Standard name
Human Ortholog
Description Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.47 0.41 0.3 0.34 0.14 0.16 – 0.19 0.19 0.23 0.21 0.2 0.42 0.43 0.35 0.2 0.19 0.28 0.31 0.21 0.38 0.13 0.14 0.2
Bud 0.22 0.22 0.19 0.22 0.21 0.18 – 0.18 0.2 0.18 0.17 0.17 0.23 0.14 0.15 0.18 0.15 0.16 0.37 0.37 0.32 0.4 0.44 0.41
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0.2 0.25 0.13 0.3 0.26 0.15
Bud Site 0.27 0.33 0.11 0.09 0.08 0.11 – 0 0.06 0.08 0.08 0.06 0.07 0.08 0 0.13 0.11 0.12 – – – – – –
Cell Periphery 0.09 0 0.08 0.14 0 0.07 – 0.09 0.13 0.13 0.12 0.13 0.08 0.08 0.09 0.08 0.05 0 0 0 0 0 0 0
Cytoplasm 0.21 0.22 0.27 0.24 0.32 0.29 – 0.24 0.19 0.18 0.2 0.18 0.42 0.41 0.54 0.41 0.39 0.33 0 0.05 0.05 0.06 0.07 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.08 0.05 0.12 0.08 – 0.1 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.2 0 0.05 0 0.15 – 0.13 0.35 0.33 0.51 0.35 0 0 0 0.06 0.11 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.13 0.09 0.28 0.26 0.21 0.17 – 0.14 0.14 0.18 0.1 0.15 0 0 0 0.16 0.16 0.11 0 0 0 0 0 0
SpindlePole 0.21 0.07 0.14 0.11 0.19 0.07 – 0.16 0.1 0.06 0.06 0.08 0.13 0.17 0.11 0.15 0.23 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 88 93 99 132 77 80 – 151 87 151 98 124 44 108 81 48 130 82 62 52 144 27 51 98
Bud 42 49 64 87 117 88 – 145 94 123 79 107 24 35 34 44 99 48 75 93 123 85 164 201
Bud Neck 1 2 2 6 11 14 – 13 7 11 5 14 1 1 0 3 43 0 40 64 50 63 95 73
Bud Site 50 74 38 37 46 52 – 36 29 56 39 39 7 20 7 31 74 35 – – – – – –
Cell Periphery 16 9 27 56 24 32 – 71 61 87 57 84 9 19 21 20 33 12 4 1 9 0 3 6
Cytoplasm 39 49 90 96 183 143 – 196 91 123 95 115 44 102 123 97 265 97 7 13 19 12 25 60
Endoplasmic Reticulum 4 2 3 11 11 18 – 28 16 23 20 32 2 9 6 10 27 9 0 1 5 1 3 9
Endosome 2 5 26 21 68 41 – 84 23 40 4 18 1 3 0 7 26 11 1 4 8 4 4 8
Golgi 0 1 0 2 1 0 – 0 0 0 0 0 0 0 0 1 2 1 0 0 2 1 1 1
Mitochondria 11 46 8 21 13 72 – 102 164 223 241 221 0 0 0 14 74 17 5 8 7 6 5 13
Nucleus 4 5 4 3 6 5 – 20 5 5 7 7 1 4 2 7 15 14 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 – 2 1 1 0 1 0 0 0 0 0 0 0 1 0 0 1 1
Nucleolus 1 5 5 8 22 17 – 33 14 17 10 24 0 0 0 2 5 2 0 0 0 0 0 0
Peroxisomes 25 20 92 101 120 85 – 112 66 122 45 92 1 1 3 37 106 31 0 1 3 1 0 3
SpindlePole 39 16 46 45 110 36 – 130 48 43 30 52 14 42 25 35 158 52 2 5 5 3 3 7
Vac/Vac Membrane 1 3 2 2 12 9 – 37 14 18 7 21 1 1 0 3 12 7 0 3 1 1 4 6
Unique Cell Count 187 225 332 393 569 486 805 467 671 472 634 106 251 229 238 681 293 203 253 385 212 371 496
