Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0.05 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.72 0.63 0.87 0.91 0.86 0.81 0.71 0.69 0.62 0.61 0.54 0.63 0.96 0.96 0.97 0.9 0.79 0.78 0.39 0.3 0.32 0.46 0.4 0.49
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0.51 0 0 0 0.14 0.32 0.3 0.43 0.39 0.63 0.4 0 0 0 0.07 0.07 0.08 0.41 0.43 0.47 0.35 0.35 0.28
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0 0.07 0.07 0.07 0.07 0.06 0.07 0 0.07 0 0 0 0 0.05 0.05 0 0 0 0 0.06 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 1 0 0 0 1 0 0 0 0 3 0 0 0 1 0 3 3 1 6 11 13
Bud 3 3 0 5 4 6 10 11 8 11 8 17 3 4 3 0 3 2 0 0 0 0 6 7
Bud Neck 0 0 3 10 21 14 12 17 9 15 2 16 11 10 9 1 0 0 3 0 0 2 6 15
Bud Site 0 0 0 0 0 0 2 1 3 3 2 5 0 1 0 0 0 2
Cell Periphery 0 0 2 2 1 0 0 0 0 3 0 3 6 1 5 2 6 4 1 0 0 2 5 8
Cytoplasm 142 45 39 187 320 285 335 239 222 230 109 249 394 475 719 102 170 144 91 30 31 97 208 266
Endoplasmic Reticulum 3 6 1 0 0 0 0 0 0 0 0 0 5 1 1 5 12 20 4 1 0 1 3 5
Endosome 0 0 0 0 3 2 2 0 2 2 0 1 1 2 11 2 10 8 6 2 3 7 20 8
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 0 0 1 1 2
Mitochondria 65 36 2 7 13 49 151 103 155 148 128 159 2 3 2 8 16 14 95 43 46 73 183 154
Nucleus 0 1 1 0 2 0 1 3 1 3 5 1 0 2 1 2 0 0 0 0 0 0 1 0
Nuclear Periphery 0 2 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 1 0 0 6 0 2 2 0 0 0 0 2 1
SpindlePole 5 0 3 8 27 26 31 23 22 28 3 28 1 5 8 2 10 10 7 3 3 5 30 30
Vac/Vac Membrane 0 1 0 1 6 4 0 1 2 0 2 10 1 3 9 4 15 6 6 7 2 6 20 12
Unique Cell Count 196 71 45 205 370 350 469 344 358 379 202 398 411 493 744 113 216 185 231 101 99 214 521 546
Labelled Cell Count 219 94 54 222 397 386 545 399 425 443 259 490 427 507 775 129 246 213 231 101 99 214 521 546


