Standard name
Human Ortholog
Description Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.81 0.67 0.65 0.44 0.52 0.52 0.58 0.47 0.51 0.45 0.43 0.41 0.5 0.59 0.6 0.44 0.53 0.68 0.69 0.49 0.56 0.52 0.45 0.37
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.35 0.11 0.33 0.25 0.38 0.55 0.58 0.61 0.63 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Nucleus 0.08 0.11 0.07 0.15 0.1 0.18 0.17 0.18 0.11 0.17 0.1 0.13 0.12 0.1 0 0.06 0.15 0 0.18 0.27 0.2 0.25 0.25 0.27
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.34 0.39 0.31 0.24 0.37 0.16 0.18 0.17 0.1 0.12 0.13 0.13 0.53 0.44 0.46 0.53 0.53 0.55 0.05 0.12 0.15 0.13 0.13 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 2
Bud 0 0 2 5 1 1 3 7 5 9 8 5 0 0 3 0 0 0 2 7 1 6 9 15
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 2 3 1 2 1 3
Bud Site 0 0 1 1 0 4 0 4 4 2 2 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 1 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 185 231 210 129 179 217 309 276 146 192 139 178 81 155 163 27 21 30 164 198 233 112 89 92
Endoplasmic Reticulum 3 7 1 0 0 0 0 2 1 2 0 0 0 0 0 2 3 1 0 1 4 1 2 2
Endosome 0 0 4 2 15 3 5 2 0 0 0 0 1 2 2 1 1 1 4 5 1 3 3 3
Golgi 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 1 1
Mitochondria 0 11 15 101 37 138 133 220 160 251 201 271 1 0 6 4 0 2 0 10 7 1 3 1
Nucleus 19 39 24 44 36 74 89 103 32 72 34 55 19 25 11 4 6 1 42 109 84 53 49 69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 5 11 1 4 5
Vac/Vac Membrane 78 133 100 70 127 67 98 99 28 50 42 57 86 116 126 33 21 24 12 49 61 28 26 47
Unique Cell Count 229 345 323 290 343 421 531 583 289 430 327 430 162 262 271 62 40 44 237 403 418 216 200 252
Labelled Cell Count 286 423 357 352 395 507 637 716 376 579 428 571 188 298 312 73 52 59 237 403 418 216 200 252


