Standard name | |
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Human Ortholog | |
Description | Ethanolamine-phosphate cytidylyltransferase; catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases; inability of the null mutant to synthesize phosphatidylethanolamine and phosphatidylcholine from ethanolamine is functionally complemented by human PCYT2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 0 | 12 | 8 | 8 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 3 | 0 | 4 | 3 | 4 | 2 | 3 | 5 | 8 | 8 | 1 | 1 | 2 | 10 | 10 | 15 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 743 | 376 | 352 | 335 | 533 | 429 | 391 | 397 | 387 | 407 | 262 | 349 | 255 | 366 | 387 | 301 | 315 | 309 | 713 | 352 | 339 | 187 | 315 | 244 |
Endoplasmic Reticulum | 2 | 1 | 1 | 1 | 1 | 1 | 5 | 4 | 6 | 0 | 2 | 3 | 0 | 1 | 4 | 7 | 8 | 6 | 0 | 2 | 3 | 0 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 1 | 1 | 2 | 1 | 2 | 2 | 2 | 0 |
Golgi | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 4 | 1 | 0 | 1 | 7 | 32 | 66 | 48 | 80 | 94 | 138 | 83 | 0 | 0 | 0 | 3 | 3 | 6 | 4 | 1 | 3 | 0 | 3 | 2 |
Nucleus | 4 | 1 | 1 | 1 | 3 | 14 | 14 | 18 | 10 | 20 | 9 | 20 | 1 | 4 | 3 | 3 | 2 | 3 | 2 | 4 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 5 | 3 | 4 | 14 | 54 | 58 | 68 | 45 | 40 | 93 | 18 | 62 | 3 | 14 | 16 | 25 | 28 | 16 | 1 | 2 | 1 | 0 | 4 | 2 |
Unique Cell Count | 751 | 381 | 358 | 345 | 581 | 503 | 500 | 496 | 490 | 567 | 391 | 485 | 258 | 372 | 398 | 320 | 337 | 332 | 738 | 369 | 356 | 194 | 338 | 257 |
Labelled Cell Count | 759 | 384 | 363 | 352 | 604 | 539 | 551 | 523 | 527 | 632 | 449 | 534 | 261 | 387 | 412 | 352 | 371 | 359 | 738 | 369 | 356 | 194 | 338 | 257 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.1 | 11.0 | 8.6 | 8.3 | 8.4 | 7.1 | 6.5 | 6.9 | 5.9 | 6.1 | 5.4 | 5.9 | 9.1 | 9.2 | 8.9 | 11.1 | 12.2 | 11.7 | 10.2 | 10.3 | 11.6 |
Std Deviation (1e-4) | 1.1 | 1.5 | 1.2 | 1.0 | 1.2 | 1.2 | 1.1 | 1.0 | 1.0 | 0.8 | 1.0 | 0.9 | 1.5 | 1.0 | 1.1 | 2.0 | 2.0 | 2.1 | 1.4 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.03 | -0.29 | -0.4 | -0.32 | -0.54 | -0.51 | -0.68 | -0.55 | 0.08 | 0.09 | 0.04 | 0.37 | 0.5 | 0.44 | 0.24 | 0.26 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 | 0 | 0 | 2.2 | 2.1 | 3.0 |
Cytoplasm | -1.1 | -4.2 | -6.5 | -8.5 | -8.0 | -8.3 | -10.2 | -11.1 | -10.1 | 0.5 | 0.1 | -1.0 | -2.9 | -3.3 | -3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.9 | 7.2 | 6.1 | 8.0 | 8.1 | 12.4 | 8.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 2.8 | 2.8 | 3.3 | 0 | 3.2 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.5 | 5.1 | 5.8 | 6.5 | 4.9 | 4.6 | 7.4 | 2.8 | 6.2 | 0 | 2.3 | 2.5 | 4.3 | 4.5 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2798 | 3.6176 | 3.3167 | 3.1634 | 2.5646 | 3.1738 | 4.1094 | 5.4237 | 4.9905 | 4.5781 | 4.8187 | 5.0176 | 4.846 | 5.8037 | 5.038 | 4.1924 | 5.2976 | 5.2047 |
Actin | 0.0292 | 0.0007 | 0.0052 | 0.0101 | 0.0551 | 0.0043 | 0.0005 | 0.0013 | 0.002 | 0.0059 | 0.0006 | 0.001 | 0.0376 | 0.0011 | 0.0082 | 0.0061 | 0.0002 | 0.002 |
Bud | 0.0014 | 0.0004 | 0.0008 | 0.0009 | 0.0007 | 0.0006 | 0.0019 | 0.0058 | 0.0027 | 0.0212 | 0.0096 | 0.0031 | 0.0016 | 0.0101 | 0.0129 | 0.0055 | 0.0148 | 0.009 |
Bud Neck | 0.0088 | 0.0005 | 0.0063 | 0.0017 | 0.0013 | 0.0023 | 0.0009 | 0.0006 | 0.0008 | 0.0014 | 0.001 | 0.0069 | 0.0038 | 0.0004 | 0.0054 | 0.0354 | 0.0008 | 0.0023 |
