Standard name
Human Ortholog
Description Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.94 0.92 0.97 0.97 0.97 0.93 0.91 0.8 0.8 0.7 0.75 0.99 0.99 0.99 0.95 0.97 0.97 0.66 0.75 0.6 0.56 0.62 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.05 0 0 0 0.08 0.09 0.28 0.22 0.35 0.22 0 0 0 0 0 0 0.2 0.13 0.22 0.27 0.2 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 5 0 2 0 0 0 0 0 0 0 0 1 1 0 2 6 8 5 7 13
Bud 1 4 0 3 0 3 4 16 11 7 9 1 1 0 0 0 0 1 0 3 2 5 18
Bud Neck 0 3 33 13 33 26 39 28 16 7 28 0 1 2 0 2 1 2 2 6 7 8 10
Bud Site 0 0 0 0 0 0 1 2 1 2 1 0 0 1 0 0 0
Cell Periphery 0 3 8 5 6 7 6 1 4 2 3 0 1 3 6 2 12 2 1 11 8 8 12
Cytoplasm 216 197 441 428 635 595 741 526 463 357 473 493 592 732 446 230 225 174 175 257 180 276 352
Endoplasmic Reticulum 3 1 0 1 0 1 1 0 0 0 4 1 0 5 46 18 16 4 0 2 5 7 5
Endosome 0 0 0 0 0 1 1 0 0 0 3 0 2 0 2 0 1 5 2 3 3 4 10
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 3 2 1 1 0 0 0 0 1
Mitochondria 13 10 0 7 5 48 72 184 128 178 136 7 3 3 14 3 1 52 31 93 88 91 98
Nucleus 0 0 0 0 2 0 2 2 4 4 4 0 0 1 1 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 0 0 0 1 1 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 2 0 1 0 4 0 0 0 0 0 0 1 0
SpindlePole 0 1 3 1 7 7 19 17 15 13 46 1 0 0 0 0 1 3 4 8 1 7 10
Vac/Vac Membrane 1 0 1 0 1 3 1 2 5 3 7 0 0 0 4 4 3 6 3 9 12 19 31
Unique Cell Count 230 214 453 442 652 638 810 659 577 508 631 500 598 741 468 237 231 265 236 427 323 449 581
Labelled Cell Count 234 219 488 463 689 695 888 779 649 574 716 503 601 747 528 263 262 265 236 427 323 449 581


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 3.4 4.6 4.0 4.6 3.6 3.7 3.2 3.2 3.1 3.7 3.6 3.7 3.8 5.5 5.7 5.6 3.5 3.6 4.0
Std Deviation (1e-4) 0.6 0.6 0.7 0.9 1.1 1.1 0.9 0.9 0.8 0.7 0.9 0.6 0.8 1.0 1.2 1.2 1.4 1.0 1.0 1.4
Intensity Change (Log2) -0.2 -0.0 -0.34 -0.32 -0.53 -0.51 -0.56 -0.31 -0.36 -0.3 -0.26 0.28 0.33 0.29 -0.37 -0.35 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0453 -0.4652 -0.6546 -0.1565 -1.0952 -0.6651 -0.442 -0.7787 -0.8177 -0.3337 -0.8495 -1.0491 -1.274 -0.4946 -0.7404 -0.3886 -1.102 -0.7767
Actin 0.0152 0.0001 0.0003 0.0004 0.0002 0.0002 0.0845 0.0017 0.0113 0.0481 0.0347 0.0022 0.0001 0.0019 0.0002 0.004 0.0022 0.0026
Bud 0.0001 0 0.0003 0 0 0 0.0019 0.0019 0.0013 0.0029 0.0012 0.0011 0.0001 0.0006 0.0001 0.0037 0.0009 0.0002
Bud Neck 0.0019 0 0.0013 0 0 0.0003 0.0055 0.0002 0.0004 0.0015 0.0045 0.0018 0.0001 0.0005 0.0004 0.0098 0.001 0.0092
Bud Periphery 0.0001 0 0.0003 0.0001 0 0 0.004 0.0075 0.0039 0.0058 0.002 0.0036 0.0002 0.0006 0.0001 0.0076 0.0039 0.0004
