Standard name | |
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Human Ortholog | |
Description | Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 5 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 6 | 8 | 5 | 7 | 13 |
Bud | 1 | 4 | 0 | 3 | 0 | 3 | 4 | – | 16 | 11 | 7 | 9 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 5 | 18 |
Bud Neck | 0 | 3 | 33 | 13 | 33 | 26 | 39 | – | 28 | 16 | 7 | 28 | 0 | 1 | 2 | 0 | 2 | 1 | 2 | 2 | 6 | 7 | 8 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 8 | 5 | 6 | 7 | 6 | – | 1 | 4 | 2 | 3 | 0 | 1 | 3 | 6 | 2 | 12 | 2 | 1 | 11 | 8 | 8 | 12 |
Cytoplasm | 216 | 197 | 441 | 428 | 635 | 595 | 741 | – | 526 | 463 | 357 | 473 | 493 | 592 | 732 | 446 | 230 | 225 | 174 | 175 | 257 | 180 | 276 | 352 |
Endoplasmic Reticulum | 3 | 1 | 0 | 1 | 0 | 1 | 1 | – | 0 | 0 | 0 | 4 | 1 | 0 | 5 | 46 | 18 | 16 | 4 | 0 | 2 | 5 | 7 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 2 | 0 | 1 | 5 | 2 | 3 | 3 | 4 | 10 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 13 | 10 | 0 | 7 | 5 | 48 | 72 | – | 184 | 128 | 178 | 136 | 7 | 3 | 3 | 14 | 3 | 1 | 52 | 31 | 93 | 88 | 91 | 98 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 0 | 2 | – | 2 | 4 | 4 | 4 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 1 | 3 | 1 | 7 | 7 | 19 | – | 17 | 15 | 13 | 46 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 8 | 1 | 7 | 10 |
Vac/Vac Membrane | 1 | 0 | 1 | 0 | 1 | 3 | 1 | – | 2 | 5 | 3 | 7 | 0 | 0 | 0 | 4 | 4 | 3 | 6 | 3 | 9 | 12 | 19 | 31 |
Unique Cell Count | 230 | 214 | 453 | 442 | 652 | 638 | 810 | 659 | 577 | 508 | 631 | 500 | 598 | 741 | 468 | 237 | 231 | 265 | 236 | 427 | 323 | 449 | 581 | |
Labelled Cell Count | 234 | 219 | 488 | 463 | 689 | 695 | 888 | 779 | 649 | 574 | 716 | 503 | 601 | 747 | 528 | 263 | 262 | 265 | 236 | 427 | 323 | 449 | 581 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.6 | 3.4 | 4.6 | 4.0 | 4.6 | 3.6 | 3.7 | – | 3.2 | 3.2 | 3.1 | 3.7 | 3.6 | 3.7 | 3.8 | 5.5 | 5.7 | 5.6 | 3.5 | 3.6 | 4.0 |
Std Deviation (1e-4) | 0.6 | 0.6 | 0.7 | 0.9 | 1.1 | 1.1 | 0.9 | – | 0.9 | 0.8 | 0.7 | 0.9 | 0.6 | 0.8 | 1.0 | 1.2 | 1.2 | 1.4 | 1.0 | 1.0 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.0 | -0.34 | -0.32 | – | -0.53 | -0.51 | -0.56 | -0.31 | -0.36 | -0.3 | -0.26 | 0.28 | 0.33 | 0.29 | -0.37 | -0.35 | -0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.0453 | -0.4652 | -0.6546 | -0.1565 | -1.0952 | -0.6651 | -0.442 | -0.7787 | -0.8177 | -0.3337 | -0.8495 | -1.0491 | -1.274 | -0.4946 | -0.7404 | -0.3886 | -1.102 | -0.7767 |
Actin | 0.0152 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0845 | 0.0017 | 0.0113 | 0.0481 | 0.0347 | 0.0022 | 0.0001 | 0.0019 | 0.0002 | 0.004 | 0.0022 | 0.0026 |
Bud | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0019 | 0.0019 | 0.0013 | 0.0029 | 0.0012 | 0.0011 | 0.0001 | 0.0006 | 0.0001 | 0.0037 | 0.0009 | 0.0002 |
Bud Neck | 0.0019 | 0 | 0.0013 | 0 | 0 | 0.0003 | 0.0055 | 0.0002 | 0.0004 | 0.0015 | 0.0045 | 0.0018 | 0.0001 | 0.0005 | 0.0004 | 0.0098 | 0.001 | 0.0092 |
