Standard name | |
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Human Ortholog | |
Description | Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 4 | 8 | 0 | 4 | 5 | 11 | 4 | 13 | 6 | 6 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 3 | 1 | 1 | 7 | 5 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 11 | 1 | 6 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 12 | 14 | 60 | 74 | 86 | 102 | 108 | 131 | 83 | 87 | 69 | 96 | 7 | 48 | 60 | 120 | 92 | 100 | 15 | 29 | 74 | 9 | 26 | 18 |
Endoplasmic Reticulum | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 9 | 14 | 11 | 0 | 0 | 2 | 1 | 0 | 2 |
Endosome | 5 | 0 | 0 | 1 | 8 | 2 | 5 | 3 | 0 | 0 | 1 | 0 | 0 | 7 | 4 | 26 | 30 | 26 | 3 | 1 | 7 | 1 | 5 | 2 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 21 | 99 | 145 | 103 | 174 | 274 | 352 | 181 | 234 | 198 | 284 | 7 | 10 | 26 | 4 | 1 | 7 | 1 | 3 | 25 | 0 | 5 | 2 |
Nucleus | 16 | 4 | 25 | 29 | 52 | 42 | 98 | 102 | 50 | 53 | 37 | 44 | 1 | 25 | 21 | 37 | 33 | 44 | 33 | 30 | 82 | 8 | 26 | 18 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 2 | 0 | 2 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 4 | 11 | 1 | 4 | 4 |
Vac/Vac Membrane | 25 | 14 | 43 | 30 | 61 | 61 | 72 | 95 | 30 | 48 | 29 | 59 | 9 | 66 | 75 | 179 | 155 | 195 | 21 | 18 | 89 | 5 | 28 | 28 |
Unique Cell Count | 47 | 45 | 208 | 228 | 269 | 314 | 441 | 543 | 266 | 333 | 266 | 375 | 20 | 139 | 166 | 323 | 265 | 322 | 89 | 99 | 312 | 30 | 109 | 92 |
Labelled Cell Count | 58 | 54 | 235 | 290 | 316 | 385 | 572 | 714 | 353 | 443 | 347 | 499 | 24 | 156 | 189 | 376 | 327 | 387 | 89 | 99 | 312 | 30 | 109 | 92 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 3.8 | 3.7 | 3.5 | 4.2 | 3.9 | 3.7 | 3.8 | 3.5 | 3.6 | 3.6 | 3.3 | 4.6 | 5.4 | 4.8 | 7.4 | 7.7 | 8.0 | – | 4.8 | 6.4 |
Std Deviation (1e-4) | 1.0 | 0.6 | 0.6 | 0.8 | 1.3 | 1.0 | 1.0 | 1.0 | 1.1 | 1.4 | 1.2 | 1.0 | 0.8 | 1.6 | 1.2 | 1.1 | 1.4 | 1.6 | – | 0.8 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.17 | 0.05 | 0.0 | 0.02 | -0.09 | -0.05 | -0.06 | -0.18 | 0.32 | 0.54 | 0.36 | 0.98 | 1.04 | 1.1 | – | 0.38 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 7.9 | 8.3 | 9.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0573 | 2.2562 | 2.2106 | 1.8922 | 1.9444 | 1.9046 | 1.5016 | 2.6117 | 2.2397 | 1.9465 | 2.1548 | 1.9797 | 3.5829 | 3.5078 | 2.884 | 3.5849 | 2.8282 | 3.2776 |
Actin | 0.0527 | 0.0004 | 0.0122 | 0.0252 | 0.0389 | 0.0003 | 0.0306 | 0.0005 | 0.0134 | 0.0006 | 0.0233 | 0.0006 | 0.0391 | 0.0003 | 0.0025 | 0.0002 | 0.0001 | 0.0094 |
Bud | 0.0015 | 0.0003 | 0.0004 | 0.0005 | 0.0019 | 0.0002 | 0.001 | 0.0003 | 0.0009 | 0.0001 | 0.0006 | 0.0002 | 0.0062 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0005 |
Bud Neck | 0.0055 | 0.0011 | 0.0096 | 0.0047 | 0.002 | 0.0044 | 0.0058 | 0.0008 | 0.0014 | 0.0011 | 0.0021 | 0.0041 | 0.0121 | 0.0003 | 0.0007 | 0.0006 | 0.0006 | 0.0049 |
Bud Periphery | 0.0029 | 0.0001 | 0.0003 | 0.0007 | 0.0032 | 0.0005 | 0.0013 | 0.0001 | 0.0009 | 0 | 0.0007 | 0.0001 | 0.0023 | 0 | 0.0002 | 0.0001 | 0 | 0.0005 |
Bud Site | 0.0102 | 0.0021 | 0.0037 | 0.0022 | 0.0063 | 0.0002 | 0.0136 | 0.0024 | 0.0065 | 0.0002 | 0.0046 | 0.0003 | 0.024 | 0.0006 | 0.0011 | 0.0001 | 0.0001 | 0.0009 |
