Standard name | |
---|---|
Human Ortholog | |
Description | Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | – | 0 | 0 | 0 | 6 | 7 | 0 | 5 | 1 | 2 | – | – | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 10 | – | 0 | 7 | 4 | 13 | 12 | 1 | 10 | 3 | 0 | – | – | – | 14 | 22 | 17 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 223 | 255 | – | 23 | 114 | 65 | 139 | 147 | 32 | 95 | 33 | 29 | – | – | – | 354 | 306 | 295 | 218 | 255 | 33 | 1 | 12 | 13 |
Endoplasmic Reticulum | 2 | 6 | – | 0 | 3 | 3 | 12 | 19 | 0 | 17 | 3 | 3 | – | – | – | 8 | 9 | 12 | 5 | 6 | 1 | 0 | 0 | 0 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 2 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | – | – | – | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | – | 0 | 0 | 0 | 6 | 4 | 6 | 12 | 6 | 11 | – | – | – | 3 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 1 |
Nucleus | 0 | 0 | – | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | – | 1 | 3 | 0 | 5 | 1 | 1 | 0 | 0 | 1 | – | – | – | 31 | 7 | 14 | 0 | 5 | 0 | 0 | 1 | 0 |
Unique Cell Count | 225 | 262 | 26 | 123 | 69 | 164 | 177 | 40 | 124 | 40 | 43 | 387 | 331 | 321 | 229 | 270 | 38 | 7 | 18 | 24 | ||||
Labelled Cell Count | 228 | 273 | 27 | 127 | 72 | 184 | 193 | 40 | 139 | 46 | 49 | 416 | 354 | 340 | 229 | 270 | 38 | 7 | 18 | 24 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.2 | 15.1 | – | 10.5 | 15.6 | 12.8 | 13.3 | 12.3 | 9.4 | 11.2 | 10.1 | 9.4 | – | – | – | 20.7 | 20.3 | 19.3 | – | – | – |
Std Deviation (1e-4) | 1.8 | 2.7 | – | 2.4 | 2.3 | 2.1 | 2.1 | 2.1 | 1.9 | 2.3 | 2.2 | 1.6 | – | – | – | 4.2 | 4.1 | 4.0 | – | – | – |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.4372 | 11.0378 | 9.3015 | 9.1487 | 7.5652 | 9.561 | 6.1814 | 9.6311 | 8.8504 | 8.4251 | 6.0034 | 9.031 | 7.4252 | 10.482 | 9.3531 | 7.5774 | 8.8297 | 9.4701 |
Actin | 0.0072 | 0.0011 | 0.0079 | 0.0054 | 0.0018 | 0.0012 | 0.0247 | 0.0027 | 0.0081 | 0.0026 | 0.0323 | 0.0012 | 0.0071 | 0.0003 | 0.0051 | 0.0097 | 0.0111 | 0.0055 |
Bud | 0.001 | 0.003 | 0.0031 | 0.001 | 0.0005 | 0.0036 | 0.0006 | 0.001 | 0.0005 | 0.0011 | 0.0005 | 0.0004 | 0.0007 | 0.0003 | 0.0024 | 0.0008 | 0.0005 | 0.0003 |
Bud Neck | 0.0009 | 0.0004 | 0.0014 | 0.0007 | 0.0012 | 0.0034 | 0.0011 | 0.0002 | 0.0004 | 0.0016 | 0.0019 | 0.0009 | 0.0012 | 0.0002 | 0.0023 | 0.001 | 0.0009 | 0.0008 |
Bud Periphery | 0.0009 | 0.0008 | 0.0023 | 0.0006 | 0.0006 | 0.0027 | 0.0007 | 0.0006 | 0.0002 | 0.0008 | 0.0008 | 0.0003 | 0.0009 | 0.0001 | 0.0047 | 0.0008 | 0.0007 | 0.0002 |
Bud Site | 0.0025 | 0.0021 | 0.0061 | 0.0007 | 0.0009 | 0.0005 | 0.0011 | 0.0011 | 0.0011 | 0.001 | 0.0037 | 0.0001 | 0.0014 | 0.0009 | 0.0026 | 0.0039 | 0.0008 | 0.0004 |
Cell Periphery | 0.0008 | 0.0004 | 0.0006 | 0.0001 | 0.0003 | 0.0005 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.7407 | 0.9417 | 0.8157 | 0.7957 | 0.6427 | 0.8722 | 0.7423 | 0.9438 | 0.8635 | 0.6957 | 0.5865 | 0.8379 | 0.8456 | 0.9674 | 0.8887 | 0.748 | 0.8541 | 0.883 |
