Standard name
Human Ortholog
Description Ethanolamine-phosphate cytidylyltransferase; catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases; inability of the null mutant to synthesize phosphatidylethanolamine and phosphatidylcholine from ethanolamine is functionally complemented by human PCYT2

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.97 0.92 0.85 0.78 0.8 0.79 0.72 0.67 0.72 0.99 0.98 0.97 0.94 0.93 0.93 0.97 0.96 0.95 0.97 0.93 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.13 0.1 0.16 0.17 0.35 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0.12 0.14 0.09 0.08 0.16 0.05 0.13 0 0 0 0.08 0.08 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Bud 0 0 0 0 0 0 3 5 0 12 8 8 0 0 0 0 1 0 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 1 2 3 0 4 3 4 2 3 5 8 8 1 1 2 10 10 15 0 0 0 0 0 0
Cytoplasm 743 376 352 335 533 429 391 397 387 407 262 349 255 366 387 301 315 309 713 352 339 187 315 244
Endoplasmic Reticulum 2 1 1 1 1 1 5 4 6 0 2 3 0 1 4 7 8 6 0 2 3 0 1 2
Endosome 0 0 0 0 0 0 0 1 0 0 1 1 0 1 0 3 1 1 2 1 2 2 2 0
Golgi 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 1 0
Mitochondria 4 1 0 1 7 32 66 48 80 94 138 83 0 0 0 3 3 6 4 1 3 0 3 2
Nucleus 4 1 1 1 3 14 14 18 10 20 9 20 1 4 3 3 2 3 2 4 0 1 1 0
Nuclear Periphery 0 0 0 0 0 2 0 2 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1
Vac/Vac Membrane 5 3 4 14 54 58 68 45 40 93 18 62 3 14 16 25 28 16 1 2 1 0 4 2
Unique Cell Count 751 381 358 345 581 503 500 496 490 567 391 485 258 372 398 320 337 332 738 369 356 194 338 257
Labelled Cell Count 759 384 363 352 604 539 551 523 527 632 449 534 261 387 412 352 371 359 738 369 356 194 338 257