Cell Periphery | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0007 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.1579 | 0.2869 | 0.201 | 0.1447 | 0.2892 | 0.1538 | 0.1839 | 0.2612 | 0.178 | 0.1505 | 0.1489 | 0.1476 | 0.1605 | 0.3454 | 0.2545 | 0.0909 | 0.3131 | 0.2132 |
Cytoplasmic Foci | 0.0288 | 0.0048 | 0.0127 | 0.0174 | 0.0066 | 0.0027 | 0.0198 | 0.0049 | 0.0166 | 0.0022 | 0.0164 | 0.0032 | 0.0231 | 0.0012 | 0.007 | 0.0004 | 0.0007 | 0.014 |
Eisosomes | 0.0011 | 0 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0008 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0178 | 0.0086 | 0.0108 | 0.0101 | 0.0128 | 0.0061 | 0.0087 | 0.0032 | 0.0033 | 0.0052 | 0.0032 | 0.0022 | 0.0062 | 0.0047 | 0.0078 | 0.0009 | 0.0028 | 0.0049 |
Endosome | 0.0428 | 0.0054 | 0.0191 | 0.018 | 0.027 | 0.0037 | 0.0305 | 0.003 | 0.0124 | 0.003 | 0.0203 | 0.0061 | 0.0201 | 0.0024 | 0.0115 | 0.0005 | 0.0012 | 0.0119 |
Golgi | 0.0121 | 0.0002 | 0.0031 | 0.0146 | 0.0107 | 0.0002 | 0.0073 | 0.0001 | 0.0044 | 0.0001 | 0.006 | 0.0016 | 0.0093 | 0 | 0.0056 | 0 | 0 | 0.0019 |
Lipid Particles | 0.015 | 0.0003 | 0.0094 | 0.0091 | 0.0054 | 0.0003 | 0.0109 | 0.0002 | 0.0033 | 0.0002 | 0.0117 | 0.0011 | 0.0081 | 0 | 0.001 | 0 | 0 | 0.0041 |
Mitochondria | 0.0167 | 0.0159 | 0.0025 | 0.0042 | 0.0434 | 0.0081 | 0.0163 | 0.001 | 0.0057 | 0.0011 | 0.0056 | 0.0006 | 0.0037 | 0.0004 | 0.002 | 0.0003 | 0.0004 | 0.0027 |
None | 0.0614 | 0.0348 | 0.0334 | 0.0127 | 0.0642 | 0.0089 | 0.1114 | 0.1086 | 0.1097 | 0.1372 | 0.092 | 0.0536 | 0.0473 | 0.02 | 0.0912 | 0.0873 | 0.0563 | 0.0643 |
Nuclear Periphery | 0.0589 | 0.0234 | 0.0355 | 0.0218 | 0.0333 | 0.0198 | 0.0272 | 0.0188 | 0.0153 | 0.042 | 0.0322 | 0.0107 | 0.0433 | 0.0281 | 0.0354 | 0.0073 | 0.0112 | 0.0209 |
Nucleolus | 0.0066 | 0.0031 | 0.0041 | 0.0022 | 0.0017 | 0.0042 | 0.0035 | 0.0023 | 0.0025 | 0.0019 | 0.0091 | 0.0038 | 0.003 | 0.0011 | 0.0021 | 0.001 | 0.0016 | 0.0029 |
Nucleus | 0.454 | 0.577 | 0.6021 | 0.6701 | 0.4295 | 0.7613 | 0.4738 | 0.5748 | 0.5894 | 0.6163 | 0.5481 | 0.7353 | 0.5315 | 0.5861 | 0.5545 | 0.8053 | 0.6041 | 0.5863 |
Peroxisomes | 0.0137 | 0.0003 | 0.008 | 0.0203 | 0.0012 | 0 | 0.0097 | 0.0001 | 0.0051 | 0.0001 | 0.0259 | 0.0003 | 0.0071 | 0 | 0.0005 | 0 | 0 | 0.0035 |
Punctate Nuclear | 0.0196 | 0.0276 | 0.0242 | 0.0177 | 0.0118 | 0.0202 | 0.0383 | 0.0145 | 0.0278 | 0.0362 | 0.0453 | 0.0262 | 0.047 | 0.0068 | 0.0197 | 0.0044 | 0.0064 | 0.0494 |
Vacuole | 0.0145 | 0.007 | 0.0061 | 0.0026 | 0.0078 | 0.0035 | 0.0044 | 0.0026 | 0.0024 | 0.001 | 0.0021 | 0.0021 | 0.0035 | 0.0019 | 0.0017 | 0.0004 | 0.0009 | 0.0029 |
Vacuole Periphery | 0.0055 | 0.0007 | 0.0012 | 0.0008 | 0.0023 | 0.0006 | 0.0012 | 0.0004 | 0.0007 | 0.0007 | 0.0008 | 0.0003 | 0.0017 | 0.0005 | 0.0007 | 0.0001 | 0.0002 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.8623 | 5.1427 | 6.1058 | 11.5257 | 7.4589 | 11.1161 | 10.2263 | 14.1551 | 16.8539 | 12.4176 |
Translational Efficiency | 0.7227 | 0.8178 | 0.8602 | 0.9001 | 0.793 | 0.9213 | 0.7072 | 0.564 | 0.6773 | 0.5858 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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