Standard name | |
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Human Ortholog | |
Description | Processivity factor; required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 7 | 3 |
Bud | 0 | 0 | 0 | 0 | 1 | 5 | 3 | 3 | 3 | 3 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 5 | 3 | 11 | 9 |
Bud Neck | 0 | 0 | 1 | 3 | 4 | 9 | 7 | 8 | 3 | 8 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 6 | 5 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 7 | 9 | 7 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 58 | 90 | 133 | 100 | 104 | 129 | 115 | 118 | 96 | 149 | 119 | 149 | 40 | 90 | 91 | 56 | 49 | 41 | 56 | 73 | 84 | 48 | 93 | 80 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 10 | 11 | 3 | 0 | 1 | 1 | 0 | 1 | 1 |
Endosome | 3 | 0 | 7 | 5 | 9 | 11 | 6 | 1 | 0 | 1 | 0 | 0 | 4 | 5 | 4 | 14 | 8 | 14 | 3 | 2 | 3 | 1 | 3 | 4 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 8 | 2 |
Mitochondria | 27 | 87 | 6 | 22 | 16 | 98 | 113 | 88 | 294 | 270 | 235 | 239 | 1 | 5 | 4 | 6 | 4 | 1 | 7 | 15 | 22 | 7 | 27 | 20 |
Nucleus | 97 | 51 | 128 | 123 | 161 | 174 | 147 | 164 | 140 | 166 | 106 | 96 | 78 | 103 | 109 | 39 | 37 | 34 | 122 | 93 | 176 | 55 | 105 | 79 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 1 | 1 | 0 | 1 | 3 | 2 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 0 | 2 | 2 | 4 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
SpindlePole | 0 | 1 | 4 | 10 | 17 | 16 | 13 | 8 | 4 | 10 | 4 | 14 | 0 | 1 | 1 | 0 | 0 | 0 | 11 | 8 | 28 | 10 | 20 | 27 |
Vac/Vac Membrane | 48 | 33 | 37 | 17 | 34 | 24 | 23 | 25 | 9 | 11 | 18 | 22 | 18 | 14 | 23 | 71 | 73 | 46 | 42 | 53 | 73 | 29 | 63 | 53 |
Unique Cell Count | 210 | 220 | 301 | 257 | 319 | 402 | 372 | 366 | 413 | 514 | 375 | 411 | 133 | 211 | 224 | 163 | 154 | 126 | 261 | 264 | 418 | 173 | 367 | 308 |
Labelled Cell Count | 234 | 263 | 319 | 281 | 347 | 474 | 432 | 420 | 558 | 631 | 499 | 544 | 141 | 219 | 234 | 198 | 182 | 142 | 261 | 264 | 418 | 173 | 367 | 308 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 3.5 | 5.3 | 4.9 | 5.7 | 4.4 | 4.6 | 4.7 | 3.2 | 3.7 | 3.6 | 3.6 | 5.2 | 5.1 | 5.1 | 6.5 | 8.0 | 9.2 | 5.8 | 6.2 | 6.0 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.3 | 1.4 | 2.4 | 1.2 | 2.2 | 1.5 | 0.7 | 0.8 | 1.5 | 1.4 | 1.4 | 1.2 | 1.3 | 1.5 | 1.9 | 2.6 | 2.4 | 2.5 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.1 | -0.28 | -0.22 | -0.18 | -0.72 | -0.53 | -0.57 | -0.55 | -0.02 | -0.06 | -0.07 | 0.3 | 0.59 | 0.79 | 0.13 | 0.23 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 7.6 | 8.3 | 5.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5695 | 2.6685 | 2.4658 | 2.0952 | 1.5891 | 3.021 | 4.421 | 3.5442 | 3.1375 | 3.449 | 4.4169 | 3.5737 | 2.1979 | 1.2316 | 1.3118 | 0.4816 | 1.2791 | 1.3088 |
Actin | 0.0433 | 0.0004 | 0.0004 | 0.0015 | 0.0002 | 0.0105 | 0.0416 | 0.0018 | 0.0086 | 0.0002 | 0.0089 | 0.0025 | 0.0231 | 0.0049 | 0.0024 | 0.0013 | 0.0003 | 0.0075 |
Bud | 0.0006 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.001 | 0.0026 | 0.0007 | 0 | 0.0011 | 0.0002 | 0.0004 | 0.0016 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0099 | 0.0009 | 0.0005 | 0.0015 | 0.0013 | 0.0171 | 0.0016 | 0.0006 | 0.0007 | 0.0003 | 0.0028 | 0.003 | 0.0071 | 0.0009 | 0.0003 | 0.0005 | 0.006 | 0.0038 |
