Standard name
Human Ortholog
Description Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p; PEX31 has a paralog, PEX30, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.18 0.3 0.05 0.09 0.17 0.13 0.11 0.14
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.24 0.41 0.28 0 0 0 0 0.06 0.08
Cytoplasm 0.67 0.75 0.74 0.69 0.8 0.66 0.58 0.65 0.45 0.59 0.35 0.44 0.86 0.92 0.95 0.42 0.35 0.32 0.19 0.28 0.14 0.23 0.3 0.21
Endoplasmic Reticulum 0.05 0.15 0.08 0.11 0.06 0.05 0 0 0 0 0 0 0.1 0.05 0 0.07 0.08 0 0.34 0.27 0.23 0.32 0.26 0.26
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.12 0.2 0.26 0.12 0.45 0.55 0.41 0.69 0.59 0.78 0.73 0 0 0 0.11 0.05 0.08 0.26 0.16 0.33 0.18 0.16 0.16
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.14 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 2 1 0 0 0 0 2 0 0 0 5 6 0 27 13 15 10 20 21 33 44 52
Bud 2 3 3 2 2 5 7 8 8 5 13 2 1 2 0 8 1 1 3 2 1 2 4 12
Bud Neck 0 1 0 0 0 0 0 1 0 0 0 1 1 0 0 0 1 0 3 6 1 4 8 11
Bud Site 0 0 0 0 0 0 1 1 0 2 2 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 7 0 8 0 6 5 1 1 2 6 6 8 9 21 36 30 14 1 4 3 10 23 31
Cytoplasm 100 156 76 87 88 130 156 221 80 134 77 137 247 557 498 63 26 16 38 67 17 58 119 80
Endoplasmic Reticulum 8 31 8 14 7 9 6 0 4 4 5 3 28 32 14 11 6 1 70 63 29 82 105 100
Endosome 7 0 0 0 1 1 2 6 0 0 0 0 1 2 3 12 1 1 7 7 1 3 4 8
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 3 2 1 3 0 2
Mitochondria 37 25 21 33 13 89 150 140 124 134 175 229 4 12 6 16 4 4 53 38 43 44 65 61
Nucleus 0 1 2 1 4 2 1 1 0 2 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Nuclear Periphery 1 1 0 0 0 1 1 2 0 1 0 2 0 0 0 0 0 0 1 1 0 0 1 1
Nucleolus 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 1 1 2 0 0 1 0 0 0 0 0 0 1 5 0 1 0 0 0 1 2 1
SpindlePole 0 0 0 0 0 0 3 10 1 1 5 7 1 0 0 5 1 2 1 1 0 0 2 3
Vac/Vac Membrane 7 4 0 0 3 3 3 7 2 3 1 5 2 4 2 13 1 7 8 16 3 5 10 13
Unique Cell Count 149 209 103 126 110 196 271 338 179 227 223 314 287 606 523 149 74 50 209 238 129 255 401 390
Labelled Cell Count 169 232 113 147 120 247 335 399 222 288 284 395 298 624 546 196 86 62 209 238 129 255 401 390


