Standard name
Human Ortholog
Description Protein involved in resistance to desiccation stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to F0 sector of mitochondrial F1F0 ATPase in vitro and may modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress; STF2 has a paralog, TMA10, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.99 0.94 0.91 – 0.73 0.72 0.68 0.7 0.76 0.99 0.98 1.0 0.96 0.88 0.96 0.96 0.95 0.95 0.94 0.9 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.05 0 0 0.05 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0.06 0.06 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 – 0.18 0.18 0.22 0.12 0.08 0 0 0 0 0.12 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 – 3 0 4 3 8 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 4 0 0 1 – 4 1 3 3 2 0 0 0 0 0 0 2 4 1 1 2 7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 5 9 4 – 8 10 8 10 12 0 0 0 1 0 4 0 0 0 0 0 0
Cytoplasm 169 274 361 288 352 411 – 370 236 259 259 344 87 83 85 81 36 97 162 259 352 52 60 122
Endoplasmic Reticulum 0 0 1 1 7 5 – 27 9 7 17 24 0 0 0 1 0 5 0 0 4 0 0 1
Endosome 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1
Golgi 0 0 0 0 0 0 – 0 0 2 1 2 0 0 0 0 0 0 0 1 2 0 0 0
Mitochondria 1 0 3 11 1 6 – 12 21 21 38 49 0 0 0 2 1 3 0 0 1 0 0 0
Nucleus 0 1 0 0 0 0 – 11 3 6 6 2 0 0 0 0 1 0 0 1 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 1 2 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 2 3 4 16 32 – 91 60 82 44 35 1 2 0 2 5 4 2 0 2 0 0 2
Unique Cell Count 170 277 367 290 375 453 509 328 380 368 452 88 85 85 84 41 101 170 274 370 56 68 138
Labelled Cell Count 172 278 374 309 385 460 528 342 392 382 478 88 85 85 89 43 113 170 274 370 56 68 138