Cytoplasmic Foci | 0.0223 | 0.0085 | 0.0316 | 0.017 | 0.051 | 0.0173 | 0.0295 | 0.0113 | 0.0153 | 0.0254 | 0.0312 | 0.0181 | 0.0209 | 0.0066 | 0.0132 | 0.0227 | 0.0164 | 0.0116 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0005 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0484 | 0.0157 | 0.0118 | 0.0224 | 0.005 | 0.0142 | 0.0403 | 0.005 | 0.0165 | 0.0141 | 0.0209 | 0.0431 | 0.0365 | 0.0047 | 0.0147 | 0.004 | 0.0212 | 0.0345 |
Endosome | 0.085 | 0.0151 | 0.0644 | 0.0999 | 0.1191 | 0.0507 | 0.0837 | 0.0224 | 0.0491 | 0.1051 | 0.1109 | 0.0545 | 0.0377 | 0.0131 | 0.0287 | 0.0831 | 0.0503 | 0.0358 |
Golgi | 0.018 | 0.0025 | 0.0139 | 0.0226 | 0.0422 | 0.0054 | 0.013 | 0.0029 | 0.0065 | 0.0144 | 0.0222 | 0.0088 | 0.01 | 0.0016 | 0.0026 | 0.0087 | 0.0033 | 0.0053 |
Lipid Particles | 0.0082 | 0.0001 | 0.0028 | 0.0003 | 0.02 | 0.0001 | 0.0061 | 0 | 0.0037 | 0.0143 | 0.0445 | 0.0008 | 0.0055 | 0 | 0.0017 | 0.0112 | 0.003 | 0.0028 |
Mitochondria | 0.0228 | 0.0012 | 0.0136 | 0.0054 | 0.0866 | 0.0134 | 0.0063 | 0.0011 | 0.0049 | 0.0416 | 0.0682 | 0.0246 | 0.0042 | 0.0003 | 0.0145 | 0.039 | 0.0012 | 0.0083 |
None | 0.0028 | 0.0007 | 0.001 | 0.0006 | 0.0011 | 0.0009 | 0.0074 | 0.0008 | 0.0035 | 0.0005 | 0.0032 | 0.0008 | 0.0057 | 0.0005 | 0.001 | 0.0011 | 0.0014 | 0.0017 |
Nuclear Periphery | 0.0062 | 0.0011 | 0.0015 | 0.0032 | 0.001 | 0.0011 | 0.0103 | 0.0006 | 0.002 | 0.0033 | 0.0066 | 0.0009 | 0.0039 | 0.0004 | 0.0018 | 0.0031 | 0.0183 | 0.0032 |
Nucleolus | 0.0015 | 0 | 0.0011 | 0 | 0.0005 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0003 | 0 | 0.0001 | 0.0007 | 0.0005 | 0.0001 |
Nucleus | 0.0028 | 0.0008 | 0.0025 | 0.0018 | 0.0012 | 0.0012 | 0.0028 | 0.0007 | 0.0012 | 0.0014 | 0.0021 | 0.0008 | 0.002 | 0.0005 | 0.0009 | 0.0012 | 0.0033 | 0.001 |
Peroxisomes | 0.0031 | 0.0001 | 0.0024 | 0.0001 | 0.0027 | 0.0002 | 0.0037 | 0.0001 | 0.0035 | 0.0152 | 0.0293 | 0.0003 | 0.0036 | 0 | 0.0027 | 0.0227 | 0.0007 | 0.0007 |
Punctate Nuclear | 0.0007 | 0.0001 | 0.0008 | 0.0001 | 0.0006 | 0.0001 | 0.0032 | 0.0001 | 0.0008 | 0.0005 | 0.0131 | 0.0001 | 0.003 | 0 | 0.0002 | 0.0004 | 0.0064 | 0.0006 |
Vacuole | 0.0192 | 0.0038 | 0.0125 | 0.0176 | 0.0149 | 0.0084 | 0.0187 | 0.0048 | 0.0162 | 0.0416 | 0.0155 | 0.004 | 0.0078 | 0.0024 | 0.0093 | 0.0293 | 0.0037 | 0.0027 |
Vacuole Periphery | 0.0048 | 0.0008 | 0.0028 | 0.0045 | 0.0058 | 0.0028 | 0.0034 | 0.0008 | 0.0025 | 0.0192 | 0.0054 | 0.0021 | 0.0015 | 0.0005 | 0.0022 | 0.0079 | 0.002 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.168 | 17.3048 | 31.8952 | 38.5136 | 25.0985 | 28.0847 | 28.503 | 40.38 | 41.3606 | 37.1828 |
Translational Efficiency | 1.4267 | 1.728 | 0.9309 | 0.9135 | 0.9748 | 1.1742 | 1.1777 | 0.6727 | 0.7514 | 0.6609 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|