Labelled Cell Count 323 379 506 628 821 693 1160 720 1043 737 951 149 345 302 359 1069 418 203 253 385 212 371 496


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 6.0 7.5 7.5 7.4 6.5 – 6.6 6.2 5.9 5.2 6.0 7.5 8.1 8.2 5.6 6.6 7.9 8.6 8.7 8.7
Std Deviation (1e-4) 1.1 1.3 1.5 1.6 1.7 1.5 – 1.5 1.5 1.4 1.3 1.3 0.9 1.0 1.3 1.0 1.3 1.6 1.3 1.7 1.3
Intensity Change (Log2) – – – -0.0 -0.02 -0.22 – -0.19 -0.28 -0.35 -0.55 -0.33 -0.0 0.1 0.12 -0.42 -0.2 0.07 0.19 0.21 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.1 -5.9 -4.5 – -4.1 -3.7 -2.5 -2.9 -3.6 2.2 3.3 1.4 -2.6 -3.8 -0.5
Bud 0.9 0.5 -0.4 – -0.5 0.3 -0.4 -0.9 -0.9 0.8 -1.7 -1.4 -0.2 -1.9 -0.9
Bud Neck 0 0 2.3 – 0 0 0 0 1.9 0 0 0 0 4.1 0
Bud Site -0.9 -1.7 -0.3 – -4.3 -2.6 -1.6 -1.5 -2.9 -1.4 -1.4 -3.6 0.6 -0.3 0.2
Cell Periphery 2.6 -2.5 -0.8 – 0.4 2.2 2.3 1.8 2.4 0.1 -0.2 0.4 0.1 -2.1 -2.1
Cytoplasm -0.8 1.6 0.7 – -1.0 -2.5 -3.2 -2.3 -3.2 2.8 3.4 6.4 3.4 3.7 1.6
Endoplasmic Reticulum 1.8 1.2 2.5 – 2.4 2.3 2.4 2.8 3.3 0 2.3 0 2.6 2.7 2.0
Endosome -1.4 2.0 0.3 – 1.4 -1.7 -1.1 -5.1 -3.5 -2.6 -3.6 -4.3 -2.5 -2.7 -2.2
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.0 -0.1 5.9 – 5.3 11.1 10.9 14.7 11.3 0 0 0 2.1 4.6 2.2
Nucleus 0 0 0 – 1.4 0 0 0 0 0 0 0 0 1.1 2.7
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.5 2.0 1.7 – 2.2 1.4 1.0 0.6 2.0 0 0 0 0 0 0
Peroxisomes -0.6 -2.3 -3.5 – -5.5 -4.7 -3.5 -6.7 -5.0 -5.9 -8.9 -8.2 -3.4 -4.6 -5.4
SpindlePole -1.0 2.1 -3.0 – 1.0 -1.5 -3.9 -3.6 -2.8 -0.2 1.0 -1.0 0.3 3.5 1.3
Vacuole 0 1.8 0 – 3.4 2.4 2.2 0 2.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7666 2.8794 2.8601 2.7732 2.7395 2.9622 1.8343 2.4606 2.04 1.9752 1.3806 2.2186 1.247 0.4907 0.7536 0.5777 0.9288 0.851
Actin 0.2062 0.0839 0.092 0.1957 0.4667 0.104 0.1905 0.0308 0.0934 0.2727 0.4303 0.144 0.0852 0.052 0.095 0.2944 0.5446 0.0846
Bud 0.0567 0.0495 0.3287 0.1635 0.0716 0.0142 0.0454 0.0937 0.3305 0.1014 0.1049 0.0126 0.0021 0.0892 0.2607 0.0905 0.0574 0.0167
Bud Neck 0.0495 0.0062 0.0099 0.0052 0.0635 0.6814 0.0732 0.0201 0.0045 0.0335 0.0031 0.6983 0.0029 0.0019 0.0005 0.0001 0.0133 0.7052
Bud Periphery 0.1229 0.0471 0.4274 0.4335 0.225 0.0378 0.0707 0.0463 0.4494 0.3571 0.2651 0.0221 0.0019 0.0577 0.4196 0.5487 0.1643 0.0709
Bud Site 0.2255 0.4753 0.0778 0.0261 0.0345 0.0275 0.2712 0.5216 0.0772 0.0635 0.0297 0.0119 0.0193 0.3303 0.0126 0.0023 0.1466 0.003
Cell Periphery 0.0272 0.0089 0.0038 0.0032 0.0188 0.0076 0.0162 0.0043 0.0041 0.0046 0.0066 0.0027 0.0057 0.0029 0.0015 0.0034 0.0173 0.0039
Cytoplasm 0.213 0.2337 0.0278 0.0576 0.0887 0.0551 0.1261 0.2011 0.022 0.0556 0.0619 0.0394 0.3269 0.4097 0.0958 0.0378 0.0506 0.0307