Bud Periphery | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.004 | 0.0075 | 0.0039 | 0.0058 | 0.002 | 0.0036 | 0.0002 | 0.0006 | 0.0001 | 0.0076 | 0.0039 | 0.0004 |
Bud Site | 0.0022 | 0.0001 | 0.0113 | 0 | 0 | 0 | 0.0124 | 0.0078 | 0.0032 | 0.0118 | 0.0304 | 0.0001 | 0.0002 | 0.0059 | 0.0005 | 0.0052 | 0.0005 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0006 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0007 | 0.0002 | 0.0001 |
Cytoplasm | 0.0167 | 0.0297 | 0.0371 | 0.0509 | 0.0235 | 0.0475 | 0.063 | 0.1158 | 0.0903 | 0.1105 | 0.0963 | 0.1486 | 0.0544 | 0.1423 | 0.1156 | 0.0888 | 0.1534 | 0.1336 |
Cytoplasmic Foci | 0.0029 | 0.0024 | 0.0032 | 0.0015 | 0.0127 | 0.0043 | 0.0438 | 0.0101 | 0.0147 | 0.0168 | 0.0229 | 0.0172 | 0.0038 | 0.0218 | 0.0085 | 0.0081 | 0.0174 | 0.0148 |
Eisosomes | 0.0003 | 0.0002 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.001 | 0.0008 | 0.0006 | 0.0003 | 0.0005 | 0.0003 | 0.0034 | 0.0016 | 0.0026 | 0.0014 | 0.0005 | 0.0006 | 0.0016 | 0.0018 | 0.0011 | 0.0007 | 0.001 | 0.001 |
Endosome | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0277 | 0.0028 | 0.0137 | 0.0079 | 0.0084 | 0.0022 | 0.0008 | 0.005 | 0.0016 | 0.0012 | 0.0022 | 0.0026 |
Golgi | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.008 | 0.0017 | 0.0129 | 0.0123 | 0.0099 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0003 |
Lipid Particles | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0203 | 0.0008 | 0.003 | 0.0085 | 0.0023 | 0.0004 | 0.0006 | 0.0007 | 0.0003 | 0.0004 | 0.0006 | 0.0002 |
Mitochondria | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0104 | 0.0012 | 0.0142 | 0.0097 | 0.0243 | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0006 | 0.0006 | 0.0004 |
None | 0.954 | 0.9648 | 0.9424 | 0.9444 | 0.9598 | 0.9448 | 0.6706 | 0.8368 | 0.8116 | 0.7479 | 0.7493 | 0.8168 | 0.9356 | 0.8109 | 0.8681 | 0.8634 | 0.8125 | 0.8302 |
Nuclear Periphery | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0044 | 0.0006 | 0.0009 | 0.0007 | 0.0003 | 0.0005 | 0.0003 | 0.0006 | 0.0004 | 0.0004 | 0.0004 | 0.0003 |
Nucleolus | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0013 | 0.0008 | 0.0004 | 0.0003 | 0.0014 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0002 |
Nucleus | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0023 | 0.0013 | 0.0009 | 0.0005 | 0.0012 | 0.0005 | 0.0004 | 0.0011 | 0.0007 | 0.0013 | 0.0007 | 0.0011 |
Peroxisomes | 0.0011 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0228 | 0.0012 | 0.0057 | 0.0096 | 0.0065 | 0.0006 | 0.0001 | 0.0012 | 0.0002 | 0.0013 | 0.0004 | 0.0003 |
Punctate Nuclear | 0.001 | 0.0002 | 0.0008 | 0.0001 | 0.0009 | 0.0009 | 0.0075 | 0.0039 | 0.0039 | 0.0018 | 0.0013 | 0.0017 | 0.0004 | 0.0013 | 0.0006 | 0.001 | 0.0008 | 0.0017 |
Vacuole | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0032 | 0.0012 | 0.0036 | 0.001 | 0.0016 | 0.0008 | 0.0005 | 0.0011 | 0.0006 | 0.0008 | 0.0006 | 0.0004 |
Vacuole Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0016 | 0.0001 | 0.0006 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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