Standard name
Human Ortholog
Description Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); homolog of human NMNAT; NMA2 has a paralog, NMA1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.93 0.9 0.86 0.61 0.59 0.51 0.43 0.46 0.37 0.35 0.33 0.89 0.79 0.79 0.81 0.86 0.82 0.88 0.85 0.87 0.87 0.79 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.09 0.2 0.41 0.44 0.43 0.42 0.6 0.65 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.08 0.08 0.06 0.06 0.05 0.08 0 0 0 0 0 0 0 0.06 0.05 0.06 0.09 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.05 0.15 0.15 0.29 0.19 0.19 0.25 0.23 0.23 0.19 0.28 0.14 0.41 0.43 0.27 0.22 0.21 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Bud 0 1 0 0 3 7 7 4 4 3 5 9 0 1 0 0 0 0 1 2 3 1 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 3 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 0 0 1 2 0 7 2 1 2 0 0 0 2 6 5 0 0 0 0 0 0
Cytoplasm 253 399 389 242 155 228 269 170 208 183 163 145 171 243 243 351 299 246 261 391 397 151 180 183
Endoplasmic Reticulum 0 2 0 0 0 0 2 2 8 2 1 3 0 0 0 9 11 7 0 1 4 0 0 2
Endosome 1 0 6 2 1 1 0 0 0 0 0 1 1 1 10 1 2 1 4 8 3 3 2 4
Golgi 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 0 0 0
Mitochondria 13 12 9 25 52 157 229 167 190 295 302 229 0 0 0 5 2 0 4 6 5 1 1 3
Nucleus 2 4 5 7 4 12 41 31 25 30 24 36 4 6 2 17 7 11 11 27 25 10 19 14
Nuclear Periphery 0 0 0 0 0 2 1 4 3 1 2 2 0 0 0 0 0 0 1 1 1 0 0 1
Nucleolus 0 0 0 0 2 1 2 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 3 2
Vac/Vac Membrane 15 21 65 42 74 73 97 97 105 112 88 123 26 126 132 117 75 64 4 14 8 2 7 8
Unique Cell Count 267 428 434 280 255 386 523 392 448 494 464 434 192 309 306 435 348 299 298 463 459 175 229 232
Labelled Cell Count 284 442 475 318 291 482 650 477 550 632 588 551 202 377 389 502 402 334 298 463 459 175 229 232