Cytoplasmic Foci 0.0414 0.029 0.0127 0.0296 0.0051 0.0488 0.039 0.0118 0.0051 0.0232 0.0016 0.0322 0.2076 0.0034 0.0085 0.0006 0.0011 0.0718
Eisosomes 0.002 0.0011 0.0004 0.0002 0.0018 0.0004 0.0029 0.0004 0.0001 0.0009 0.0006 0.0002 0.0005 0.0001 0.0004 0.0002 0.0008 0.0003
Endoplasmic Reticulum 0.0078 0.0018 0.0005 0.0023 0.0011 0.0008 0.0034 0.0048 0.0003 0.0021 0.0005 0.0009 0.0229 0.019 0.0173 0.0025 0.0004 0.0007
Endosome 0.0083 0.0079 0.0012 0.0263 0.0025 0.0022 0.0143 0.0042 0.0007 0.0076 0.0008 0.0042 0.1377 0.0031 0.0143 0.0028 0.0002 0.0032
Golgi 0.008 0.0023 0.0008 0.0062 0.0035 0.0034 0.0083 0.002 0.0008 0.0047 0.0034 0.0026 0.0444 0.0009 0.0106 0.0011 0.0013 0.0017
Lipid Particles 0.006 0.0005 0.0003 0.0006 0.0002 0.001 0.0092 0.0006 0.0007 0.0061 0.0006 0.0046 0.0587 0.0004 0.0225 0.0001 0.0003 0.0005
Mitochondria 0.0016 0.0005 0.0008 0.004 0.0023 0.0004 0.0054 0.0003 0.0007 0.0018 0.0306 0.0004 0.0077 0.0003 0.0096 0.0014 0.0003 0.0005
None 0.0105 0.0388 0.0093 0.0044 0.0071 0.0021 0.0843 0.0309 0.0042 0.0437 0.0564 0.0022 0.008 0.0236 0.0201 0.0029 0.0003 0.0011
Nuclear Periphery 0.0021 0.0005 0.0002 0.0012 0.0005 0.0002 0.003 0.0004 0.0002 0.003 0.0002 0.0004 0.0147 0.0025 0.0026 0.0013 0.0001 0.0002
Nucleolus 0.0013 0.002 0.0003 0.0009 0.0006 0.0003 0.0027 0.0089 0.0003 0.0003 0.0002 0.0005 0.003 0.0001 0.0003 0.0001 0.0002 0.0001
Nucleus 0.001 0.0059 0.0006 0.002 0.0014 0.0008 0.0042 0.0038 0.0005 0.0007 0.0004 0.0005 0.0032 0.0009 0.0009 0.0005 0.0002 0.0003
Peroxisomes 0.003 0.0007 0.001 0.0027 0.001 0.01 0.0132 0.0008 0.0021 0.0089 0.0017 0.0172 0.0125 0.0001 0.0018 0.0001 0.0003 0.0018
Punctate Nuclear 0.0022 0.0015 0.0012 0.0017 0.0021 0.001 0.0133 0.0118 0.002 0.0061 0.0007 0.0018 0.0253 0.0005 0.0006 0.0001 0.0001 0.0021
Vacuole 0.0036 0.0027 0.0031 0.0294 0.0018 0.0012 0.0028 0.0012 0.001 0.0022 0.0006 0.0011 0.0067 0.0014 0.0035 0.007 0.0006 0.0003
Vacuole Periphery 0.0004 0.0003 0.0002 0.0039 0.0003 0.0001 0.0005 0.0001 0.0001 0.0005 0.0001 0.0001 0.003 0.0002 0.0013 0.0022 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.811 16.2411 24.1032 27.9656 21.7235 24.6141 24.0847 27.9546 24.8129 30.544
Translational Efficiency 0.5867 0.8457 0.5355 0.4829 0.537 0.4722 0.5702 0.4151 0.5046 0.4418

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
Localization
Cell Percentages bud neck (13%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sec15

Sec15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available