Standard name
Human Ortholog
Description Nuclear protein with decapping endonuclease activity; targets mRNAs with unmethylated 7-methylguanosine cap structures and 5'-triphosphates; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; relocalizes to the cytosol in response to hypoxia; homologous to human DOM3Z
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0 0 0 0 0 0 0 0 0.06 0.09 0.13 0.07 0.05 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.14 0.1 0.15 0.23 0.24 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.98 0.99 0.96 0.9 0.92 0.88 0.84 0.84 0.81 0.76 0.77 0.78 0.92 0.89 0.83 0.87 0.88 0.9 0.96 0.97 0.93 0.93 0.8 0.72
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.06 0.06 0.14 0.17 0.16 0.2 0.23 0.27 0.23 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 3 0 2 3 0 3 0 0 0 1 0 0 1 0 0 1 4 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2 0 5 3 4 5 4 8 0 0 1 0 2 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 6 20 16 16 12 13 10 10 8 8 38 40 72 18 20 18 0 0 0 0 0 1
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 6 1 4 0 0 0 0 0 2
Endosome 1 0 0 0 0 0 0 1 0 0 0 1 2 0 2 1 1 0 1 0 1 1 0 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 3 0 1 0
Mitochondria 9 1 2 1 13 52 103 55 76 145 103 88 2 1 2 7 5 7 1 1 3 0 3 5
Nucleus 283 271 364 300 420 506 621 454 408 472 328 480 561 400 447 237 343 319 275 270 375 298 326 305
Nuclear Periphery 1 0 0 1 1 3 8 7 9 3 2 4 1 0 2 1 0 5 0 0 0 0 0 1
Nucleolus 5 1 6 21 29 80 125 88 98 140 113 143 2 4 6 10 13 11 1 0 4 3 17 28
Peroxisomes 1 0 0 0 0 1 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 0 0 1 0 1 0 0 0 3 11 0 1 1 0 0 0 0 1 1
Vac/Vac Membrane 1 0 4 6 7 1 4 5 9 3 4 8 4 3 4 8 15 11 5 3 8 11 49 64
Unique Cell Count 290 274 380 332 456 574 736 538 501 619 424 613 608 449 541 272 388 354 288 279 403 319 410 422
Labelled Cell Count 304 275 383 351 489 659 881 627 616 782 562 744 610 452 550 290 402 376 288 279 403 319 410 422


