Standard name | |
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Human Ortholog | |
Description | Enzyme of the 'de novo' purine nucleotide biosynthetic pathway; contains aminoimidazole ribotide synthetase and glycinamide ribotide synthetase activities |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 5 | – | 7 | 14 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | – | 0 | 2 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 23 | 7 | 28 | 26 | 36 | 44 | 35 | – | 42 | 92 | 5 | 3 | 2 | 14 | 10 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 161 | 270 | 449 | 369 | 557 | 441 | 587 | 549 | 432 | – | 383 | 488 | 129 | 268 | 269 | 765 | 526 | 444 | 157 | 277 | 442 | 84 | 214 | 219 |
Endoplasmic Reticulum | 0 | 2 | 1 | 8 | 24 | 21 | 55 | 84 | 45 | – | 34 | 48 | 1 | 3 | 0 | 26 | 24 | 30 | 0 | 0 | 1 | 2 | 6 | 7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 7 | 1 | – | 3 | 12 | 0 | 1 | 2 | 3 | 1 | 5 | 0 | 3 | 2 | 0 | 1 | 3 |
Unique Cell Count | 161 | 274 | 450 | 375 | 584 | 470 | 650 | 652 | 500 | 443 | 598 | 132 | 271 | 272 | 769 | 528 | 449 | 160 | 286 | 453 | 99 | 234 | 251 | |
Labelled Cell Count | 161 | 274 | 473 | 384 | 610 | 492 | 683 | 689 | 528 | 473 | 661 | 137 | 276 | 274 | 813 | 562 | 492 | 160 | 286 | 453 | 99 | 234 | 251 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 103.4 | 108.5 | 76.3 | 77.4 | 64.2 | 53.9 | 47.6 | 45.1 | 41.2 | – | 37.0 | 36.7 | 115.6 | 124.1 | 110.4 | 152.9 | 156.5 | 154.4 | 89.9 | 93.8 | 97.8 |
Std Deviation (1e-4) | 26.2 | 30.0 | 20.2 | 21.8 | 17.3 | 14.7 | 13.4 | 11.4 | 11.0 | – | 10.2 | 9.6 | 39.7 | 39.0 | 40.8 | 42.9 | 35.3 | 40.0 | 32.8 | 32.2 | 31.9 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.25 | -0.5 | -0.68 | -0.76 | -0.89 | – | -1.04 | -1.06 | 0.6 | 0.7 | 0.53 | 1.0 | 1.04 | 1.02 | 0.24 | 0.3 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.5 | -0.2 | 0.3 | 0.3 | 1.1 | 1.2 | – | 2.5 | 5.3 | -0.6 | -2.8 | -3.1 | -3.2 | -2.8 | -1.9 |
Cytoplasm | -2.1 | -4.3 | -5.1 | -6.6 | -8.7 | -7.9 | – | -7.9 | -9.5 | -2.5 | -1.5 | -1.5 | -0.8 | -0.4 | -1.6 |
Endoplasmic Reticulum | 0 | 4.0 | 4.2 | 6.1 | 7.7 | 6.3 | – | 5.7 | 5.9 | 0 | 0 | 0 | 3.6 | 4.3 | 5.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 100.969 | 142.0612 | 130.1976 | 110.5906 | 80.5769 | 134.5292 | 128.179 | 161.862 | 147.5065 | 151.0678 | 133.3983 | 161.169 | 115.0842 | 163.8874 | 145.8841 | 144.6332 | 109.8632 | 114.5274 |
Actin | 0.0032 | 0.0004 | 0.0029 | 0.0087 | 0.0218 | 0.0006 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0107 | 0.0002 | 0.0006 | 0.0002 | 0.0216 | 0.0219 |
Bud | 0.0009 | 0.0013 | 0.0002 | 0.0003 | 0.0012 | 0.0001 | 0.0019 | 0.0001 | 0.0001 | 0.001 | 0.0013 | 0.0003 | 0.0016 | 0 | 0.0001 | 0.0004 | 0.0076 | 0.0007 |
Bud Neck | 0.0005 | 0.0003 | 0.0004 | 0.0004 | 0.0023 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0014 | 0.0017 |
Bud Periphery | 0.0007 | 0.002 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0008 | 0 | 0.0001 | 0.0012 | 0.0004 | 0.0001 | 0.0011 | 0 | 0 | 0 | 0.0236 | 0.0004 |
Bud Site | 0.0015 | 0.0003 | 0.0003 | 0.0002 | 0.0013 | 0 | 0.001 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0016 | 0.0001 | 0.0001 | 0 | 0.0082 | 0.0005 |
