Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 3 | 1 | 2 | 3 | 0 | 0 | 0 | – | 0 | 1 | 3 | 2 | 2 | 6 | 13 | 9 |
Bud Neck | 0 | 0 | 3 | 1 | 0 | 0 | 2 | – | 1 | 3 | 3 | 2 | 0 | 4 | 1 | – | 0 | 0 | 1 | 1 | 1 | 3 | 4 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 2 | 5 | 6 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 105 | 111 | 85 | 45 | 6 | 84 | 45 | – | 41 | 48 | 38 | 28 | 122 | 186 | 216 | – | 72 | 127 | 87 | 83 | 56 | 76 | 94 | 156 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2 | 0 | 0 | 1 | – | 3 | 0 | 0 | 2 | 1 | 1 | 1 | – | 17 | 19 | 0 | 1 | 1 | 0 | 3 | 5 |
Endosome | 1 | 1 | 3 | 0 | 0 | 3 | 3 | – | 0 | 0 | 0 | 0 | 2 | 1 | 3 | – | 12 | 7 | 3 | 6 | 1 | 0 | 8 | 7 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 13 | 32 | 6 | 21 | 5 | 55 | 34 | – | 127 | 101 | 142 | 101 | 0 | 5 | 1 | – | 15 | 15 | 9 | 8 | 14 | 9 | 19 | 31 |
Nucleus | 79 | 65 | 40 | 35 | 10 | 59 | 54 | – | 63 | 56 | 70 | 60 | 61 | 110 | 89 | – | 89 | 127 | 117 | 94 | 52 | 61 | 73 | 39 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 2 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 4 | 8 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 1 | 2 | 0 | 0 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 3 | 3 | – | 2 | 15 | 11 | 9 | 3 | 14 | 25 | 22 |
Vac/Vac Membrane | 91 | 66 | 19 | 6 | 0 | 28 | 12 | – | 5 | 8 | 18 | 10 | 19 | 31 | 60 | – | 78 | 52 | 81 | 66 | 52 | 79 | 104 | 129 |
Unique Cell Count | 261 | 233 | 150 | 96 | 20 | 199 | 132 | 180 | 169 | 204 | 151 | 196 | 324 | 351 | 250 | 319 | 325 | 282 | 194 | 270 | 381 | 439 | ||
Labelled Cell Count | 290 | 278 | 158 | 110 | 21 | 231 | 152 | 246 | 225 | 279 | 207 | 206 | 342 | 374 | 286 | 367 | 325 | 282 | 194 | 270 | 381 | 439 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 3.8 | 5.0 | 4.4 | 3.7 | 4.0 | 4.3 | – | 3.3 | 3.5 | 3.4 | 3.4 | 5.5 | 5.5 | 5.2 | 4.2 | 5.3 | 5.2 | 6.4 | 6.7 | 6.4 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.6 | 1.4 | 0.7 | 1.3 | 1.2 | – | 0.7 | 0.7 | 1.0 | 0.6 | 1.6 | 1.4 | 1.1 | 1.0 | 1.6 | 1.1 | 2.1 | 2.3 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.42 | -0.32 | -0.22 | – | -0.61 | -0.53 | -0.56 | -0.54 | 0.13 | 0.14 | 0.05 | -0.26 | 0.09 | 0.05 | 0.35 | 0.43 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 4.2 | 1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.1064 | -0.6073 | -0.8955 | -0.9159 | -1.1527 | -0.7519 | 4.6325 | 4.9135 | 3.9733 | 4.0951 | 3.7183 | 4.1386 | 1.8939 | 1.5421 | 1.4739 | 3.0323 | 1.908 | 1.3219 |
Actin | 0.0137 | 0.0002 | 0.0019 | 0.0007 | 0.0001 | 0.0007 | 0.0307 | 0.0009 | 0.0018 | 0.0271 | 0.0382 | 0.0033 | 0.0592 | 0.0007 | 0.0076 | 0.0996 | 0.0369 | 0.0002 |
Bud | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0046 | 0.003 | 0.0016 | 0.0031 | 0.0002 | 0.0007 | 0.0012 | 0.0003 | 0.0003 | 0.0059 | 0.0002 | 0 |
Bud Neck | 0.0008 | 0.0004 | 0.0009 | 0.0009 | 0.0003 | 0.0015 | 0.0164 | 0.0018 | 0.004 | 0.0014 | 0.0023 | 0.0029 | 0.0022 | 0.0004 | 0.0015 | 0.0012 | 0.0028 | 0.001 |
Bud Periphery | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0.0054 | 0.0081 | 0.0015 | 0.0132 | 0.0006 | 0.0035 | 0.0012 | 0.0005 | 0.0004 | 0.0073 | 0.0002 | 0 |
