Standard name
Human Ortholog
Description Hexokinase isoenzyme 2; phosphorylates glucose in cytosol; predominant hexokinase during growth on glucose; represses expression of HXK1, GLK1, induces expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at Ser14 by kinase Snf1p, phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; functions downstream of Sit4p in control of cell cycle, mitochondrial function, oxidative stress resistance, chronological lifespan; has paralog HXK1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 1.0 1.0 – 0.99 0.96 0.97 0.96 0.95 0.96 0.94 1.0 1.0 0.99 0.98 1.0 0.99 0.96 0.94 0.83 0.92 0.96 0.94
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0.11 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Bud 1 0 0 0 – 0 1 0 1 0 1 0 0 1 1 0 0 0 1 1 1 0 1 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 – 2 8 8 4 6 2 3 0 1 3 5 2 0 0 0 0 0 0 0
Cytoplasm 289 50 62 40 – 136 167 230 148 149 108 115 146 245 274 332 196 184 262 68 60 67 139 130
Endoplasmic Reticulum 0 0 0 0 – 0 3 4 4 5 1 6 0 0 1 14 22 14 1 0 0 1 0 0
Endosome 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0
Nucleus 0 0 0 0 – 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0
Unique Cell Count 291 50 62 40 137 174 237 154 157 112 122 146 245 277 338 196 186 272 73 73 73 145 139
Labelled Cell Count 292 50 62 40 138 180 242 157 162 113 124 146 247 279 354 221 199 272 73 73 73 145 139


