Standard name | |
---|---|
Human Ortholog | |
Description | High-affinity zinc transporter of the plasma membrane; responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 22 | 11 | 13 | 1 | 3 | 7 | 2 | 3 | 4 |
Bud | 0 | 0 | 1 | 1 | – | 15 | – | 15 | 25 | 16 | 29 | – | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 7 | 4 | 4 | 10 | 13 |
Bud Neck | 1 | 4 | 3 | 2 | – | 15 | – | 4 | 1 | 12 | 4 | – | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | – | 1 | 5 | 10 | 5 | – | 0 | 0 | 0 | 2 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 3 | – | 0 | – | 2 | 2 | 2 | 3 | – | 4 | 2 | 0 | 15 | 15 | 22 | 0 | 3 | 3 | 2 | 1 | 2 |
Cytoplasm | 164 | 375 | 87 | 433 | – | 600 | – | 636 | 582 | 660 | 473 | – | 333 | 419 | 356 | 118 | 61 | 93 | 146 | 315 | 285 | 200 | 218 | 218 |
Endoplasmic Reticulum | 3 | 2 | 2 | 1 | – | 0 | – | 0 | 0 | 1 | 0 | – | 12 | 5 | 4 | 2 | 3 | 8 | 2 | 4 | 3 | 7 | 10 | 7 |
Endosome | 0 | 1 | 0 | 0 | – | 0 | – | 1 | 2 | 0 | 0 | – | 1 | 1 | 1 | 6 | 3 | 4 | 1 | 3 | 4 | 0 | 4 | 2 |
Golgi | 0 | 1 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 3 | 5 | 7 | 0 | 0 | 1 | 2 | 1 | 2 |
Mitochondria | 7 | 7 | 5 | 23 | – | 215 | – | 296 | 371 | 305 | 336 | – | 0 | 0 | 0 | 13 | 7 | 9 | 8 | 21 | 32 | 32 | 35 | 40 |
Nucleus | 1 | 0 | 0 | 0 | – | 2 | – | 8 | 3 | 1 | 1 | – | 2 | 0 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 5 | – | 1 | 0 | 5 | 1 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | – | 1 | 3 | 2 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | – | 1 | 1 | 1 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 4 | – | 5 | – | 17 | 18 | 31 | 15 | – | 0 | 0 | 1 | 4 | 1 | 3 | 1 | 6 | 5 | 1 | 10 | 3 |
Vac/Vac Membrane | 0 | 3 | 0 | 2 | – | 1 | – | 6 | 11 | 9 | 2 | – | 3 | 2 | 2 | 17 | 12 | 23 | 2 | 11 | 2 | 12 | 13 | 12 |
Unique Cell Count | 171 | 383 | 90 | 445 | 690 | 825 | 800 | 864 | 663 | 347 | 427 | 364 | 182 | 105 | 161 | 172 | 393 | 366 | 280 | 321 | 318 | |||
Labelled Cell Count | 177 | 393 | 98 | 469 | 858 | 989 | 1025 | 1055 | 872 | 357 | 432 | 367 | 207 | 121 | 185 | 172 | 393 | 366 | 280 | 321 | 318 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 3.9 | 2.9 | 3.1 | – | 2.9 | – | 2.8 | 2.5 | 2.4 | 2.4 | – | 5.2 | 4.7 | 5.1 | 10.1 | 11.2 | 12.0 | 5.3 | 5.3 | 5.4 |
