 16 12 0 5 12 16 32 20
SpindlePole 0 6 23 9 9 3 21 22 10 13 10 27 26 13 23 29 26 39 0 3 4 6 5 7
Vac/Vac Membrane 9 23 9 7 21 12 13 20 12 12 3 8 87 57 79 41 33 22 11 12 14 19 29 26
Unique Cell Count 100 208 344 247 313 276 483 432 448 499 354 458 515 457 516 167 163 151 113 275 384 411 677 588
Labelled Cell Count 124 250 413 310 365 331 590 523 533 599 409 563 627 538 595 207 209 192 113 275 384 411 677 588


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 5.3 3.3 3.2 3.9 3.3 3.2 3.3 2.8 3.0 3.0 2.9 5.5 5.4 5.3 10.8 7.1 7.4 5.6 5.4 5.5
Std Deviation (1e-4) 0.5 0.9 0.8 1.1 1.3 1.4 1.4 1.3 0.8 1.0 1.2 0.8 1.1 1.1 1.1 9.3 2.0 2.6 1.7 1.3 1.5
Intensity Change (Log2) – – – -0.04 0.24 -0.03 -0.07 0.01 -0.27 -0.17 -0.14 -0.21 0.72 0.71 0.67 1.7 1.1 1.15 0.76 0.71 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -4.3 -2.1 -4.3 -3.3 -2.5 -3.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 2.6 2.0 1.6 4.2 4.2 5.3
Cytoplasm -4.7 -6.7 -7.0 -8.0 -6.7 -8.5 -8.5 -6.5 -7.1 -8.0 -2.7 -1.7 -2.2 -1.0 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 2.4 1.8 1.3 5.1 0 0
Endosome 0 0 0 0 0 0 0 0 0 7.6 7.1 7.9 7.5 8.1 6.0
Golgi 0 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -3.9 -5.9 -8.8 -12.5 -11.6 -11.3
Nucleus 0 0 2.1 0 1.6 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 4.1 3.3 4.8 5.3 5.0 0
SpindlePole 0 0 0 0 0 0 0 0 0 -1.0 -2.6 -1.4 3.7 3.3 5.9
Vacuole 0.2 2.5 1.2 0.1 1.5 0.1 -0.2 -1.8 -0.8 6.5 5.0 6.0 7.8 6.7 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0836 1.7775 1.4008 1.1756 1.6672 1.4527 0.4828 0.5769 0.621 1.3328 0.6436 0.7128 1.8838 2.0028 1.9538 2.1469 1.0469 2.2768
Actin 0.0127 0.0147 0.007 0.0021 0.0143 0.0131 0.0192 0.0077 0.0193 0.0503 0.0082 0.0138 0.0592 0.0182 0.0113 0.0637 0.1473 0.0291
Bud 0.0008 0.0049 0.0036 0.0032 0.0097 0.0011 0.0032 0.0048 0.0041 0.0015 0.0212 0.0011 0.0018 0.0031 0.0085 0.0022 0.0058 0.0018
Bud Neck 0.0076 0.0023 0.0087 0.0127 0.0013 0.0057 0.002 0.0015 0.008 0.0015 0.0023 0.005 0.0049 0.0018 0.0016 0.0009 0.003 0.0088
Bud Periphery 0.0018 0.0075 0.0138 0.015 0.0239 0.0029 0.0065 0.0108 0.0099 0.004 0.0265 0.0042 0.0048 0.0062 0.008 0.0057 0.0168 0.0053
Bud Site 0.0065 0.0236 0.0076 0.0038 0.0045 0.0021 0.0108 0.0329 0.031 0.0029 0.006 0.0014 0.0049 0.033 0.0159 0.0021 0.0078 0.002
Cell Periphery 0.0014 0.0018 0.0029 0.0044 0.0005 0.0008 0.0019 0.0025 0.0022 0.0006 0.001 0.0008 0.0023 0.0019 0.0018 0.0008 0.0025 0.0016
Cytoplasm 0.0128 0.0413 0.0646 0.036 0.0272 0.0289 0.0231 0.0578 0.0364 0.0055 0.0184 0.0236 0.023 0.0377 0.0412 0.0118 0.0198 0.0521
