Standard name
Human Ortholog
Description Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; regulates septum assembly, and may directly phosphorylate Bni4p; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.08 0.09 0.06 0.08 0.07 0 0.05 0.06 0.05 0 0.08 0.22 0.26 0.12 0.19 0.15 0 0 0 0.4 0.35 0.36
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 0 0 0 0.16 0.35 0.19 0.47 0.21 0.5 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.87 0.81 0.84 0.84 0.79 0.79 0.7 0.84 0.71 0.81 0.73 0.83 0.82 0.61 0.62 0.57 0.49 0.53 0.78 0.74 0.7 0.22 0.2 0.18
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 0.05 0 0.1 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0.15 0.1 0.07 0.13 0.07 0.05 0 0 0 0 0 0 0.11 0.09 0.31 0.32 0.38 0 0.07 0.1 0.09 0.13 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 5 6
Bud 0 1 0 0 0 2 8 6 3 4 4 4 0 0 0 0 0 0 4 6 3 6 12 11
Bud Neck 0 0 1 0 0 1 1 0 0 3 2 1 0 0 0 0 0 0 0 0 0 1 4 6
Bud Site 0 0 0 0 3 4 6 10 13 12 16 12 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 10 13 21 24 28 32 21 19 22 17 9 10 37 39 13 23 5 2 4 3 38 58 75
Endoplasmic Reticulum 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 7 0 1 0 1 0 3 6
Endosome 0 3 0 1 9 4 2 3 1 0 0 0 4 7 8 1 0 0 4 4 3 4 8 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 2 2 2 1 2 5
Mitochondria 64 5 3 9 13 54 154 95 172 81 155 85 3 1 1 1 5 1 5 4 6 0 3 1
Nucleus 237 169 138 200 316 273 307 421 259 315 227 304 99 103 93 59 59 18 232 189 133 20 33 36
Nuclear Periphery 0 0 0 0 0 0 2 1 0 1 2 0 0 0 1 0 0 0 1 1 0 0 1 0
Nucleolus 13 7 1 0 0 5 13 17 20 16 30 16 5 7 0 1 2 0 20 16 7 2 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
SpindlePole 0 0 3 5 5 2 1 4 3 4 0 1 1 3 2 0 0 0 3 3 4 5 5 7
Vac/Vac Membrane 10 31 16 17 53 24 22 12 7 11 7 9 5 18 13 32 38 13 12 17 19 8 21 31
Unique Cell Count 273 208 165 239 399 345 437 499 365 388 312 365 120 168 149 104 120 34 296 255 190 96 167 208
Labelled Cell Count 332 226 176 254 425 398 548 590 497 469 460 441 127 177 159 109 135 37 296 255 190 96 167 208