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.5 4.3 4.2 4.1 3.7 3.0 3.5 3.3 2.7 2.8 2.6 4.7 4.6 4.8 6.9 8.0 8.6 4.7 4.9 5.7
Std Deviation (1e-4) 0.8 1.1 1.3 1.7 1.3 1.5 1.1 1.5 1.5 0.9 0.8 1.0 1.1 1.3 1.3 1.2 1.4 1.3 1.4 1.5 2.2
Intensity Change (Log2) – – – -0.02 -0.05 -0.2 -0.5 -0.27 -0.37 -0.64 -0.59 -0.73 0.14 0.1 0.17 0.69 0.9 1.0 0.13 0.19 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 3.1 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3819 0.8512 0.5942 0.4512 0.0817 0.6804 0.8619 0.7154 0.9316 0.406 0.1671 0.7774 2.4464 2.2705 1.9278 2.3503 2.1021 1.8958
Actin 0.0232 0.0014 0.0035 0.0015 0.0037 0.0034 0.0298 0.0051 0.0115 0.0016 0.0025 0.0129 0.0538 0.001 0.0138 0.0098 0.0593 0.0101
Bud 0.0084 0.0002 0.0009 0.0001 0.0002 0.0001 0.0006 0.0009 0.0016 0.001 0.0001 0.0005 0.0011 0.0028 0.0071 0.0008 0.0003 0.0002
Bud Neck 0.0014 0.0003 0.0006 0.0004 0.0008 0.0031 0.0021 0.0007 0.002 0.001 0.0019 0.0063 0.0054 0.0003 0.0067 0.0017 0.0018 0.0036
Bud Periphery 0.0016 0.0004 0.0005 0.0005 0.0009 0.0004 0.0016 0.0015 0.0023 0.0031 0.0003 0.0015 0.0039 0.0028 0.0054 0.0018 0.0005 0.0009
Bud Site 0.0081 0.0086 0.0061 0.0001 0.0008 0.0005 0.0108 0.0187 0.0071 0.0003 0.0009 0.0007 0.0059 0.0216 0.0035 0.0032 0.0009 0.0002
Cell Periphery 0.0022 0.0016 0.0013 0.0005 0.0012 0.0016 0.0035 0.002 0.002 0.0015 0.001 0.0005 0.0027 0.003 0.0022 0.0005 0.0008 0.0005
Cytoplasm 0.136 0.2757 0.2241 0.2729 0.2428 0.3264 0.1289 0.1348 0.1454 0.157 0.1945 0.2082 0.1389 0.2044 0.2217 0.1432 0.2421 0.2345
Cytoplasmic Foci 0.015 0.0089 0.0142 0.07 0.0107 0.0285 0.0407 0.0137 0.0175 0.005 0.0113 0.016 0.0273 0.0222 0.0105 0.0292 0.0156 0.0119
Eisosomes 0.0021 0.0003 0.0005 0.0004 0.0007 0.0003 0.0013 0.0007 0.0009 0.0005 0.0003 0.0003 0.0013 0.0004 0.0005 0.0004 0.0007 0.0003
Endoplasmic Reticulum 0.0324 0.0347 0.0171 0.0588 0.0336 0.0135 0.0164 0.0112 0.0233 0.0059 0.0043 0.0175 0.0217 0.0102 0.0217 0.0303 0.0084 0.0106
Endosome 0.0042 0.0039 0.0038 0.0127 0.014 0.0064 0.0231 0.0054 0.0135 0.0015 0.0018 0.0151 0.0096 0.0019 0.0049 0.0133 0.0064 0.0025
Golgi 0.002 0.0005 0.0012 0.001 0.0007 0.0007 0.0134 0.004 0.0052 0.0003 0.0014 0.0041 0.0042 0.0003 0.0017 0.0038 0.0031 0.001
Lipid Particles 0.0111 0.0021 0.0042 0.0059 0.0019 0.0018 0.0207 0.0082 0.0092 0.0011 0.0114 0.0099 0.0168 0.0026 0.0043 0.0155 0.0044 0.0074
Mitochondria 0.0008 0.0002 0.0006 0.0008 0.0004 0.0009 0.0025 0.0015 0.0132 0.0007 0.0005 0.0017 0.0022 0.0021 0.0047 0.0086 0.0009 0.0006
None 0.7449 0.6559 0.7114 0.5559 0.6789 0.6084 0.6617 0.7827 0.7209 0.815 0.7416 0.6847 0.675 0.7169 0.6762 0.6992 0.6442 0.7077
Nuclear Periphery 0.0018 0.0009 0.0013 0.0084 0.0028 0.0006 0.0048 0.0028 0.0033 0.0007 0.0003 0.0045 0.0063 0.0007 0.0021 0.0031 0.004 0.0006
Nucleolus 0.0003 0.0002 0.0008 0.0001 0.0002 0.0001 0.001 0.0004 0.0005 0.0003 0.0005 0.0002 0.0006 0.0008 0.0004 0.0015 0.0002 0.0001
Nucleus 0.0007 0.001 0.0018 0.0016 0.0021 0.0007 0.0017 0.0007 0.0013 0.0007 0.0006 0.001 0.0022 0.0011 0.0023 0.0045 0.0007 0.0005
Peroxisomes 0.0011 0.0001 0.0003 0.0009 0.0002 0.001 0.0188 0.0016 0.0119 0.0004 0.0226 0.0099 0.0106 0.0008 0.007 0.0107 0.0028 0.0053
Punctate Nuclear 0.0011 0.001 0.0036 0.0034 0.0018 0.0008 0.0133 0.002 0.0042 0.0008 0.0014 0.0029 0.0072 0.0019 0.0015 0.0164 0.0019 0.0009
Vacuole 0.0012 0.0018 0.0019 0.0032 0.0016 0.0007 0.0028 0.0013 0.0029 0.0012 0.0007 0.0013 0.0025 0.0022 0.0014 0.0019 0.0008 0.0005
Vacuole Periphery 0.0002 0.0001 0.0002 0.0009 0.0002 0.0002 0.0007 0.0003 0.0005 0.0002 0.0001 0.0004 0.0006 0.0001 0.0003 0.0005 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7102 7.8115 5.2695 11.3119 7.2097 7.1455 7.8982 8.723 11.5098 13.0502
Translational Efficiency 0.7544 0.9007 1.5937 0.6552 1.2249 1.2688 0.9381 0.6503 0.6965 0.7006

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p; PEX31 has a paralog, PEX30, that arose from the whole genome duplication
Localization
Cell Percentages ER (50%), cytoplasm (12%), mixed (35%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pex31

Pex31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available