Standard name
Human Ortholog
Description Highly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0.14 0 0 0 0 0 0.05 0 0 0.08 0.11
Bud 0 0 0.09 0.05 0 0.05 0 0 – 0 0.06 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0 0 0 – 0 0 0 0.05 0 0.06 0.11 0.06 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.12 0 0 0 0 0 0 – 0 0 0 0 0.19 0.08 0.06 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0.1 0 0.09 0.13 0 0.06 0.06 0.08 0.07 0.06 0.06 0.05
Golgi 0.07 0.1 0 0 0 0 0 0 – 0 0 0 0.2 0.29 0.32 0.31 0.41 0.43 0.13 0.15 0.06 0.09 0.13 0.14
Mitochondria 0.88 0.88 0.94 0.93 0.9 0.94 0.95 0.93 – 0.93 0.96 0.97 0.63 0.47 0.45 0.08 0.09 0.07 0.66 0.62 0.68 0.63 0.56 0.52
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0.08 0 0.05 0.14 0.09 0.06 0.07 0 0.11 0.07 0.09 0.06
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.07 0 0 0.07 0 0.06 0.07 – 0.07 0.06 0 0.22 0.12 0.16 0.16 0.22 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 4 3 1 1 2 0 1 – 0 0 3 4 22 5 2 0 2 3 6 8 6 18 31
Bud 2 3 27 18 18 34 22 25 – 24 43 37 2 3 4 7 2 1 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2
Bud Site 0 0 0 1 1 4 13 12 – 5 9 12 0 0 0 1 0 0 – – – – – –
Cell Periphery 3 2 4 12 4 6 2 5 – 7 6 5 3 6 4 5 0 3 0 0 1 0 0 0
Cytoplasm 4 3 12 18 33 23 22 22 – 24 18 13 5 4 12 10 2 8 1 0 1 0 0 1
Endoplasmic Reticulum 8 14 11 11 9 18 9 14 – 19 6 9 0 29 16 5 1 5 1 1 1 2 3 6
Endosome 3 0 0 4 13 2 1 6 – 0 2 0 10 3 18 11 1 3 12 10 23 10 13 14
Golgi 13 11 2 3 3 0 0 0 – 3 0 1 20 45 64 27 13 23 26 19 20 15 30 40
Mitochondria 170 100 296 334 529 644 825 851 – 778 731 917 62 72 92 7 3 4 131 80 214 106 135 148
Nucleus 3 0 1 1 3 7 9 14 – 3 4 4 0 1 0 1 0 1 0 0 0 0 0 0
Nuclear Periphery 6 0 4 2 14 17 27 33 – 44 17 34 0 3 1 0 2 0 1 0 0 2 1 1
Nucleolus 1 0 0 0 8 21 38 56 – 17 16 22 0 0 0 0 1 0 0 0 0 0 1 0
Peroxisomes 3 0 5 6 6 1 2 9 – 4 5 4 8 4 11 12 3 3 14 4 34 12 21 17
SpindlePole 1 0 2 0 5 7 3 10 – 7 11 4 2 0 2 3 1 2 1 0 1 0 0 1
Vac/Vac Membrane 21 8 11 8 41 30 56 63 – 60 47 39 22 18 32 14 7 8 3 3 2 5 8 11
Unique Cell Count 193 114 315 360 586 688 865 915 833 762 944 98 153 203 87 32 54 201 130 315 170 241 283
Labelled Cell Count 242 145 378 419 688 817 1029 1121 995 915 1104 138 210 261 105 36 63 201 130 315 170 241 283