Bud Periphery | 0.0007 | 0.0001 | 0 | 0.0002 | 0.0004 | 0.0008 | 0.0022 | 0.0064 | 0.0007 | 0 | 0.0021 | 0.0006 | 0.0006 | 0.0038 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.004 | 0.0023 | 0.0004 | 0.0007 | 0.0006 | 0.0015 | 0.0075 | 0.0064 | 0.0024 | 0.0001 | 0.0019 | 0.0003 | 0.0064 | 0.0046 | 0.0004 | 0.0003 | 0.0004 | 0.0003 |
Cell Periphery | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0011 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.1466 | 0.1574 | 0.0761 | 0.1669 | 0.2356 | 0.1399 | 0.1351 | 0.1652 | 0.1227 | 0.079 | 0.1313 | 0.1664 | 0.2288 | 0.1402 | 0.1055 | 0.2502 | 0.2057 | 0.2057 |
Cytoplasmic Foci | 0.0276 | 0.0037 | 0.0248 | 0.0022 | 0.0027 | 0.0214 | 0.068 | 0.0142 | 0.0203 | 0.0037 | 0.0108 | 0.0071 | 0.0409 | 0.0031 | 0.0037 | 0.0035 | 0.0033 | 0.0036 |
Eisosomes | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0094 | 0.0123 | 0.0096 | 0.0053 | 0.0021 | 0.0051 | 0.013 | 0.0071 | 0.0033 | 0.0011 | 0.0127 | 0.0036 | 0.0181 | 0.0069 | 0.0065 | 0.0026 | 0.0083 | 0.0019 |
Endosome | 0.0204 | 0.0038 | 0.0046 | 0.0025 | 0.0028 | 0.0208 | 0.0384 | 0.0055 | 0.017 | 0.0013 | 0.0181 | 0.0051 | 0.0199 | 0.0037 | 0.013 | 0.0028 | 0.0084 | 0.0027 |
Golgi | 0.0089 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0153 | 0.0292 | 0.0018 | 0.0048 | 0 | 0.0163 | 0.0004 | 0.0038 | 0.0008 | 0.0112 | 0.0001 | 0.0002 | 0.0014 |
Lipid Particles | 0.0105 | 0.0008 | 0.0039 | 0.0001 | 0.0002 | 0.0051 | 0.0169 | 0.0056 | 0.0117 | 0.001 | 0.0087 | 0.0014 | 0.0127 | 0.0007 | 0.0036 | 0.0001 | 0.0002 | 0.0032 |
Mitochondria | 0.01 | 0.0005 | 0.0008 | 0.0012 | 0.0004 | 0.0035 | 0.0231 | 0.0042 | 0.0032 | 0.0006 | 0.06 | 0.0145 | 0.0025 | 0.002 | 0.0057 | 0.0015 | 0.0013 | 0.0007 |
None | 0.257 | 0.1794 | 0.145 | 0.109 | 0.1625 | 0.2131 | 0.2654 | 0.385 | 0.2458 | 0.2322 | 0.3961 | 0.2121 | 0.1488 | 0.3021 | 0.3255 | 0.3774 | 0.2351 | 0.2537 |
Nuclear Periphery | 0.0165 | 0.0269 | 0.0193 | 0.0314 | 0.0072 | 0.0164 | 0.0235 | 0.0383 | 0.0182 | 0.0086 | 0.0286 | 0.0144 | 0.0635 | 0.0168 | 0.0324 | 0.0123 | 0.0177 | 0.0071 |
Nucleolus | 0.0048 | 0.0053 | 0.0033 | 0.0022 | 0.004 | 0.0086 | 0.003 | 0.0064 | 0.0038 | 0.0022 | 0.006 | 0.0075 | 0.0144 | 0.0053 | 0.0016 | 0.0019 | 0.0051 | 0.0028 |
Nucleus | 0.3542 | 0.5727 | 0.6887 | 0.6426 | 0.5123 | 0.4536 | 0.2475 | 0.277 | 0.491 | 0.6484 | 0.2547 | 0.498 | 0.3314 | 0.4679 | 0.4738 | 0.3246 | 0.4851 | 0.4613 |
Peroxisomes | 0.0229 | 0.0001 | 0.0069 | 0.0002 | 0.0001 | 0.0088 | 0.0444 | 0.0307 | 0.0138 | 0.0001 | 0.0078 | 0.0115 | 0.0241 | 0.0003 | 0.0007 | 0.0004 | 0.0001 | 0.004 |
Punctate Nuclear | 0.0461 | 0.0288 | 0.0129 | 0.0307 | 0.029 | 0.0505 | 0.028 | 0.0366 | 0.0279 | 0.0204 | 0.0263 | 0.0486 | 0.0421 | 0.0314 | 0.0097 | 0.019 | 0.018 | 0.0386 |
Vacuole | 0.0047 | 0.0034 | 0.0015 | 0.001 | 0.0367 | 0.0051 | 0.0058 | 0.0033 | 0.0023 | 0.0006 | 0.0038 | 0.002 | 0.0067 | 0.002 | 0.0019 | 0.0009 | 0.0041 | 0.0011 |
Vacuole Periphery | 0.001 | 0.0004 | 0.0004 | 0.0004 | 0.0006 | 0.0017 | 0.0035 | 0.0009 | 0.0007 | 0.0001 | 0.0014 | 0.0005 | 0.0028 | 0.0005 | 0.0013 | 0.0003 | 0.0006 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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