Std Deviation (1e-4) | 0.9 | 0.9 | 0.4 | 1.7 | – | 1.2 | – | 1.3 | 1.0 | 0.8 | 0.9 | – | 1.7 | 1.1 | 1.6 | 4.9 | 5.6 | 6.5 | 1.7 | 1.9 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.12 | – | 0.01 | – | -0.07 | -0.22 | -0.26 | -0.3 | – | 0.84 | 0.68 | 0.81 | 1.8 | 1.95 | 2.05 | 0.86 | 0.87 | 0.9 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.0 | 3.3 | 3.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3161 | 1.303 | 2.1086 | 1.2099 | 0.9701 | 0.8931 | 1.1564 | 1.2913 | 1.5474 | 1.6938 | 2.0263 | 1.228 | -1.0208 | -0.885 | -0.9641 | -1.2126 | -0.9086 | -0.9704 |
Actin | 0.0135 | 0.0033 | 0.0077 | 0.0034 | 0.0055 | 0.0074 | 0.0379 | 0.0008 | 0.0011 | 0.0004 | 0.0143 | 0.0085 | 0.0061 | 0.0004 | 0.012 | 0.0005 | 0.015 | 0.0015 |
Bud | 0.0128 | 0.0222 | 0.0166 | 0.0234 | 0.0063 | 0.0096 | 0.0006 | 0.0007 | 0.0017 | 0.0003 | 0.0005 | 0.001 | 0.0015 | 0.0027 | 0.0034 | 0 | 0.0008 | 0.0003 |
Bud Neck | 0.0072 | 0.0008 | 0.0046 | 0.002 | 0.0017 | 0.0037 | 0.0031 | 0.0002 | 0.0002 | 0.0008 | 0.0006 | 0.0007 | 0.0007 | 0.0002 | 0.0005 | 0.0001 | 0.0058 | 0.0009 |
Bud Periphery | 0.0161 | 0.0237 | 0.0231 | 0.038 | 0.0052 | 0.012 | 0.0016 | 0.0024 | 0.0048 | 0.0003 | 0.0023 | 0.0034 | 0.0058 | 0.0035 | 0.0074 | 0.0001 | 0.0015 | 0.0028 |
Bud Site | 0.0404 | 0.0342 | 0.0084 | 0.0196 | 0.0019 | 0.001 | 0.0086 | 0.0013 | 0.001 | 0.0023 | 0.0007 | 0.0004 | 0.0024 | 0.006 | 0.0048 | 0.0001 | 0.002 | 0.0001 |
Cell Periphery | 0.0039 | 0.0032 | 0.0026 | 0.0014 | 0.0006 | 0.0005 | 0.0013 | 0.0021 | 0.0012 | 0.0002 | 0.0009 | 0.0007 | 0.0013 | 0.001 | 0.0011 | 0.0001 | 0.0002 | 0.0003 |
Cytoplasm | 0.0686 | 0.1187 | 0.0764 | 0.0688 | 0.1292 | 0.1148 | 0.0338 | 0.0455 | 0.0791 | 0.09 | 0.0748 | 0.0732 | 0.0393 | 0.0898 | 0.0835 | 0.0667 | 0.0333 | 0.1102 |
Cytoplasmic Foci | 0.0384 | 0.0098 | 0.0172 | 0.0304 | 0.0156 | 0.0266 | 0.0134 | 0.0025 | 0.0078 | 0.0024 | 0.0341 | 0.0075 | 0.007 | 0.0024 | 0.0056 | 0.0048 | 0.0066 | 0.0051 |
Eisosomes | 0.0007 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0011 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0003 | 0.0004 | 0.0002 | 0.0006 | 0.0001 |
Endoplasmic Reticulum | 0.1101 | 0.1388 | 0.2194 | 0.123 | 0.0706 | 0.1379 | 0.063 | 0.0585 | 0.1027 | 0.1699 | 0.1111 | 0.0817 | 0.0693 | 0.0368 | 0.0776 | 0.0057 | 0.007 | 0.052 |