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 5.1 4.7 4.4 4.9 4.4 4.3 4.2 3.6 3.8 3.7 3.6 6.1 6.4 5.5 7.9 7.9 9.7 6.2 6.6 7.0
Std Deviation (1e-4) 0.8 0.7 1.1 1.0 1.3 1.1 1.5 0.8 0.8 1.6 1.1 1.0 1.0 1.5 1.2 2.0 1.0 2.5 1.3 1.5 2.0
Intensity Change (Log2) – – – -0.08 0.07 -0.09 -0.1 -0.14 -0.37 -0.31 -0.32 -0.35 0.39 0.46 0.25 0.76 0.76 1.06 0.42 0.51 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -5.1 -3.4 -3.7 -2.0 -5.1 -3.6 -5.5 -5.8 -6.4 -3.2 -1.5 -1.2 -3.2 -1.6 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -2.3 -3.5 -1.6 0 0 0
Nucleus 3.0 1.4 4.1 3.9 4.3 1.6 3.8 1.3 2.4 1.6 0.9 -1.7 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 1.7 -4.9 -4.2 -4.9 -6.5 -6.6 -5.6 -5.9 4.7 3.3 3.9 3.4 2.7 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6628 3.4463 3.1726 2.7824 2.4558 3.1392 1.2114 1.814 1.853 1.4261 1.4723 1.4823 5.024 6.0616 5.6757 5.5 5.1261 5.4458
Actin 0.0182 0.0005 0.013 0.0005 0.023 0.0029 0.0083 0.0017 0.0042 0.0006 0.0019 0.0005 0.009 0.0004 0.0096 0.0004 0.0005 0.0006
Bud 0.0017 0.0004 0.0009 0.0001 0.0049 0.0006 0.001 0.001 0.0006 0.0009 0.0012 0.0003 0.0017 0.0002 0.0003 0.0002 0.0001 0.0004
Bud Neck 0.0083 0.0006 0.0056 0.0013 0.0019 0.0091 0.0082 0.0008 0.002 0.0008 0.0061 0.0027 0.0011 0.001 0.0029 0.001 0.0011 0.0053
Bud Periphery 0.002 0.0002 0.001 0.0001 0.0164 0.0005 0.0012 0.0006 0.0007 0.0005 0.0022 0.0001 0.001 0.0001 0.0002 0.0001 0 0.0001
Bud Site 0.0059 0.0016 0.005 0.0004 0.0029 0.0013 0.0024 0.0019 0.0005 0.0004 0.0029 0.0002 0.0024 0.0017 0.0018 0.0003 0.0004 0.0002
Cell Periphery 0.0003 0.0001 0.0007 0.0002 0.0003 0.0001 0.0003 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.6092 0.6096 0.6318 0.7444 0.5242 0.5326 0.3857 0.4275 0.3694 0.4907 0.3921 0.3363 0.6135 0.5967 0.5997 0.6486 0.7775 0.5458
Cytoplasmic Foci 0.0198 0.0023 0.0118 0.0058 0.0212 0.0081 0.0049 0.003 0.002 0.0015 0.0192 0.0044 0.0157 0.0036 0.0046 0.0043 0.0037 0.0114
Eisosomes 0.0002 0 0.0003 0 0.0002 0 0.0002 0 0 0 0 0 0 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0134 0.0072 0.009 0.0213 0.0112 0.0052 0.0094 0.0043 0.0035 0.0072 0.004 0.0043 0.0072 0.0035 0.0047 0.0087 0.0057 0.0047
Endosome 0.0171 0.0031 0.0142 0.0217 0.0497 0.0124 0.0137 0.0042 0.0034 0.0038 0.0088 0.0059 0.0071 0.0038 0.0031 0.004 0.0038 0.0037
Golgi 0.005 0.0001 0.0067 0.0025 0.0197 0.0036 0.0011 0.0004 0.0002 0 0.0011 0.0001 0.0017 0.0001 0.0004 0.0001 0.0001 0.0002
Lipid Particles 0.0071 0.0001 0.0062 0.0184 0.0088 0.0019 0.0019 0.0004 0.0001 0 0.0013 0.0001 0.0007 0.0001 0.0015 0.0002 0 0.0003
Mitochondria 0.016 0.0006 0.0078 0.0012 0.0302 0.0065 0.0039 0.0007 0.0007 0.0005 0.002 0.0004 0.0012 0.0003 0.0004 0.0007 0.0004 0.0004
None 0.0488 0.0512 0.0417 0.0231 0.0444 0.0229 0.0191 0.0121 0.0121 0.0059 0.0053 0.006 0.0792 0.0406 0.09 0.1192 0.0226 0.0597
Nuclear Periphery 0.0179 0.0084 0.0126 0.0137 0.0217 0.0102 0.0507 0.0233 0.0307 0.0306 0.0141 0.0292 0.0177 0.0098 0.0089 0.0209 0.0113 0.0105
Nucleolus 0.0044 0.0005 0.0011 0.0008 0.0022 0.0007 0.0063 0.0009 0.0007 0.0005 0.003 0.0009 0.0007 0.0005 0.0005 0.0006 0.0002 0.0005
Nucleus 0.1771 0.3079 0.2085 0.1337 0.1707 0.3637 0.4519 0.5035 0.5592 0.4456 0.486 0.575 0.2152 0.3264 0.2522 0.1837 0.1662 0.3413
Peroxisomes 0.0104 0.0001 0.0105 0.0009 0.0149 0.0035 0.0004 0.0006 0.0001 0.0001 0.0027 0.0001 0.0079 0.0001 0.0105 0.0001 0.0001 0.0015
Punctate Nuclear 0.0113 0.0031 0.007 0.0045 0.0214 0.0104 0.0217 0.0098 0.0069 0.0062 0.0414 0.0307 0.014 0.0082 0.007 0.0041 0.0047 0.0116
Vacuole 0.0054 0.0023 0.004 0.0047 0.0068 0.0029 0.0056 0.0028 0.0021 0.0033 0.0037 0.0022 0.0024 0.0026 0.0013 0.0023 0.0012 0.0014
Vacuole Periphery 0.001 0.0003 0.0008 0.0009 0.0033 0.0007 0.0019 0.0005 0.0007 0.001 0.0007 0.0007 0.0007 0.0002 0.0002 0.0005 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.7882 11.7776 9.9533 17.7877 17.0819 17.9227 15.0508 15.4049 15.9076 22.0446
Translational Efficiency 0.9825 0.9041 1.2886 0.7259 0.9391 1.1867 0.8329 0.7168 0.7566 0.7746

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (59%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Kap114

Kap114


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kap114-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available