Bud Site | 0.0005 | 0.0015 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0068 | 0.0121 | 0.0055 | 0.0031 | 0.0003 | 0.0002 | 0.0075 | 0.001 | 0.008 | 0.0164 | 0.0014 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0002 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 |
Cytoplasm | 0.1016 | 0.1277 | 0.0756 | 0.0428 | 0.0435 | 0.136 | 0.1252 | 0.1951 | 0.1376 | 0.0383 | 0.1338 | 0.1926 | 0.0775 | 0.1321 | 0.0756 | 0.0339 | 0.1012 | 0.1381 |
Cytoplasmic Foci | 0.0125 | 0.0033 | 0.0144 | 0.0019 | 0.0053 | 0.0093 | 0.0189 | 0.0075 | 0.0101 | 0.0071 | 0.0234 | 0.0064 | 0.0186 | 0.008 | 0.0102 | 0.0415 | 0.0402 | 0.0026 |
Eisosomes | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0006 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0006 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0027 | 0.0016 | 0.0019 | 0.0062 | 0.0005 | 0.0013 | 0.0058 | 0.0026 | 0.003 | 0.0034 | 0.0022 | 0.002 | 0.0029 | 0.0036 | 0.0015 | 0.0052 | 0.0036 | 0.0017 |
Endosome | 0.005 | 0.0018 | 0.0045 | 0.0019 | 0.0008 | 0.0047 | 0.0187 | 0.004 | 0.0042 | 0.0108 | 0.0031 | 0.0021 | 0.0147 | 0.0085 | 0.0095 | 0.0752 | 0.0208 | 0.0017 |
Golgi | 0.0008 | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0007 | 0.0036 | 0.0003 | 0.0018 | 0.0057 | 0.0007 | 0.0001 | 0.0118 | 0.0014 | 0.0059 | 0.0375 | 0.0201 | 0 |
Lipid Particles | 0.0028 | 0.0011 | 0.0021 | 0.0005 | 0.0001 | 0.0022 | 0.0062 | 0.0004 | 0.0019 | 0.0081 | 0.0011 | 0.0003 | 0.0278 | 0.0019 | 0.0075 | 0.0214 | 0.0273 | 0.0002 |
Mitochondria | 0.0006 | 0.0005 | 0.0013 | 0.0005 | 0.0002 | 0.004 | 0.0049 | 0.0013 | 0.0052 | 0.0077 | 0.0004 | 0.0005 | 0.0107 | 0.0007 | 0.0262 | 0.0332 | 0.0042 | 0.0003 |
None | 0.5867 | 0.6468 | 0.6031 | 0.5182 | 0.792 | 0.5251 | 0.4755 | 0.5261 | 0.5918 | 0.6328 | 0.6213 | 0.5336 | 0.5784 | 0.6644 | 0.7133 | 0.4855 | 0.6795 | 0.6753 |
Nuclear Periphery | 0.0158 | 0.0087 | 0.0181 | 0.0526 | 0.002 | 0.0063 | 0.0309 | 0.0086 | 0.0088 | 0.0056 | 0.0019 | 0.0048 | 0.0233 | 0.0054 | 0.0029 | 0.0087 | 0.0021 | 0.0048 |
Nucleolus | 0.0033 | 0.0032 | 0.0052 | 0.003 | 0.0016 | 0.0039 | 0.0034 | 0.0034 | 0.0024 | 0.0023 | 0.0008 | 0.0029 | 0.0056 | 0.0042 | 0.0012 | 0.0006 | 0.0008 | 0.0022 |
Nucleus | 0.2194 | 0.1767 | 0.1984 | 0.3554 | 0.146 | 0.2641 | 0.2025 | 0.1684 | 0.1919 | 0.2111 | 0.1607 | 0.2071 | 0.1241 | 0.1566 | 0.1155 | 0.0929 | 0.0034 | 0.1608 |
Peroxisomes | 0.0009 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0026 | 0.0044 | 0.0054 | 0.0045 | 0.0081 | 0.0004 | 0.0003 | 0.0157 | 0.0008 | 0.0056 | 0.0221 | 0.0168 | 0.0001 |
Punctate Nuclear | 0.0296 | 0.0241 | 0.0677 | 0.0132 | 0.0068 | 0.0359 | 0.0253 | 0.048 | 0.0181 | 0.0062 | 0.0069 | 0.0347 | 0.0085 | 0.0064 | 0.0046 | 0.0074 | 0.0367 | 0.01 |
Vacuole | 0.002 | 0.0014 | 0.0013 | 0.0009 | 0.0004 | 0.0008 | 0.0072 | 0.0025 | 0.0025 | 0.0028 | 0.0007 | 0.0015 | 0.006 | 0.0026 | 0.0019 | 0.0021 | 0.001 | 0.0006 |
Vacuole Periphery | 0.0004 | 0.0003 | 0.0006 | 0.0007 | 0.0001 | 0.0002 | 0.0029 | 0.0004 | 0.0005 | 0.0012 | 0.0001 | 0.0003 | 0.0022 | 0.0004 | 0.0003 | 0.0014 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.931 | 8.4616 | 8.9698 | 17.0078 | 17.532 | 5.4059 | 13.4443 | 15.2608 | 15.7168 | 19.6261 |
Translational Efficiency | 0.5511 | 0.79 | 0.8196 | 0.4101 | 0.351 | 2.2357 | 0.5404 | 0.4876 | 0.5053 | 0.537 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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