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 17.5 7.5 11.4 17.5 19.1 – 21.8 23.6 22.9 25.0 26.2 13.3 22.8 27.5 25.7 23.3 23.7 9.0 10.8 12.2
Std Deviation (1e-4) 2.1 13.4 2.1 4.4 6.1 8.1 – 8.0 10.4 9.8 11.5 11.1 5.6 12.6 11.3 19.1 15.0 12.6 1.9 3.4 5.1
Intensity Change (Log2) – – – 0.61 1.23 1.35 – 1.55 1.66 1.62 1.74 1.81 0.83 1.61 1.88 1.78 1.64 1.66 0.27 0.53 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 3.1 0 0 0 0 0 0
Cytoplasm 1.1 -3.2 -4.6 – -10.1 -10.0 -11.0 -10.4 -9.2 0.3 -0.5 1.2 -1.1 -4.0 -1.4
Endoplasmic Reticulum 0 0 0 – 4.2 0 0 3.8 4.2 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 0 0 – 1.7 4.0 3.6 5.6 5.8 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.0 4.4 – 8.0 8.0 8.9 6.2 4.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.1666 19.428 18.4648 13.0711 15.5302 17.2036 19.7198 25.4276 24.6805 20.1997 17.4063 23.3203 31.949 34.0144 32.5341 31.9329 28.5005 32.16
Actin 0.0125 0.0015 0.0116 0.0053 0.0876 0.0021 0.0253 0.0008 0.0127 0.0106 0.0019 0.0106 0.0007 0.0015 0.0006 0.0073 0.0007 0.0006
Bud 0.0009 0.004 0.0042 0.0003 0.0006 0.0004 0.0012 0.0014 0.0014 0.0008 0.0014 0.0003 0.0004 0.0038 0.0005 0.0031 0.0043 0.0004
Bud Neck 0.0006 0.0002 0.0006 0.0003 0.0016 0.0012 0.0012 0.0002 0.0005 0.0008 0.0016 0.0009 0.0002 0.0002 0.0003 0.0009 0.0009 0.0007
Bud Periphery 0.0004 0.0009 0.0015 0.0001 0.001 0.0002 0.0011 0.0005 0.0005 0.0007 0.0008 0.0002 0.0002 0.0009 0.0002 0.0012 0.0026 0.0001
Bud Site 0.0014 0.0016 0.0009 0.0008 0.0009 0.0003 0.0056 0.0034 0.0012 0.0036 0.0005 0.0003 0.0002 0.0023 0.0004 0.002 0.0011 0
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0
Cytoplasm 0.9214 0.9802 0.9344 0.8339 0.7776 0.9383 0.8337 0.9865 0.9311 0.851 0.8763 0.9477 0.9763 0.9828 0.9896 0.9666 0.9647 0.9757
Cytoplasmic Foci 0.0099 0.0013 0.0087 0.0302 0.0178 0.008 0.0199 0.0013 0.0084 0.0191 0.0092 0.0055 0.0024 0.0009 0.0008 0.0018 0.003 0.0037
Eisosomes 0.0001 0 0.0001 0.0001 0.0006 0 0.0002 0 0.0001 0.0002 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0058 0.0015 0.0035 0.0101 0.0125 0.0021 0.0056 0.0008 0.0013 0.0045 0.0049 0.003 0.0016 0.0004 0.0007 0.001 0.0024 0.003
Endosome 0.0074 0.0004 0.0049 0.0228 0.0273 0.0058 0.0266 0.0004 0.0107 0.0241 0.0328 0.0145 0.0012 0.0002 0.0002 0.0014 0.0024 0.0009
Golgi 0.0017 0.0001 0.0013 0.0074 0.0101 0.0005 0.0096 0.0001 0.0068 0.0083 0.0071 0.002 0.0001 0 0 0.0001 0.0002 0.0002
Lipid Particles 0.0014 0 0.0024 0.0442 0.0064 0.0004 0.0113 0 0.0016 0.0137 0.0006 0.0005 0.0002 0 0 0 0.0001 0
Mitochondria 0.0045 0.0001 0.0025 0.0029 0.0047 0.0005 0.0169 0.0001 0.004 0.0205 0.0109 0.0024 0.0002 0.0001 0 0.0002 0.0008 0.0001
None 0.0081 0.0018 0.0022 0.0035 0.003 0.0025 0.0036 0.0008 0.0026 0.002 0.0023 0.0006 0.0029 0.0008 0.0006 0.0016 0.0007 0.0005
Nuclear Periphery 0.0038 0.0012 0.0047 0.0035 0.0236 0.0092 0.0074 0.0006 0.0019 0.0064 0.011 0.0023 0.0021 0.0009 0.001 0.0019 0.0024 0.0022
Nucleolus 0.0003 0 0.0001 0.0002 0.0005 0.0004 0.0003 0 0.002 0.0012 0.0004 0 0 0 0 0 0.0002 0
Nucleus 0.0116 0.004 0.0074 0.0043 0.0103 0.0165 0.0188 0.0023 0.0065 0.0099 0.026 0.0047 0.0095 0.0038 0.0039 0.0064 0.0059 0.0106
Peroxisomes 0.0011 0 0.0049 0.0246 0.0014 0.0004 0.0029 0.0001 0.001 0.005 0.0002 0.0012 0.0001 0.0001 0 0.0001 0.0001 0.0001
Punctate Nuclear 0.003 0.0003 0.0028 0.0035 0.0089 0.0098 0.0036 0.0002 0.001 0.0018 0.0034 0.0005 0.0006 0.0003 0.0002 0.0006 0.0002 0.0004
Vacuole 0.0031 0.0006 0.0007 0.0016 0.0021 0.0009 0.0036 0.0003 0.0033 0.0132 0.0062 0.0019 0.0008 0.0006 0.0006 0.0031 0.0059 0.0008
Vacuole Periphery 0.0009 0.0001 0.0004 0.0005 0.0016 0.0004 0.0013 0 0.0011 0.0023 0.0023 0.0008 0.0001 0.0001 0.0001 0.0005 0.001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.7801 42.5496 22.9626 48.7881 22.441 69.1949 39.1107 44.8751 93.0088 43.7093
Translational Efficiency 3.4617 1.3046 2.3628 2.3886 4.3536 4.0838 2.5787 2.409 2.2958 2.4919

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in resistance to desiccation stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to F0 sector of mitochondrial F1F0 ATPase in vitro and may modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress; STF2 has a paralog, TMA10, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Stf2

Stf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available