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.2 4.7 4.7 5.0 4.9 4.5 4.8 – 4.8 4.9 4.8 7.6 7.3 7.3 7.5 7.2 7.5 7.7 7.9 8.2
Std Deviation (1e-4) 0.8 1.4 1.1 1.0 1.2 1.2 1.1 1.2 – 1.2 1.2 1.1 1.6 1.0 0.9 1.7 1.3 1.6 1.6 1.6 1.7
Intensity Change (Log2) – – – -0.02 0.07 0.06 -0.09 0.02 – 0.01 0.04 0.02 0.68 0.62 0.62 0.66 0.59 0.67 0.7 0.74 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 6.1 0 0 0 0
Bud 0 -3.6 -2.2 0 0 – 0 -1.8 0 -2.2 -2.7 -3.1 -0.2 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 1.2 -0.4 -1.2 -1.3 – -0.8 -1.3 -2.7 0 -0.7 1.1 0 0 0
Endoplasmic Reticulum 0 -1.9 -0.8 0 0 – 0 0 0 0 5.6 2.2 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 5.4 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 7.6 9.7 10.3 9.7 0 0
Mitochondria 0 -1.9 -0.2 0 0 – 0 1.4 0 -7.8 -11.6 -12.5 -16.5 -13.2 -15.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1.1 1.2 0 0 – 0 1.0 0 0 0 0 0 0 0
Nucleolus 0 0 3.1 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 2.5 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.0 2.2 0.6 2.0 2.2 – 2.3 1.8 0.5 6.0 3.5 4.9 4.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7445 1.0998 1.0615 0.8986 0.5764 0.9894 2.7758 3.2722 3.3231 3.2799 2.3701 3.4259 0.8996 1.1005 0.9475 1.0469 0.8425 0.9481
Actin 0.0294 0.0349 0.0213 0.0065 0.0018 0.0126 0.0256 0.0138 0.0201 0.0502 0.0191 0.0222 0.0232 0.0259 0.0274 0.011 0.0085 0.0162
Bud 0.0007 0.0028 0.0011 0.0033 0.0039 0.0004 0.0006 0.0009 0.0012 0.0018 0.0005 0.0008 0.0006 0.0011 0.0007 0.0008 0.0047 0.0004
Bud Neck 0.0017 0.0012 0.0028 0.0006 0.0008 0.0008 0.0007 0.001 0.0014 0.0006 0.0024 0.0043 0.0018 0.0028 0.0016 0.0212 0.0071 0.0016
Bud Periphery 0.0015 0.0053 0.0038 0.0057 0.0052 0.0007 0.0009 0.0014 0.0027 0.0033 0.0011 0.002 0.0011 0.0013 0.0011 0.0024 0.0074 0.0008
Bud Site 0.005 0.0121 0.0085 0.0075 0.0172 0.0005 0.0018 0.0122 0.0042 0.0011 0.0015 0.0017 0.0037 0.0124 0.0059 0.0018 0.0197 0.0005
Cell Periphery 0.0015 0.0005 0.0017 0.0004 0.0012 0.0002 0.0003 0.0004 0.0015 0.0002 0.0003 0.0007 0.0007 0.0003 0.0007 0.0009 0.0013 0.0002
Cytoplasm 0.0094 0.0042 0.0054 0.0016 0.0018 0.0046 0.0034 0.0096 0.01 0.0031 0.0021 0.0069 0.0053 0.0081 0.0038 0.0017 0.0045 0.0015
Cytoplasmic Foci 0.0587 0.062 0.0426 0.0196 0.0562 0.0273 0.0467 0.0534 0.0374 0.0536 0.0382 0.0431 0.0636 0.0631 0.0329 0.0411 0.0454 0.0251
Eisosomes 0.0043 0.0005 0.0017 0.0002 0.0004 0.0003 0.0005 0.0004 0.0027 0.0003 0.0005 0.0006 0.001 0.0002 0.001 0.0002 0.0002 0.0003
Endoplasmic Reticulum 0.0013 0.0006 0.0007 0.0004 0.0002 0.0009 0.0012 0.0012 0.0008 0.0004 0.0003 0.001 0.0017 0.0014 0.0018 0.0001 0.0055 0.0009
Endosome 0.1031 0.1177 0.051 0.0278 0.0654 0.0566 0.1282 0.0943 0.0639 0.085 0.0856 0.0785 0.094 0.1065 0.0677 0.0485 0.126 0.0658
Golgi 0.095 0.147 0.08 0.0351 0.0722 0.075 0.1672 0.1459 0.0989 0.0895 0.1811 0.1116 0.1129 0.1299 0.1006 0.0702 0.1142 0.1048
Lipid Particles 0.0442 0.0122 0.0187 0.004 0.0357 0.0165 0.0286 0.0153 0.0093 0.0107 0.043 0.0081 0.0297 0.0197 0.0096 0.0034 0.0082 0.0165
Mitochondria 0.4955 0.5087 0.6553 0.7703 0.6535 0.7306 0.537 0.5711 0.6628 0.6066 0.5895 0.6268 0.5561 0.544 0.6669 0.7276 0.5852 0.701
None 0.0265 0.0012 0.0219 0.0003 0.0021 0.0067 0.0132 0.0188 0.0171 0.0028 0.0007 0.015 0.0179 0.0124 0.0079 0.0003 0.0019 0.0042
Nuclear Periphery 0.0057 0.0002 0.0013 0.0003 0.0002 0.0003 0.0004 0.0007 0.0002 0.0001 0.0003 0.0017 0.0042 0.001 0.001 0 0.0066 0.0005
Nucleolus 0.0032 0.0004 0.0011 0.0001 0.0015 0.0004 0.0003 0.0002 0.0013 0.0002 0.0004 0.0003 0.0007 0.0006 0.0006 0.0001 0.0006 0.0001
Nucleus 0.0016 0.0005 0.0008 0.0003 0.0005 0.0012 0.0003 0.0005 0.001 0.0002 0.0002 0.0005 0.0007 0.0008 0.0003 0.0001 0.001 0.0002
Peroxisomes 0.0947 0.0769 0.0729 0.1099 0.0708 0.0596 0.0341 0.0532 0.0551 0.0845 0.029 0.0659 0.0722 0.0584 0.0604 0.0661 0.0375 0.054
Punctate Nuclear 0.0022 0.0002 0.0023 0.0001 0.004 0.0005 0.0003 0.0002 0.0006 0.0008 0.0013 0.0019 0.0015 0.0003 0.0001 0.0001 0.0023 0.0002
Vacuole 0.0102 0.0066 0.0028 0.0044 0.003 0.0021 0.0049 0.0034 0.0059 0.0025 0.0013 0.0034 0.0037 0.0057 0.0045 0.0011 0.0065 0.002
Vacuole Periphery 0.0044 0.004 0.002 0.0016 0.0022 0.0023 0.0038 0.0021 0.002 0.0025 0.0015 0.0029 0.0035 0.0042 0.0036 0.0012 0.0059 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.1323 11.7749 13.4423 16.2033 13.1369 14.1773 21.7506 19.1615 17.2618 21.3857
Translational Efficiency 1.382 0.974 0.9489 0.9139 0.9551 1.3719 0.8612 0.9105 1.356 0.696

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Highly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain
Localization
Cell Percentages mitochondrion (72%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Msp1

Msp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available