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 7.4 6.4 6.4 6.4 5.5 5.2 5.8 4.7 5.6 4.8 5.5 7.9 8.2 7.7 9.5 10.0 9.0 7.8 8.6 9.1
Std Deviation (1e-4) 0.6 1.2 1.2 1.3 1.1 1.3 1.3 1.3 1.1 1.4 1.1 1.0 3.3 2.9 2.4 1.5 2.4 1.5 2.6 3.2 3.5
Intensity Change (Log2) -0.01 -0.02 -0.23 -0.31 -0.15 -0.45 -0.19 -0.41 -0.23 0.29 0.36 0.25 0.56 0.64 0.48 0.28 0.41 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 -0.8 0.1 -0.2 -1.9 -1.2 -1.0 -1.0 -2.9 0.1 3.6 4.4 1.2 2.8 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.6 8.3 5.4 0 5.7 0 6.2 0 0 0 0 0 0
Nucleus 0 -1.2 -1.2 -3.3 0.2 -3.1 -0.7 -2.7 -0.1 -0.3 -4.6 -4.3 -4.9 -6.2 -4.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2.2 0 2.3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.9 1.2 -1.1 -2.1 -4.0 -4.1 -3.4 -3.6 -3.6 -1.8 0.3 -0.3 4.4 4.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9692 4.6383 4.0759 3.6245 3.7475 4.2944 3.5978 4.2293 3.7272 3.4116 2.9619 3.6498 3.8872 3.4847 3.1963 2.942 3.2753 2.9013
Actin 0.0376 0 0.0008 0.0476 0.0578 0.0025 0.0147 0.0003 0.0071 0.0077 0.0003 0.0015 0.017 0 0.02 0.0023 0.0045 0.001
Bud 0.0005 0 0.0001 0.0041 0.0009 0.0002 0.001 0.0003 0.0004 0.0006 0.0004 0.0001 0.0008 0 0.0001 0.0002 0.0003 0.0002
Bud Neck 0.0008 0.0001 0.0007 0.0023 0.002 0.0034 0.003 0.0004 0.0018 0.0093 0.0014 0.0018 0.003 0.0001 0.0007 0.0002 0.0008 0.0019
Bud Periphery 0.0006 0 0.0003 0.0099 0.0011 0.0003 0.002 0.0003 0.0005 0.0012 0.001 0.0002 0.0036 0 0.0001 0.0002 0.0005 0.0003
Bud Site 0.0025 0.0001 0.0007 0.0069 0.0042 0.0007 0.0116 0.0005 0.0027 0.0013 0.0006 0.0002 0.0011 0.0001 0.0004 0.0002 0.0005 0.0002
Cell Periphery 0.0002 0 0.0001 0.0003 0.0001 0.0001 0.0004 0 0.0001 0.0003 0.0001 0 0.0004 0 0 0 0.0001 0
Cytoplasm 0.006 0.0009 0.006 0.001 0.0056 0.0017 0.0032 0.0008 0.0073 0.0157 0.0074 0.0018 0.0165 0.0006 0.0043 0.0044 0.0042 0.0011
Cytoplasmic Foci 0.0115 0 0.0014 0.0231 0.0137 0.0027 0.0144 0.0003 0.0056 0.0058 0.0032 0.0013 0.0047 0 0.0003 0.0021 0.0015 0.0029
Eisosomes 0.0005 0 0 0.0003 0.0001 0 0.0002 0 0.0001 0.0001 0 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0019 0 0.001 0.001 0.0029 0.0018 0.002 0.0002 0.0015 0.0025 0.0005 0.0003 0.0051 0 0.0008 0.0009 0.002 0.0008
Endosome 0.0234 0 0.0043 0.0069 0.088 0.0206 0.0183 0.0009 0.0129 0.0176 0.0032 0.0035 0.0168 0 0.0017 0.0054 0.0139 0.0031
Golgi 0.011 0 0.0005 0.0106 0.0256 0.005 0.0061 0.0001 0.0021 0.0105 0.0003 0.0024 0.0032 0 0.0004 0.0005 0.0085 0.0006
Lipid Particles 0.0171 0 0.001 0.0125 0.0082 0.0033 0.0109 0.0001 0.0058 0.0051 0.002 0.0024 0.0016 0 0.0002 0.0003 0.0062 0.0002
Mitochondria 0.0093 0.0001 0.0061 0.0164 0.0269 0.0146 0.0087 0.0005 0.0029 0.0113 0.0022 0.0084 0.004 0.0002 0.0004 0.0011 0.0155 0.001
None 0.0047 0 0.0002 0.0005 0.0516 0.0015 0.0017 0.0001 0.0005 0.0021 0.0145 0.0002 0.002 0 0.0005 0.0283 0.0006 0.0032
Nuclear Periphery 0.0081 0.0022 0.0028 0.0034 0.0096 0.0054 0.014 0.0032 0.0071 0.0096 0.0082 0.0022 0.0297 0.0021 0.012 0.0075 0.0437 0.0053
Nucleolus 0.0055 0.0107 0.0059 0.0052 0.0435 0.018 0.0117 0.0057 0.0071 0.0101 0.0207 0.0165 0.0085 0.0054 0.0068 0.0023 0.0086 0.0103
Nucleus 0.8365 0.9848 0.9579 0.8057 0.6189 0.9117 0.8361 0.9716 0.9026 0.8387 0.9084 0.9489 0.8556 0.9771 0.9367 0.939 0.8755 0.9517
Peroxisomes 0.0076 0 0.0056 0.0332 0.0268 0.0007 0.0091 0.0001 0.0098 0.0024 0.0017 0.0012 0.0005 0 0.0001 0.0007 0.0005 0.0001
Punctate Nuclear 0.0122 0.0007 0.0007 0.0065 0.007 0.0042 0.0206 0.014 0.0126 0.0456 0.0221 0.0064 0.0169 0.0141 0.0138 0.0039 0.0082 0.015
Vacuole 0.0014 0.0001 0.0031 0.0016 0.0034 0.0011 0.0083 0.0004 0.0072 0.0016 0.0013 0.0003 0.0063 0.0001 0.0003 0.0003 0.002 0.0008
Vacuole Periphery 0.0009 0 0.0006 0.0009 0.0021 0.0006 0.0022 0.0001 0.0022 0.0008 0.0005 0.0003 0.0025 0 0.0004 0.0003 0.0024 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.948 4.4552 11.9014 16.6562 17.4465 7.9696 14.64 12.7693 15.0767 19.7661
Translational Efficiency 0.6671 2.2799 0.8284 0.732 0.8082 1.1287 0.7339 0.6862 0.686 0.7134

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; regulates septum assembly, and may directly phosphorylate Bni4p; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Kss1

Kss1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kss1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available