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 13.0 10.1 9.6 8.5 6.9 6.6 6.8 6.4 6.3 5.8 6.3 11.4 11.3 10.8 11.5 11.7 10.1 11.1 11.1 12.4
Std Deviation (1e-4) 1.8 3.5 3.3 3.8 2.6 2.2 1.8 2.2 2.2 2.1 1.6 2.2 3.4 3.7 3.7 3.6 3.8 3.8 2.6 2.6 2.5
Intensity Change (Log2) – – – -0.07 -0.25 -0.54 -0.6 -0.56 -0.64 -0.68 -0.79 -0.67 0.18 0.17 0.1 0.19 0.22 0.01 0.15 0.14 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 1.7 1.2 0.1 0.9 0.5 0 0.3 -0.4 3.5 4.6 6.3 3.4 2.7 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.5 5.6 7.3 6.0 7.6 9.9 10.0 7.4 0 0 0 0 0 0
Nucleus -2.9 -2.2 -4.1 -5.6 -5.5 -6.4 -8.1 -7.5 -7.5 -2.2 -3.6 -6.1 -4.1 -3.8 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.3 3.4 6.5 7.6 7.3 8.2 9.1 10.0 9.3 0 0 0 1.7 1.6 1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.6 0 0 0 0.9 0 0 0 0 0 0 1.8 2.5 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4663 7.5249 7.1886 5.6054 6.4233 6.5554 5.4302 6.1546 5.6132 5.5742 3.8818 5.4384 4.952 5.6776 4.8348 5.3121 4.0509 4.5891
Actin 0.0018 0 0 0 0 0.0001 0.0185 0.0001 0.0157 0.0487 0.0034 0.001 0.0338 0 0.0001 0.0006 0.0001 0.0052
Bud 0 0 0 0 0 0 0.001 0.0002 0.0005 0.0006 0.0003 0.0001 0.001 0 0 0.0004 0.0001 0.0001
Bud Neck 0.0003 0 0 0 0.0001 0.0003 0.002 0.0001 0.0008 0.0007 0.0004 0.0003 0.0012 0 0.0001 0.0003 0.0003 0.0004
Bud Periphery 0 0 0 0 0 0 0.0021 0.0002 0.0009 0.002 0.0007 0.0002 0.002 0 0 0.0014 0.0001 0.0002
Bud Site 0.0002 0 0 0 0 0 0.0047 0.0015 0.005 0.0011 0.0006 0.0001 0.0032 0 0.0002 0.0007 0.0003 0.0002
Cell Periphery 0 0 0 0 0 0 0.0007 0.0001 0.0003 0.0005 0.0003 0.0001 0.0003 0 0 0.0007 0.0001 0.0001
Cytoplasm 0.0006 0.011 0 0 0 0 0.0045 0.0001 0.002 0.0001 0.0005 0.0001 0.0036 0 0.0006 0.0001 0.0016 0.0002
Cytoplasmic Foci 0.0007 0 0 0 0 0.0001 0.0201 0.0017 0.0167 0.0002 0.0048 0.0011 0.0097 0 0.0006 0.0001 0.0291 0.0009
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0002 0 0 0.0001 0 0.0001
Endoplasmic Reticulum 0.0003 0 0 0 0 0 0.002 0 0.0015 0.0001 0.0018 0.0003 0.0017 0 0.0002 0.0002 0.0002 0.0003
Endosome 0.0015 0 0 0 0 0.0004 0.0185 0.0001 0.0098 0.0002 0.0266 0.0021 0.011 0 0.0031 0.0007 0.0058 0.0024
Golgi 0.0003 0 0 0 0 0.0007 0.0042 0.0001 0.0036 0.0007 0.0096 0.001 0.0051 0 0.0002 0.0002 0.0003 0.0028
Lipid Particles 0.0003 0 0 0 0 0.0003 0.016 0.0006 0.015 0.0001 0.0575 0.0108 0.0081 0 0.0003 0.0006 0.0015 0.0046
Mitochondria 0.0001 0 0 0.0001 0.0002 0.0031 0.0132 0.0007 0.008 0.0047 0.0368 0.0015 0.0079 0.0001 0.001 0.0233 0.0017 0.0031
None 0.0006 0 0 0 0 0 0.0051 0 0.0009 0 0.001 0.0001 0.0047 0 0.0006 0.0001 0.001 0.0002
Nuclear Periphery 0.0009 0.0043 0.0003 0.0048 0.0007 0.0011 0.0155 0.0017 0.0077 0.0014 0.0254 0.0026 0.0086 0.0002 0.0037 0.0015 0.0024 0.0036
Nucleolus 0.0097 0.0083 0.0065 0.0143 0.0755 0.0446 0.0418 0.0111 0.0091 0.0129 0.0393 0.0259 0.0202 0.0108 0.0067 0.0162 0.0384 0.0294
Nucleus 0.9816 0.9758 0.9925 0.9708 0.9225 0.9482 0.7935 0.9765 0.8923 0.9224 0.7664 0.9498 0.8491 0.9888 0.9807 0.9456 0.8948 0.941
Peroxisomes 0.0001 0 0 0 0 0.0004 0.0122 0.0005 0.0045 0.0002 0.0072 0.0003 0.0109 0 0.0001 0.0003 0.0005 0.0016
Punctate Nuclear 0.0007 0.0005 0.0005 0.0099 0.0007 0.0004 0.0159 0.0005 0.0024 0.0001 0.0038 0.0012 0.0144 0.0001 0.0012 0.0001 0.0203 0.0029
Vacuole 0.0001 0 0 0 0.0001 0.0001 0.0049 0.001 0.0018 0.0015 0.0041 0.0007 0.002 0 0.0003 0.0035 0.0008 0.0004
Vacuole Periphery 0.0001 0 0 0.0001 0.0001 0.0001 0.0033 0.0034 0.0015 0.0016 0.0093 0.0008 0.0013 0 0.0002 0.0033 0.0007 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.9588 14.4058 17.6067 21.3762 20.2793 17.0535 20.5634 16.7703 17.9607 21.7716
Translational Efficiency 1.3377 1.5291 1.1722 0.9519 1.0411 1.2451 0.9087 1.0623 0.9075 0.9367

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nuclear protein with decapping endonuclease activity; targets mRNAs with unmethylated 7-methylguanosine cap structures and 5'-triphosphates; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; relocalizes to the cytosol in response to hypoxia; homologous to human DOM3Z
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Rai1

Rai1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rai1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available