Endosome | 0.0424 | 0.0045 | 0.0146 | 0.0386 | 0.0287 | 0.0174 | 0.0139 | 0.0026 | 0.004 | 0.0014 | 0.0203 | 0.0025 | 0.004 | 0.0007 | 0.0068 | 0.003 | 0.0126 | 0.0048 |
Golgi | 0.0125 | 0.0005 | 0.0024 | 0.0212 | 0.0123 | 0.0061 | 0.004 | 0.0002 | 0.0003 | 0.0001 | 0.0044 | 0.001 | 0.0005 | 0.0001 | 0.0021 | 0.0002 | 0.0024 | 0.0004 |
Lipid Particles | 0.0231 | 0.0012 | 0.0037 | 0.012 | 0.022 | 0.0057 | 0.0141 | 0.009 | 0.0026 | 0.0003 | 0.0277 | 0.0033 | 0.0018 | 0.0003 | 0.0019 | 0.0007 | 0.0083 | 0.0015 |
Mitochondria | 0.0317 | 0.0034 | 0.0172 | 0.0279 | 0.0454 | 0.0152 | 0.0037 | 0.0004 | 0.0005 | 0.0003 | 0.0017 | 0.0013 | 0.0015 | 0.0004 | 0.0016 | 0.0004 | 0.0074 | 0.0005 |
None | 0.4742 | 0.5602 | 0.5241 | 0.4845 | 0.543 | 0.5709 | 0.7657 | 0.8475 | 0.7656 | 0.7233 | 0.6045 | 0.7951 | 0.8265 | 0.8475 | 0.7641 | 0.9124 | 0.8313 | 0.7982 |
Nuclear Periphery | 0.0294 | 0.026 | 0.0244 | 0.0203 | 0.0432 | 0.0216 | 0.009 | 0.0112 | 0.0148 | 0.0036 | 0.0102 | 0.006 | 0.0157 | 0.0037 | 0.0137 | 0.0017 | 0.0177 | 0.013 |
Nucleolus | 0.0063 | 0.0027 | 0.0012 | 0.0042 | 0.0039 | 0.0021 | 0.002 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0001 | 0.0009 | 0.0003 | 0.0004 | 0.0001 | 0.0033 | 0.0002 |
Nucleus | 0.0151 | 0.0206 | 0.0091 | 0.0218 | 0.0194 | 0.0115 | 0.0022 | 0.0028 | 0.0032 | 0.0013 | 0.0028 | 0.0021 | 0.004 | 0.0014 | 0.0042 | 0.0007 | 0.0123 | 0.0024 |
Peroxisomes | 0.021 | 0.0016 | 0.0086 | 0.0317 | 0.0072 | 0.012 | 0.0114 | 0.0001 | 0.0004 | 0.0001 | 0.0614 | 0.0057 | 0.0005 | 0.0001 | 0.0006 | 0.0004 | 0.0063 | 0.0002 |
Punctate Nuclear | 0.0078 | 0.0132 | 0.0056 | 0.0162 | 0.0219 | 0.0145 | 0.0038 | 0.0006 | 0.0018 | 0.0011 | 0.0161 | 0.0015 | 0.0057 | 0.0004 | 0.0028 | 0.0017 | 0.0226 | 0.0015 |
Vacuole | 0.0195 | 0.0096 | 0.0099 | 0.0093 | 0.0101 | 0.0076 | 0.0048 | 0.0097 | 0.0041 | 0.0012 | 0.0098 | 0.0034 | 0.0034 | 0.0016 | 0.0041 | 0.0003 | 0.002 | 0.0031 |
Vacuole Periphery | 0.0057 | 0.0017 | 0.0027 | 0.002 | 0.0056 | 0.0018 | 0.001 | 0.0013 | 0.0025 | 0.0002 | 0.0012 | 0.0007 | 0.0014 | 0.0005 | 0.0015 | 0.0001 | 0.0008 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.5362 | 37.5015 | 30.4163 | 17.3107 | 8.2219 | 33.1521 | 33.2882 | 24.6992 | 20.1935 | 22.8218 |
Translational Efficiency | 0.789 | 1.1453 | 0.8693 | 1.0859 | 1.434 | 0.8942 | 0.9144 | 1.0655 | 0.8231 | 0.6456 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|