Standard name | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 1 | 6 | 11 | 13 |
Bud | 3 | 3 | 0 | 5 | 4 | 6 | 10 | 11 | 8 | 11 | 8 | 17 | 3 | 4 | 3 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 6 | 7 |
Bud Neck | 0 | 0 | 3 | 10 | 21 | 14 | 12 | 17 | 9 | 15 | 2 | 16 | 11 | 10 | 9 | 1 | 0 | 0 | 3 | 0 | 0 | 2 | 6 | 15 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 3 | 2 | 5 | 0 | 1 | 0 | 0 | 0 | 2 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 6 | 1 | 5 | 2 | 6 | 4 | 1 | 0 | 0 | 2 | 5 | 8 |
Cytoplasm | 142 | 45 | 39 | 187 | 320 | 285 | 335 | 239 | 222 | 230 | 109 | 249 | 394 | 475 | 719 | 102 | 170 | 144 | 91 | 30 | 31 | 97 | 208 | 266 |
Endoplasmic Reticulum | 3 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 1 | 5 | 12 | 20 | 4 | 1 | 0 | 1 | 3 | 5 |
Endosome | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 0 | 2 | 2 | 0 | 1 | 1 | 2 | 11 | 2 | 10 | 8 | 6 | 2 | 3 | 7 | 20 | 8 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 1 | 1 | 2 |
Mitochondria | 65 | 36 | 2 | 7 | 13 | 49 | 151 | 103 | 155 | 148 | 128 | 159 | 2 | 3 | 2 | 8 | 16 | 14 | 95 | 43 | 46 | 73 | 183 | 154 |
Nucleus | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 3 | 1 | 3 | 5 | 1 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 6 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 1 |
SpindlePole | 5 | 0 | 3 | 8 | 27 | 26 | 31 | 23 | 22 | 28 | 3 | 28 | 1 | 5 | 8 | 2 | 10 | 10 | 7 | 3 | 3 | 5 | 30 | 30 |
Vac/Vac Membrane | 0 | 1 | 0 | 1 | 6 | 4 | 0 | 1 | 2 | 0 | 2 | 10 | 1 | 3 | 9 | 4 | 15 | 6 | 6 | 7 | 2 | 6 | 20 | 12 |
Unique Cell Count | 196 | 71 | 45 | 205 | 370 | 350 | 469 | 344 | 358 | 379 | 202 | 398 | 411 | 493 | 744 | 113 | 216 | 185 | 231 | 101 | 99 | 214 | 521 | 546 |
Labelled Cell Count | 219 | 94 | 54 | 222 | 397 | 386 | 545 | 399 | 425 | 443 | 259 | 490 | 427 | 507 | 775 | 129 | 246 | 213 | 231 | 101 | 99 | 214 | 521 | 546 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.0 | 3.9 | 4.2 | 4.3 | 4.6 | 3.6 | 3.3 | 3.7 | 3.2 | 3.3 | 3.0 | 3.3 | 4.6 | 4.5 | 4.6 | 5.1 | 5.4 | 5.2 | 4.3 | 4.6 | 4.8 |
Std Deviation (1e-4) | 0.6 | 0.9 | 0.7 | 1.4 | 1.8 | 1.5 | 1.4 | 0.9 | 1.4 | 0.9 | 1.1 | 0.9 | 0.7 | 0.8 | 1.0 | 1.4 | 1.5 | 1.9 | 1.3 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.15 | -0.19 | -0.34 | -0.17 | -0.38 | -0.33 | -0.5 | -0.32 | 0.15 | 0.12 | 0.15 | 0.28 | 0.38 | 0.33 | 0.04 | 0.15 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.0722 | -0.4163 | -0.6703 | -1.0785 | -0.7497 | -0.6898 | -0.0029 | 0.0448 | 0.2225 | 0.2213 | -0.0005 | 0.2344 | -0.6355 | -0.9396 | -0.9742 | -0.9425 | -0.7083 | -0.9575 |
Actin | 0.0227 | 0.0023 | 0.0071 | 0.0025 | 0.0068 | 0.0084 | 0.0382 | 0.0014 | 0.0227 | 0.0195 | 0.022 | 0.0158 | 0.0332 | 0.0026 | 0.0108 | 0.0075 | 0.0879 | 0.0044 |
Bud | 0.001 | 0.0024 | 0.0034 | 0.0023 | 0.0059 | 0.0007 | 0.0022 | 0.0034 | 0.0034 | 0.0016 | 0.0017 | 0.0004 | 0.0014 | 0.0029 | 0.0015 | 0.002 | 0.0042 | 0.0008 |
Bud Neck | 0.0034 | 0.001 | 0.0047 | 0.0026 | 0.0086 | 0.0085 | 0.0141 | 0.001 | 0.008 | 0.0105 | 0.0016 | 0.0051 | 0.0091 | 0.0012 | 0.0037 | 0.0138 | 0.0237 | 0.0049 |
Bud Periphery | 0.0019 | 0.0018 | 0.0062 | 0.0025 | 0.0221 | 0.0009 | 0.0059 | 0.0078 | 0.0055 | 0.0041 | 0.0044 | 0.0008 | 0.0042 | 0.0047 | 0.0062 | 0.0037 | 0.0097 | 0.0018 |
Bud Site | 0.0052 | 0.0224 | 0.0222 | 0.0181 | 0.0045 | 0.0006 | 0.0088 | 0.0155 | 0.0141 | 0.006 | 0.0081 | 0.0007 | 0.013 | 0.0393 | 0.0146 | 0.001 | 0.0117 | 0.0006 |