Cell Periphery | 0.0014 | 0.0026 | 0.0002 | 0.0004 | 0.0012 | 0.0001 | 0.0006 | 0.0009 | 0.0005 | 0.0002 | 0.0011 | 0.0001 | 0.0004 | 0.0005 | 0 | 0.0001 | 0.0021 | 0.0003 |
Cytoplasm | 0.8749 | 0.9723 | 0.9309 | 0.8741 | 0.73 | 0.9859 | 0.9515 | 0.9941 | 0.9731 | 0.9859 | 0.8986 | 0.994 | 0.8742 | 0.9967 | 0.984 | 0.9893 | 0.8297 | 0.8847 |
Cytoplasmic Foci | 0.0207 | 0.0029 | 0.015 | 0.0117 | 0.0184 | 0.0004 | 0.0029 | 0.0001 | 0.0072 | 0.0002 | 0.011 | 0.0006 | 0.0058 | 0.0001 | 0.0003 | 0.0005 | 0.0007 | 0.0026 |
Eisosomes | 0.0007 | 0 | 0.0001 | 0.0005 | 0.0065 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0 | 0 | 0.0006 | 0 |
Endoplasmic Reticulum | 0.0022 | 0.0006 | 0.0006 | 0.0012 | 0.0087 | 0.0004 | 0.0029 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0054 | 0.0001 | 0.001 | 0.0001 | 0.0019 | 0.0148 |
Endosome | 0.0085 | 0.0002 | 0.0047 | 0.0101 | 0.0276 | 0.0002 | 0.0037 | 0 | 0.0004 | 0.0002 | 0.0025 | 0.0003 | 0.0085 | 0 | 0.0019 | 0.0002 | 0.0089 | 0.0131 |
Golgi | 0.0035 | 0 | 0.0022 | 0.0136 | 0.0083 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0.0028 | 0 | 0.0015 | 0 | 0.0001 | 0 | 0.0029 | 0.0013 |
Lipid Particles | 0.0076 | 0.0003 | 0.0134 | 0.0179 | 0.0243 | 0 | 0.0017 | 0.0001 | 0.0022 | 0 | 0.0138 | 0 | 0.0014 | 0 | 0.0001 | 0 | 0.0019 | 0.0005 |
Mitochondria | 0.0156 | 0.0004 | 0.0021 | 0.0214 | 0.0246 | 0.0001 | 0.0049 | 0.0001 | 0.0019 | 0.0002 | 0.0161 | 0.0001 | 0.0059 | 0 | 0.0002 | 0.0001 | 0.0356 | 0.0015 |
None | 0.0087 | 0.0055 | 0.0026 | 0.0013 | 0.0348 | 0.002 | 0.0031 | 0.0004 | 0.0041 | 0.0002 | 0.0136 | 0.0003 | 0.0066 | 0.0001 | 0.0005 | 0.0002 | 0.0011 | 0.0011 |
Nuclear Periphery | 0.0085 | 0.0011 | 0.0073 | 0.003 | 0.019 | 0.0015 | 0.0054 | 0.0005 | 0.0012 | 0.0012 | 0.0015 | 0.0004 | 0.0324 | 0.0006 | 0.0054 | 0.0018 | 0.0072 | 0.0253 |
Nucleolus | 0.0136 | 0.0003 | 0.0004 | 0.0211 | 0.0092 | 0 | 0.0039 | 0.0004 | 0.0021 | 0 | 0.0066 | 0 | 0.0035 | 0 | 0.0001 | 0.0001 | 0.0031 | 0.0002 |
Nucleus | 0.0129 | 0.0015 | 0.003 | 0.0034 | 0.0119 | 0.0064 | 0.0053 | 0.0009 | 0.0018 | 0.0023 | 0.0061 | 0.0012 | 0.0274 | 0.0009 | 0.0044 | 0.0041 | 0.0073 | 0.0208 |
Peroxisomes | 0.0016 | 0 | 0.0059 | 0.0024 | 0.0067 | 0 | 0.0004 | 0 | 0.0008 | 0 | 0.0033 | 0 | 0.0005 | 0 | 0 | 0 | 0.0007 | 0.0001 |
Punctate Nuclear | 0.001 | 0.0001 | 0.0023 | 0.001 | 0.0289 | 0.0004 | 0.0004 | 0 | 0.0007 | 0 | 0.0004 | 0 | 0.0024 | 0 | 0.0004 | 0.0001 | 0.0003 | 0.0015 |
Vacuole | 0.0083 | 0.007 | 0.0032 | 0.004 | 0.0068 | 0.001 | 0.0058 | 0.0016 | 0.0018 | 0.0055 | 0.0108 | 0.0013 | 0.0047 | 0.0003 | 0.0004 | 0.0019 | 0.019 | 0.0045 |
Vacuole Periphery | 0.0036 | 0.0008 | 0.0018 | 0.0034 | 0.0052 | 0.0002 | 0.0022 | 0.0001 | 0.0007 | 0.0013 | 0.008 | 0.0002 | 0.0034 | 0 | 0.0002 | 0.0007 | 0.0146 | 0.0023 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 294.974 | 233.3891 | 172.881 | 162.5438 | 164.7965 | 247.2016 | 198.7196 | 186.7933 | 176.7042 | 178.0573 |
Translational Efficiency | 1.5556 | 1.5141 | 1.5703 | 1.4014 | 1.4064 | 1.694 | 1.4397 | 1.3277 | 1.3081 | 1.3138 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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