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 248.4 251.1 213.4 251.6 – 240.6 206.1 203.1 200.6 171.4 172.6 169.0 303.3 308.8 301.2 283.7 299.6 364.6 251.9 274.6 302.3
Std Deviation (1e-4) 45.4 40.5 100.0 80.4 – 65.7 63.3 64.9 71.1 55.8 69.1 50.6 53.8 62.4 60.5 106.5 104.7 117.0 68.8 55.7 85.8
Intensity Change (Log2) – – – 0.24 – 0.17 -0.05 -0.07 -0.09 -0.32 -0.31 -0.34 0.51 0.53 0.5 0.41 0.49 0.77 0.24 0.36 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 – -0.7 -1.6 -1.4 -1.6 -1.8 -1.5 -1.9 0 0 -0.8 -1.1 0 -0.8
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 2.8 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 212.8998 280.5663 257.5899 239.0329 216.8561 246.1902 193.8577 251.1899 228.4774 197.8829 190.8985 163.9085 208.2498 271.7524 244.2747 227.8459 188.0332 231.8168
Actin 0.0059 0.0001 0.0001 0 0.0055 0.0002 0.0025 0.0001 0.003 0.0005 0.0027 0.0006 0.0082 0.0001 0.008 0.0007 0.024 0.0001
Bud 0.0024 0 0.0015 0.0001 0.0017 0.0002 0.0005 0 0.0011 0.0001 0.001 0.0002 0.0004 0 0.0005 0.0006 0.0008 0.0001
Bud Neck 0.0006 0.0002 0.0003 0.0004 0.0009 0.0018 0.0003 0.0001 0.0003 0.0003 0.0006 0.0008 0.002 0.0001 0.0002 0.0002 0.0006 0.0002
Bud Periphery 0.0013 0 0.0006 0 0.0015 0 0.0005 0 0.0006 0.0001 0.0006 0 0.0005 0 0.0009 0.0003 0.0013 0
Bud Site 0.0012 0 0.0002 0.0001 0.0004 0 0.0003 0 0.0011 0.0001 0.0021 0.0001 0.0014 0 0.0006 0.0002 0.0041 0
Cell Periphery 0.0021 0.0001 0.0004 0.0031 0.0047 0.0001 0.0004 0 0.0008 0.0001 0.0013 0.0001 0.0012 0 0.0004 0.0002 0.0012 0
Cytoplasm 0.9167 0.9975 0.9639 0.954 0.8459 0.9912 0.9322 0.9989 0.9648 0.9457 0.8746 0.9531 0.881 0.9941 0.9171 0.934 0.7696 0.7969
Cytoplasmic Foci 0.0076 0 0.0046 0.0143 0.0041 0.0003 0.0048 0 0.001 0.0003 0.0048 0.0018 0.0298 0.0001 0.011 0.0042 0.0044 0.1416
Eisosomes 0.0006 0 0.0001 0.0002 0.0001 0 0.0002 0 0.002 0.0001 0.0023 0 0.0006 0 0.0006 0.0001 0.0009 0
Endoplasmic Reticulum 0.0009 0.0001 0.0002 0.0003 0.0025 0.0003 0.0016 0 0.0008 0.0009 0.003 0.001 0.0015 0.0006 0.0003 0.001 0.0006 0.0001
Endosome 0.0026 0 0.001 0.0001 0.0434 0.0002 0.0013 0 0.0009 0.0001 0.006 0.0019 0.0027 0 0.0047 0.0003 0.0091 0.0004
Golgi 0.0012 0 0.0005 0 0.0218 0 0.0002 0 0.0004 0.0001 0.0006 0 0.0012 0 0.0042 0.0001 0.0059 0.0003
Lipid Particles 0.0019 0 0.0043 0.0026 0.0047 0 0.0003 0 0.0007 0 0.0022 0 0.0057 0 0.0044 0.0006 0.0073 0.0266
Mitochondria 0.0074 0 0.0033 0.0027 0.0143 0 0.0025 0 0.0061 0.0455 0.0516 0.0004 0.0077 0 0.0162 0.0165 0.121 0.0019
None 0.006 0.0001 0.0047 0.002 0.0064 0.0001 0.0078 0.0001 0.0015 0.0008 0.0136 0.0016 0.0105 0.0034 0.0059 0.0061 0.0039 0.0023
Nuclear Periphery 0.0051 0.0004 0.001 0.0015 0.0089 0.0015 0.0213 0.0001 0.0019 0.001 0.0073 0.0093 0.0062 0.0003 0.001 0.0042 0.0023 0.0005
Nucleolus 0.0097 0 0.0041 0.0075 0.0018 0 0.0035 0 0.0014 0.0001 0.0035 0.0002 0.014 0 0.0114 0.0074 0.0178 0.0064
Nucleus 0.0118 0.0011 0.0054 0.0046 0.0098 0.0024 0.0126 0.0006 0.0036 0.0026 0.005 0.0259 0.0088 0.001 0.0035 0.017 0.0053 0.0019
Peroxisomes 0.0017 0 0.0007 0.0003 0.0006 0 0.0001 0 0.0002 0.0001 0.0006 0 0.0069 0 0.0047 0.0006 0.0067 0.0016
Punctate Nuclear 0.0025 0 0.0004 0.0002 0.0005 0 0.0015 0 0.0003 0.0001 0.0006 0.0011 0.0023 0 0.0013 0.0009 0.0004 0.0175
Vacuole 0.0075 0.0004 0.0021 0.0055 0.0149 0.0014 0.0028 0 0.0042 0.0005 0.0088 0.0013 0.0039 0.0001 0.0013 0.0021 0.0051 0.0007
Vacuole Periphery 0.0033 0 0.0007 0.0006 0.0056 0.0003 0.0029 0 0.0034 0.0009 0.0072 0.0006 0.0036 0 0.0017 0.0027 0.0077 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1179.306 998.5287 894.7526 920.5084 981.159 1299.8019 1408.4385 1287.0491 1236.8775 1255.4012
Translational Efficiency 1.3529 1.5353 1.4036 1.5318 1.4397 1.5087 1.571 1.4082 1.5082 1.4768

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Hexokinase isoenzyme 2; phosphorylates glucose in cytosol; predominant hexokinase during growth on glucose; represses expression of HXK1, GLK1, induces expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at Ser14 by kinase Snf1p, phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; functions downstream of Sit4p in control of cell cycle, mitochondrial function, oxidative stress resistance, chronological lifespan; has paralog HXK1
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Hxk2

Hxk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hxk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available