Cell Periphery | 0.0006 | 0.0012 | 0.0012 | 0.0004 | 0.001 | 0.0003 | 0.0021 | 0.0008 | 0.0011 | 0.0006 | 0.0004 | 0.0002 | 0.0036 | 0.001 | 0.0022 | 0.0003 | 0.0015 | 0.0004 |
Cytoplasm | 0.0931 | 0.1566 | 0.1058 | 0.0822 | 0.1077 | 0.1389 | 0.1242 | 0.1551 | 0.137 | 0.104 | 0.1173 | 0.2082 | 0.1247 | 0.2643 | 0.1825 | 0.1723 | 0.0862 | 0.2087 |
Cytoplasmic Foci | 0.1572 | 0.1047 | 0.1272 | 0.1121 | 0.1564 | 0.1434 | 0.1098 | 0.1309 | 0.0821 | 0.1046 | 0.1526 | 0.1228 | 0.1426 | 0.1204 | 0.1101 | 0.1238 | 0.1559 | 0.1575 |
Eisosomes | 0.0006 | 0.0007 | 0.0004 | 0.0005 | 0.0006 | 0.0003 | 0.0008 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0014 | 0.001 | 0.001 | 0.0003 | 0.0013 | 0.0002 |
Endoplasmic Reticulum | 0.0037 | 0.006 | 0.0037 | 0.0018 | 0.0028 | 0.0037 | 0.0056 | 0.0028 | 0.0024 | 0.003 | 0.0035 | 0.003 | 0.0138 | 0.0062 | 0.0058 | 0.0036 | 0.0039 | 0.0037 |
Endosome | 0.0994 | 0.0674 | 0.0877 | 0.0604 | 0.0564 | 0.0637 | 0.0621 | 0.0629 | 0.0492 | 0.0696 | 0.0783 | 0.061 | 0.0999 | 0.101 | 0.0782 | 0.1365 | 0.163 | 0.0991 |
Golgi | 0.0116 | 0.0129 | 0.0133 | 0.0038 | 0.0144 | 0.0082 | 0.0107 | 0.0062 | 0.0066 | 0.0159 | 0.0161 | 0.0083 | 0.015 | 0.0181 | 0.018 | 0.0051 | 0.0181 | 0.0135 |
Lipid Particles | 0.0478 | 0.008 | 0.0151 | 0.005 | 0.0544 | 0.0209 | 0.0262 | 0.0153 | 0.0086 | 0.0162 | 0.0362 | 0.0094 | 0.0402 | 0.0064 | 0.0154 | 0.0113 | 0.0543 | 0.0139 |
Mitochondria | 0.0409 | 0.0397 | 0.0498 | 0.0055 | 0.0597 | 0.0321 | 0.0164 | 0.0363 | 0.031 | 0.0572 | 0.0568 | 0.037 | 0.0392 | 0.0275 | 0.0591 | 0.031 | 0.053 | 0.0676 |
None | 0.464 | 0.5389 | 0.508 | 0.6691 | 0.4011 | 0.5089 | 0.5082 | 0.5252 | 0.5842 | 0.5017 | 0.4486 | 0.4885 | 0.3794 | 0.3796 | 0.4536 | 0.4349 | 0.1952 | 0.3801 |
Nuclear Periphery | 0.0021 | 0.0009 | 0.0041 | 0.0024 | 0.001 | 0.0044 | 0.0108 | 0.0014 | 0.001 | 0.0131 | 0.0027 | 0.0043 | 0.0138 | 0.002 | 0.0069 | 0.007 | 0.0048 | 0.0045 |
Nucleolus | 0.0026 | 0.0013 | 0.0014 | 0.0023 | 0.0021 | 0.0013 | 0.0023 | 0.0027 | 0.0016 | 0.0022 | 0.0022 | 0.0006 | 0.0019 | 0.001 | 0.0013 | 0.0011 | 0.0048 | 0.0011 |
Nucleus | 0.0021 | 0.0027 | 0.0028 | 0.0031 | 0.0023 | 0.0034 | 0.0043 | 0.0033 | 0.0025 | 0.0038 | 0.0022 | 0.0017 | 0.013 | 0.0017 | 0.0027 | 0.003 | 0.0032 | 0.0075 |
Peroxisomes | 0.023 | 0.0174 | 0.0237 | 0.0083 | 0.045 | 0.0336 | 0.02 | 0.0111 | 0.0186 | 0.0462 | 0.0268 | 0.0204 | 0.0246 | 0.0033 | 0.0131 | 0.0175 | 0.0812 | 0.0122 |
Punctate Nuclear | 0.0045 | 0.0037 | 0.0051 | 0.0081 | 0.0419 | 0.0109 | 0.0164 | 0.0034 | 0.0032 | 0.0102 | 0.0102 | 0.0045 | 0.0107 | 0.0023 | 0.0026 | 0.006 | 0.0204 | 0.0076 |
Vacuole | 0.0106 | 0.0068 | 0.0052 | 0.0056 | 0.004 | 0.005 | 0.0085 | 0.0111 | 0.0151 | 0.0075 | 0.0058 | 0.0054 | 0.0127 | 0.0112 | 0.0083 | 0.015 | 0.0115 | 0.0071 |
Vacuole Periphery | 0.002 | 0.0011 | 0.0018 | 0.0015 | 0.0011 | 0.0018 | 0.0022 | 0.002 | 0.0019 | 0.0025 | 0.0021 | 0.0016 | 0.0027 | 0.0023 | 0.0025 | 0.0035 | 0.0043 | 0.0028 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0614 | 5.9962 | 2.2247 | 8.0349 | 7.2473 | 3.352 | 6.0628 | 7.5019 | 7.2979 | 8.8558 |
Translational Efficiency | 0.525 | 0.7655 | 1.4589 | 0.5195 | 0.6268 | 1.7592 | 0.5957 | 0.4073 | 0.6397 | 0.6924 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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