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 11.0 8.6 8.3 8.4 7.1 6.5 6.9 5.9 6.1 5.4 5.9 9.1 9.2 8.9 11.1 12.2 11.7 10.2 10.3 11.6
Std Deviation (1e-4) 1.1 1.5 1.2 1.0 1.2 1.2 1.1 1.0 1.0 0.8 1.0 0.9 1.5 1.0 1.1 2.0 2.0 2.1 1.4 1.5 1.8
Intensity Change (Log2) -0.05 -0.03 -0.29 -0.4 -0.32 -0.54 -0.51 -0.68 -0.55 0.08 0.09 0.04 0.37 0.5 0.44 0.24 0.26 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1.4 0 0 0 0 2.2 2.1 3.0
Cytoplasm -1.1 -4.2 -6.5 -8.5 -8.0 -8.3 -10.2 -11.1 -10.1 0.5 0.1 -1.0 -2.9 -3.3 -3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 7.2 6.1 8.0 8.1 12.4 8.2 0 0 0 0 0 0
Nucleus 0 0 2.8 2.8 3.3 0 3.2 0 3.5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.5 5.1 5.8 6.5 4.9 4.6 7.4 2.8 6.2 0 2.3 2.5 4.3 4.5 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2798 3.6176 3.3167 3.1634 2.5646 3.1738 4.1094 5.4237 4.9905 4.5781 4.8187 5.0176 4.846 5.8037 5.038 4.1924 5.2976 5.2047
Actin 0.0292 0.0007 0.0052 0.0101 0.0551 0.0043 0.0005 0.0013 0.002 0.0059 0.0006 0.001 0.0376 0.0011 0.0082 0.0061 0.0002 0.002
Bud 0.0014 0.0004 0.0008 0.0009 0.0007 0.0006 0.0019 0.0058 0.0027 0.0212 0.0096 0.0031 0.0016 0.0101 0.0129 0.0055 0.0148 0.009
Bud Neck 0.0088 0.0005 0.0063 0.0017 0.0013 0.0023 0.0009 0.0006 0.0008 0.0014 0.001 0.0069 0.0038 0.0004 0.0054 0.0354 0.0008 0.0023
Bud Periphery 0.0017 0.0001 0.001 0.0007 0.0006 0.0005 0.0016 0.0016 0.0028 0.0044 0.0019 0.0008 0.0013 0.0016 0.0042 0.0013 0.0018 0.0029
Bud Site 0.0052 0.0047 0.0032 0.0024 0.0016 0.0003 0.0009 0.0084 0.0011 0.0026 0.0018 0.0003 0.013 0.0072 0.0114 0.0021 0.0002 0.0015
Cell Periphery 0.0008 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0003 0.0005 0.0002 0.0002 0.0001 0.0003
Cytoplasm 0.6292 0.8932 0.8421 0.817 0.6803 0.8398 0.8578 0.8955 0.8702 0.8776 0.8951 0.8507 0.7802 0.9113 0.8678 0.8076 0.913 0.8351
Cytoplasmic Foci 0.0434 0.012 0.0217 0.0185 0.0358 0.015 0.0109 0.008 0.0067 0.0089 0.0108 0.0116 0.0263 0.0108 0.0117 0.0127 0.0098 0.0135
Eisosomes 0.0003 0 0 0.0001 0.0002 0.0001 0 0 0 0 0 0 0.0003 0 0 0.0001 0 0.0001
Endoplasmic Reticulum 0.0081 0.0026 0.0044 0.0039 0.0042 0.0045 0.003 0.002 0.0024 0.0027 0.0031 0.0028 0.0054 0.0023 0.0021 0.0017 0.0012 0.002
Endosome 0.0454 0.0043 0.0086 0.012 0.029 0.0186 0.0036 0.0032 0.0032 0.0032 0.0047 0.005 0.0262 0.0046 0.0036 0.0056 0.0025 0.0125
Golgi 0.0153 0.0003 0.0016 0.0061 0.0086 0.0024 0.0002 0.0002 0.0002 0.0003 0.0002 0.0002 0.0079 0.0011 0.0014 0.0022 0.0001 0.006
Lipid Particles 0.0113 0.0001 0.0006 0.0008 0.0164 0.0023 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0089 0.0003 0.001 0.008 0 0.0011
Mitochondria 0.0103 0.0003 0.001 0.005 0.0029 0.0041 0.0004 0.0004 0.0005 0.0015 0.0013 0.0003 0.0057 0.0032 0.0015 0.0029 0.0005 0.0099
None 0.0995 0.0557 0.0621 0.0527 0.0877 0.0354 0.0689 0.039 0.0572 0.0128 0.0242 0.0474 0.0213 0.017 0.0202 0.0107 0.0109 0.0244
Nuclear Periphery 0.0224 0.0027 0.0042 0.0123 0.006 0.0145 0.0035 0.0023 0.0032 0.0028 0.0039 0.0034 0.0072 0.0025 0.0038 0.0042 0.0022 0.0039
Nucleolus 0.0011 0.0001 0.0018 0.0007 0.0003 0.0023 0.0002 0.0002 0.0002 0.0011 0.0006 0.0003 0.0004 0.0009 0.0004 0.0023 0.0005 0.0096
Nucleus 0.0299 0.0165 0.0248 0.0291 0.0224 0.0375 0.0386 0.0251 0.0407 0.0455 0.0341 0.0513 0.0314 0.0185 0.0318 0.0612 0.0347 0.0498
Peroxisomes 0.0154 0.0004 0.0024 0.0093 0.0333 0.0011 0.0014 0.0003 0.0005 0.0014 0.0002 0.0003 0.0117 0.0012 0.0056 0.0181 0.0002 0.0013
Punctate Nuclear 0.014 0.0031 0.0058 0.0147 0.0119 0.0095 0.0042 0.0047 0.0044 0.0047 0.0044 0.0133 0.0067 0.0034 0.0052 0.0103 0.0054 0.0101
Vacuole 0.0052 0.0019 0.0018 0.0015 0.0012 0.0029 0.0011 0.001 0.0009 0.0013 0.002 0.001 0.0019 0.0016 0.0011 0.0013 0.0009 0.0021
Vacuole Periphery 0.002 0.0002 0.0004 0.0005 0.0005 0.002 0.0002 0.0001 0.0002 0.0004 0.0003 0.0002 0.0007 0.0003 0.0003 0.0004 0.0002 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.4076 48.7099 48.1932 42.3755 31.6442 29.4986 53.6113 47.0911 50.2469 48.3422
Translational Efficiency 0.7748 0.8936 0.6094 0.7655 0.984 1.0391 0.7886 0.6652 0.6 0.7251

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ethanolamine-phosphate cytidylyltransferase; catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases; inability of the null mutant to synthesize phosphatidylethanolamine and phosphatidylcholine from ethanolamine is functionally complemented by human PCYT2
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ect1

Ect1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ect1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available