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 3.9 4.2 4.3 4.6 3.6 3.3 3.7 3.2 3.3 3.0 3.3 4.6 4.5 4.6 5.1 5.4 5.2 4.3 4.6 4.8
Std Deviation (1e-4) 0.6 0.9 0.7 1.4 1.8 1.5 1.4 0.9 1.4 0.9 1.1 0.9 0.7 0.8 1.0 1.4 1.5 1.9 1.3 1.2 1.3
Intensity Change (Log2) 0.03 0.15 -0.19 -0.34 -0.17 -0.38 -0.33 -0.5 -0.32 0.15 0.12 0.15 0.28 0.38 0.33 0.04 0.15 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0722 -0.4163 -0.6703 -1.0785 -0.7497 -0.6898 -0.0029 0.0448 0.2225 0.2213 -0.0005 0.2344 -0.6355 -0.9396 -0.9742 -0.9425 -0.7083 -0.9575
Actin 0.0227 0.0023 0.0071 0.0025 0.0068 0.0084 0.0382 0.0014 0.0227 0.0195 0.022 0.0158 0.0332 0.0026 0.0108 0.0075 0.0879 0.0044
Bud 0.001 0.0024 0.0034 0.0023 0.0059 0.0007 0.0022 0.0034 0.0034 0.0016 0.0017 0.0004 0.0014 0.0029 0.0015 0.002 0.0042 0.0008
Bud Neck 0.0034 0.001 0.0047 0.0026 0.0086 0.0085 0.0141 0.001 0.008 0.0105 0.0016 0.0051 0.0091 0.0012 0.0037 0.0138 0.0237 0.0049
Bud Periphery 0.0019 0.0018 0.0062 0.0025 0.0221 0.0009 0.0059 0.0078 0.0055 0.0041 0.0044 0.0008 0.0042 0.0047 0.0062 0.0037 0.0097 0.0018
Bud Site 0.0052 0.0224 0.0222 0.0181 0.0045 0.0006 0.0088 0.0155 0.0141 0.006 0.0081 0.0007 0.013 0.0393 0.0146 0.001 0.0117 0.0006
Cell Periphery 0.0006 0.0012 0.0012 0.0004 0.001 0.0003 0.0021 0.0008 0.0011 0.0006 0.0004 0.0002 0.0036 0.001 0.0022 0.0003 0.0015 0.0004
Cytoplasm 0.0931 0.1566 0.1058 0.0822 0.1077 0.1389 0.1242 0.1551 0.137 0.104 0.1173 0.2082 0.1247 0.2643 0.1825 0.1723 0.0862 0.2087
Cytoplasmic Foci 0.1572 0.1047 0.1272 0.1121 0.1564 0.1434 0.1098 0.1309 0.0821 0.1046 0.1526 0.1228 0.1426 0.1204 0.1101 0.1238 0.1559 0.1575
Eisosomes 0.0006 0.0007 0.0004 0.0005 0.0006 0.0003 0.0008 0.0003 0.0003 0.0003 0.0003 0.0002 0.0014 0.001 0.001 0.0003 0.0013 0.0002
Endoplasmic Reticulum 0.0037 0.006 0.0037 0.0018 0.0028 0.0037 0.0056 0.0028 0.0024 0.003 0.0035 0.003 0.0138 0.0062 0.0058 0.0036 0.0039 0.0037
Endosome 0.0994 0.0674 0.0877 0.0604 0.0564 0.0637 0.0621 0.0629 0.0492 0.0696 0.0783 0.061 0.0999 0.101 0.0782 0.1365 0.163 0.0991
Golgi 0.0116 0.0129 0.0133 0.0038 0.0144 0.0082 0.0107 0.0062 0.0066 0.0159 0.0161 0.0083 0.015 0.0181 0.018 0.0051 0.0181 0.0135
Lipid Particles 0.0478 0.008 0.0151 0.005 0.0544 0.0209 0.0262 0.0153 0.0086 0.0162 0.0362 0.0094 0.0402 0.0064 0.0154 0.0113 0.0543 0.0139
Mitochondria 0.0409 0.0397 0.0498 0.0055 0.0597 0.0321 0.0164 0.0363 0.031 0.0572 0.0568 0.037 0.0392 0.0275 0.0591 0.031 0.053 0.0676
None 0.464 0.5389 0.508 0.6691 0.4011 0.5089 0.5082 0.5252 0.5842 0.5017 0.4486 0.4885 0.3794 0.3796 0.4536 0.4349 0.1952 0.3801
Nuclear Periphery 0.0021 0.0009 0.0041 0.0024 0.001 0.0044 0.0108 0.0014 0.001 0.0131 0.0027 0.0043 0.0138 0.002 0.0069 0.007 0.0048 0.0045
Nucleolus 0.0026 0.0013 0.0014 0.0023 0.0021 0.0013 0.0023 0.0027 0.0016 0.0022 0.0022 0.0006 0.0019 0.001 0.0013 0.0011 0.0048 0.0011
Nucleus 0.0021 0.0027 0.0028 0.0031 0.0023 0.0034 0.0043 0.0033 0.0025 0.0038 0.0022 0.0017 0.013 0.0017 0.0027 0.003 0.0032 0.0075
Peroxisomes 0.023 0.0174 0.0237 0.0083 0.045 0.0336 0.02 0.0111 0.0186 0.0462 0.0268 0.0204 0.0246 0.0033 0.0131 0.0175 0.0812 0.0122
Punctate Nuclear 0.0045 0.0037 0.0051 0.0081 0.0419 0.0109 0.0164 0.0034 0.0032 0.0102 0.0102 0.0045 0.0107 0.0023 0.0026 0.006 0.0204 0.0076
Vacuole 0.0106 0.0068 0.0052 0.0056 0.004 0.005 0.0085 0.0111 0.0151 0.0075 0.0058 0.0054 0.0127 0.0112 0.0083 0.015 0.0115 0.0071
Vacuole Periphery 0.002 0.0011 0.0018 0.0015 0.0011 0.0018 0.0022 0.002 0.0019 0.0025 0.0021 0.0016 0.0027 0.0023 0.0025 0.0035 0.0043 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0614 5.9962 2.2247 8.0349 7.2473 3.352 6.0628 7.5019 7.2979 8.8558
Translational Efficiency 0.525 0.7655 1.4589 0.5195 0.6268 1.7592 0.5957 0.4073 0.6397 0.6924

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Eat1

Eat1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eat1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available