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.1 5.9 5.4 5.7 5.0 4.9 5.1 4.9 4.8 4.6 4.9 9.0 8.9 8.9 8.1 7.4 7.4 8.4 8.6 9.3
Std Deviation (1e-4) 0.6 0.9 0.8 0.8 1.6 1.1 1.1 1.1 0.9 0.9 0.9 0.9 1.4 1.5 1.3 1.2 1.5 1.5 1.3 1.5 1.8
Intensity Change (Log2) – – – -0.12 -0.04 -0.23 -0.25 -0.21 -0.27 -0.3 -0.35 -0.26 0.61 0.6 0.6 0.47 0.33 0.34 0.51 0.55 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -9.0 -10.1 -12.7 -14.2 -13.7 -16.4 -16.8 -17.0 -0.2 -4.1 -3.9 -3.7 -1.6 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 1.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.2 8.2 13.7 14.9 14.2 0 0 0 16.7 0 0 0 -1.1 0 0
Nucleus 0 0 2.0 4.8 4.7 3.6 3.9 3.4 5.0 0 0 0 2.6 1.0 2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.4 1.5 1.5 3.5 3.2 3.0 1.6 4.8 -0.5 7.9 8.5 4.3 2.4 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1946 4.5418 4.2963 3.9336 3.9496 4.3173 2.8109 3.6585 3.6291 2.9946 3.5453 3.3582 2.0736 2.6546 2.4757 2.5925 2.1703 2.6825
Actin 0.0048 0.0012 0.0069 0.0015 0.0008 0.0033 0.0044 0.0059 0.0057 0.0017 0.0007 0.0018 0.0009 0.0035 0.0024 0.0012 0.0019 0.0018
Bud 0.0015 0.0018 0.0036 0.0011 0.0007 0.0012 0.0006 0.0027 0.0021 0.0007 0.0003 0.0007 0.0005 0.0036 0.0013 0.001 0.0076 0.0012
Bud Neck 0.0068 0.0014 0.0019 0.0031 0.0012 0.0119 0.0045 0.0016 0.0044 0.0029 0.0036 0.0062 0.0005 0.0008 0.0073 0.0018 0.0104 0.0031
Bud Periphery 0.0015 0.0006 0.0017 0.0006 0.0008 0.0012 0.0005 0.0017 0.0018 0.0004 0.0002 0.0007 0.0005 0.002 0.0008 0.0007 0.0052 0.0013
Bud Site 0.0075 0.01 0.0091 0.0028 0.0004 0.0011 0.0016 0.0183 0.0115 0.0021 0.0004 0.0008 0.0009 0.0084 0.009 0.0009 0.0149 0.0008
Cell Periphery 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0004 0.0001
Cytoplasm 0.5287 0.6203 0.4999 0.5553 0.4884 0.5237 0.6171 0.7371 0.6552 0.6611 0.6923 0.622 0.5625 0.6805 0.6509 0.6657 0.6827 0.6371
Cytoplasmic Foci 0.0149 0.0188 0.0125 0.0102 0.0091 0.017 0.016 0.0131 0.0096 0.0186 0.0139 0.0167 0.0113 0.0154 0.0137 0.014 0.0141 0.015
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0064 0.0033 0.0041 0.0051 0.0032 0.0029 0.0052 0.0046 0.0047 0.0061 0.0051 0.0034 0.0057 0.0045 0.0048 0.0047 0.0052 0.0049
Endosome 0.0108 0.0069 0.0067 0.0086 0.0059 0.009 0.0121 0.0059 0.006 0.009 0.0086 0.007 0.0107 0.0093 0.013 0.0123 0.0094 0.0128
Golgi 0.0012 0.0005 0.001 0.0002 0.0001 0.0004 0.0014 0.0007 0.0011 0.0004 0.0003 0.0004 0.0004 0.0005 0.0007 0.0003 0.0002 0.0004
Lipid Particles 0.0012 0.0002 0.0009 0.0002 0.0001 0.0002 0.0012 0.0002 0.0013 0.0004 0.0001 0.0002 0.0003 0.0005 0.0003 0.0002 0.0002 0.0002
Mitochondria 0.0019 0.0009 0.0015 0.0013 0.0007 0.0011 0.0012 0.0009 0.0011 0.0009 0.0008 0.0009 0.0016 0.0007 0.001 0.001 0.0005 0.0011
None 0.2245 0.1273 0.2087 0.0943 0.263 0.1505 0.1418 0.0816 0.132 0.0631 0.1034 0.1059 0.219 0.1767 0.103 0.0557 0.0836 0.0975
Nuclear Periphery 0.0082 0.0097 0.0097 0.0193 0.0087 0.0096 0.0107 0.0056 0.0083 0.0123 0.0085 0.0074 0.0117 0.0065 0.012 0.011 0.0083 0.0089
Nucleolus 0.0011 0.0013 0.0015 0.0026 0.0011 0.0016 0.0015 0.0013 0.0011 0.0017 0.0008 0.0014 0.0007 0.0006 0.0012 0.0008 0.0006 0.001
Nucleus 0.1512 0.1576 0.1947 0.2338 0.1753 0.1941 0.1521 0.0959 0.1306 0.1588 0.1304 0.1831 0.1512 0.0708 0.149 0.2062 0.1202 0.1751
Peroxisomes 0.0027 0.0015 0.0022 0.0005 0.0003 0.0008 0.0013 0.0015 0.0044 0.0009 0.0003 0.001 0.0003 0.0013 0.0009 0.0004 0.0003 0.0005
Punctate Nuclear 0.0215 0.0342 0.0312 0.0559 0.0377 0.0682 0.023 0.0183 0.0162 0.0558 0.028 0.0384 0.0161 0.0102 0.0224 0.0171 0.0309 0.0328
Vacuole 0.0029 0.002 0.0016 0.0027 0.0019 0.0015 0.003 0.0025 0.0023 0.0024 0.0018 0.0016 0.0041 0.0035 0.0051 0.0042 0.0027 0.0034
Vacuole Periphery 0.0005 0.0004 0.0004 0.0007 0.0003 0.0005 0.0006 0.0003 0.0004 0.0005 0.0004 0.0003 0.0008 0.0005 0.001 0.0007 0.0005 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1455 12.4542 16.6349 15.1868 10.838 13.9242 18.2591 21.2477 22.9216 17.974
Translational Efficiency 1.384 1.2124 0.7912 0.9932 1.371 1.295 1.0448 0.8913 0.8255 1.0282

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); homolog of human NMNAT; NMA2 has a paralog, NMA1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (84%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nma2

Nma2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nma2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available