Bud Site 0.0022 0.0001 0.0113 0 0 0 0.0124 0.0078 0.0032 0.0118 0.0304 0.0001 0.0002 0.0059 0.0005 0.0052 0.0005 0.0002
Cell Periphery 0.0001 0.0001 0.0002 0 0 0.0001 0.0006 0.0006 0.0004 0.0004 0.0002 0.0002 0.0002 0.0006 0.0001 0.0007 0.0002 0.0001
Cytoplasm 0.0167 0.0297 0.0371 0.0509 0.0235 0.0475 0.063 0.1158 0.0903 0.1105 0.0963 0.1486 0.0544 0.1423 0.1156 0.0888 0.1534 0.1336
Cytoplasmic Foci 0.0029 0.0024 0.0032 0.0015 0.0127 0.0043 0.0438 0.0101 0.0147 0.0168 0.0229 0.0172 0.0038 0.0218 0.0085 0.0081 0.0174 0.0148
Eisosomes 0.0003 0.0002 0.0004 0.0006 0.0001 0.0001 0.001 0.0002 0.0005 0.0003 0.0002 0.0001 0.0002 0.0004 0.0002 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.001 0.0008 0.0006 0.0003 0.0005 0.0003 0.0034 0.0016 0.0026 0.0014 0.0005 0.0006 0.0016 0.0018 0.0011 0.0007 0.001 0.001
Endosome 0.0007 0.0004 0.0002 0.0002 0.0006 0.0003 0.0277 0.0028 0.0137 0.0079 0.0084 0.0022 0.0008 0.005 0.0016 0.0012 0.0022 0.0026
Golgi 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.008 0.0017 0.0129 0.0123 0.0099 0.0002 0.0001 0.0007 0.0002 0.0003 0.0003 0.0003
Lipid Particles 0.0004 0.0003 0.0003 0.0002 0.0004 0.0001 0.0203 0.0008 0.003 0.0085 0.0023 0.0004 0.0006 0.0007 0.0003 0.0004 0.0006 0.0002
Mitochondria 0.0001 0.0001 0.0003 0.0004 0.0001 0.0001 0.0104 0.0012 0.0142 0.0097 0.0243 0.0005 0.0002 0.0004 0.0003 0.0006 0.0006 0.0004
None 0.954 0.9648 0.9424 0.9444 0.9598 0.9448 0.6706 0.8368 0.8116 0.7479 0.7493 0.8168 0.9356 0.8109 0.8681 0.8634 0.8125 0.8302
Nuclear Periphery 0.0005 0.0002 0.0002 0.0002 0.0002 0.0002 0.0044 0.0006 0.0009 0.0007 0.0003 0.0005 0.0003 0.0006 0.0004 0.0004 0.0004 0.0003
Nucleolus 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0013 0.0008 0.0004 0.0003 0.0014 0.0002 0.0001 0.0003 0.0002 0.0003 0.0002 0.0002
Nucleus 0.0009 0.0002 0.0003 0.0001 0.0001 0.0004 0.0023 0.0013 0.0009 0.0005 0.0012 0.0005 0.0004 0.0011 0.0007 0.0013 0.0007 0.0011
Peroxisomes 0.0011 0.0001 0.0003 0.0001 0.0005 0.0001 0.0228 0.0012 0.0057 0.0096 0.0065 0.0006 0.0001 0.0012 0.0002 0.0013 0.0004 0.0003
Punctate Nuclear 0.001 0.0002 0.0008 0.0001 0.0009 0.0009 0.0075 0.0039 0.0039 0.0018 0.0013 0.0017 0.0004 0.0013 0.0006 0.001 0.0008 0.0017
Vacuole 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0032 0.0012 0.0036 0.001 0.0016 0.0008 0.0005 0.0011 0.0006 0.0008 0.0006 0.0004
Vacuole Periphery 0 0 0 0 0 0 0.0016 0.0001 0.0006 0.0004 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
Localization
Cell Percentages cytoplasm (19%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Zip2

Zip2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Zip2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available