Cytoplasmic Foci 0.0744 0.0984 0.0617 0.0245 0.0624 0.0691 0.0738 0.0965 0.0773 0.0829 0.0851 0.0586 0.0866 0.0943 0.1049 0.0316 0.0543 0.1026
Eisosomes 0.0004 0.0008 0.0005 0.0002 0.0003 0.0003 0.0011 0.0005 0.0009 0.0003 0.0002 0.0004 0.0025 0.0012 0.0018 0.0007 0.0135 0.0009
Endoplasmic Reticulum 0.0032 0.0054 0.0037 0.0026 0.0017 0.0034 0.005 0.0049 0.0025 0.0009 0.0033 0.0019 0.0171 0.0057 0.0023 0.0035 0.0023 0.0052
Endosome 0.1828 0.1573 0.16 0.1668 0.1746 0.1427 0.1607 0.1315 0.123 0.1089 0.1195 0.0935 0.159 0.1784 0.1291 0.0521 0.0962 0.1489
Golgi 0.075 0.0919 0.0776 0.0627 0.0576 0.0774 0.0534 0.0625 0.054 0.0902 0.0612 0.0531 0.0816 0.0908 0.0723 0.0278 0.0821 0.0963
Lipid Particles 0.034 0.0334 0.0282 0.0044 0.0535 0.0363 0.0426 0.0141 0.0172 0.0388 0.0312 0.0205 0.0403 0.0324 0.023 0.0109 0.0095 0.0469
Mitochondria 0.48 0.3869 0.4944 0.5727 0.5021 0.5346 0.4403 0.4723 0.4986 0.5492 0.4985 0.6119 0.3736 0.3877 0.4723 0.7043 0.4821 0.3904
None 0.0361 0.0518 0.0173 0.0082 0.004 0.0236 0.0652 0.0275 0.044 0.0101 0.0282 0.0293 0.0669 0.0485 0.0273 0.0379 0.0293 0.041
Nuclear Periphery 0.0024 0.0017 0.0026 0.0012 0.0094 0.0023 0.0088 0.0027 0.0012 0.0005 0.0105 0.005 0.0093 0.0013 0.0005 0.0016 0.0003 0.0018
Nucleolus 0.0013 0.0018 0.0008 0.0005 0.002 0.001 0.004 0.0014 0.0016 0.0012 0.0023 0.0006 0.0007 0.0011 0.0005 0.0005 0.0004 0.0005
Nucleus 0.0023 0.0019 0.0014 0.0013 0.0034 0.0019 0.0063 0.0031 0.0018 0.0008 0.0059 0.0013 0.0032 0.0015 0.0007 0.0008 0.0004 0.0008
Peroxisomes 0.024 0.036 0.0207 0.0083 0.019 0.0334 0.0394 0.0435 0.0458 0.0375 0.0455 0.0634 0.0383 0.0334 0.0616 0.0274 0.0192 0.0429
Punctate Nuclear 0.002 0.004 0.0008 0.0002 0.0126 0.0039 0.0063 0.0011 0.0007 0.0024 0.0073 0.0009 0.001 0.0054 0.0005 0.0004 0.0005 0.0007
Vacuole 0.0263 0.0225 0.0137 0.0392 0.0088 0.0093 0.0188 0.0154 0.014 0.006 0.0109 0.006 0.0142 0.0113 0.01 0.0081 0.0039 0.0142
Vacuole Periphery 0.012 0.0101 0.0084 0.0302 0.0072 0.006 0.0075 0.0051 0.0065 0.0042 0.0067 0.0037 0.0049 0.005 0.0051 0.005 0.003 0.0061

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.2122 10.6062 9.8378 14.923 24.9973 11.858 14.7454 16.2853 22.6688 17.5041
Translational Efficiency 1.6937 3.2058 2.6192 2.1067 1.4611 4.9863 2.3244 1.5751 1.7841 2.0181

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
Localization
Cell Percentages mitochondrion (79%), cytoplasm (9%), mixed (2%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mtc3

Mtc3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available