Bud Periphery | 0.0017 | 0.0001 | 0.001 | 0.0007 | 0.0006 | 0.0005 | 0.0016 | 0.0016 | 0.0028 | 0.0044 | 0.0019 | 0.0008 | 0.0013 | 0.0016 | 0.0042 | 0.0013 | 0.0018 | 0.0029 |
Bud Site | 0.0052 | 0.0047 | 0.0032 | 0.0024 | 0.0016 | 0.0003 | 0.0009 | 0.0084 | 0.0011 | 0.0026 | 0.0018 | 0.0003 | 0.013 | 0.0072 | 0.0114 | 0.0021 | 0.0002 | 0.0015 |
Cell Periphery | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0003 |
Cytoplasm | 0.6292 | 0.8932 | 0.8421 | 0.817 | 0.6803 | 0.8398 | 0.8578 | 0.8955 | 0.8702 | 0.8776 | 0.8951 | 0.8507 | 0.7802 | 0.9113 | 0.8678 | 0.8076 | 0.913 | 0.8351 |
Cytoplasmic Foci | 0.0434 | 0.012 | 0.0217 | 0.0185 | 0.0358 | 0.015 | 0.0109 | 0.008 | 0.0067 | 0.0089 | 0.0108 | 0.0116 | 0.0263 | 0.0108 | 0.0117 | 0.0127 | 0.0098 | 0.0135 |
Eisosomes | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0081 | 0.0026 | 0.0044 | 0.0039 | 0.0042 | 0.0045 | 0.003 | 0.002 | 0.0024 | 0.0027 | 0.0031 | 0.0028 | 0.0054 | 0.0023 | 0.0021 | 0.0017 | 0.0012 | 0.002 |
Endosome | 0.0454 | 0.0043 | 0.0086 | 0.012 | 0.029 | 0.0186 | 0.0036 | 0.0032 | 0.0032 | 0.0032 | 0.0047 | 0.005 | 0.0262 | 0.0046 | 0.0036 | 0.0056 | 0.0025 | 0.0125 |
Golgi | 0.0153 | 0.0003 | 0.0016 | 0.0061 | 0.0086 | 0.0024 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0079 | 0.0011 | 0.0014 | 0.0022 | 0.0001 | 0.006 |
Lipid Particles | 0.0113 | 0.0001 | 0.0006 | 0.0008 | 0.0164 | 0.0023 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0089 | 0.0003 | 0.001 | 0.008 | 0 | 0.0011 |
Mitochondria | 0.0103 | 0.0003 | 0.001 | 0.005 | 0.0029 | 0.0041 | 0.0004 | 0.0004 | 0.0005 | 0.0015 | 0.0013 | 0.0003 | 0.0057 | 0.0032 | 0.0015 | 0.0029 | 0.0005 | 0.0099 |
None | 0.0995 | 0.0557 | 0.0621 | 0.0527 | 0.0877 | 0.0354 | 0.0689 | 0.039 | 0.0572 | 0.0128 | 0.0242 | 0.0474 | 0.0213 | 0.017 | 0.0202 | 0.0107 | 0.0109 | 0.0244 |
Nuclear Periphery | 0.0224 | 0.0027 | 0.0042 | 0.0123 | 0.006 | 0.0145 | 0.0035 | 0.0023 | 0.0032 | 0.0028 | 0.0039 | 0.0034 | 0.0072 | 0.0025 | 0.0038 | 0.0042 | 0.0022 | 0.0039 |
Nucleolus | 0.0011 | 0.0001 | 0.0018 | 0.0007 | 0.0003 | 0.0023 | 0.0002 | 0.0002 | 0.0002 | 0.0011 | 0.0006 | 0.0003 | 0.0004 | 0.0009 | 0.0004 | 0.0023 | 0.0005 | 0.0096 |
Nucleus | 0.0299 | 0.0165 | 0.0248 | 0.0291 | 0.0224 | 0.0375 | 0.0386 | 0.0251 | 0.0407 | 0.0455 | 0.0341 | 0.0513 | 0.0314 | 0.0185 | 0.0318 | 0.0612 | 0.0347 | 0.0498 |
Peroxisomes | 0.0154 | 0.0004 | 0.0024 | 0.0093 | 0.0333 | 0.0011 | 0.0014 | 0.0003 | 0.0005 | 0.0014 | 0.0002 | 0.0003 | 0.0117 | 0.0012 | 0.0056 | 0.0181 | 0.0002 | 0.0013 |
Punctate Nuclear | 0.014 | 0.0031 | 0.0058 | 0.0147 | 0.0119 | 0.0095 | 0.0042 | 0.0047 | 0.0044 | 0.0047 | 0.0044 | 0.0133 | 0.0067 | 0.0034 | 0.0052 | 0.0103 | 0.0054 | 0.0101 |
Vacuole | 0.0052 | 0.0019 | 0.0018 | 0.0015 | 0.0012 | 0.0029 | 0.0011 | 0.001 | 0.0009 | 0.0013 | 0.002 | 0.001 | 0.0019 | 0.0016 | 0.0011 | 0.0013 | 0.0009 | 0.0021 |
Vacuole Periphery | 0.002 | 0.0002 | 0.0004 | 0.0005 | 0.0005 | 0.002 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0007 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 45.4076 | 48.7099 | 48.1932 | 42.3755 | 31.6442 | 29.4986 | 53.6113 | 47.0911 | 50.2469 | 48.3422 |
Translational Efficiency | 0.7748 | 0.8936 | 0.6094 | 0.7655 | 0.984 | 1.0391 | 0.7886 | 0.6652 | 0.6 | 0.7251 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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